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dnmt1-luc, containing the sequence from −25 to +232 of the human dnmt1 promoter in pgl2-basic vector  (Promega)

 
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    Structured Review

    Promega dnmt1-luc, containing the sequence from −25 to +232 of the human dnmt1 promoter in pgl2-basic vector
    Dnmt1 Luc, Containing The Sequence From −25 To +232 Of The Human Dnmt1 Promoter In Pgl2 Basic Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl2-basic-luc/pgl2+based+ucp2+firefly+luciferase+constructs/pm27317649-46-1-17
    Average 90 stars, based on 1 article reviews
    dnmt1-luc, containing the sequence from −25 to +232 of the human dnmt1 promoter in pgl2-basic vector - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: IL-6 stimulates annexin 1 expression and translocation and suggests a new biological role as class II acute phase protein.
    Article Snippet: Annexin 1 (Ax 1), a protein whose synthesis and secretion are induced during the inflammatory response, has been proposed as a mediator of the anti-inflammatory action of glucocorticoids.. To gain insight into a broader role of Ax 1 during the inflammatory response, the authors have investigated how pro-inflammatory cytokines [interleukin 1 (IL-1), IL-6 and tumour necrosis factor a (TNF-a)] affect Ax 1 expression and regulation at transcriptional and translational levels.. The authors show that induction of the Ax 1 protein and its translocation to the cell membrane are stimulated by interleukin 6.

    Article Title: Carnitine palmitoyltransferase 1A functions to repress FoxO transcription factors to allow cell cycle progression in ovarian cancer
    Article Snippet: The p21 gene promoter in WWP-Luc was obtained from Dr. B Vogelstein [ ] and re-cloned into pGL2-Basic-Luc (Promega, Madison, WI) to make pGL2-p21-Luc.

    Article Title: Dexamethasone and tumor necrosis factor-alpha act together to induce the cellular inhibitor of apoptosis-2 gene and prevent apoptosis in a variety of cell types.
    Article Snippet: The resulting PCR products were cloned into pcDNA2.1 Topo (Invitrogen, Carlsbad, CA), sequence confirmed, and subcloned into pGL2-basic-LUC (Promega Corp., Madison, WI) at the XhoI and HindIII sites, yielding IAP( 947– 54)-LUC and IAP( 395– 54)-LUC, respectively (see Fig. 1).

    Clone Assay:

    Article Title: IL-6 stimulates annexin 1 expression and translocation and suggests a new biological role as class II acute phase protein.
    Article Snippet: Annexin 1 (Ax 1), a protein whose synthesis and secretion are induced during the inflammatory response, has been proposed as a mediator of the anti-inflammatory action of glucocorticoids.. To gain insight into a broader role of Ax 1 during the inflammatory response, the authors have investigated how pro-inflammatory cytokines [interleukin 1 (IL-1), IL-6 and tumour necrosis factor a (TNF-a)] affect Ax 1 expression and regulation at transcriptional and translational levels.. The authors show that induction of the Ax 1 protein and its translocation to the cell membrane are stimulated by interleukin 6.

    Article Title: Carnitine palmitoyltransferase 1A functions to repress FoxO transcription factors to allow cell cycle progression in ovarian cancer
    Article Snippet: The p21 gene promoter in WWP-Luc was obtained from Dr. B Vogelstein [ ] and re-cloned into pGL2-Basic-Luc (Promega, Madison, WI) to make pGL2-p21-Luc.

    Article Title: Dexamethasone and tumor necrosis factor-alpha act together to induce the cellular inhibitor of apoptosis-2 gene and prevent apoptosis in a variety of cell types.
    Article Snippet: The resulting PCR products were cloned into pcDNA2.1 Topo (Invitrogen, Carlsbad, CA), sequence confirmed, and subcloned into pGL2-basic-LUC (Promega Corp., Madison, WI) at the XhoI and HindIII sites, yielding IAP( 947– 54)-LUC and IAP( 395– 54)-LUC, respectively (see Fig. 1).

    Sequencing:

    Article Title: IL-6 stimulates annexin 1 expression and translocation and suggests a new biological role as class II acute phase protein.
    Article Snippet: Annexin 1 (Ax 1), a protein whose synthesis and secretion are induced during the inflammatory response, has been proposed as a mediator of the anti-inflammatory action of glucocorticoids.. To gain insight into a broader role of Ax 1 during the inflammatory response, the authors have investigated how pro-inflammatory cytokines [interleukin 1 (IL-1), IL-6 and tumour necrosis factor a (TNF-a)] affect Ax 1 expression and regulation at transcriptional and translational levels.. The authors show that induction of the Ax 1 protein and its translocation to the cell membrane are stimulated by interleukin 6.

