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Antimicrobial activity of the tested compounds against P. aeruginosa strains assessed using a modified disk diffusion method. Results for diameters of growth inhibition zones (mm) (n = 10). (A) Representative zones of P857 strain growth inhibition after the treatment with Thyme Essential Oil (TEO). (B) Representative zones of P857 strain growth inhibition after the treatment with <t>polyhexanide</t> <t>(PHMB).</t> (C) The lines represent a mean value.
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Antimicrobial activity of the tested compounds against P. aeruginosa strains assessed using a modified disk diffusion method. Results for diameters of growth inhibition zones (mm) (n = 10). (A) Representative zones of P857 strain growth inhibition after the treatment with Thyme Essential Oil (TEO). (B) Representative zones of P857 strain growth inhibition after the treatment with <t>polyhexanide</t> <t>(PHMB).</t> (C) The lines represent a mean value.
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Antimicrobial activity of the tested compounds against P. aeruginosa strains assessed using a modified disk diffusion method. Results for diameters of growth inhibition zones (mm) (n = 10). (A) Representative zones of P857 strain growth inhibition after the treatment with Thyme Essential Oil (TEO). (B) Representative zones of P857 strain growth inhibition after the treatment with <t>polyhexanide</t> <t>(PHMB).</t> (C) The lines represent a mean value.
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Antimicrobial activity of the tested compounds against P. aeruginosa strains assessed using a modified disk diffusion method. Results for diameters of growth inhibition zones (mm) (n = 10). (A) Representative zones of P857 strain growth inhibition after the treatment with Thyme Essential Oil (TEO). (B) Representative zones of P857 strain growth inhibition after the treatment with <t>polyhexanide</t> <t>(PHMB).</t> (C) The lines represent a mean value.
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Antimicrobial activity of the tested compounds against P. aeruginosa strains assessed using a modified disk diffusion method. Results for diameters of growth inhibition zones (mm) (n = 10). (A) Representative zones of P857 strain growth inhibition after the treatment with Thyme Essential Oil (TEO). (B) Representative zones of P857 strain growth inhibition after the treatment with <t>polyhexanide</t> <t>(PHMB).</t> (C) The lines represent a mean value.
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Antimicrobial activity of the tested compounds against P. aeruginosa strains assessed using a modified disk diffusion method. Results for diameters of growth inhibition zones (mm) (n = 10). (A) Representative zones of P857 strain growth inhibition after the treatment with Thyme Essential Oil (TEO). (B) Representative zones of P857 strain growth inhibition after the treatment with <t>polyhexanide</t> <t>(PHMB).</t> (C) The lines represent a mean value.
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Antimicrobial activity of the tested compounds against P. aeruginosa strains assessed using a modified disk diffusion method. Results for diameters of growth inhibition zones (mm) (n = 10). (A) Representative zones of P857 strain growth inhibition after the treatment with Thyme Essential Oil (TEO). (B) Representative zones of P857 strain growth inhibition after the treatment with <t>polyhexanide</t> <t>(PHMB).</t> (C) The lines represent a mean value.
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Antimicrobial activity of the tested compounds against P. aeruginosa strains assessed using a modified disk diffusion method. Results for diameters of growth inhibition zones (mm) (n = 10). (A) Representative zones of P857 strain growth inhibition after the treatment with Thyme Essential Oil (TEO). (B) Representative zones of P857 strain growth inhibition after the treatment with <t>polyhexanide</t> <t>(PHMB).</t> (C) The lines represent a mean value.
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Antimicrobial activity of the tested compounds against P. aeruginosa strains assessed using a modified disk diffusion method. Results for diameters of growth inhibition zones (mm) (n = 10). (A) Representative zones of P857 strain growth inhibition after the treatment with Thyme Essential Oil (TEO). (B) Representative zones of P857 strain growth inhibition after the treatment with polyhexanide (PHMB). (C) The lines represent a mean value.

