Journal: Scientific Reports
Article Title: Novel somatic variants involved in biochemical activity of pure growth hormone-secreting pituitary adenoma without GNAS variant
doi: 10.1038/s41598-021-95829-3
Figure Lengend Snippet: Variant profiles of the validation cohort. ( a ) Analysis pipeline used for targeted resequencing data (drawn in Microsoft 365 Powerpoint). ( b ) Variant profiles of validation cohort. Genes were sorted in descending order of frequency (created in Microsoft 365 Excel). ( c ) Representative images of Sanger sequencing of DARS , BCHE , and NGDN . Left and right panels represent the genotype of the tissue and blood, respectively (created in Snap Gene Viewer; https://www.snapgene.com/snapgene-viewer/ ).
Article Snippet: pcDNA3.1-FLAG- BCHE , DARS , NGDN , or UNC5B wild-type (WT) was purchased from Origene (Rockville, MD, USA), and each mutant plasmid of target genes was generated with Site-Directed Mutagenesis Kits according to the manufacturer’s protocol (Thermo Fisher Scientific).
Techniques: Variant Assay, Biomarker Discovery, Sequencing