Journal: Diagnostics

Article Title: Evaluation of a Sample-to-Result POCKIT Central SARS-CoV-2 PCR System

doi: 10.3390/diagnostics13132219

Figure Lengend Snippet: Analytical specificity of POCKIT Central SARS-CoV-2 RT-iiPCR assay and the reference TaqPath COVID-19 PCR assay.

Article Snippet: Human coronavirus OC43 , ATCC VR-1558D , Negative , Negative.

Techniques:

Journal: bioRxiv

Article Title: A stable subgenomic reporter coronavirus enables transcriptional profiling of bystander cells

doi: 10.64898/2026.02.27.708290

Figure Lengend Snippet: Reagents for analysing HCoV-OC43. A. Western blot of Mv.1.Lu cells infected with HCoV-OC43 at an MOI of 10 PFU/cell and harvested at the indicated time points, probed with two anti-Nucleocapsid (N) antibodies, derived from Sheep (Sh) polyclonal antiserum from the MRC PPU & CVR Coronavirus toolkit and a commercial Rabbit (Rb) polyclonal antiserum. Host HSP90 is included as a loading control. B . Immunofluorescence microscopy of HCoV-infected A549 cells, stained with Sheep anti-N and anti double-stranded RNA (dsRNA), a marker of RNA replication. C . Immunoprecipitation (IP) of lysates from HCoV-OC43-infected cells (MOI 3, 24 hpi) using Sheep anti-N and probed with Sheep anti-N and anti-Spike (S). Host HSP90 is included as a loading control. D . Immunofluorescence microscopy of HCoV-OC43-infected A549 cells, stained with mouse monoclonal (Mm) or rabbit polyclonal (Rb) anti-S. E . Silver stain (SS) and western blotting (WB) of virions purified by ultracentrifugation through a 30% sucrose cushion. The supernatant (sup) before and after sucrose cushion, and the pellet, containing purified virions, are shown. Viral structural proteins are indicated by red arrowheads and BSA from cell culture medium by a red asterisk. F . Immunofluorescence microscopy of HCoV-OC43-infected A549 cells stained with anti-SARS-CoV M. Scale bars represent 10 µm.

Article Snippet: Membranes were probed with sheep polyclonal anti-Nucleocapsid anti-serum (MRC PPU & CVR Coronavirus Toolkit, Sheep No. DA116, 1:1000) , rabbit anti-Nucleocapsid (40643-T62, Sino Biological, 1:1000), anti-Spike (PAB21478-100, The Native Antigen Company, 1:1000), anti-SARS-CoV Membrane (ABIN1887462, Antibodies Online, 1:200), anti-MHV nucleocapsid J3.3 ( ) (1:200), anti-GAPDH (AM4300, Invitrogen, 1:4000) and anti-HSP90 (MA5-35624, Invitrogen, 1:2000).

Techniques: Western Blot, Infection, Derivative Assay, Control, Immunofluorescence, Microscopy, Staining, Marker, Immunoprecipitation, Silver Staining, Purification, Cell Culture

Journal: bioRxiv

Article Title: A stable subgenomic reporter coronavirus enables transcriptional profiling of bystander cells

doi: 10.64898/2026.02.27.708290

Figure Lengend Snippet: Reagents for analysing HCoV-OC43. A. Western blot of Mv.1.Lu cells infected with HCoV-OC43 at an MOI of 10 PFU/cell and harvested at the indicated time points, probed with two anti-Nucleocapsid (N) antibodies, derived from Sheep (Sh) polyclonal antiserum from the MRC PPU & CVR Coronavirus toolkit and a commercial Rabbit (Rb) polyclonal antiserum. Host HSP90 is included as a loading control. B . Immunofluorescence microscopy of HCoV-infected A549 cells, stained with Sheep anti-N and anti double-stranded RNA (dsRNA), a marker of RNA replication. C . Immunoprecipitation (IP) of lysates from HCoV-OC43-infected cells (MOI 3, 24 hpi) using Sheep anti-N and probed with Sheep anti-N and anti-Spike (S). Host HSP90 is included as a loading control. D . Immunofluorescence microscopy of HCoV-OC43-infected A549 cells, stained with mouse monoclonal (Mm) or rabbit polyclonal (Rb) anti-S. E . Silver stain (SS) and western blotting (WB) of virions purified by ultracentrifugation through a 30% sucrose cushion. The supernatant (sup) before and after sucrose cushion, and the pellet, containing purified virions, are shown. Viral structural proteins are indicated by red arrowheads and BSA from cell culture medium by a red asterisk. F . Immunofluorescence microscopy of HCoV-OC43-infected A549 cells stained with anti-SARS-CoV M. Scale bars represent 10 µm.