Journal: Frontiers in Pharmacology

Article Title: Toward essential oil stewardship: strain-resolved evaluation of thyme oil activity against Pseudomonas aeruginosa

doi: 10.3389/fphar.2025.1659096

Figure Lengend Snippet: Antimicrobial activity of the tested compounds against P. aeruginosa strains assessed using a modified disk diffusion method. Results for diameters of growth inhibition zones (mm) (n = 10). (A) Representative zones of P857 strain growth inhibition after the treatment with Thyme Essential Oil (TEO). (B) Representative zones of P857 strain growth inhibition after the treatment with polyhexanide (PHMB). (C) The lines represent a mean value.

Article Snippet: The activity of TEO and PHMB against P. aeruginosa ATCC 15442 and P857 biofilms was confirmed also using a fluorescence microscope ( ).

Techniques: Activity Assay, Modification, Diffusion-based Assay, Inhibition

Antibiofilm activity of the tested compounds against P. aeruginosa strains (n = 10) expressed as a reduction (log 10 ) of biofilm cells. TEO, Thyme Essential Oil, PHMB, polyhexanide. The lines represent a mean value.

Journal: Frontiers in Pharmacology

Article Title: Toward essential oil stewardship: strain-resolved evaluation of thyme oil activity against Pseudomonas aeruginosa

doi: 10.3389/fphar.2025.1659096

Figure Lengend Snippet: Antibiofilm activity of the tested compounds against P. aeruginosa strains (n = 10) expressed as a reduction (log 10 ) of biofilm cells. TEO, Thyme Essential Oil, PHMB, polyhexanide. The lines represent a mean value.

Article Snippet: The activity of TEO and PHMB against P. aeruginosa ATCC 15442 and P857 biofilms was confirmed also using a fluorescence microscope ( ).

Techniques: Activity Assay

Microscopic visualizations of the P. aeruginosa biofilm (covering the surface of a 24-well plate) stained with a LIVE/DEAD dye. ATCC 15442 strain’s untreated biofilm. (A) ATCC 15442 strain’s biofilm treated with Thyme Essential Oil. (B) ATCC 15442 strain’s biofilm treated with polyhexanide. (C) P857 strain’s untreated biofilm. (D) P857 strain’s biofilm treated with Thyme Essential Oil. (E) P857 strain’s biofilm treated with polyhexanide. (F) The red/orange color shows bacterial cells with altered/damaged cell walls; the green color indicates unaltered cell walls. Fluorescence microscope Etaluma 600 (magnification 4×). The well diameter was 15 mm.

Journal: Frontiers in Pharmacology

Article Title: Toward essential oil stewardship: strain-resolved evaluation of thyme oil activity against Pseudomonas aeruginosa

doi: 10.3389/fphar.2025.1659096

Figure Lengend Snippet: Microscopic visualizations of the P. aeruginosa biofilm (covering the surface of a 24-well plate) stained with a LIVE/DEAD dye. ATCC 15442 strain’s untreated biofilm. (A) ATCC 15442 strain’s biofilm treated with Thyme Essential Oil. (B) ATCC 15442 strain’s biofilm treated with polyhexanide. (C) P857 strain’s untreated biofilm. (D) P857 strain’s biofilm treated with Thyme Essential Oil. (E) P857 strain’s biofilm treated with polyhexanide. (F) The red/orange color shows bacterial cells with altered/damaged cell walls; the green color indicates unaltered cell walls. Fluorescence microscope Etaluma 600 (magnification 4×). The well diameter was 15 mm.

Article Snippet: The activity of TEO and PHMB against P. aeruginosa ATCC 15442 and P857 biofilms was confirmed also using a fluorescence microscope ( ).

Techniques: Staining, Fluorescence, Microscopy

Visual representation of the data distribution for antimicrobial and antibiofilm activity of the tested compounds against genetically distinct groups of P. aeruginosa strains. Distribution of growth inhibition zones values (mm). (A,B) Distribution of Minimal Inhibitory Concentration (MIC) (%, v/v). (C,D) Distribution of biofilm cells reduction (%). (E,F) The horizontal lines indicate a median value, the spots indicate a mean value, the error lines indicate a standard deviation, and the boxes indicate the interquartile range. TEO, Thyme Essential Oil; PHMB, polyhexanide C+, growth control.

