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ATCC hcov oc43

Cytotoxicity induced <t>by</t> <t>HCoV-OC43</t> infection in human colon and lung cell lines. Viral concentrations correspond to a 1:2 serial dilution, where 1 represents the undiluted viral inoculum. For all data, n ≥ 3 (biological replicates). + Significantly different from uninfected cells ( p < 0.05). * Significantly different from MRC-5 cells ( p < 0.05). Data are representative of three separate experimental runs.

Hcov Oc43, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 438 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 98 stars, based on 438 article reviews

hcov oc43 - by Bioz Stars, 2026-06

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1) Product Images from "Antiviral Activity of Essential Oil from Populus balsamifera L. Buds and Its Major Compounds Against Betacoronavirus HCoV-OC43 Using a Sensitive Cytoprotection Assay"

Article Title: Antiviral Activity of Essential Oil from Populus balsamifera L. Buds and Its Major Compounds Against Betacoronavirus HCoV-OC43 Using a Sensitive Cytoprotection Assay

Journal: Molecules

doi: 10.3390/molecules31091496

Cytotoxicity induced by HCoV-OC43 infection in human colon and lung cell lines. Viral concentrations correspond to a 1:2 serial dilution, where 1 represents the undiluted viral inoculum. For all data, n ≥ 3 (biological replicates). + Significantly different from uninfected cells ( p < 0.05). * Significantly different from MRC-5 cells ( p < 0.05). Data are representative of three separate experimental runs.

Figure Legend Snippet: Cytotoxicity induced by HCoV-OC43 infection in human colon and lung cell lines. Viral concentrations correspond to a 1:2 serial dilution, where 1 represents the undiluted viral inoculum. For all data, n ≥ 3 (biological replicates). + Significantly different from uninfected cells ( p < 0.05). * Significantly different from MRC-5 cells ( p < 0.05). Data are representative of three separate experimental runs.

Techniques Used: Infection, Serial Dilution

Antiviral activity of positive controls against MRC-5 infected with increasing concentrations of HCoV-OC43. The viral curve corresponds to MRC-5 cells infected with increasing concentrations of HCoV-OC43 without treatment with positive controls. Chloroquine, hydroxychloroquine and molnupiravir provided strong cytoprotection against viral infection, whereas fluvoxamine lost efficacy at higher viral concentrations. For all data, n ≥ 3 (biological replicates). * Significantly different from the viral curve ( p < 0.05). Data are representative of three separate experimental runs.

Figure Legend Snippet: Antiviral activity of positive controls against MRC-5 infected with increasing concentrations of HCoV-OC43. The viral curve corresponds to MRC-5 cells infected with increasing concentrations of HCoV-OC43 without treatment with positive controls. Chloroquine, hydroxychloroquine and molnupiravir provided strong cytoprotection against viral infection, whereas fluvoxamine lost efficacy at higher viral concentrations. For all data, n ≥ 3 (biological replicates). * Significantly different from the viral curve ( p < 0.05). Data are representative of three separate experimental runs.

Techniques Used: Activity Assay, Infection

Antiviral activity of chloroquine against MRC-5 cells infected with HCoV-OC43. MRC-5 cells were treated with increasing concentrations of chloroquine (0.04–1.25 µg/mL). The viral curve (VC) corresponds to MRC-5 cells infected with HCoV-OC43 without chloroquine. For all data, n ≥ 3 (biological replicates). Data are representative of three separate experimental runs.

Figure Legend Snippet: Antiviral activity of chloroquine against MRC-5 cells infected with HCoV-OC43. MRC-5 cells were treated with increasing concentrations of chloroquine (0.04–1.25 µg/mL). The viral curve (VC) corresponds to MRC-5 cells infected with HCoV-OC43 without chloroquine. For all data, n ≥ 3 (biological replicates). Data are representative of three separate experimental runs.