    Article Title: Carnitine palmitoyltransferase 1A functions to repress FoxO transcription factors to allow cell cycle progression in ovarian cancer
    Article Snippet: The p21 gene promoter in WWP-Luc was obtained from Dr. B Vogelstein [ ] and re-cloned into pGL2-Basic-Luc (Promega, Madison, WI) to make pGL2-p21-Luc.

    Article Title: Dexamethasone and tumor necrosis factor-alpha act together to induce the cellular inhibitor of apoptosis-2 gene and prevent apoptosis in a variety of cell types.
    Article Snippet: The resulting PCR products were cloned into pcDNA2.1 Topo (Invitrogen, Carlsbad, CA), sequence confirmed, and subcloned into pGL2-basic-LUC (Promega Corp., Madison, WI) at the XhoI and HindIII sites, yielding IAP( 947– 54)-LUC and IAP( 395– 54)-LUC, respectively (see Fig. 1).

    Plasmid Preparation:

    Article Title: IL-6 stimulates annexin 1 expression and translocation and suggests a new biological role as class II acute phase protein.
    Article Snippet: Annexin 1 (Ax 1), a protein whose synthesis and secretion are induced during the inflammatory response, has been proposed as a mediator of the anti-inflammatory action of glucocorticoids.. To gain insight into a broader role of Ax 1 during the inflammatory response, the authors have investigated how pro-inflammatory cytokines [interleukin 1 (IL-1), IL-6 and tumour necrosis factor a (TNF-a)] affect Ax 1 expression and regulation at transcriptional and translational levels.. The authors show that induction of the Ax 1 protein and its translocation to the cell membrane are stimulated by interleukin 6.

    Article Title: Carnitine palmitoyltransferase 1A functions to repress FoxO transcription factors to allow cell cycle progression in ovarian cancer
    Article Snippet: The p21 gene promoter in WWP-Luc was obtained from Dr. B Vogelstein [ ] and re-cloned into pGL2-Basic-Luc (Promega, Madison, WI) to make pGL2-p21-Luc.

    Article Title: Dexamethasone and tumor necrosis factor-alpha act together to induce the cellular inhibitor of apoptosis-2 gene and prevent apoptosis in a variety of cell types.
    Article Snippet: The resulting PCR products were cloned into pcDNA2.1 Topo (Invitrogen, Carlsbad, CA), sequence confirmed, and subcloned into pGL2-basic-LUC (Promega Corp., Madison, WI) at the XhoI and HindIII sites, yielding IAP( 947– 54)-LUC and IAP( 395– 54)-LUC, respectively (see Fig. 1).

    Luciferase:

    Article Title: IL-6 stimulates annexin 1 expression and translocation and suggests a new biological role as class II acute phase protein.
    Article Snippet: Annexin 1 (Ax 1), a protein whose synthesis and secretion are induced during the inflammatory response, has been proposed as a mediator of the anti-inflammatory action of glucocorticoids.. To gain insight into a broader role of Ax 1 during the inflammatory response, the authors have investigated how pro-inflammatory cytokines [interleukin 1 (IL-1), IL-6 and tumour necrosis factor a (TNF-a)] affect Ax 1 expression and regulation at transcriptional and translational levels.. The authors show that induction of the Ax 1 protein and its translocation to the cell membrane are stimulated by interleukin 6.

    Article Title: Carnitine palmitoyltransferase 1A functions to repress FoxO transcription factors to allow cell cycle progression in ovarian cancer
    Article Snippet: The p21 gene promoter in WWP-Luc was obtained from Dr. B Vogelstein [ ] and re-cloned into pGL2-Basic-Luc (Promega, Madison, WI) to make pGL2-p21-Luc.

    Article Title: Dexamethasone and tumor necrosis factor-alpha act together to induce the cellular inhibitor of apoptosis-2 gene and prevent apoptosis in a variety of cell types.
    Article Snippet: The resulting PCR products were cloned into pcDNA2.1 Topo (Invitrogen, Carlsbad, CA), sequence confirmed, and subcloned into pGL2-basic-LUC (Promega Corp., Madison, WI) at the XhoI and HindIII sites, yielding IAP( 947– 54)-LUC and IAP( 395– 54)-LUC, respectively (see Fig. 1).