Journal: Frontiers in Pharmacology

Article Title: Toward essential oil stewardship: strain-resolved evaluation of thyme oil activity against Pseudomonas aeruginosa

doi: 10.3389/fphar.2025.1659096

Figure Lengend Snippet: Visual representation of the data distribution for antimicrobial and antibiofilm activity of the tested compounds against genetically distinct groups of P. aeruginosa strains. Distribution of growth inhibition zones values (mm). (A,B) Distribution of Minimal Inhibitory Concentration (MIC) (%, v/v). (C,D) Distribution of biofilm cells reduction (%). (E,F) The horizontal lines indicate a median value, the spots indicate a mean value, the error lines indicate a standard deviation, and the boxes indicate the interquartile range. TEO, Thyme Essential Oil; PHMB, polyhexanide C+, growth control.

Article Snippet: The activity of TEO and PHMB against P. aeruginosa ATCC 15442 and P857 biofilms was confirmed also using a fluorescence microscope ( ).

Techniques: Activity Assay, Inhibition, Concentration Assay, Standard Deviation, Control

Comparison of antimicrobial and antibiofilm activity of the tested compounds against genetically distinct groups of P. aeruginosa strains. The mean diameters of growth inhibition zones (mm). (A) The mean values of Minimal Inhibitory Concentration (%, v/v) (MIC). (B) The mean reduction (log 10 ) of biofilm cells. (C) TEO, Thyme Essential Oil, PHMB, polyhexanide. The error lines represent a standard deviation.

Journal: Frontiers in Pharmacology

Article Title: Toward essential oil stewardship: strain-resolved evaluation of thyme oil activity against Pseudomonas aeruginosa

doi: 10.3389/fphar.2025.1659096

Figure Lengend Snippet: Comparison of antimicrobial and antibiofilm activity of the tested compounds against genetically distinct groups of P. aeruginosa strains. The mean diameters of growth inhibition zones (mm). (A) The mean values of Minimal Inhibitory Concentration (%, v/v) (MIC). (B) The mean reduction (log 10 ) of biofilm cells. (C) TEO, Thyme Essential Oil, PHMB, polyhexanide. The error lines represent a standard deviation.

Article Snippet: The activity of TEO and PHMB against P. aeruginosa ATCC 15442 and P857 biofilms was confirmed also using a fluorescence microscope ( ).

Techniques: Comparison, Activity Assay, Inhibition, Concentration Assay, Standard Deviation

Summary of P. aeruginosa strains’ biofilm features and pseudomonal resistance to Thyme Essential Oil (TEO) or PHMB (polyhexanide) in genetically distinct groups. A heatmap was generated by setting a mean of all strains for each parameter ± 25% as high when the value was above the range (blue), low when the value was below the range (red) and medium when the value was equal to the range (grey). CFU/mL, Colony-Forming Unit/mL. MIC, Minimal Inhibitory Concentration.

Journal: Frontiers in Pharmacology

Article Title: Toward essential oil stewardship: strain-resolved evaluation of thyme oil activity against Pseudomonas aeruginosa

doi: 10.3389/fphar.2025.1659096

Figure Lengend Snippet: Summary of P. aeruginosa strains’ biofilm features and pseudomonal resistance to Thyme Essential Oil (TEO) or PHMB (polyhexanide) in genetically distinct groups. A heatmap was generated by setting a mean of all strains for each parameter ± 25% as high when the value was above the range (blue), low when the value was below the range (red) and medium when the value was equal to the range (grey). CFU/mL, Colony-Forming Unit/mL. MIC, Minimal Inhibitory Concentration.

Article Snippet: The activity of TEO and PHMB against P. aeruginosa ATCC 15442 and P857 biofilms was confirmed also using a fluorescence microscope ( ).

Techniques: Generated, Concentration Assay