Techniques Used: Activity Assay, Infection

Determination of half maximum effective concentration (EC 50 ) of chloroquine against MRC-5 infection with HCoV-OC43. The non-linear sigmoidal regression was obtained using AUC c calculated from chloroquine. Growing concentrations ranged from 0.04 to 1.5 µg/mL. The EC 50 values (cytoprotection against virus-induced cell death) were derived using a non-linear sigmoidal regression equation. For all data, n ≥ 3 (biological replicates). Data are representative of three separate experimental runs.

Figure Legend Snippet: Determination of half maximum effective concentration (EC 50 ) of chloroquine against MRC-5 infection with HCoV-OC43. The non-linear sigmoidal regression was obtained using AUC c calculated from chloroquine. Growing concentrations ranged from 0.04 to 1.5 µg/mL. The EC 50 values (cytoprotection against virus-induced cell death) were derived using a non-linear sigmoidal regression equation. For all data, n ≥ 3 (biological replicates). Data are representative of three separate experimental runs.

Techniques Used: Concentration Assay, Infection, Virus, Derivative Assay

Antiviral activity of P. balsamifera essential oil. MRC-5 cells were infected with growing concentrations of HCoV-OC43 and treated at concentrations ranged from between 0.2 and 14.4 µg/mL of P. balsamifera essential oil. The EC 50 value (cytoprotection against virus-induced cell death) was then calculated from the generated dose–response curves. The control is represented by the viral curve (VC). For all data, n ≥ 3 (biological replicates). Data are representative of three separate experimental runs.

Figure Legend Snippet: Antiviral activity of P. balsamifera essential oil. MRC-5 cells were infected with growing concentrations of HCoV-OC43 and treated at concentrations ranged from between 0.2 and 14.4 µg/mL of P. balsamifera essential oil. The EC 50 value (cytoprotection against virus-induced cell death) was then calculated from the generated dose–response curves. The control is represented by the viral curve (VC). For all data, n ≥ 3 (biological replicates). Data are representative of three separate experimental runs.

Techniques Used: Activity Assay, Infection, Virus, Generated, Control

Antiviral activity of α-bisabolol ( A ) and nerolidol ( B ). MRC-5 cells were infected with growing concentrations of HCoV-OC43 and treated at different concentrations of α-bisabolol or nerolidol. Concentrations ranged between 0.2 and 14.4 µg/mL for both molecules. The EC 50 value (cytoprotection against virus-induced cell death) was then calculated from the generated dose–response curves. The control is represented by the viral curve (VC). For all data, n ≥ 3 (biological replicates). Data are representative of three separate experimental runs.

Figure Legend Snippet: Antiviral activity of α-bisabolol ( A ) and nerolidol ( B ). MRC-5 cells were infected with growing concentrations of HCoV-OC43 and treated at different concentrations of α-bisabolol or nerolidol. Concentrations ranged between 0.2 and 14.4 µg/mL for both molecules. The EC 50 value (cytoprotection against virus-induced cell death) was then calculated from the generated dose–response curves. The control is represented by the viral curve (VC). For all data, n ≥ 3 (biological replicates). Data are representative of three separate experimental runs.

Techniques Used: Activity Assay, Infection, Virus, Generated, Control

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Journal: Molecules

Article Title: Antiviral Activity of Essential Oil from Populus balsamifera L. Buds and Its Major Compounds Against Betacoronavirus HCoV-OC43 Using a Sensitive Cytoprotection Assay

doi: 10.3390/molecules31091496

Figure Lengend Snippet: Cytotoxicity induced by HCoV-OC43 infection in human colon and lung cell lines. Viral concentrations correspond to a 1:2 serial dilution, where 1 represents the undiluted viral inoculum. For all data, n ≥ 3 (biological replicates). + Significantly different from uninfected cells ( p < 0.05). * Significantly different from MRC-5 cells ( p < 0.05). Data are representative of three separate experimental runs.

