ocn  (Proteintech)

Journal: Bioactive Materials

Article Title: Bacteria-responsive DNAgel system for targeted delivery of photothermally enhanced MXene/MoS 2 in the treatment of pyogenic osteomyelitis

doi: 10.1016/j.bioactmat.2025.10.023

Figure Lengend Snippet: MXMoS 2 DNAgel effectively accelerates bone regeneration and suppresses infection in pyogenic osteomyelitis. (A) Schematic diagram of the treatment procedure of suppurative osteomyelitis mice. (B) Bacterial colonies in mice wounds before and after NIR treatment. (C) Quantification of bacterial colony counts. (D) 3D micro-CT reconstruction of maxilla at 4 weeks after different treatment. Representative images of H&E and Masson's trichrome staining of the maxilla at 4 weeks treatment. Immunofluorescence staining for IL-6 (red), TRAP (red) and OCN (red) in maxilla. Nuclei were counterstained with DAPI (blue). (E) BMD and (F) BV/TV analyses of newly formed bone calculated based on micro-CT. (G – I) Quantitative analysis of the ROI. Data are presented as mean ± SD (n = 5 mice per group). ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001.

Article Snippet: Primary antibodies for immunocytochemistry and immunofluorescence staining—including ARG1 and iNOS, TRAP, β-Catenin, OCN and WNT4—were obtained from Proteintech (China).

Techniques: Infection, Micro-CT, Staining, Immunofluorescence

Journal: Bioactive Materials

Article Title: Biomineralization-inspired scaffolds using citrate-based polymers to stabilize amorphous calcium phosphate promote osteogenesis and angiogenesis for bone defect repair

doi: 10.1016/j.bioactmat.2025.10.016

Figure Lengend Snippet: Biocompatibility and osteogenic activity of POC-ACP scaffolds under different polymerization conditions in vitro . A) Schematic diagram of rBMSC cultured with different scaffolds conditioned medium for the evaluation of osteogenic activity. B) Live/Dead staining of rBMSCs cultured with different POC-ACP scaffolds conditioned medium (Scale bar = 200 μm). C) ALP staining of rBMSCs cultured with different POC-ACP scaffolds conditioned medium at day 7 (Scale bar = 5 mm and 200 μm, respectively). D) ARS staining of rBMSCs cultured with different POC-ACP scaffolds conditioned medium at day 14 (Scale bar = 5 mm and 200 μm, respectively). E) The relative mRNA expression of osteogenic-related genes COL1, RUNX2 and OCN in rBMSCs cultured in different POC-ACP scaffolds-conditioned medium (n = 3). F) The osteogenic-related proteins expression COL1, RUNX2 and OCN in rBMSCs cultured in different POC-ACP scaffolds-conditioned medium. G) Quantitative analysis of RUNX2 protein expression levels in Western blot (n = 3). H) Schematic diagram of rBMSC cultured on different scaffolds for the evaluation of osteogenic activity. I) SEM images of rBMSCs cultured on different POC-ACP scaffolds (Scale bar = 20 μm). J) ALP staining of rBMSCs cultured on different POC-ACP scaffolds at day 7 (Scale bar = 1 cm). K) ARS staining of rBMSCs cultured on different POC-ACP scaffolds at day 14 (Scale bar = 1 cm). L) The relative mRNA expression of osteogenic-related genes COL1, RUNX2 and OCN in rBMSCs cultured on different POC-ACP scaffolds (n = 3). M) The osteogenic-related proteins expression COL1, RUNX2 and OCN in rBMSCs cultured on different POC-ACP scaffolds. N) Quantitative analysis of RUNX2 protein expression levels in Western blot (n = 3). Error bars, mean ± standard deviation, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: IHC staining was performed on femur sections μm thick with primary antibodies against OCN (1:200, Proteintech, China) and CD31 (1:200, Proteintech, China), according to the described protocol [ ].

