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phmb resistant acanthamoeba castellanii a castellanii trophozoites  (ATCC)


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    ATCC phmb resistant acanthamoeba castellanii a castellanii trophozoites
    Phmb Resistant Acanthamoeba Castellanii A Castellanii Trophozoites, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 149 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phmb resistant acanthamoeba castellanii a castellanii trophozoites/product/ATCC
    Average 96 stars, based on 149 article reviews
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    Antimicrobial activity of the tested compounds against P. aeruginosa strains assessed using a modified disk diffusion method. Results for diameters of growth inhibition zones (mm) (n = 10). (A) Representative zones of P857 strain growth inhibition after the treatment with Thyme Essential Oil (TEO). (B) Representative zones of P857 strain growth inhibition after the treatment with polyhexanide (PHMB). (C) The lines represent a mean value.

    Journal: Frontiers in Pharmacology

    Article Title: Toward essential oil stewardship: strain-resolved evaluation of thyme oil activity against Pseudomonas aeruginosa

    doi: 10.3389/fphar.2025.1659096

    Figure Lengend Snippet: Antimicrobial activity of the tested compounds against P. aeruginosa strains assessed using a modified disk diffusion method. Results for diameters of growth inhibition zones (mm) (n = 10). (A) Representative zones of P857 strain growth inhibition after the treatment with Thyme Essential Oil (TEO). (B) Representative zones of P857 strain growth inhibition after the treatment with polyhexanide (PHMB). (C) The lines represent a mean value.

    Article Snippet: The activity of TEO and PHMB against P. aeruginosa ATCC 15442 and P857 biofilms was confirmed also using a fluorescence microscope ( ).

    Techniques: Activity Assay, Modification, Diffusion-based Assay, Inhibition

    Antibiofilm activity of the tested compounds against P. aeruginosa strains (n = 10) expressed as a reduction (log 10 ) of biofilm cells. TEO, Thyme Essential Oil, PHMB, polyhexanide. The lines represent a mean value.

    Journal: Frontiers in Pharmacology

    Article Title: Toward essential oil stewardship: strain-resolved evaluation of thyme oil activity against Pseudomonas aeruginosa

    doi: 10.3389/fphar.2025.1659096

    Figure Lengend Snippet: Antibiofilm activity of the tested compounds against P. aeruginosa strains (n = 10) expressed as a reduction (log 10 ) of biofilm cells. TEO, Thyme Essential Oil, PHMB, polyhexanide. The lines represent a mean value.

    Article Snippet: The activity of TEO and PHMB against P. aeruginosa ATCC 15442 and P857 biofilms was confirmed also using a fluorescence microscope ( ).

    Techniques: Activity Assay

    Microscopic visualizations of the P. aeruginosa biofilm (covering the surface of a 24-well plate) stained with a LIVE/DEAD dye. ATCC 15442 strain’s untreated biofilm. (A) ATCC 15442 strain’s biofilm treated with Thyme Essential Oil. (B) ATCC 15442 strain’s biofilm treated with polyhexanide. (C) P857 strain’s untreated biofilm. (D) P857 strain’s biofilm treated with Thyme Essential Oil. (E) P857 strain’s biofilm treated with polyhexanide. (F) The red/orange color shows bacterial cells with altered/damaged cell walls; the green color indicates unaltered cell walls. Fluorescence microscope Etaluma 600 (magnification 4×). The well diameter was 15 mm.

    Journal: Frontiers in Pharmacology

    Article Title: Toward essential oil stewardship: strain-resolved evaluation of thyme oil activity against Pseudomonas aeruginosa

    doi: 10.3389/fphar.2025.1659096

    Figure Lengend Snippet: Microscopic visualizations of the P. aeruginosa biofilm (covering the surface of a 24-well plate) stained with a LIVE/DEAD dye. ATCC 15442 strain’s untreated biofilm. (A) ATCC 15442 strain’s biofilm treated with Thyme Essential Oil. (B) ATCC 15442 strain’s biofilm treated with polyhexanide. (C) P857 strain’s untreated biofilm. (D) P857 strain’s biofilm treated with Thyme Essential Oil. (E) P857 strain’s biofilm treated with polyhexanide. (F) The red/orange color shows bacterial cells with altered/damaged cell walls; the green color indicates unaltered cell walls. Fluorescence microscope Etaluma 600 (magnification 4×). The well diameter was 15 mm.

    Article Snippet: The activity of TEO and PHMB against P. aeruginosa ATCC 15442 and P857 biofilms was confirmed also using a fluorescence microscope ( ).