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    Image Search Results


    Stamp2 promoter is activated by TNFα and p50. (A) 3T3-L1 cells with stable expression of either the −2kb-ST2-LUC or a BASIC-LUC reporters were differentiated into adipocytes, treated with TNFα (10 ng/ml) or vehicle (Ctrl) for 24 h and subjected to qRT-PCR analysis and (B) LUC assay. Values are presented as fold activity compared to vehicle. Results are from two independent experiments, n=9 for each sample. (C) HeLa cells were co-transfected with either 2xNFκB-LUC or −2kb-ST2-LUC (ST2-LUC), together with either AllStars (siAll) or NFKB1 (siNFKB1) siRNA. After 48 h, cells were harvested and LUC activity was determined. Values are presented as fold activity compared to control (siALL) from two independent experiments, n=6 for each sample. (D) qRT-PCR analysis of NFKB1 (p50) mRNA from the same cells used in C. Values are presented as fold activity compared to control (siALL). Results are from two independent experiments, n=6 for each sample. (E) HeLa cells were co-transfected with either 2xNFκB-LUC or −2kb-ST2-LUC (ST2-LUC), together with either a p50 expression plasmid or an empty control plasmid. After 48 h, cells were harvested and LUC activity was determined. Values are presented as fold activity compared to control (Ctrl) from two independent experiments, n=6 for each sample.

    Journal: Metabolism: clinical and experimental

    Article Title: Inflammation and ER stress differentially regulate STAMP2 expression and localization in adipocytes

    doi: 10.1016/j.metabol.2019.01.014

    Figure Lengend Snippet: Stamp2 promoter is activated by TNFα and p50. (A) 3T3-L1 cells with stable expression of either the −2kb-ST2-LUC or a BASIC-LUC reporters were differentiated into adipocytes, treated with TNFα (10 ng/ml) or vehicle (Ctrl) for 24 h and subjected to qRT-PCR analysis and (B) LUC assay. Values are presented as fold activity compared to vehicle. Results are from two independent experiments, n=9 for each sample. (C) HeLa cells were co-transfected with either 2xNFκB-LUC or −2kb-ST2-LUC (ST2-LUC), together with either AllStars (siAll) or NFKB1 (siNFKB1) siRNA. After 48 h, cells were harvested and LUC activity was determined. Values are presented as fold activity compared to control (siALL) from two independent experiments, n=6 for each sample. (D) qRT-PCR analysis of NFKB1 (p50) mRNA from the same cells used in C. Values are presented as fold activity compared to control (siALL). Results are from two independent experiments, n=6 for each sample. (E) HeLa cells were co-transfected with either 2xNFκB-LUC or −2kb-ST2-LUC (ST2-LUC), together with either a p50 expression plasmid or an empty control plasmid. After 48 h, cells were harvested and LUC activity was determined. Values are presented as fold activity compared to control (Ctrl) from two independent experiments, n=6 for each sample.

    Article Snippet: To make stable reporter cells, linearized pGL2-BASIC-LUC or pGL2-BASIC-2kb-ST2-LUC was co-transfected with the linearized selection vector pPur (Clontech) in a 5:1 ratio using Lipofectamine 2000 (Invitrogen) as transfection agent.

    Techniques: Expressing, Quantitative RT-PCR, Activity Assay, Transfection, Plasmid Preparation

    Stamp2 promoter activity requires C/EBPα. (A) 3T3-L1 cells stably expressing the −2kb-ST2-LUC reporter were differentiated into adipocytes. They were then left untreated or incubated with Tg (300 nM), or Tu (2 ng/ml) for 24 h. Cells were then harvested and LUC activity was determined. Results are from two independent experiments, n=8 for each sample. (B) 3T3-L1 cells with stable expression of the −2kb-ST2-LUC reporter were transfected with either AllStar (siAll) or Cepba (siCEBPA) targeted siRNA, and differentiated towards adipocytes for 4 days. Cells were then harvested and LUC activities were determined. Values are presented as fold activity compared to control (siALL) from two independent experiments, n=6 for each sample. (C) qRT-PCR analysis of CEBPA mRNA from the same cells used in (B). (D) Overview of two predicted C/EBPα binding motifs (C/EBP1 and 2) in the mouse Stamp2 promoter. The nucleotides that are mutated are underlined. (E) HeLa cells were co-transfected with an expression vector for C/EBPα and either BASIC-LUC reporter plasmid (Ctrl), the wild-type −2kb-ST2-LUC, or a −2kb-ST2-LUC reporter construct mutated in C/EBP1 or C/EBP2 binding sites (MUT1 and MUT2, respectively). After 24 h, cells were harvested and LUC activities were determined. The data shown are from three independent experiments, n=9 for each sample.

