polypeptide Search Results


serum ghrelin  (Shanghai Korain Biotech Co Ltd)
94
Shanghai Korain Biotech Co Ltd serum ghrelin
Serum Ghrelin, supplied by Shanghai Korain Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/serum ghrelin/product/Shanghai Korain Biotech Co Ltd
Average 94 stars, based on 1 article reviews
serum ghrelin - by Bioz Stars, 2026-03
94/100 stars
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dlk  (Carna Inc)
94
Carna Inc dlk
Dlk, supplied by Carna Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dlk/product/Carna Inc
Average 94 stars, based on 1 article reviews
dlk - by Bioz Stars, 2026-03
94/100 stars
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atp1a1  (Proteintech)
96
Proteintech atp1a1
Atp1a1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/atp1a1/product/Proteintech
Average 96 stars, based on 1 article reviews
atp1a1 - by Bioz Stars, 2026-03
96/100 stars
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anti tff2  (Proteintech)
93
Proteintech anti tff2
Anti Tff2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti tff2/product/Proteintech
Average 93 stars, based on 1 article reviews
anti tff2 - by Bioz Stars, 2026-03
93/100 stars
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ferritin light chain  (Proteintech)
96
Proteintech ferritin light chain
FIGURE 4 BBR protected HaCaT cells against AGEs-induced ferroptosis partially through NRF2 activity. (A) Representative Western blot experiments illustrating NRF2 protein expression in HaCaT cells after transfection with NRF2 siRNA. (B) The mock-, NRF2 siRNA- or Con siRNA-transfected HaCaT cells were cultured with 150 μg/mL AGEs and 5 μM BBR for 48 h, and cell viability was examined using CCK-8 assays. (C) The relative levels of MDA were measured in HaCaT cells after treatment. (D) Representative images and quantitative analysis of the protein levels of GPX4 and FTL in HaCaT cells. The results are expressed as the mean ± SD. Data are representative of three independent experiments. *P ≤0.05, **P ≤0.01. AGEs, advanced glycation end products; BBR, berberine; Con siRNA, control siRNA; FTL, <t>ferritin</t> <t>light</t> <t>chain;</t> GPX4, glutathione peroxidase 4; MDA, malondialdehyde; NRF2, nuclear factor E2-related factor 2; ns, no significance.
Ferritin Light Chain, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ferritin light chain/product/Proteintech
Average 96 stars, based on 1 article reviews
ferritin light chain - by Bioz Stars, 2026-03
96/100 stars
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cox4  (Proteintech)
96
Proteintech cox4
FIGURE 4 BBR protected HaCaT cells against AGEs-induced ferroptosis partially through NRF2 activity. (A) Representative Western blot experiments illustrating NRF2 protein expression in HaCaT cells after transfection with NRF2 siRNA. (B) The mock-, NRF2 siRNA- or Con siRNA-transfected HaCaT cells were cultured with 150 μg/mL AGEs and 5 μM BBR for 48 h, and cell viability was examined using CCK-8 assays. (C) The relative levels of MDA were measured in HaCaT cells after treatment. (D) Representative images and quantitative analysis of the protein levels of GPX4 and FTL in HaCaT cells. The results are expressed as the mean ± SD. Data are representative of three independent experiments. *P ≤0.05, **P ≤0.01. AGEs, advanced glycation end products; BBR, berberine; Con siRNA, control siRNA; FTL, <t>ferritin</t> <t>light</t> <t>chain;</t> GPX4, glutathione peroxidase 4; MDA, malondialdehyde; NRF2, nuclear factor E2-related factor 2; ns, no significance.
Cox4, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cox4/product/Proteintech
Average 96 stars, based on 1 article reviews
cox4 - by Bioz Stars, 2026-03
96/100 stars
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epithelial receptor  (OriGene)
92
OriGene epithelial receptor
FIGURE 4 BBR protected HaCaT cells against AGEs-induced ferroptosis partially through NRF2 activity. (A) Representative Western blot experiments illustrating NRF2 protein expression in HaCaT cells after transfection with NRF2 siRNA. (B) The mock-, NRF2 siRNA- or Con siRNA-transfected HaCaT cells were cultured with 150 μg/mL AGEs and 5 μM BBR for 48 h, and cell viability was examined using CCK-8 assays. (C) The relative levels of MDA were measured in HaCaT cells after treatment. (D) Representative images and quantitative analysis of the protein levels of GPX4 and FTL in HaCaT cells. The results are expressed as the mean ± SD. Data are representative of three independent experiments. *P ≤0.05, **P ≤0.01. AGEs, advanced glycation end products; BBR, berberine; Con siRNA, control siRNA; FTL, <t>ferritin</t> <t>light</t> <t>chain;</t> GPX4, glutathione peroxidase 4; MDA, malondialdehyde; NRF2, nuclear factor E2-related factor 2; ns, no significance.
Epithelial Receptor, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/epithelial receptor/product/OriGene
Average 92 stars, based on 1 article reviews
epithelial receptor - by Bioz Stars, 2026-03
92/100 stars
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failure od26278 31 y lisinopril liver ndri male 78 y t2dm none cardiac od2072 untreated arrest liver origene  (OriGene)
93
OriGene failure od26278 31 y lisinopril liver ndri male 78 y t2dm none cardiac od2072 untreated arrest liver origene
FIGURE 4 BBR protected HaCaT cells against AGEs-induced ferroptosis partially through NRF2 activity. (A) Representative Western blot experiments illustrating NRF2 protein expression in HaCaT cells after transfection with NRF2 siRNA. (B) The mock-, NRF2 siRNA- or Con siRNA-transfected HaCaT cells were cultured with 150 μg/mL AGEs and 5 μM BBR for 48 h, and cell viability was examined using CCK-8 assays. (C) The relative levels of MDA were measured in HaCaT cells after treatment. (D) Representative images and quantitative analysis of the protein levels of GPX4 and FTL in HaCaT cells. The results are expressed as the mean ± SD. Data are representative of three independent experiments. *P ≤0.05, **P ≤0.01. AGEs, advanced glycation end products; BBR, berberine; Con siRNA, control siRNA; FTL, <t>ferritin</t> <t>light</t> <t>chain;</t> GPX4, glutathione peroxidase 4; MDA, malondialdehyde; NRF2, nuclear factor E2-related factor 2; ns, no significance.