Article Snippet: Membranes were probed with sheep polyclonal anti-Nucleocapsid anti-serum (MRC PPU & CVR Coronavirus Toolkit, Sheep No. DA116, 1:1000) , rabbit anti-Nucleocapsid (40643-T62, Sino Biological, 1:1000), anti-Spike (PAB21478-100, The Native Antigen Company, 1:1000), anti-SARS-CoV Membrane (ABIN1887462, Antibodies Online, 1:200), anti-MHV nucleocapsid J3.3 ( ) (1:200), anti-GAPDH (AM4300, Invitrogen, 1:4000) and anti-HSP90 (MA5-35624, Invitrogen, 1:2000).

Techniques: Western Blot, Infection, Derivative Assay, Control, Immunofluorescence, Microscopy, Staining, Marker, Immunoprecipitation, Silver Staining, Purification, Cell Culture

Journal: Mikrochimica Acta

Article Title: An electrochemical immunosensor for the corona virus associated with the Middle East respiratory syndrome using an array of gold nanoparticle-modified carbon electrodes

doi: 10.1007/s00604-019-3345-5

Figure Lengend Snippet: a Optimization of MERS-CoV antibody concentration, b Effect of MERS-CoV immunosensor binding time between antibody and immobilized antigen. c Effect of HCoV immunosensor binding time between antibody and immobilized antigen. n = 3

Article Snippet: Antigen of HCoV (Oc43 N) and antibody for HCoV - Oc43 were purchased from Medix Biochemica (Finland) ( https://www.medixbiochemica.com/en/ ).

Techniques: Concentration Assay, Binding Assay

Journal: Mikrochimica Acta

Article Title: An electrochemical immunosensor for the corona virus associated with the Middle East respiratory syndrome using an array of gold nanoparticle-modified carbon electrodes

doi: 10.1007/s00604-019-3345-5

Figure Lengend Snippet: a SWV before and after the competition step of MERS-CoV immunosensor and b SWV before and after the competition step of HCoV immunosensor. The calibration plots were based on the % change of the SWV peak currents (I o -I)/I o %, where I o is current measured at the antigen-modified electrodes after blocking with 1% BSA and I is the current measured after incubation with the sample mixed with the antibody, versus the logarithm of the different concentrations of MERS-CoV ( c ) or HCOV ( d ) antigens. n = 3, the current of the calibration plot was acquired at −0.05 V

Article Snippet: Antigen of HCoV (Oc43 N) and antibody for HCoV - Oc43 were purchased from Medix Biochemica (Finland) ( https://www.medixbiochemica.com/en/ ).

Techniques: Modification, Blocking Assay, Incubation

Journal: Mikrochimica Acta

Article Title: An electrochemical immunosensor for the corona virus associated with the Middle East respiratory syndrome using an array of gold nanoparticle-modified carbon electrodes

doi: 10.1007/s00604-019-3345-5

Figure Lengend Snippet: a MERS-CoV immunosensor response against 10 ng.mL −1 of MERS-CoV, Flu A, Flu B and control electrode. b HCoV immunosensor response against 10 ng.mL −1 of HCoV, Flu A, Flu B and control. n = 3

Article Snippet: Antigen of HCoV (Oc43 N) and antibody for HCoV - Oc43 were purchased from Medix Biochemica (Finland) ( https://www.medixbiochemica.com/en/ ).

Techniques: Control

Journal: Mikrochimica Acta

Article Title: An electrochemical immunosensor for the corona virus associated with the Middle East respiratory syndrome using an array of gold nanoparticle-modified carbon electrodes

doi: 10.1007/s00604-019-3345-5

Figure Lengend Snippet: Application of MERS-CoV and HCoV immunosensor in spiked nasal samples. n = 3

Article Snippet: Antigen of HCoV (Oc43 N) and antibody for HCoV - Oc43 were purchased from Medix Biochemica (Finland) ( https://www.medixbiochemica.com/en/ ).