    Journal: Metabolism: clinical and experimental

    Article Title: Inflammation and ER stress differentially regulate STAMP2 expression and localization in adipocytes

    doi: 10.1016/j.metabol.2019.01.014

    Figure Lengend Snippet: Stamp2 promoter activity requires C/EBPα. (A) 3T3-L1 cells stably expressing the −2kb-ST2-LUC reporter were differentiated into adipocytes. They were then left untreated or incubated with Tg (300 nM), or Tu (2 ng/ml) for 24 h. Cells were then harvested and LUC activity was determined. Results are from two independent experiments, n=8 for each sample. (B) 3T3-L1 cells with stable expression of the −2kb-ST2-LUC reporter were transfected with either AllStar (siAll) or Cepba (siCEBPA) targeted siRNA, and differentiated towards adipocytes for 4 days. Cells were then harvested and LUC activities were determined. Values are presented as fold activity compared to control (siALL) from two independent experiments, n=6 for each sample. (C) qRT-PCR analysis of CEBPA mRNA from the same cells used in (B). (D) Overview of two predicted C/EBPα binding motifs (C/EBP1 and 2) in the mouse Stamp2 promoter. The nucleotides that are mutated are underlined. (E) HeLa cells were co-transfected with an expression vector for C/EBPα and either BASIC-LUC reporter plasmid (Ctrl), the wild-type −2kb-ST2-LUC, or a −2kb-ST2-LUC reporter construct mutated in C/EBP1 or C/EBP2 binding sites (MUT1 and MUT2, respectively). After 24 h, cells were harvested and LUC activities were determined. The data shown are from three independent experiments, n=9 for each sample.

    Article Snippet: To make stable reporter cells, linearized pGL2-BASIC-LUC or pGL2-BASIC-2kb-ST2-LUC was co-transfected with the linearized selection vector pPur (Clontech) in a 5:1 ratio using Lipofectamine 2000 (Invitrogen) as transfection agent.

    Techniques: Activity Assay, Stable Transfection, Expressing, Incubation, Transfection, Quantitative RT-PCR, Binding Assay, Plasmid Preparation, Construct

    Stamp2 promoter is activated by TNFα and p50. (A) 3T3-L1 cells with stable expression of either the −2kb-ST2-LUC or a BASIC-LUC reporters were differentiated into adipocytes, treated with TNFα (10 ng/ml) or vehicle (Ctrl) for 24 h and subjected to qRT-PCR analysis and (B) LUC assay. Values are presented as fold activity compared to vehicle. Results are from two independent experiments, n=9 for each sample. (C) HeLa cells were co-transfected with either 2xNFκB-LUC or −2kb-ST2-LUC (ST2-LUC), together with either AllStars (siAll) or NFKB1 (siNFKB1) siRNA. After 48 h, cells were harvested and LUC activity was determined. Values are presented as fold activity compared to control (siALL) from two independent experiments, n=6 for each sample. (D) qRT-PCR analysis of NFKB1 (p50) mRNA from the same cells used in C. Values are presented as fold activity compared to control (siALL). Results are from two independent experiments, n=6 for each sample. (E) HeLa cells were co-transfected with either 2xNFκB-LUC or −2kb-ST2-LUC (ST2-LUC), together with either a p50 expression plasmid or an empty control plasmid. After 48 h, cells were harvested and LUC activity was determined. Values are presented as fold activity compared to control (Ctrl) from two independent experiments, n=6 for each sample.

    Journal: Metabolism: clinical and experimental

    Article Title: Inflammation and ER stress differentially regulate STAMP2 expression and localization in adipocytes

    doi: 10.1016/j.metabol.2019.01.014

    Figure Lengend Snippet: Stamp2 promoter is activated by TNFα and p50. (A) 3T3-L1 cells with stable expression of either the −2kb-ST2-LUC or a BASIC-LUC reporters were differentiated into adipocytes, treated with TNFα (10 ng/ml) or vehicle (Ctrl) for 24 h and subjected to qRT-PCR analysis and (B) LUC assay. Values are presented as fold activity compared to vehicle. Results are from two independent experiments, n=9 for each sample. (C) HeLa cells were co-transfected with either 2xNFκB-LUC or −2kb-ST2-LUC (ST2-LUC), together with either AllStars (siAll) or NFKB1 (siNFKB1) siRNA. After 48 h, cells were harvested and LUC activity was determined. Values are presented as fold activity compared to control (siALL) from two independent experiments, n=6 for each sample. (D) qRT-PCR analysis of NFKB1 (p50) mRNA from the same cells used in C. Values are presented as fold activity compared to control (siALL). Results are from two independent experiments, n=6 for each sample. (E) HeLa cells were co-transfected with either 2xNFκB-LUC or −2kb-ST2-LUC (ST2-LUC), together with either a p50 expression plasmid or an empty control plasmid. After 48 h, cells were harvested and LUC activity was determined. Values are presented as fold activity compared to control (Ctrl) from two independent experiments, n=6 for each sample.