Failure Od26278 31 Y Lisinopril Liver Ndri Male 78 Y T2dm None Cardiac Od2072 Untreated Arrest Liver Origene, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/failure od26278 31 y lisinopril liver ndri male 78 y t2dm none cardiac od2072 untreated arrest liver origene/product/OriGene
Average 93 stars, based on 1 article reviews
failure od26278 31 y lisinopril liver ndri male 78 y t2dm none cardiac od2072 untreated arrest liver origene - by Bioz Stars, 2026-03
93/100 stars
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rabbit  (Boster Bio)
92
Boster Bio rabbit
FIGURE 4 BBR protected HaCaT cells against AGEs-induced ferroptosis partially through NRF2 activity. (A) Representative Western blot experiments illustrating NRF2 protein expression in HaCaT cells after transfection with NRF2 siRNA. (B) The mock-, NRF2 siRNA- or Con siRNA-transfected HaCaT cells were cultured with 150 μg/mL AGEs and 5 μM BBR for 48 h, and cell viability was examined using CCK-8 assays. (C) The relative levels of MDA were measured in HaCaT cells after treatment. (D) Representative images and quantitative analysis of the protein levels of GPX4 and FTL in HaCaT cells. The results are expressed as the mean ± SD. Data are representative of three independent experiments. *P ≤0.05, **P ≤0.01. AGEs, advanced glycation end products; BBR, berberine; Con siRNA, control siRNA; FTL, <t>ferritin</t> <t>light</t> <t>chain;</t> GPX4, glutathione peroxidase 4; MDA, malondialdehyde; NRF2, nuclear factor E2-related factor 2; ns, no significance.
Rabbit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit/product/Boster Bio
Average 92 stars, based on 1 article reviews
rabbit - by Bioz Stars, 2026-03
92/100 stars
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antibody against skeletal myosin fast  (Boster Bio)
93
Boster Bio antibody against skeletal myosin fast
FIGURE 4 BBR protected HaCaT cells against AGEs-induced ferroptosis partially through NRF2 activity. (A) Representative Western blot experiments illustrating NRF2 protein expression in HaCaT cells after transfection with NRF2 siRNA. (B) The mock-, NRF2 siRNA- or Con siRNA-transfected HaCaT cells were cultured with 150 μg/mL AGEs and 5 μM BBR for 48 h, and cell viability was examined using CCK-8 assays. (C) The relative levels of MDA were measured in HaCaT cells after treatment. (D) Representative images and quantitative analysis of the protein levels of GPX4 and FTL in HaCaT cells. The results are expressed as the mean ± SD. Data are representative of three independent experiments. *P ≤0.05, **P ≤0.01. AGEs, advanced glycation end products; BBR, berberine; Con siRNA, control siRNA; FTL, <t>ferritin</t> <t>light</t> <t>chain;</t> GPX4, glutathione peroxidase 4; MDA, malondialdehyde; NRF2, nuclear factor E2-related factor 2; ns, no significance.
Antibody Against Skeletal Myosin Fast, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibody against skeletal myosin fast/product/Boster Bio
Average 93 stars, based on 1 article reviews
antibody against skeletal myosin fast - by Bioz Stars, 2026-03
93/100 stars
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pancreatic polypeptide  (Novus Biologicals)
92
Novus Biologicals pancreatic polypeptide
FIGURE 4 BBR protected HaCaT cells against AGEs-induced ferroptosis partially through NRF2 activity. (A) Representative Western blot experiments illustrating NRF2 protein expression in HaCaT cells after transfection with NRF2 siRNA. (B) The mock-, NRF2 siRNA- or Con siRNA-transfected HaCaT cells were cultured with 150 μg/mL AGEs and 5 μM BBR for 48 h, and cell viability was examined using CCK-8 assays. (C) The relative levels of MDA were measured in HaCaT cells after treatment. (D) Representative images and quantitative analysis of the protein levels of GPX4 and FTL in HaCaT cells. The results are expressed as the mean ± SD. Data are representative of three independent experiments. *P ≤0.05, **P ≤0.01. AGEs, advanced glycation end products; BBR, berberine; Con siRNA, control siRNA; FTL, <t>ferritin</t> <t>light</t> <t>chain;</t> GPX4, glutathione peroxidase 4; MDA, malondialdehyde; NRF2, nuclear factor E2-related factor 2; ns, no significance.
Pancreatic Polypeptide, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pancreatic polypeptide/product/Novus Biologicals
Average 92 stars, based on 1 article reviews
pancreatic polypeptide - by Bioz Stars, 2026-03
92/100 stars
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γ tubulin 555  (ProSci Incorporated)
93
ProSci Incorporated γ tubulin 555
FIGURE 4 BBR protected HaCaT cells against AGEs-induced ferroptosis partially through NRF2 activity. (A) Representative Western blot experiments illustrating NRF2 protein expression in HaCaT cells after transfection with NRF2 siRNA. (B) The mock-, NRF2 siRNA- or Con siRNA-transfected HaCaT cells were cultured with 150 μg/mL AGEs and 5 μM BBR for 48 h, and cell viability was examined using CCK-8 assays. (C) The relative levels of MDA were measured in HaCaT cells after treatment. (D) Representative images and quantitative analysis of the protein levels of GPX4 and FTL in HaCaT cells. The results are expressed as the mean ± SD. Data are representative of three independent experiments. *P ≤0.05, **P ≤0.01. AGEs, advanced glycation end products; BBR, berberine; Con siRNA, control siRNA; FTL, <t>ferritin</t> <t>light</t> <t>chain;</t> GPX4, glutathione peroxidase 4; MDA, malondialdehyde; NRF2, nuclear factor E2-related factor 2; ns, no significance.
γ Tubulin 555, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/γ tubulin 555/product/ProSci Incorporated
Average 93 stars, based on 1 article reviews
γ tubulin 555 - by Bioz Stars, 2026-03
93/100 stars
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Image Search Results