Article Snippet: HCT-8 cells were used for viral replication of HCoV-OC43 (VR-1558), as recommended by the ATCC.

Techniques: Infection, Serial Dilution

Journal: Molecules

Article Title: Antiviral Activity of Essential Oil from Populus balsamifera L. Buds and Its Major Compounds Against Betacoronavirus HCoV-OC43 Using a Sensitive Cytoprotection Assay

doi: 10.3390/molecules31091496

Figure Lengend Snippet: Antiviral activity of positive controls against MRC-5 infected with increasing concentrations of HCoV-OC43. The viral curve corresponds to MRC-5 cells infected with increasing concentrations of HCoV-OC43 without treatment with positive controls. Chloroquine, hydroxychloroquine and molnupiravir provided strong cytoprotection against viral infection, whereas fluvoxamine lost efficacy at higher viral concentrations. For all data, n ≥ 3 (biological replicates). * Significantly different from the viral curve ( p < 0.05). Data are representative of three separate experimental runs.

Article Snippet: HCT-8 cells were used for viral replication of HCoV-OC43 (VR-1558), as recommended by the ATCC.

Techniques: Activity Assay, Infection

Journal: Molecules

Article Title: Antiviral Activity of Essential Oil from Populus balsamifera L. Buds and Its Major Compounds Against Betacoronavirus HCoV-OC43 Using a Sensitive Cytoprotection Assay

doi: 10.3390/molecules31091496

Figure Lengend Snippet: Antiviral activity of chloroquine against MRC-5 cells infected with HCoV-OC43. MRC-5 cells were treated with increasing concentrations of chloroquine (0.04–1.25 µg/mL). The viral curve (VC) corresponds to MRC-5 cells infected with HCoV-OC43 without chloroquine. For all data, n ≥ 3 (biological replicates). Data are representative of three separate experimental runs.

Article Snippet: HCT-8 cells were used for viral replication of HCoV-OC43 (VR-1558), as recommended by the ATCC.

Techniques: Activity Assay, Infection

Journal: Molecules

Article Title: Antiviral Activity of Essential Oil from Populus balsamifera L. Buds and Its Major Compounds Against Betacoronavirus HCoV-OC43 Using a Sensitive Cytoprotection Assay

doi: 10.3390/molecules31091496

Figure Lengend Snippet: Determination of half maximum effective concentration (EC 50 ) of chloroquine against MRC-5 infection with HCoV-OC43. The non-linear sigmoidal regression was obtained using AUC c calculated from chloroquine. Growing concentrations ranged from 0.04 to 1.5 µg/mL. The EC 50 values (cytoprotection against virus-induced cell death) were derived using a non-linear sigmoidal regression equation. For all data, n ≥ 3 (biological replicates). Data are representative of three separate experimental runs.

Article Snippet: HCT-8 cells were used for viral replication of HCoV-OC43 (VR-1558), as recommended by the ATCC.

Techniques: Concentration Assay, Infection, Virus, Derivative Assay

Journal: Molecules

Article Title: Antiviral Activity of Essential Oil from Populus balsamifera L. Buds and Its Major Compounds Against Betacoronavirus HCoV-OC43 Using a Sensitive Cytoprotection Assay

doi: 10.3390/molecules31091496

Figure Lengend Snippet: Antiviral activity of P. balsamifera essential oil. MRC-5 cells were infected with growing concentrations of HCoV-OC43 and treated at concentrations ranged from between 0.2 and 14.4 µg/mL of P. balsamifera essential oil. The EC 50 value (cytoprotection against virus-induced cell death) was then calculated from the generated dose–response curves. The control is represented by the viral curve (VC). For all data, n ≥ 3 (biological replicates). Data are representative of three separate experimental runs.

Article Snippet: HCT-8 cells were used for viral replication of HCoV-OC43 (VR-1558), as recommended by the ATCC.

