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proteinase k  (MedChemExpress)


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    Structured Review

    MedChemExpress proteinase k
    ASCs-ApoVs exhibited cross-species conservation and robust environmental resilience. ( A ) Western blot analysis of myofibroblast-related markers in HS-fibroblasts with freezing-thawing (for 3 times) ASCs-ApoVs, freezing-thawing ASCs-ApoVs with additional DNase I treatment, freezing-thawing ASCs-ApoVs with additional RNase A treatment. ( B ) Quantification of ( a ) COL1A1, ( b ) COL3A1, ( c ) COL3A1/COL1A1, ( d ) α-SMA, ( e ) CDC20, ( f ) Vimentin, and ( g ) TGF-β1 expression from ( A ) section by relative gray vale to GAPDH and the control group. (Mean ± SD, n = 3 independent experiments, data analyzed by one-way ANOVA with Tukey’s HSD.) ( C ) RT-qPCR results of ( a ) COL1A1 , ( b ) COL3A1 , ( c ) COL3A1/COL1A1 , ( d ) ACTA2 , ( e ) CDC20 , ( f ) VIM , and ( g ) TGFB1 in HS-fibroblasts with processed ASCs-ApoVs treatment (ASCs-ApoVs freezing-thawing for 3 times, freezing-thawing ASCs-ApoVs with additional DNase I treatment, freezing-thawing ASCs-ApoVs with additional RNase A treatment, freezing-thawing ASCs-ApoVs with additional <t>Proteinase</t> <t>K</t> treatment), normalized to GAPDH and the control group. (Mean ± SD, n = 3 independent experiments, data analyzed by one-way ANOVA with Tukey’s HSD.) ( D ) Western blot analysis of CDC20 expression in normal human fibroblasts (BJ-fibroblasts), human keratinocytes (HaCaT cells), and mouse oral mucosa epithelial cells. ( E ) Quantification of CDC20 expression from ( D ) section by relative gray vale to GAPDH/β-actin. (Mean ± SD, n = 3 independent experiments, data analyzed by Independent-samples t -test.) Statistical significance: ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
    Proteinase K, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/otenabant/Otenabant/pmc13204812-143-16-18
    Average 94 stars, based on 4 article reviews
    proteinase k - by Bioz Stars, 2026-09
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    Images

    1) Product Images from "Adipose Stem Cell-Derived Apoptotic Vesicles Attenuate Hypertrophic Scarring by Targeting the CDC20/WNT Signaling Pathway"

    Article Title: Adipose Stem Cell-Derived Apoptotic Vesicles Attenuate Hypertrophic Scarring by Targeting the CDC20/WNT Signaling Pathway

    Journal: Biomedicines

    doi: 10.3390/biomedicines14051083

    ASCs-ApoVs exhibited cross-species conservation and robust environmental resilience. ( A ) Western blot analysis of myofibroblast-related markers in HS-fibroblasts with freezing-thawing (for 3 times) ASCs-ApoVs, freezing-thawing ASCs-ApoVs with additional DNase I treatment, freezing-thawing ASCs-ApoVs with additional RNase A treatment. ( B ) Quantification of ( a ) COL1A1, ( b ) COL3A1, ( c ) COL3A1/COL1A1, ( d ) α-SMA, ( e ) CDC20, ( f ) Vimentin, and ( g ) TGF-β1 expression from ( A ) section by relative gray vale to GAPDH and the control group. (Mean ± SD, n = 3 independent experiments, data analyzed by one-way ANOVA with Tukey’s HSD.) ( C ) RT-qPCR results of ( a ) COL1A1 , ( b ) COL3A1 , ( c ) COL3A1/COL1A1 , ( d ) ACTA2 , ( e ) CDC20 , ( f ) VIM , and ( g ) TGFB1 in HS-fibroblasts with processed ASCs-ApoVs treatment (ASCs-ApoVs freezing-thawing for 3 times, freezing-thawing ASCs-ApoVs with additional DNase I treatment, freezing-thawing ASCs-ApoVs with additional RNase A treatment, freezing-thawing ASCs-ApoVs with additional Proteinase K treatment), normalized to GAPDH and the control group. (Mean ± SD, n = 3 independent experiments, data analyzed by one-way ANOVA with Tukey’s HSD.) ( D ) Western blot analysis of CDC20 expression in normal human fibroblasts (BJ-fibroblasts), human keratinocytes (HaCaT cells), and mouse oral mucosa epithelial cells. ( E ) Quantification of CDC20 expression from ( D ) section by relative gray vale to GAPDH/β-actin. (Mean ± SD, n = 3 independent experiments, data analyzed by Independent-samples t -test.) Statistical significance: ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
    Figure Legend Snippet: ASCs-ApoVs exhibited cross-species conservation and robust environmental resilience. ( A ) Western blot analysis of myofibroblast-related markers in HS-fibroblasts with freezing-thawing (for 3 times) ASCs-ApoVs, freezing-thawing ASCs-ApoVs with additional DNase I treatment, freezing-thawing ASCs-ApoVs with additional RNase A treatment. ( B ) Quantification of ( a ) COL1A1, ( b ) COL3A1, ( c ) COL3A1/COL1A1, ( d ) α-SMA, ( e ) CDC20, ( f ) Vimentin, and ( g ) TGF-β1 expression from ( A ) section by relative gray vale to GAPDH and the control group. (Mean ± SD, n = 3 independent experiments, data analyzed by one-way ANOVA with Tukey’s HSD.) ( C ) RT-qPCR results of ( a ) COL1A1 , ( b ) COL3A1 , ( c ) COL3A1/COL1A1 , ( d ) ACTA2 , ( e ) CDC20 , ( f ) VIM , and ( g ) TGFB1 in HS-fibroblasts with processed ASCs-ApoVs treatment (ASCs-ApoVs freezing-thawing for 3 times, freezing-thawing ASCs-ApoVs with additional DNase I treatment, freezing-thawing ASCs-ApoVs with additional RNase A treatment, freezing-thawing ASCs-ApoVs with additional Proteinase K treatment), normalized to GAPDH and the control group. (Mean ± SD, n = 3 independent experiments, data analyzed by one-way ANOVA with Tukey’s HSD.) ( D ) Western blot analysis of CDC20 expression in normal human fibroblasts (BJ-fibroblasts), human keratinocytes (HaCaT cells), and mouse oral mucosa epithelial cells. ( E ) Quantification of CDC20 expression from ( D ) section by relative gray vale to GAPDH/β-actin. (Mean ± SD, n = 3 independent experiments, data analyzed by Independent-samples t -test.) Statistical significance: ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Techniques Used: Western Blot, Expressing, Control, Quantitative RT-PCR

