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KEY RESOURCES TABLE
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Image Search Results


KEY RESOURCES TABLE

Journal: Molecular cell

Article Title: Rad52 Restrains Resection at DNA Double-Strand Break Ends in Yeast

doi: 10.1016/j.molcel.2019.08.017

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Images were captured by the Azure c400 Imaging System (Azure Biosystems). table ft1 table-wrap mode="anchored" t5 caption a7 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal ANTI-FLAG M2-Peroxidase (HRP) antibody produced in mouse Sigma-Aldrich A8592–1MG; RRID:AB_439702 Monoclonal Anti-HA antibody produced in mouse Sigma-Aldrich H3663–100UL; RRID:AB_262051 Goat Anti-Mouse Goat anti-mouse IgG-HRP Polyclonal, Hrp Conjugated antibody Santa Cruz Biotechnology sc-2005; RRID:AB_631736 PGK1 antibody [22C5D8] abcam ab113687; RRID:AB_10861977 Monoclonal ANTI-FLAG M2 antibody produced in mouse Sigma-Aldrich F3165–1MG; RRID:AB_259529 Monoclonal Anti-c-Myc antibody produced in mouse Sigma-Aldrich M4439–100UL; RRID:AB_439694 Chemicals, Peptides, and Recombinant Proteins Anhydrotetracycline hydrochloride (ahTET) Acros Organics AC23313–1000 cOmplete, Mini Protease Inhibitor Cocktail Roche 4693124001 Protein G-Agarose Roche 11243233001 Ribonuclease A from bovine pancreas Sigma-Aldrich R6513–250MG Easytides dATP, [α- 32 P] PerkinElmer BLU512H250UC Hydroxyurea (HU) Sigma-Aldrich H8627–100G Camptothecin (CPT) Sigma-Aldrich C9911–250MG Phleomycin InvivoGen ant-ph-5 Methyl methanesulfonate (MMS) Sigma-Aldrich M4016–1G Doxycycline hyclate Sigma-Aldrich D9891–5G SNAP-Surface Alexa Fluor 546 NEB S9129S Chitin resin NEB S6651L cOmplete His-tag resin Roche 5893682001 SP Sepharose GE Healthcare 28950513 anti-FLAG-M2 resin Sigma A1205 PD10 desalting column GE Healthcare 17085101 DOPC Avanti Polar Lipids 850375C biotinylated-PE Avanti Polar Lipids 860562C mPEG 2OOO-DOPE Avanti Polar Lipids 880130C quartz slides Finkenbeiner N/A Polymethylmethacrylate 25 kDa Polymer Source P9790-MMA 3% PMMA 495 kDa MICROCHEM N/A AquaSave Mitsubishi Rayon Co., Ltd N/A λ-dsDNA NEB N3011S M13mp18 ssDNA NEB N4040S TLC plates Millipore HX71732079 Critical Commercial Assays amfiSure ONE PCR Master Mix (2×) GenDEPOT P7000–010 In-Fusion HD Cloning Plus TaKaRa Bio 638909 Ex Taq DNA Polymerase TaKaRa Bio RR001A NucleoSpin Gel and PCR clean-up MACHEREY-NAGEL 740609.250 RadPrime DNA Labeling System Invitrogen 18428–011 PowerUp SYBR Green Master Mix Applied Biosystems A25742 Amersham ECL Prime Western Blotting Detection Reagent GE Healthcare RPN2232 Deposited Data Unprocessed autoradiographs, western blot images and fluorescence microscopy images of this study This study https://doi.org/10.17632/g566xfgd96.1 Experimental Models: Organisms/Strains Fission and budding yeast strains used in this study are listed in Table S1 and Table S2 .

Techniques: Produced, Recombinant, Protease Inhibitor, Clone Assay, DNA Labeling, SYBR Green Assay, Western Blot, Fluorescence, Microscopy, Software, Real-time Polymerase Chain Reaction, Imaging, Hybridization