    Techniques: Staining, Fluorescence, Microscopy

    Visual representation of the data distribution for antimicrobial and antibiofilm activity of the tested compounds against genetically distinct groups of P. aeruginosa strains. Distribution of growth inhibition zones values (mm). (A,B) Distribution of Minimal Inhibitory Concentration (MIC) (%, v/v). (C,D) Distribution of biofilm cells reduction (%). (E,F) The horizontal lines indicate a median value, the spots indicate a mean value, the error lines indicate a standard deviation, and the boxes indicate the interquartile range. TEO, Thyme Essential Oil; PHMB, polyhexanide C+, growth control.

    Journal: Frontiers in Pharmacology

    Article Title: Toward essential oil stewardship: strain-resolved evaluation of thyme oil activity against Pseudomonas aeruginosa

    doi: 10.3389/fphar.2025.1659096

    Figure Lengend Snippet: Visual representation of the data distribution for antimicrobial and antibiofilm activity of the tested compounds against genetically distinct groups of P. aeruginosa strains. Distribution of growth inhibition zones values (mm). (A,B) Distribution of Minimal Inhibitory Concentration (MIC) (%, v/v). (C,D) Distribution of biofilm cells reduction (%). (E,F) The horizontal lines indicate a median value, the spots indicate a mean value, the error lines indicate a standard deviation, and the boxes indicate the interquartile range. TEO, Thyme Essential Oil; PHMB, polyhexanide C+, growth control.

    Article Snippet: The activity of TEO and PHMB against P. aeruginosa ATCC 15442 and P857 biofilms was confirmed also using a fluorescence microscope ( ).

    Techniques: Activity Assay, Inhibition, Concentration Assay, Standard Deviation, Control

    Comparison of antimicrobial and antibiofilm activity of the tested compounds against genetically distinct groups of P. aeruginosa strains. The mean diameters of growth inhibition zones (mm). (A) The mean values of Minimal Inhibitory Concentration (%, v/v) (MIC). (B) The mean reduction (log 10 ) of biofilm cells. (C) TEO, Thyme Essential Oil, PHMB, polyhexanide. The error lines represent a standard deviation.

    Journal: Frontiers in Pharmacology

    Article Title: Toward essential oil stewardship: strain-resolved evaluation of thyme oil activity against Pseudomonas aeruginosa

    doi: 10.3389/fphar.2025.1659096

    Figure Lengend Snippet: Comparison of antimicrobial and antibiofilm activity of the tested compounds against genetically distinct groups of P. aeruginosa strains. The mean diameters of growth inhibition zones (mm). (A) The mean values of Minimal Inhibitory Concentration (%, v/v) (MIC). (B) The mean reduction (log 10 ) of biofilm cells. (C) TEO, Thyme Essential Oil, PHMB, polyhexanide. The error lines represent a standard deviation.

    Article Snippet: The activity of TEO and PHMB against P. aeruginosa ATCC 15442 and P857 biofilms was confirmed also using a fluorescence microscope ( ).

    Techniques: Comparison, Activity Assay, Inhibition, Concentration Assay, Standard Deviation

    Summary of P. aeruginosa strains’ biofilm features and pseudomonal resistance to Thyme Essential Oil (TEO) or PHMB (polyhexanide) in genetically distinct groups. A heatmap was generated by setting a mean of all strains for each parameter ± 25% as high when the value was above the range (blue), low when the value was below the range (red) and medium when the value was equal to the range (grey). CFU/mL, Colony-Forming Unit/mL. MIC, Minimal Inhibitory Concentration.

    Journal: Frontiers in Pharmacology

    Article Title: Toward essential oil stewardship: strain-resolved evaluation of thyme oil activity against Pseudomonas aeruginosa

    doi: 10.3389/fphar.2025.1659096

    Figure Lengend Snippet: Summary of P. aeruginosa strains’ biofilm features and pseudomonal resistance to Thyme Essential Oil (TEO) or PHMB (polyhexanide) in genetically distinct groups. A heatmap was generated by setting a mean of all strains for each parameter ± 25% as high when the value was above the range (blue), low when the value was below the range (red) and medium when the value was equal to the range (grey). CFU/mL, Colony-Forming Unit/mL. MIC, Minimal Inhibitory Concentration.

    Article Snippet: The activity of TEO and PHMB against P. aeruginosa ATCC 15442 and P857 biofilms was confirmed also using a fluorescence microscope ( ).

    Techniques: Generated, Concentration Assay