    Related Articles

    Fluorescence:

    Article Title: Pharmacological Evaluation of Cannabinoid Receptor Modulators Using GRAB eCB2.0 Sensor
    Article Snippet: activity of the receptors in such modulators. Chemicals and Reagents: 2-AG (Cayman, Ann Arbor, MI, USA #62160), CP55940 (Cayman #13608), ACEA (Biotechne, Minneapolis, MN, USA: Tocris #1319), SR141716 (MedChem Express, New York, NY, USA: #HY14136), Taranabant (MedChem Express #HY10013), Otenabant (MedChem Express #HY-10871), Ibipinabant (MedChem Express HY-14791), and GAT228 and GAT229 (MilliporeSig

    Article Title: Pharmacological Evaluation of Cannabinoid Receptor Modulators Using GRAB eCB2.0 Sensor
    Article Snippet: Chemicals and Reagents: 2-AG (Cayman, Ann Arbor, MI, USA #62160), CP55940 (Cayman #13608), ACEA (Biotechne, Minneapolis, MN, USA: Tocris #1319), SR141716 (MedChem Express, New York, NY, USA: #HY14136), Taranabant (MedChem Express #HY10013), Otenabant (MedChem Express #HY-10871), Ibipinabant (MedChem Express HY-14791), and GAT228 and GAT229 (MilliporeSigma: Burlington, MA, USA #SML1952 and #SML1951).. ORG27569 (Tocris #2957), PSNCBAM-1 (Cayman #25855), Pregnenolone and Pregnenolone sulfate (Thermo Fisher, Waltham, MA, USA: #AC164420050 and # 537650), JD5037, MRI-1867, MRI-1891, MRI-1776, MRI-1887, MRI-2213, MRI-2594, MRI-2687, and MRI-2006 (structures of compounds in ) were synthesized in-house according to previously published procedures [ , , , , , , ].ORG27569 (Tocris #2957), PSNCBAM-1 (Cayman #25855), Pregnenolone and Pregnenolone sulfate (Thermo Fisher, Waltham, MA, USA: #AC164420050 and # 537650), JD5037, MRI-1867, MRI-1891, MRI-1776, MRI-1887, MRI-2213, MRI-2594, MRI-2687, and MRI-2006 (structures of compounds in ) were synthesized in-house according..



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    Image Search Results


    ASCs-ApoVs exhibited cross-species conservation and robust environmental resilience. ( A ) Western blot analysis of myofibroblast-related markers in HS-fibroblasts with freezing-thawing (for 3 times) ASCs-ApoVs, freezing-thawing ASCs-ApoVs with additional DNase I treatment, freezing-thawing ASCs-ApoVs with additional RNase A treatment. ( B ) Quantification of ( a ) COL1A1, ( b ) COL3A1, ( c ) COL3A1/COL1A1, ( d ) α-SMA, ( e ) CDC20, ( f ) Vimentin, and ( g ) TGF-β1 expression from ( A ) section by relative gray vale to GAPDH and the control group. (Mean ± SD, n = 3 independent experiments, data analyzed by one-way ANOVA with Tukey’s HSD.) ( C ) RT-qPCR results of ( a ) COL1A1 , ( b ) COL3A1 , ( c ) COL3A1/COL1A1 , ( d ) ACTA2 , ( e ) CDC20 , ( f ) VIM , and ( g ) TGFB1 in HS-fibroblasts with processed ASCs-ApoVs treatment (ASCs-ApoVs freezing-thawing for 3 times, freezing-thawing ASCs-ApoVs with additional DNase I treatment, freezing-thawing ASCs-ApoVs with additional RNase A treatment, freezing-thawing ASCs-ApoVs with additional Proteinase K treatment), normalized to GAPDH and the control group. (Mean ± SD, n = 3 independent experiments, data analyzed by one-way ANOVA with Tukey’s HSD.) ( D ) Western blot analysis of CDC20 expression in normal human fibroblasts (BJ-fibroblasts), human keratinocytes (HaCaT cells), and mouse oral mucosa epithelial cells. ( E ) Quantification of CDC20 expression from ( D ) section by relative gray vale to GAPDH/β-actin. (Mean ± SD, n = 3 independent experiments, data analyzed by Independent-samples t -test.) Statistical significance: ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: Biomedicines