Techniques: Activity Assay, Infection, Virus, Generated, Control

Journal: Molecules

Article Title: Antiviral Activity of Essential Oil from Populus balsamifera L. Buds and Its Major Compounds Against Betacoronavirus HCoV-OC43 Using a Sensitive Cytoprotection Assay

doi: 10.3390/molecules31091496

Figure Lengend Snippet: Antiviral activity of α-bisabolol ( A ) and nerolidol ( B ). MRC-5 cells were infected with growing concentrations of HCoV-OC43 and treated at different concentrations of α-bisabolol or nerolidol. Concentrations ranged between 0.2 and 14.4 µg/mL for both molecules. The EC 50 value (cytoprotection against virus-induced cell death) was then calculated from the generated dose–response curves. The control is represented by the viral curve (VC). For all data, n ≥ 3 (biological replicates). Data are representative of three separate experimental runs.

Article Snippet: HCT-8 cells were used for viral replication of HCoV-OC43 (VR-1558), as recommended by the ATCC.

Techniques: Activity Assay, Infection, Virus, Generated, Control

IFI16 promotes coronavirus infection. ( a) CRISPR-Cas9 technology was used to knock out IFI16 in A549 cells and single cell clones were screened for loss of IFI16 expression using qRT-PCR. Data is representative of two independent experiments. ( b) Immunoblot showing the loss of IFI16 expression in IFI16-KO cell line. ( c) Immunofluorescence assay showing reduced number of HCoV-OC43 N-positive cells upon loss of IFI16. Cells were infected at an MOI of 0.1 and incubated for the indicated length of time before fixation and staining for HCoV-OC43 N protein. Scale bar is 10 um. ( d ) Representative flow cytometry analysis of wildtype (WT) A549 cells and IFI16-KO cells infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 and 0.1 for 12, 24 and 48 h. Data is representative of 3 independent experiments. (e) and (f) Quantification of flow cytometry data in . ( g) Representative flow cytometry analysis of CRISPRi cell lines infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 for 12, 24 and 48 h. data in figure. (h) Quantification of flow cytometry data in . Data is representative of two independent experiments. Statistical significance was assessed by ANOVA. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.

Journal: bioRxiv

Article Title: Proximity proteomics reveals a role for IFI16 during human coronavirus infection

doi: 10.64898/2026.04.22.720112

Figure Lengend Snippet: IFI16 promotes coronavirus infection. ( a) CRISPR-Cas9 technology was used to knock out IFI16 in A549 cells and single cell clones were screened for loss of IFI16 expression using qRT-PCR. Data is representative of two independent experiments. ( b) Immunoblot showing the loss of IFI16 expression in IFI16-KO cell line. ( c) Immunofluorescence assay showing reduced number of HCoV-OC43 N-positive cells upon loss of IFI16. Cells were infected at an MOI of 0.1 and incubated for the indicated length of time before fixation and staining for HCoV-OC43 N protein. Scale bar is 10 um. ( d ) Representative flow cytometry analysis of wildtype (WT) A549 cells and IFI16-KO cells infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 and 0.1 for 12, 24 and 48 h. Data is representative of 3 independent experiments. (e) and (f) Quantification of flow cytometry data in . ( g) Representative flow cytometry analysis of CRISPRi cell lines infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 for 12, 24 and 48 h. data in figure. (h) Quantification of flow cytometry data in . Data is representative of two independent experiments. Statistical significance was assessed by ANOVA. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.

Article Snippet: Cells were washed twice with a permeabilization/wash buffer (0.1% saponin and 0.5% BSA in 1X PBS) and stained with a 1:10000 dilution of anti-HCoV-OC43-N rabbit polyclonal antibody (catalogue no. 40643-T62; Sino Biological) for 30 min at 4°C.