Techniques: Activity Assay, In Vitro, Cell Culture, Staining, Expressing, Western Blot, Standard Deviation

Journal: Bioactive Materials

Article Title: Biomineralization-inspired scaffolds using citrate-based polymers to stabilize amorphous calcium phosphate promote osteogenesis and angiogenesis for bone defect repair

doi: 10.1016/j.bioactmat.2025.10.016

Figure Lengend Snippet: POC-ACP promoted osteogenic differentiation compared to POC-HA scaffolds in vitro . A) Schematic diagram of rBMSC cultured with different scaffolds conditioned medium for the evaluation of osteogenic activity. B) ALP staining of rBMSCs cultured with different conditioned medium at day 7 (Scale bar = 5 mm and 200 μm, respectively). C) ARS staining of rBMSCs cultured with different conditioned medium at day 14 (Scale bar = 5 mm and 200 μm, respectively). D) The relative mRNA expression of osteogenic-related genes COL1, RUNX2 and OCN in rBMSCs cultured in different conditioned medium (n = 3). E) The osteogenic-related proteins expression COL1, RUNX2 and OCN in rBMSCs cultured in different conditioned medium. F) Quantitative analysis of RUNX2 protein expression levels in Western blot (n = 3). G) Schematic diagram of rBMSC cultured on different scaffolds for the evaluation of osteogenic activity. H) ALP staining of rBMSCs cultured on different scaffolds at day 7 (Scale bar = 1 cm). I) ARS staining of rBMSCs cultured on different scaffolds at day 14 (Scale bar = 1 cm). J) The relative mRNA expression of osteogenic-related genes COL1, RUNX2 and OCN in rBMSCs cultured on different scaffolds (n = 3). K) The osteogenic-related proteins expression COL1, RUNX2 and OCN in rBMSCs cultured on different scaffolds. L) Quantitative analysis of RUNX2 protein expression levels in Western blot (n = 3). The POC-ACP group indicates the POC-ACP3 (80 °C, 3 d) group. Error bars, mean ± standard deviation, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: IHC staining was performed on femur sections μm thick with primary antibodies against OCN (1:200, Proteintech, China) and CD31 (1:200, Proteintech, China), according to the described protocol [ ].

Techniques: In Vitro, Cell Culture, Activity Assay, Staining, Expressing, Western Blot, Standard Deviation

Journal: Bioactive Materials

Article Title: Biomineralization-inspired scaffolds using citrate-based polymers to stabilize amorphous calcium phosphate promote osteogenesis and angiogenesis for bone defect repair

doi: 10.1016/j.bioactmat.2025.10.016

Figure Lengend Snippet: Histological staining analysis in vivo . A) Histological staining of new bone tissue by HE and Masson staining (Scale bar = 100 μm and 200 μm, respectively). B) HE staining of heart, liver, spleen, lung and kidney tissues (Scale bar = 50 μm). C Immunohistochemical staining of osteogenic (OCN) and angiogenic (CD31) in new bone tissue (Scale bar = 100 μm and 200 μm, respectively). D) Quantitative analysis of the number of OCN-positive cells (n = 3). E) Quantitative analysis of the number of CD31-positive cells (n = 3). The POC-ACP group indicates the POC-ACP3 (80 °C, 3 d) group. Error bars, mean ± standard deviation, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: IHC staining was performed on femur sections μm thick with primary antibodies against OCN (1:200, Proteintech, China) and CD31 (1:200, Proteintech, China), according to the described protocol [ ].

Techniques: Staining, In Vivo, Immunohistochemical staining, Standard Deviation

Journal: iScience

Article Title: Calcium carbonate supplementation exacerbated gut microenvironment disruption in senile osteoporosis mice

doi: 10.1016/j.isci.2025.114530

Figure Lengend Snippet: In senile osteoporotic mice, calcium carbonate supplementation did not significantly improve the osteogenic differentiation of BMMSCs (A) Flow chart of the extraction of BMMSCs from the bone marrow cavity and osteoblast differentiation-related tests. (B and C) Representative images and quantitative results of ALP and ARS staining during osteogenic differentiation in different groups. (D) qRT-PCR was performed to analyze the mRNA levels of osteogenic-related genes ( Runx2 and Ocn ) in BMMSCs during the osteoblast differentiation. (E) Western blotting was performed to analyze the protein levels of RUNX2 and OCN. n = 3/all group, n represents the number of independent BMMSC samples (each sample isolated from an individual mouse). The values are mean ± SD of at least 3 independent experiments, n.s. p > 0.05, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Equal amounts of protein samples were separated on polyacrylamide gels, transferred to nitrocellulose membranes, and blocked with 5% skim milk powder for 1 h. The membranes obtained were incubated with anti-RUNX2 antibody (#20700-1-AP, Proteintech, China), and anti-OCN antibody (#23418-1-AP, Proteintech, China) was incubated at 4°C overnight.

