ovalbumin  (InvivoGen)

Journal: bioRxiv

Article Title: Engulfment by brain macrophages in a short-lived vertebrate

doi: 10.64898/2026.04.28.721434

Figure Lengend Snippet: a, (Left) Representative flow cytometry histograms from young adult (41 days, above) and old (160 days, below) male heterozygous SP-oScarlet killifish brains, highlighting mean oScarlet fluorescence intensity in oScarlet HIGH cells. (Right) Quantification of mean oScarlet fluorescence intensity among young adult and old oScarlet HIGH cells (n = 6 fish per age per sex, over two experiments per sex; young adult males: 41-63 days; old males: 148-160 days; young adult females: 57-63 days; old females: 161-167 days). Each square (male) or triangle (female) represents one fish. Mean +/- standard error of mean; p-values, unpaired Wilcoxon rank-sum test at per-animal level. b, Schematic for ex vivo engulfment assays performed on young adult and old heterozygous SP-oScarlet killifish brains (for ovalbumin-Alexa Fluor ® 647: n = 6 fish per age and sex, over two experiments per sex; young adult males: 41-63 days; old males: 148-160 days; young adult females: 57-63 days; old females: 161-167 days; for dextran-Oregon Green 488 (70 kDa): n = 4 male fish per age in one experiment; young adult: 56 days; old: 156 days). c, (Left) Representative flow cytometry histograms from young adult (41 days) male heterozygous SP-oScarlet killifish brains, highlighting mean ovalbumin fluorescence intensity in oScarlet LOW and oScarlet HIGH cells. (Right) Quantification of mean ovalbumin fluorescence intensity among young adult oScarlet LOW and oScarlet HIGH cells (same experiments as in a ). Each square (male) or triangle (female) represents one fish. Mean +/- standard error of mean; p-values, paired Wilcoxon rank-sum test at per-animal level. d, (Left) Representative flow cytometry histograms from young adult (41 days) (above) and old (160 days) (below) male heterozygous SP-oScarlet killifish brains, highlighting mean ovalbumin fluorescence intensity in oScarlet HIGH cells. (Right) Quantification of mean ovalbumin fluorescence intensity among young adult and old oScarlet HIGH cells (same experiments as in a ). Each square (male) or triangle (female) represents one fish. Mean +/- standard error of mean; p-values, unpaired Wilcoxon rank-sum test at per-animal level. e, (Left) Representative flow cytometry histograms from young adult (56 days) male heterozygous SP-oScarlet killifish brains, highlighting mean dextran fluorescence intensity in oScarlet LOW and oScarlet HIGH cells. (Right) Quantification of mean dextran fluorescence intensity among young adult oScarlet LOW and oScarlet HIGH cells. Each square represents one fish. Mean +/- standard error of mean; p-values, paired Wilcoxon rank-sum test at per-animal level. f, (Left) Representative flow cytometry histograms from young adult (56 days) (above) and old (156 days) (below) male heterozygous SP-oScarlet killifish brains, highlighting mean dextran fluorescence intensity in oScarlet HIGH cells. Each dot represents one cell. (Right) Quantification of mean dextran fluorescence intensity among young adult and old oScarlet HIGH cells (same experiment as in e ). Each square represents one fish. Mean +/-standard error of mean; p-values, unpaired Wilcoxon rank-sum test at per-animal level.

Article Snippet: To assess acute endocytic capacity of killifish brain macrophages, we performed ex vivo engulfment of ovalbumin-Alexa Fluor ® 647 (a 45 kDa protein) (Fisher Scientific, O34784) as well as dextran-Oregon Green 488 (a 70 kDa polysaccharide) (Thermo Fisher Scientific, D7173).

Techniques: Flow Cytometry, Fluorescence, Ex Vivo