Techniques: Infection, CRISPR, Knock-Out, Single Cell, Clone Assay, Expressing, Quantitative RT-PCR, Western Blot, Immunofluorescence, Incubation, Staining, Flow Cytometry

(a) PROTACs are bifunctional molecules, consisting of a target protein-binding ligand and an E3 ligase ligand attached via a linker. Upon binding of both targets, the E3 ligase catalyses ubiquitination of the target protein, inducing its degradation. (b) Structure of p38α/β-targeting PROTAC NR-7h. The shaded regions indicate the kinase-binding ligand (based on PH-797804) and the CRBN E3 ligase ligand. A549 cells treated with 10 μM NR-7h for 24 h show significant degradation of p38 kinase (c) but no effects on cell viability, as measured by MTS assay (d) . A549 cells infected with OC43 (MOI=0.1) and treated with 10 μM NR-7h showed significant reduction of viral infectivity (e) and viral RNA (f) after 72 h. (g) NR-7h showed greater antiviral efficacy compared to two p38 kinase small molecule inhibitors (NR-7h, IC 50 1.0 nM; PH-797804, IC 50 >10 µM; LY2228820, IC 50 648.4 nM). Graphs show mean ± SEM (n=3); *, p≤0.05; **, p≤0.01; ***, p≤0.001.

Journal: bioRxiv

Article Title: Proof-of-concept of targeted degradation of p38α/β MAPK host-kinase as a potent inhibitor of coronaviruses

doi: 10.64898/2026.04.29.721712

Figure Lengend Snippet: (a) PROTACs are bifunctional molecules, consisting of a target protein-binding ligand and an E3 ligase ligand attached via a linker. Upon binding of both targets, the E3 ligase catalyses ubiquitination of the target protein, inducing its degradation. (b) Structure of p38α/β-targeting PROTAC NR-7h. The shaded regions indicate the kinase-binding ligand (based on PH-797804) and the CRBN E3 ligase ligand. A549 cells treated with 10 μM NR-7h for 24 h show significant degradation of p38 kinase (c) but no effects on cell viability, as measured by MTS assay (d) . A549 cells infected with OC43 (MOI=0.1) and treated with 10 μM NR-7h showed significant reduction of viral infectivity (e) and viral RNA (f) after 72 h. (g) NR-7h showed greater antiviral efficacy compared to two p38 kinase small molecule inhibitors (NR-7h, IC 50 1.0 nM; PH-797804, IC 50 >10 µM; LY2228820, IC 50 648.4 nM). Graphs show mean ± SEM (n=3); *, p≤0.05; **, p≤0.01; ***, p≤0.001.

Article Snippet: Human coronaviruses OC43 (ATCC VR-1558) stocks were grown in HCT-8 cells, and human coronavirus 229E (ATCC VR-740) stocks were grown and titred in MRC-5 cells.

Techniques: Protein Binding, Binding Assay, Ubiquitin Proteomics, MTS Assay, Infection

BHK-21 cells treated with 10 μM NR-7h for 24 h show significant degradation of p38 kinase (a) but no effects on cell viability, as measured by MTS assay (b) . BHK-21 cells infected with OC43 (MOI=0.1) and treated with 10 μM NR-7h showed significant reduction of viral infectivity (c) and viral RNA (d) after 72 h. (e) NR-7h showed greater inhibition in BHK-21 cells compared to two p38 kinase small molecule inhibitors (NR-7h, IC 50 9.9 nM; PH-797804, IC 50 3.5 µM; LY2228820, IC 50 69.3 nM). Graphs show mean ± SEM (n=3); *, p≤0.05; **, p≤0.01; ***, p≤0.001. Graphs show mean ± SEM (n=3); *, p≤0.05.

