sirt1 (Proteintech)

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Proteintech sirt1
Sirt1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 322 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal/SIRT1+Polyclonal+antibody/pm42929872-128-104-106
Average 96 stars, based on 322 article reviews

sirt1 - by Bioz Stars, 2026-11

96/100 stars

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Incubation:

Article Title: Characterization of a new mutation of mitochondrial ND6 gene in hepatocellular carcinoma and its effects on respiratory complex I
Article Snippet: .. Membranes were saturated by incubation with 5% BSA or 5% non-fat dry milk, depending on the antibody, in TBS-T [1X TBS supplemented with 0.1% Tween 20] for 1 h. Blots were incubated with the primary antibody overnight at 4 °C [α-ND6: 1:500 polyclonal (MBS8518686, MyBioSource), 1:500 polyclonal (NBP1-70650, Novus Biologicals); α-NDUFS1: 1:500 monoclonal (ab169540, Abcam); α-NDUFS3: 1:10000 monoclonal (68066-1-Ig, Proteintech); α-NDUFB11: 1:2000 polyclonal (16720-1-AP, Proteintech); α-NDUFA1: 1: 500 monoclonal (ab176563, Abcam); α-ND1: 1:1000 polyclonal (19703-1-AP, Proteintech); α-ND2: 1:1000 polyclonal (19704-1-AP, Proteintech); α-ND5: 1:500 polyclonal (ab138136, Abcam); α-OxPhos: 1:1000 monoclonal (ab110411, Abcam)]. .. Membranes were washed three times for 5 min with TBS-T, incubated with the secondary antibody (IRDye 800, IRDye 680 LI-COR Biosciences) for 2 h, and rewashed three times for 5 min with TBS-T. Blots were acquired using the Odyssey DLx scanner (LI-COR Biosciences), and densitometric analysis was performed with ImageStudio software (LI-COR Biosciences).

Article Title: Characterization of a new mutation of mitochondrial ND6 gene in hepatocellular carcinoma and its effects on respiratory complex I.
Article Snippet: .. Membranes were saturated by incubation with 5% BSA or 5% non-fat dry milk, depending on the antibody, in TBS-T [1X TBS supplemented with 0.1% Tween 20] for 1 h. Blots were incubated with the primary antibody overnight at 4 °C [α-ND6: 1:500 polyclonal (MBS8518686, MyBioSource), 1:500 polyclonal (NBP1-70650, Novus Biologicals); α-NDUFS1: 1:500 monoclonal (ab169540, Abcam); α-NDUFS3: 1:10000 monoclonal (68066-1-Ig, Proteintech); α-NDUFB11: 1:2000 polyclonal (16720-1- AP, Proteintech); α-NDUFA1: 1: 500 monoclonal (ab176563, Abcam); α-ND1: 1:1000 polyclonal (19703-1-AP, Proteintech); α-ND2: 1:1000 polyclonal (19704-1-AP, Proteintech); α-ND5: 1:500 polyclonal (ab138136, Abcam); α-OxPhos: 1:1000 monoclonal (ab110411, Abcam)]. .. Membranes were washed three times for 5 min with TBS-T, incubated with the secondary antibody (IRDye 800, IRDye 680 LI-COR Biosciences) for 2 h, and rewashed three times for 5 min with TBS-T. Blots were acquired using the Odyssey DLx scanner (LI-COR Biosciences), and densitometric analysis was performed with ImageStudio software (LI-COR Biosciences).

Immunohistochemistry:

Article Title: ONECUT2 reprograms neuroendocrine fate and is an actionable therapeutic target in small cell lung cancer.
Article Snippet: Human cell lines were obtained from the American Type Culture Collection (ATCC) and tested negative for mycoplasma contamination. .. Anti-OC2, rabbit, polyclonal (Sigma, HPA057058, lot no. A104348) (1:100) was used for the IHC studies; antiOC2, rabbit, polyclonal (Sigma, HPA057058, lot no. A104348) (1:1000) and anti-OC2, rabbit, polyclonal (Proteintech, 21916–1-AP, lot no. 00114937) (1:1000) were used for western blot. .. The following antibodies were also used for western blot: anti-ASCL1 (Abcam, AB211327, lot no. 12) (1:1000), anti-NEUROD1 (Abcam, AB109224, lot no. 1016722–2) (1:1000), anti-YAP (Cell Signaling, 14074, lot no. 5) (1:1000), anti-c-MYC (Cell Signaling, 5605, lot no.16) (1:1000), anti-β-actin, mouse, monoclonal (Sigma A5441, lot no. 0000126950) (1:2000); antirabbit IgG, HRP-linked (Cell Signaling 7074, lot no. 28) (1:5000); anti-mouse IgG, HRP-linked (GE-Healthcare NA931, lot no. 17271474) (1:5000).

Article Title: ONECUT2 reprograms neuroendocrine fate and is an actionable therapeutic target in small cell lung cancer
Article Snippet: Human cell lines were obtained from the American Type Culture Collection (ATCC) and tested negative for mycoplasma contamination. .. Anti-OC2, rabbit, polyclonal (Sigma, HPA057058, lot no. A104348) (1:100) was used for the IHC studies; anti-OC2, rabbit, polyclonal (Sigma, HPA057058, lot no. A104348) (1:1000) and anti-OC2, rabbit, polyclonal (Proteintech, 21916–1-AP, lot no. 00114937) (1:1000) were used for western blot. .. The following antibodies were also used for western blot: anti-ASCL1 (Abcam, AB211327 , lot no. 12) (1:1000), anti-NEUROD1 (Abcam, AB109224 , lot no. 1016722–2) (1:1000), anti-YAP (Cell Signaling, 14074, lot no. 5) (1:1000), anti-c-MYC (Cell Signaling, 5605, lot no.16) (1:1000), anti-β-actin, mouse, monoclonal (Sigma A5441, lot no. 0000126950) (1:2000); anti-rabbit IgG, HRP-linked (Cell Signaling 7074, lot no. 28) (1:5000); anti-mouse IgG, HRP-linked (GE-Healthcare NA931, lot no. 17271474) (1:5000).

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sirt1 (Proteintech)

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