Techniques:

Journal: Cell

Article Title: Genome-Scale Identification of SARS-CoV-2 and Pan-coronavirus Host Factor Networks

doi: 10.1016/j.cell.2020.12.006

Figure Lengend Snippet: Genome-wide CRISPR Screens Identify Host Factors Required for SARS-CoV-2 Infection (A) Genome-wide CRISPR screening workflow. Cas9-expressing Huh-7.5 cells are transduced with the Brunello genome-wide CRISPR library, selected with puromycin, and infected with SARS-CoV-2 or one of three seasonal CoVs (HCoV-OC43, HCoV-NL63, or HCoV-229E). Surviving cells and mock controls are then harvested, and sgRNA abundance is determined using next-generation sequencing. (B) Bubble plot of data from SARS-CoV-2 screens at 37°C. Red lines denote z = ±2. (C) Bubble plot of data from SARS-CoV-2 screens at 33°C. Red lines denote z = ±2. (D) Scatterplot comparing Z scores from (B) and (C) for SARS-CoV-2 screens at 37°C and 33°C, respectively. (E) Subset of significantly enriched genes from SARS-CoV-2 screens at 37°C and 33°C.

Article Snippet: HCoV-OC43 , ZeptoMetrix , Cat#0810024CF.

Techniques: Genome Wide, CRISPR, Infection, Expressing, Transduction, Next-Generation Sequencing

Journal: Cell

Article Title: Genome-Scale Identification of SARS-CoV-2 and Pan-coronavirus Host Factor Networks

doi: 10.1016/j.cell.2020.12.006

Figure Lengend Snippet: Analysis of Established and Putative CoV Host Factors and Gene-wise Fitness Scores for SARS-CoV-2 and 3 Seasonal CoVs, Related to and (A) Heatmap of z-scores for known and putative coronavirus host factors. (B-D) Genewise fitness beta scatterplots comparing SARS-CoV-2 at 37°C versus mock (B), SARS-CoV-2 at 33°C versus mock (C), and SARS-CoV-2 at 33°C versus SARS-CoV-2 at 37°C (D). Non-targeting controls and essential control genes are highlighted in blue and red, respectively. (E) HCoV-OC43 versus mock infected. (F) HCoV-NL63 versus mock infected. (G) HCoV-229E versus mock infected.

Article Snippet: HCoV-OC43 , ZeptoMetrix , Cat#0810024CF.

Techniques: Control, Infection

Journal: Cell

Article Title: Genome-Scale Identification of SARS-CoV-2 and Pan-coronavirus Host Factor Networks

doi: 10.1016/j.cell.2020.12.006

Figure Lengend Snippet: Parallel Genome-wide CRISPR Screening against Multiple HCoVs Uncovers Host Factors and Pathways with Pan-CoV and Virus-Specific Functional Roles (A) Bubble plot of data from HCoV-OC43 screens at 33°C. Red lines denote z = ±2. (B) Bubble plot of data from HCoV-NL63 screens at 33°C. Red lines denote z = ±2. (C) Bubble plot of data from HCoV-229E screens at 33°C. Red lines denote z = ±2. (D) UpSet plot showing enriched hits overlapping in screens across all four viruses. Select genes for enriched sgRNAs are indicated.

Article Snippet: HCoV-OC43 , ZeptoMetrix , Cat#0810024CF.

Techniques: Genome Wide, CRISPR, Virus, Functional Assay

Journal: Cell

Article Title: Genome-Scale Identification of SARS-CoV-2 and Pan-coronavirus Host Factor Networks

doi: 10.1016/j.cell.2020.12.006

Figure Lengend Snippet: Expanded CoV-Specific Networks, Related to Figure 3 (A–D) Network diagram of all coronavirus screen hits and the next 100 adjacent interactors, shown in gray. Broad functional categories of highly interconnected gene neighborhoods are indicated. Diagrams are for SARS-CoV-2 (A), HCoV-OC43 (B), HCoV-NL63 (C) and HCoV-229E (D).

Article Snippet: HCoV-OC43 , ZeptoMetrix , Cat#0810024CF.