Journal: bioRxiv

Article Title: Proof-of-concept of targeted degradation of p38α/β MAPK host-kinase as a potent inhibitor of coronaviruses

doi: 10.64898/2026.04.29.721712

Figure Lengend Snippet: BHK-21 cells treated with 10 μM NR-7h for 24 h show significant degradation of p38 kinase (a) but no effects on cell viability, as measured by MTS assay (b) . BHK-21 cells infected with OC43 (MOI=0.1) and treated with 10 μM NR-7h showed significant reduction of viral infectivity (c) and viral RNA (d) after 72 h. (e) NR-7h showed greater inhibition in BHK-21 cells compared to two p38 kinase small molecule inhibitors (NR-7h, IC 50 9.9 nM; PH-797804, IC 50 3.5 µM; LY2228820, IC 50 69.3 nM). Graphs show mean ± SEM (n=3); *, p≤0.05; **, p≤0.01; ***, p≤0.001. Graphs show mean ± SEM (n=3); *, p≤0.05.

Article Snippet: Human coronaviruses OC43 (ATCC VR-1558) stocks were grown in HCT-8 cells, and human coronavirus 229E (ATCC VR-740) stocks were grown and titred in MRC-5 cells.

Techniques: MTS Assay, Infection, Inhibition

(a) BHK-21 cells were infected with OC43, and treated with 10 µM NR-7h at 0, 1, 3, or 6 h post-infection, and quantified 14 h post-infection. (b) Quantification of infection was normalised to DMSO-treated cells. Inhibition of infection was not observed for any of these conditions. Graphs show mean ± SEM (n=3).

Journal: bioRxiv

Article Title: Proof-of-concept of targeted degradation of p38α/β MAPK host-kinase as a potent inhibitor of coronaviruses

doi: 10.64898/2026.04.29.721712

Figure Lengend Snippet: (a) BHK-21 cells were infected with OC43, and treated with 10 µM NR-7h at 0, 1, 3, or 6 h post-infection, and quantified 14 h post-infection. (b) Quantification of infection was normalised to DMSO-treated cells. Inhibition of infection was not observed for any of these conditions. Graphs show mean ± SEM (n=3).

Article Snippet: Human coronaviruses OC43 (ATCC VR-1558) stocks were grown in HCT-8 cells, and human coronavirus 229E (ATCC VR-740) stocks were grown and titred in MRC-5 cells.

Techniques: Infection, Inhibition

Cell viability of A549 cells treated with (a) MG132 or (b) MLN4924, no cytotoxicity was observed up to 10 µM for both inhibitors. A549 cells treated with 10 µM NR-7h and (c) MG132 (5 µM) or (d) MLN4924 (3 µM) showed no degradation of p38 kinase compared to when no inhibitors were present. A549 cells infected with OC43 (MOI=0.1) showed significant reduction in infection 72 h after treatment in presence of NR-7h, but infectivity was recovered in presence of (e) MG132 (5 µM) or (f) MLN4924 (3 µM). Graphs show mean ± SEM (n=3); **, p≤0.01; ***, p≤0.001.

Journal: bioRxiv

Article Title: Proof-of-concept of targeted degradation of p38α/β MAPK host-kinase as a potent inhibitor of coronaviruses

doi: 10.64898/2026.04.29.721712

Figure Lengend Snippet: Cell viability of A549 cells treated with (a) MG132 or (b) MLN4924, no cytotoxicity was observed up to 10 µM for both inhibitors. A549 cells treated with 10 µM NR-7h and (c) MG132 (5 µM) or (d) MLN4924 (3 µM) showed no degradation of p38 kinase compared to when no inhibitors were present. A549 cells infected with OC43 (MOI=0.1) showed significant reduction in infection 72 h after treatment in presence of NR-7h, but infectivity was recovered in presence of (e) MG132 (5 µM) or (f) MLN4924 (3 µM). Graphs show mean ± SEM (n=3); **, p≤0.01; ***, p≤0.001.

Article Snippet: Human coronaviruses OC43 (ATCC VR-1558) stocks were grown in HCT-8 cells, and human coronavirus 229E (ATCC VR-740) stocks were grown and titred in MRC-5 cells.

Techniques: Infection

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