    Article Title: Adipose Stem Cell-Derived Apoptotic Vesicles Attenuate Hypertrophic Scarring by Targeting the CDC20/WNT Signaling Pathway

    doi: 10.3390/biomedicines14051083

    Figure Lengend Snippet: ASCs-ApoVs exhibited cross-species conservation and robust environmental resilience. ( A ) Western blot analysis of myofibroblast-related markers in HS-fibroblasts with freezing-thawing (for 3 times) ASCs-ApoVs, freezing-thawing ASCs-ApoVs with additional DNase I treatment, freezing-thawing ASCs-ApoVs with additional RNase A treatment. ( B ) Quantification of ( a ) COL1A1, ( b ) COL3A1, ( c ) COL3A1/COL1A1, ( d ) α-SMA, ( e ) CDC20, ( f ) Vimentin, and ( g ) TGF-β1 expression from ( A ) section by relative gray vale to GAPDH and the control group. (Mean ± SD, n = 3 independent experiments, data analyzed by one-way ANOVA with Tukey’s HSD.) ( C ) RT-qPCR results of ( a ) COL1A1 , ( b ) COL3A1 , ( c ) COL3A1/COL1A1 , ( d ) ACTA2 , ( e ) CDC20 , ( f ) VIM , and ( g ) TGFB1 in HS-fibroblasts with processed ASCs-ApoVs treatment (ASCs-ApoVs freezing-thawing for 3 times, freezing-thawing ASCs-ApoVs with additional DNase I treatment, freezing-thawing ASCs-ApoVs with additional RNase A treatment, freezing-thawing ASCs-ApoVs with additional Proteinase K treatment), normalized to GAPDH and the control group. (Mean ± SD, n = 3 independent experiments, data analyzed by one-way ANOVA with Tukey’s HSD.) ( D ) Western blot analysis of CDC20 expression in normal human fibroblasts (BJ-fibroblasts), human keratinocytes (HaCaT cells), and mouse oral mucosa epithelial cells. ( E ) Quantification of CDC20 expression from ( D ) section by relative gray vale to GAPDH/β-actin. (Mean ± SD, n = 3 independent experiments, data analyzed by Independent-samples t -test.) Statistical significance: ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: The temperature-cycled ASCs-ApoVs were then aliquoted into four treatment groups: 1 PBS control, 2 50 μg/mL proteinase K (MCE, HY-10871), 3 50 μg/mL RNase A (MCE, HY-129046A), and 4 8 μg/mL DNase I (Beyotime, D7073; 37 °C, 60 min incubation).

    Techniques: Western Blot, Expressing, Control, Quantitative RT-PCR

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    Journal: International Journal of Molecular Sciences

    Article Title: Pharmacological Evaluation of Cannabinoid Receptor Modulators Using GRAB eCB2.0 Sensor

    doi: 10.3390/ijms25095012

    Figure Lengend Snippet: Effect of 3-arm antagonists in modulating GRAB eCB2.0 fluorescence in presence of 300 nM CP55940.

    Article Snippet: Chemicals and Reagents: 2-AG (Cayman, Ann Arbor, MI, USA #62160), CP55940 (Cayman #13608), ACEA (Biotechne, Minneapolis, MN, USA: Tocris #1319), SR141716 (MedChem Express, New York, NY, USA: #HY14136), Taranabant (MedChem Express #HY10013), Otenabant (MedChem Express #HY-10871), Ibipinabant (MedChem Express HY-14791), and GAT228 and GAT229 (MilliporeSigma: Burlington, MA, USA #SML1952 and #SML1951).

    Techniques: Fluorescence

    Update of major metabolic targets and potential monotherapy drugs under development for NAFLD/NASH

    Journal: Hepatology Communications

    Article Title: Overview and prospect of NAFLD: Significant roles of nutrients and dietary patterns in its progression or prevention

    doi: 10.1097/HC9.0000000000000234

    Figure Lengend Snippet: Update of major metabolic targets and potential monotherapy drugs under development for NAFLD/NASH

    Article Snippet: , Otenabant (CP-945598) , Phase 1 , NASH , √ , NA , NA , NA , Pfizer , Aronne et al .

    Techniques: