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anti nlrp3 ag 20b 0014 antibody  (Adipogen)

 
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    Adipogen anti nlrp3 ag 20b 0014 antibody
    Anti Nlrp3 Ag 20b 0014 Antibody, supplied by Adipogen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nlrp3/pm42242598-57-1-7?v=Adipogen
    Average 86 stars, based on 1 article reviews
    anti nlrp3 ag 20b 0014 antibody - by Bioz Stars, 2026-07
    86/100 stars

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    Image Search Results


    BMDMs from WT mice were treated with either media alone, lipopolysaccharide, LPS alone, LPS plus nigericin (LPS + NIG), or LPS + NIG plus OLT1177 (NLRP3 inhibitor). Nigericin (10 μM) was added to BMDMs after 3.5 hours of stimulation and cytokines were measured in the culture supernatants at 4h. IL-1β (A), CCL3 (B), and MPO (C) were measured in the culture supernatants by the R&D Duo set ELISA kit. Data are presented as mean ± SEM from three independent biological replicates (BMDMs derived from three independent mice). ****P< .0001.

    Journal: bioRxiv

    Article Title: OLT1177 (Dapansutrile) inhibits Gasdermin D-dependent IL-1β Release and Pyroptotic Cell Death in Bone Marrow-derived Macrophages

    doi: 10.64898/2026.05.31.729061

    Figure Lengend Snippet: BMDMs from WT mice were treated with either media alone, lipopolysaccharide, LPS alone, LPS plus nigericin (LPS + NIG), or LPS + NIG plus OLT1177 (NLRP3 inhibitor). Nigericin (10 μM) was added to BMDMs after 3.5 hours of stimulation and cytokines were measured in the culture supernatants at 4h. IL-1β (A), CCL3 (B), and MPO (C) were measured in the culture supernatants by the R&D Duo set ELISA kit. Data are presented as mean ± SEM from three independent biological replicates (BMDMs derived from three independent mice). ****P< .0001.

    Article Snippet: After 3.5 hours, the NLRP3 inflammasome formation was induced with 10 μM nigericin (InvivoGen, San Diego, CA) for 30 minutes.

    Techniques: Enzyme-linked Immunosorbent Assay, Derivative Assay

    A) BMDMs were treated with lipopolysaccharide, LPS (1μg/mL) and LPS plus nigercin (NIG). Representative western blots show OLT1177 inhibits GSDMD cleavage to GSDMD-N in a dose-dependent manner. (B, C) Densitometric analysis of the GSDMD-N and IL-1β bands from . All concentrations of OLT1177 significantly reduce both GSDMD-N and IL-1β in a dose-dependent manner. D) Western blot shows NLRP3 and β-actin expression in BMDMs. Data are representative of three independent biological replicates (BMDMs derived from three independent mice). ****P< 0.0001.

    Journal: bioRxiv

    Article Title: OLT1177 (Dapansutrile) inhibits Gasdermin D-dependent IL-1β Release and Pyroptotic Cell Death in Bone Marrow-derived Macrophages

    doi: 10.64898/2026.05.31.729061

    Figure Lengend Snippet: A) BMDMs were treated with lipopolysaccharide, LPS (1μg/mL) and LPS plus nigercin (NIG). Representative western blots show OLT1177 inhibits GSDMD cleavage to GSDMD-N in a dose-dependent manner. (B, C) Densitometric analysis of the GSDMD-N and IL-1β bands from . All concentrations of OLT1177 significantly reduce both GSDMD-N and IL-1β in a dose-dependent manner. D) Western blot shows NLRP3 and β-actin expression in BMDMs. Data are representative of three independent biological replicates (BMDMs derived from three independent mice). ****P< 0.0001.

    Article Snippet: After 3.5 hours, the NLRP3 inflammasome formation was induced with 10 μM nigericin (InvivoGen, San Diego, CA) for 30 minutes.

    Techniques: Western Blot, Expressing, Derivative Assay

    LPS induces cell activation and expression of the NLRP3 gene. Nigericin is a polyether antibiotic and acts as a potassium ionophore, causing NLRP3 inflammasome activation. Caspase-1 cleaves pro-IL-1β and GSDMD to IL-1β and GSDM-N, respectively. Mature IL-1β then exits the cells through the GSDMD-N pores.

    Journal: bioRxiv

    Article Title: OLT1177 (Dapansutrile) inhibits Gasdermin D-dependent IL-1β Release and Pyroptotic Cell Death in Bone Marrow-derived Macrophages

    doi: 10.64898/2026.05.31.729061

    Figure Lengend Snippet: LPS induces cell activation and expression of the NLRP3 gene. Nigericin is a polyether antibiotic and acts as a potassium ionophore, causing NLRP3 inflammasome activation. Caspase-1 cleaves pro-IL-1β and GSDMD to IL-1β and GSDM-N, respectively. Mature IL-1β then exits the cells through the GSDMD-N pores.

    Article Snippet: After 3.5 hours, the NLRP3 inflammasome formation was induced with 10 μM nigericin (InvivoGen, San Diego, CA) for 30 minutes.

    Techniques: Activation Assay, Expressing