Techniques: Functional Assay

Journal: Cell

Article Title: Genome-Scale Identification of SARS-CoV-2 and Pan-coronavirus Host Factor Networks

doi: 10.1016/j.cell.2020.12.006

Figure Lengend Snippet: Validation of High-Confidence Coronaviridae Host Factors (A) Candidate validation in Huh-7.5 cells with SARS-CoV-2 infection at 33°C. (B) Candidate validation in Huh-7.5 cells with HCoV-OC43 infection at 33°C. (C) Candidate validation in Huh-7.5 cells with HCoV-NL63 at 33°C. (D) Candidate validation in Huh-7.5 cells with HCoV-229E at 37°C. (E) Heatmap representation of data from (A)–(D). The SARS-CoV-2 and HCoV-NL-63 receptor ( ACE2 ) and the HCoV-229E receptor ( ANPEP ) are shown separately.

Article Snippet: HCoV-OC43 , ZeptoMetrix , Cat#0810024CF.

Techniques: Infection

Journal: Cell

Article Title: Genome-Scale Identification of SARS-CoV-2 and Pan-coronavirus Host Factor Networks

doi: 10.1016/j.cell.2020.12.006

Figure Lengend Snippet: A Majority of Functional CoV Host Factors Are Expressed in the Airway, Related to , , and (A–D) scRNaseq expression dotplot diagrams from select cells in the airway of the average expression and the percent of cells expressing coronavirus host factors for SARS-CoV-2 (A), HCoV-OC43 (B), HCoV-NL63 (C) and HCoV-229E (D). Rows for each diagram are ordered top to bottom from highest to lowest z-score. Data are from Chua et al. (2020) .

Article Snippet: HCoV-OC43 , ZeptoMetrix , Cat#0810024CF.

Techniques: Functional Assay, Expressing

Journal: Cell

Article Title: Genome-Scale Identification of SARS-CoV-2 and Pan-coronavirus Host Factor Networks

doi: 10.1016/j.cell.2020.12.006

Figure Lengend Snippet:

Article Snippet: HCoV-OC43 , ZeptoMetrix , Cat#0810024CF.

Techniques: Virus, Recombinant, SYBR Green Assay, Luciferase, DNA Purification, Plasmid Preparation, CRISPR, Software, Genome Wide, Knock-Out, Inverted Microscopy

Journal: Cell Reports Medicine

Article Title: Decay of Fc-dependent antibody functions after mild to moderate COVID-19

doi: 10.1016/j.xcrm.2021.100296

Figure Lengend Snippet: Dynamics of dimeric FcγR-binding antibodies against HCoV S antigens in COVID-19 convalescent individuals (A) Best-fit decay slopes of IgG and dimeric FcγR-binding antibodies against S from HCoV strains OC43, HKU1, 229E, and NL63. The responses at time point 1 for each parameter are set to 100%, and the %change over time is shown. (B–E) Kinetics of dimeric FcγRIIIa (V158) and FcγRIIa (H131) binding antibodies against S, S1, or S2 from HCoV strains (B) OC43, (C) HKU1, (D) 229E, and (E) NL63 over time in COVID-19 convalescent individuals (N = 53) measured using the bead-based multiplex assay. The best-fit decay slopes (red lines) and estimated half-lives ( t 1/2 ) are indicated for COVID-19 convalescent individuals. Uninfected controls (N = 33) are shown in open circles, with the median and 90% percentile responses presented as thick and thin dashed lines, respectively. The limit of detection is shown as the shaded area. See also Figure S6 .

Article Snippet: HCoV-OC43 Spike , Sino Biological , 40607-V08B.

Techniques: Binding Assay, Multiplex Assay

Journal: Cell Reports Medicine

Article Title: Decay of Fc-dependent antibody functions after mild to moderate COVID-19

doi: 10.1016/j.xcrm.2021.100296

Figure Lengend Snippet:

Article Snippet: HCoV-OC43 Spike , Sino Biological , 40607-V08B.

Techniques: Infection, Recombinant, Enzyme-linked Immunosorbent Assay, Luciferase, Reporter Gene Assay, Plasmid Preparation, Software

Journal: Heliyon

Article Title: Irradiation of UVC LED at 277 nm inactivates coronaviruses in association to photodegradation of spike protein

doi: 10.1016/j.heliyon.2022.e11132

Figure Lengend Snippet: Key Resources Table

Article Snippet: Anti-Coronavirus OC43 Spike Protein antibody , Cusabio , CSB-PA336163EA01HIY.

Techniques: Virus, Recombinant, Purification, Silver Staining, Software