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poly i c  (InvivoGen)


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    InvivoGen poly i c
    Free reactive oxygen species affect basal and LPS induced gene expression on lipid modified matrices. A Bar charts represent relative gene expression of TNFA and IL1A normalized to B2M showing mean ± SD, n = 4. THP-1 cells were activated with 15 nM PMA, cultured on modified matrices for 24 h, and then stimulated for 2 h with human TNFα (100 ng/ml), Flagellin (0.25 μg/ml, TLR5), FSL-1 (0.5 μg/ml, TLR2, TLR6), and <t>Poly(I:C)</t> (20 μg/ml <t>TLR3),</t> respectively. B, C Bar charts show relative expression levels of macrophages treated with 5 mM N-Acetylcysteine (NAC) immediately after seeding and cultured for 24 h, followed by stimulation with 500 ng/ml LPS for 2 h, mean ± SD, n = 4. Asterisks indicate statistical significance (* p < 0.05; ** p < 0.01; *** p < 0.005; **** p < 0.001), and exact p-values are shown for borderline significance determined by one-way ANOVA.
    Poly I C, supplied by InvivoGen, used in various techniques. Bioz Stars score: 98/100, based on 4988 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/poly i c/product/InvivoGen
    Average 98 stars, based on 4988 article reviews
    poly i c - by Bioz Stars, 2026-04
    98/100 stars

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    1) Product Images from "Modification of the dermal matrix by senescence associated lipids and its functional consequence"

    Article Title: Modification of the dermal matrix by senescence associated lipids and its functional consequence

    Journal: Redox Biology

    doi: 10.1016/j.redox.2026.104069

    Free reactive oxygen species affect basal and LPS induced gene expression on lipid modified matrices. A Bar charts represent relative gene expression of TNFA and IL1A normalized to B2M showing mean ± SD, n = 4. THP-1 cells were activated with 15 nM PMA, cultured on modified matrices for 24 h, and then stimulated for 2 h with human TNFα (100 ng/ml), Flagellin (0.25 μg/ml, TLR5), FSL-1 (0.5 μg/ml, TLR2, TLR6), and Poly(I:C) (20 μg/ml TLR3), respectively. B, C Bar charts show relative expression levels of macrophages treated with 5 mM N-Acetylcysteine (NAC) immediately after seeding and cultured for 24 h, followed by stimulation with 500 ng/ml LPS for 2 h, mean ± SD, n = 4. Asterisks indicate statistical significance (* p < 0.05; ** p < 0.01; *** p < 0.005; **** p < 0.001), and exact p-values are shown for borderline significance determined by one-way ANOVA.
    Figure Legend Snippet: Free reactive oxygen species affect basal and LPS induced gene expression on lipid modified matrices. A Bar charts represent relative gene expression of TNFA and IL1A normalized to B2M showing mean ± SD, n = 4. THP-1 cells were activated with 15 nM PMA, cultured on modified matrices for 24 h, and then stimulated for 2 h with human TNFα (100 ng/ml), Flagellin (0.25 μg/ml, TLR5), FSL-1 (0.5 μg/ml, TLR2, TLR6), and Poly(I:C) (20 μg/ml TLR3), respectively. B, C Bar charts show relative expression levels of macrophages treated with 5 mM N-Acetylcysteine (NAC) immediately after seeding and cultured for 24 h, followed by stimulation with 500 ng/ml LPS for 2 h, mean ± SD, n = 4. Asterisks indicate statistical significance (* p < 0.05; ** p < 0.01; *** p < 0.005; **** p < 0.001), and exact p-values are shown for borderline significance determined by one-way ANOVA.

    Techniques Used: Gene Expression, Modification, Cell Culture, Expressing



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    Free reactive oxygen species affect basal and LPS induced gene expression on lipid modified matrices. A Bar charts represent relative gene expression of TNFA and IL1A normalized to B2M showing mean ± SD, n = 4. THP-1 cells were activated with 15 nM PMA, cultured on modified matrices for 24 h, and then stimulated for 2 h with human TNFα (100 ng/ml), Flagellin (0.25 μg/ml, TLR5), FSL-1 (0.5 μg/ml, TLR2, TLR6), and Poly(I:C) (20 μg/ml TLR3), respectively. B, C Bar charts show relative expression levels of macrophages treated with 5 mM N-Acetylcysteine (NAC) immediately after seeding and cultured for 24 h, followed by stimulation with 500 ng/ml LPS for 2 h, mean ± SD, n = 4. Asterisks indicate statistical significance (* p < 0.05; ** p < 0.01; *** p < 0.005; **** p < 0.001), and exact p-values are shown for borderline significance determined by one-way ANOVA.

    Journal: Redox Biology

    Article Title: Modification of the dermal matrix by senescence associated lipids and its functional consequence

    doi: 10.1016/j.redox.2026.104069

    Figure Lengend Snippet: Free reactive oxygen species affect basal and LPS induced gene expression on lipid modified matrices. A Bar charts represent relative gene expression of TNFA and IL1A normalized to B2M showing mean ± SD, n = 4. THP-1 cells were activated with 15 nM PMA, cultured on modified matrices for 24 h, and then stimulated for 2 h with human TNFα (100 ng/ml), Flagellin (0.25 μg/ml, TLR5), FSL-1 (0.5 μg/ml, TLR2, TLR6), and Poly(I:C) (20 μg/ml TLR3), respectively. B, C Bar charts show relative expression levels of macrophages treated with 5 mM N-Acetylcysteine (NAC) immediately after seeding and cultured for 24 h, followed by stimulation with 500 ng/ml LPS for 2 h, mean ± SD, n = 4. Asterisks indicate statistical significance (* p < 0.05; ** p < 0.01; *** p < 0.005; **** p < 0.001), and exact p-values are shown for borderline significance determined by one-way ANOVA.

    Article Snippet: In detail, human TNFα (InvitrogenThermo Fisher, USA) was added to a final concentration of 100 ng/ml, 0.25 μg/ml of Flagellin (TLR5, InvivoGen, France), 0.5 μg/ml of FSL-1 (TLR2, TLR6, InvivoGen) and 20 μg/ml of Poly(I:C) (TLR3, InvivoGen) [ , ].

    Techniques: Gene Expression, Modification, Cell Culture, Expressing