e g7  (ATCC)

Journal: Bioactive Materials

Article Title: Splenic dendritic cell-targeting mRNA transfection of H-type ionizable lipid-based LNPs for enhancing tumor immunotherapy

doi: 10.1016/j.bioactmat.2026.02.018

Figure Lengend Snippet: Anti-tumor effects of mOVA/H 18 NPs as a preventive tumor vaccine. (A) Schematic illustration of experiment design. C57BL/6J mice were vaccinated twice on Day −14 and Day −7 through intravenous injection. On Day 0, B16-OVA cells were inoculated subcutaneously on C57BL/6J mice. (B) Tumor growth curves and (C) survival rate of B16-OVA-bearing mice (n = 6). The survival rates of the two groups were analyzed using a log-rank test. Individual tumor growth curves of mice in (D) PBS group, (E) OVA protein group, (F) mOVA/MC3-LNPs group, and (G) mOVA/H 18 NPs group (n = 6). Representative flow cytometry contour plots (H) and quantification (I) of the percentage of T EM in CD8 + T cells in the spleen (n = 3).

Article Snippet: OVA protein was purchased from Shanghai Yuanye Bio-Technology Co., Ltd (Shanghai, China).

Techniques: Injection, Flow Cytometry

Journal: STAR Protocols

Article Title: Protocol for an in vivo CRISPR screen for germinal center B cells in mice using ecotropic retrovirus

doi: 10.1016/j.xpro.2026.104586

Figure Lengend Snippet: Immunization experiment scheme for in vivo screen Host mice (IOMA gl) were immunized with OVA in alum. At week 2 after immunization, B cells from B1-8 hi , LSL-Cas9, AID-Cre, Kappa KO animals were transduced with pooled sgRNA library retrovirus and adoptively transferred to the hosts. Subsequently, the host animals were boosted with NP-OVA in alum at day 0 and 2 and administered with anti-DEC205-OVA mAb at day 6.5. Spleens were harvested and processed for cell sorting at day 10.

Article Snippet: NP-OVA (conjugation ratio: 21) , Biosearch Technologies , cat# N-5051.

Techniques: In Vivo, Transduction, FACS

Journal: Science Advances

Article Title: HSPCs and T reg cells cooperate to preserve extramedullary hematopoiesis under chronic inflammation

doi: 10.1126/sciadv.adv9351

Figure Lengend Snippet: In this figure, CD53 + and CD53 − HSPCs were sorted on the basis of CD53 surface expression. ( A ) Representative histogram plot of splenic HSCs from CMO mice. The x axis indicates MHCII levels in CD53 − and CD53 + HSCs. Gray peak indicates signal for fluorescence minus one (FMO). ( B ) Frequency of MHCII + in CD53 − and CD53 + MPP and HSC from CMO BM, spleen, and paw. The y axes indicate percentage of MHCII + cells. ( C ) Schematic representation of coculture experiments. ( D ) Representative histogram plot of T cell proliferation after 4 days in coculture with DCs (T DC ; green), CD53 + HSPCs (T CD53+ ; blue), or CD53 − HSPCs (T CD53− ; gray) in the presence of ovalbumin 247-264 peptide (OVA). The negative control (dashed line) represents naïve CD4 + OTII T cells and OVA without APCs. The x axis indicates levels of Cell proliferation dye (CPD). ( E ) Percentage of proliferated CD4 + OTII T cells after 4 days of coculture with CD53 − HSPCs (gray), CD53 + HSPCs (blue), and DCs (green) in the presence of OVA. ( F ) Percentage of anergic CD4 + OTII T cells after 4 days of coculture with CD53 − HSPCs (gray), CD53 + HSPCs (blue), and DCs (green) in the presence of OVA. ( G ) Frequency of viable CD4 + OTII T cells after 4 days of coculture with indicated cells in the presence of OVA. The y axis indicates percentage of viable CD4 + OTII cells. ( H ) Number of OTII T reg cells after 4 days of coculture with the distinct cells types and in the presence of OVA. ( I ) Frequency of c-Kit + HSPCs after 4 days of coculture with naïve CD4 + OTII cells. First two columns represent negative control cocultures containing HSPCs and naïve CD4 + OTII cells without OVA. The other columns represent cocultures containing HSPCs, naïve CD4 + OTII cells, and OVA. HSPCs were CD53 − (gray) or CD53 + (blue). Mice used were 16- to 25-week-old male and female mice. * P < 0.05; ** P < 0.01; *** P < 0.001; and **** P < 0.0001; n.s.= not significant.

Article Snippet: OVA peptide (OVA 323-339, InvivoGen) was introduced to stimulate the cocultures.

Techniques: Expressing, Fluorescence, Negative Control