Techniques: Calcium Carbonate, Extraction, Staining, Quantitative RT-PCR, Western Blot, Isolation

Journal: iScience

Article Title: Calcium carbonate supplementation exacerbated gut microenvironment disruption in senile osteoporosis mice

doi: 10.1016/j.isci.2025.114530

Figure Lengend Snippet: Transplantation of gut microbiota from senile mice supplemented with calcium carbonate did not significantly enhance the osteogenic differentiation capacity of BMMSCs (A) Flow chart of the extraction of BMMSCs from the bone marrow cavity and osteoblast differentiation-related tests. (B and C) Representative images and quantitative results of ALP and ARS staining during osteogenic differentiation in different groups. (D) qRT-PCR was performed to analyze the mRNA levels of osteogenic-related genes ( Runx2 and Ocn ) in BMMSCs during the osteoblast differentiation. (E) Western blotting was performed to analyze the protein levels of RUNX2 and OCN. n = 3/all group, n represents the number of independent BMMSC samples (each sample isolated from an individual mouse). The values are mean ± SD of at least 3 independent experiments, n.s. p > 0.05, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Equal amounts of protein samples were separated on polyacrylamide gels, transferred to nitrocellulose membranes, and blocked with 5% skim milk powder for 1 h. The membranes obtained were incubated with anti-RUNX2 antibody (#20700-1-AP, Proteintech, China), and anti-OCN antibody (#23418-1-AP, Proteintech, China) was incubated at 4°C overnight.

Techniques: Transplantation Assay, Calcium Carbonate, Extraction, Staining, Quantitative RT-PCR, Western Blot, Isolation

Journal: iScience

Article Title: Calcium carbonate supplementation exacerbated gut microenvironment disruption in senile osteoporosis mice

doi: 10.1016/j.isci.2025.114530

Figure Lengend Snippet: In senile osteoporotic mice, calcium carbonate supplementation did not significantly improve the osteogenic differentiation of BMMSCs (A) Flow chart of the extraction of BMMSCs from the bone marrow cavity and osteoblast differentiation-related tests. (B and C) Representative images and quantitative results of ALP and ARS staining during osteogenic differentiation in different groups. (D) qRT-PCR was performed to analyze the mRNA levels of osteogenic-related genes ( Runx2 and Ocn ) in BMMSCs during the osteoblast differentiation. (E) Western blotting was performed to analyze the protein levels of RUNX2 and OCN. n = 3/all group, n represents the number of independent BMMSC samples (each sample isolated from an individual mouse). The values are mean ± SD of at least 3 independent experiments, n.s. p > 0.05, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: OCN Antibody , Proteintech , Cat# 23418-1-AP; RRID: AB_2879275.

Techniques: Calcium Carbonate, Extraction, Staining, Quantitative RT-PCR, Western Blot, Isolation

Journal: iScience

Article Title: Calcium carbonate supplementation exacerbated gut microenvironment disruption in senile osteoporosis mice

doi: 10.1016/j.isci.2025.114530

Figure Lengend Snippet: Transplantation of gut microbiota from senile mice supplemented with calcium carbonate did not significantly enhance the osteogenic differentiation capacity of BMMSCs (A) Flow chart of the extraction of BMMSCs from the bone marrow cavity and osteoblast differentiation-related tests. (B and C) Representative images and quantitative results of ALP and ARS staining during osteogenic differentiation in different groups. (D) qRT-PCR was performed to analyze the mRNA levels of osteogenic-related genes ( Runx2 and Ocn ) in BMMSCs during the osteoblast differentiation. (E) Western blotting was performed to analyze the protein levels of RUNX2 and OCN. n = 3/all group, n represents the number of independent BMMSC samples (each sample isolated from an individual mouse). The values are mean ± SD of at least 3 independent experiments, n.s. p > 0.05, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: OCN Antibody , Proteintech , Cat# 23418-1-AP; RRID: AB_2879275.

Techniques: Transplantation Assay, Calcium Carbonate, Extraction, Staining, Quantitative RT-PCR, Western Blot, Isolation