FIGURE 4 BBR protected HaCaT cells against AGEs-induced ferroptosis partially through NRF2 activity. (A) Representative Western blot experiments illustrating NRF2 protein expression in HaCaT cells after transfection with NRF2 siRNA. (B) The mock-, NRF2 siRNA- or Con siRNA-transfected HaCaT cells were cultured with 150 μg/mL AGEs and 5 μM BBR for 48 h, and cell viability was examined using CCK-8 assays. (C) The relative levels of MDA were measured in HaCaT cells after treatment. (D) Representative images and quantitative analysis of the protein levels of GPX4 and FTL in HaCaT cells. The results are expressed as the mean ± SD. Data are representative of three independent experiments. *P ≤0.05, **P ≤0.01. AGEs, advanced glycation end products; BBR, berberine; Con siRNA, control siRNA; FTL, ferritin light chain; GPX4, glutathione peroxidase 4; MDA, malondialdehyde; NRF2, nuclear factor E2-related factor 2; ns, no significance.

Journal: Experimental biology and medicine (Maywood, N.J.)

Article Title: Berberine alleviates AGEs-induced ferroptosis by activating NRF2 in the skin of diabetic mice.

doi: 10.3389/ebm.2024.10280

Figure Lengend Snippet: FIGURE 4 BBR protected HaCaT cells against AGEs-induced ferroptosis partially through NRF2 activity. (A) Representative Western blot experiments illustrating NRF2 protein expression in HaCaT cells after transfection with NRF2 siRNA. (B) The mock-, NRF2 siRNA- or Con siRNA-transfected HaCaT cells were cultured with 150 μg/mL AGEs and 5 μM BBR for 48 h, and cell viability was examined using CCK-8 assays. (C) The relative levels of MDA were measured in HaCaT cells after treatment. (D) Representative images and quantitative analysis of the protein levels of GPX4 and FTL in HaCaT cells. The results are expressed as the mean ± SD. Data are representative of three independent experiments. *P ≤0.05, **P ≤0.01. AGEs, advanced glycation end products; BBR, berberine; Con siRNA, control siRNA; FTL, ferritin light chain; GPX4, glutathione peroxidase 4; MDA, malondialdehyde; NRF2, nuclear factor E2-related factor 2; ns, no significance.

Article Snippet: After blocking for 1 h at room temperature, the membranes underwent an overnight incubation with primary antibodies against the following proteins: glutathione peroxidase 4 (GPX4; 1:1000; 67763-1-Ig; Proteintech, Wuhan, China), ferritin light chain (FTL; 1:1000; 10727-1-AP; Proteintech), NRF2 (1:800; 12721; Cell Signaling Technology, Danvers, United States), α-tubulin (1: 20,000; HRP-66031; Proteintech), or lamin B1 (1:10,000; 12987- 1-AP; Proteintech).

Techniques: Activity Assay, Western Blot, Expressing, Transfection, Cell Culture, CCK-8 Assay, Control