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cxcl9  (MedChemExpress)


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    MedChemExpress cxcl9
    Cxcl9, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p70008/pm41614892-84-5-14?v=MedChemExpress
    Average 94 stars, based on 6 article reviews
    cxcl9 - by Bioz Stars, 2026-08
    94/100 stars

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    M1-Mφ-derived CXCL9 impairs trophoblasts invasion and migration. (A) mRNA expression levels in HTR-8 cells cultured alone or co-cultured with M1-Mφ. (B) ELISA assays of CXCL9 in the supernatant of M1-Mφ alone or co-cultured with HTR-8 cells. (C) CXCL9 mRNA expression in HTR-8 and M1-Mφ with or without 48 h of co-culture. (D-F) The expression of EMT markers in HTR-8 cells alone, CXCL9-supplemented HTR-8 cells, M1-Mφ-co-cultured HTR-8 cells, <t>and</t> <t>anti-CXCL9</t> M1-Mφ-co-cultured HTR-8 cells were analyzed by qPCR, western blot and immunofluorescence. Scale bar: 20 μm. (G, H) Migration and invasion of anti-CXCL9 M1-Mφ-co-cultured HTR-8 cells and its control were measured by wound-healing assay and transwell assays, respectively. n = 3, Scale bar: 50 μm; * P < 0.05, ** P < 0.01, *** P < 0.001.
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    M1-Mφ-derived CXCL9 impairs trophoblasts invasion and migration. (A) mRNA expression levels in HTR-8 cells cultured alone or co-cultured with M1-Mφ. (B) ELISA assays of CXCL9 in the supernatant of M1-Mφ alone or co-cultured with HTR-8 cells. (C) CXCL9 mRNA expression in HTR-8 and M1-Mφ with or without 48 h of co-culture. (D-F) The expression of EMT markers in HTR-8 cells alone, CXCL9-supplemented HTR-8 cells, M1-Mφ-co-cultured HTR-8 cells, and anti-CXCL9 M1-Mφ-co-cultured HTR-8 cells were analyzed by qPCR, western blot and immunofluorescence. Scale bar: 20 μm. (G, H) Migration and invasion of anti-CXCL9 M1-Mφ-co-cultured HTR-8 cells and its control were measured by wound-healing assay and transwell assays, respectively. n = 3, Scale bar: 50 μm; * P < 0.05, ** P < 0.01, *** P < 0.001.

    Journal: Frontiers in Immunology

    Article Title: Reciprocal activation between M1 macrophages and trophoblasts through CXCL9/STAT1/ZEB1/CCL2 axis promotes recurrent spontaneous abortion

    doi: 10.3389/fimmu.2025.1629370

    Figure Lengend Snippet: M1-Mφ-derived CXCL9 impairs trophoblasts invasion and migration. (A) mRNA expression levels in HTR-8 cells cultured alone or co-cultured with M1-Mφ. (B) ELISA assays of CXCL9 in the supernatant of M1-Mφ alone or co-cultured with HTR-8 cells. (C) CXCL9 mRNA expression in HTR-8 and M1-Mφ with or without 48 h of co-culture. (D-F) The expression of EMT markers in HTR-8 cells alone, CXCL9-supplemented HTR-8 cells, M1-Mφ-co-cultured HTR-8 cells, and anti-CXCL9 M1-Mφ-co-cultured HTR-8 cells were analyzed by qPCR, western blot and immunofluorescence. Scale bar: 20 μm. (G, H) Migration and invasion of anti-CXCL9 M1-Mφ-co-cultured HTR-8 cells and its control were measured by wound-healing assay and transwell assays, respectively. n = 3, Scale bar: 50 μm; * P < 0.05, ** P < 0.01, *** P < 0.001.

    Article Snippet: In the anti-CXCL9 group, anti-Mouse CXCL9/MIG Antibody (1 mg/kg; MCE, Shanghai) were injected into female C57BL/6 mice by intravenously administered at 8:00 am on E7.5, E10.5 and E13.5.

    Techniques: Derivative Assay, Migration, Expressing, Cell Culture, Enzyme-linked Immunosorbent Assay, Co-Culture Assay, Western Blot, Immunofluorescence, Control, Wound Healing Assay

    Anti-CXCL9 treatment alleviates embryo resorption rate in mice. (A) Experimental protocol for LPS-induced abortion model with anti-CXCL9 treatment. (B) The embryo resorption rates in the control, LPS-induced abortion and anti-CXCL9 groups. (C, D) Placental interface expression of E-cadherin and Vimentin. (E) IHC analysis of CD86 at the placental interface of mice. (F-H) IHC of p-STAT1, IRF1 and ZEB1 at the placental interface of mice. Scale bar, 50 µm. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Journal: Frontiers in Immunology

    Article Title: Reciprocal activation between M1 macrophages and trophoblasts through CXCL9/STAT1/ZEB1/CCL2 axis promotes recurrent spontaneous abortion

    doi: 10.3389/fimmu.2025.1629370

    Figure Lengend Snippet: Anti-CXCL9 treatment alleviates embryo resorption rate in mice. (A) Experimental protocol for LPS-induced abortion model with anti-CXCL9 treatment. (B) The embryo resorption rates in the control, LPS-induced abortion and anti-CXCL9 groups. (C, D) Placental interface expression of E-cadherin and Vimentin. (E) IHC analysis of CD86 at the placental interface of mice. (F-H) IHC of p-STAT1, IRF1 and ZEB1 at the placental interface of mice. Scale bar, 50 µm. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Article Snippet: In the anti-CXCL9 group, anti-Mouse CXCL9/MIG Antibody (1 mg/kg; MCE, Shanghai) were injected into female C57BL/6 mice by intravenously administered at 8:00 am on E7.5, E10.5 and E13.5.

    Techniques: Control, Expressing, Paraffin-embedded Immunohistochemistry