    Article Snippet: To make stable reporter cells, linearized pGL2-BASIC-LUC or pGL2-BASIC-2kb-ST2-LUC was co-transfected with the linearized selection vector pPur (Clontech) in a 5:1 ratio using Lipofectamine 2000 (Invitrogen) as transfection agent.

    Techniques: Expressing, Quantitative RT-PCR, Activity Assay, Transfection, Plasmid Preparation

    Stamp2 promoter activity requires C/EBPα. (A) 3T3-L1 cells stably expressing the −2kb-ST2-LUC reporter were differentiated into adipocytes. They were then left untreated or incubated with Tg (300 nM), or Tu (2 ng/ml) for 24 h. Cells were then harvested and LUC activity was determined. Results are from two independent experiments, n=8 for each sample. (B) 3T3-L1 cells with stable expression of the −2kb-ST2-LUC reporter were transfected with either AllStar (siAll) or Cepba (siCEBPA) targeted siRNA, and differentiated towards adipocytes for 4 days. Cells were then harvested and LUC activities were determined. Values are presented as fold activity compared to control (siALL) from two independent experiments, n=6 for each sample. (C) qRT-PCR analysis of CEBPA mRNA from the same cells used in (B). (D) Overview of two predicted C/EBPα binding motifs (C/EBP1 and 2) in the mouse Stamp2 promoter. The nucleotides that are mutated are underlined. (E) HeLa cells were co-transfected with an expression vector for C/EBPα and either BASIC-LUC reporter plasmid (Ctrl), the wild-type −2kb-ST2-LUC, or a −2kb-ST2-LUC reporter construct mutated in C/EBP1 or C/EBP2 binding sites (MUT1 and MUT2, respectively). After 24 h, cells were harvested and LUC activities were determined. The data shown are from three independent experiments, n=9 for each sample.

    Journal: Metabolism: clinical and experimental

    Article Title: Inflammation and ER stress differentially regulate STAMP2 expression and localization in adipocytes

    doi: 10.1016/j.metabol.2019.01.014

    Figure Lengend Snippet: Stamp2 promoter activity requires C/EBPα. (A) 3T3-L1 cells stably expressing the −2kb-ST2-LUC reporter were differentiated into adipocytes. They were then left untreated or incubated with Tg (300 nM), or Tu (2 ng/ml) for 24 h. Cells were then harvested and LUC activity was determined. Results are from two independent experiments, n=8 for each sample. (B) 3T3-L1 cells with stable expression of the −2kb-ST2-LUC reporter were transfected with either AllStar (siAll) or Cepba (siCEBPA) targeted siRNA, and differentiated towards adipocytes for 4 days. Cells were then harvested and LUC activities were determined. Values are presented as fold activity compared to control (siALL) from two independent experiments, n=6 for each sample. (C) qRT-PCR analysis of CEBPA mRNA from the same cells used in (B). (D) Overview of two predicted C/EBPα binding motifs (C/EBP1 and 2) in the mouse Stamp2 promoter. The nucleotides that are mutated are underlined. (E) HeLa cells were co-transfected with an expression vector for C/EBPα and either BASIC-LUC reporter plasmid (Ctrl), the wild-type −2kb-ST2-LUC, or a −2kb-ST2-LUC reporter construct mutated in C/EBP1 or C/EBP2 binding sites (MUT1 and MUT2, respectively). After 24 h, cells were harvested and LUC activities were determined. The data shown are from three independent experiments, n=9 for each sample.

    Article Snippet: To make stable reporter cells, linearized pGL2-BASIC-LUC or pGL2-BASIC-2kb-ST2-LUC was co-transfected with the linearized selection vector pPur (Clontech) in a 5:1 ratio using Lipofectamine 2000 (Invitrogen) as transfection agent.

    Techniques: Activity Assay, Stable Transfection, Expressing, Incubation, Transfection, Quantitative RT-PCR, Binding Assay, Plasmid Preparation, Construct