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Variant profiles of the validation cohort. ( a ) Analysis pipeline used for targeted resequencing data (drawn in Microsoft 365 Powerpoint). ( b ) Variant profiles of validation cohort. Genes were sorted in descending order of frequency (created in Microsoft 365 Excel). ( c ) Representative images of Sanger sequencing of <t>DARS</t> <t>,</t> <t>BCHE</t> , and NGDN . Left and right panels represent the genotype of the tissue and blood, respectively (created in Snap Gene Viewer; https://www.snapgene.com/snapgene-viewer/ ).
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Variant profiles of the validation cohort. ( a ) Analysis pipeline used for targeted resequencing data (drawn in Microsoft 365 Powerpoint). ( b ) Variant profiles of validation cohort. Genes were sorted in descending order of frequency (created in Microsoft 365 Excel). ( c ) Representative images of Sanger sequencing of <t>DARS</t> <t>,</t> <t>BCHE</t> , and NGDN . Left and right panels represent the genotype of the tissue and blood, respectively (created in Snap Gene Viewer; https://www.snapgene.com/snapgene-viewer/ ).
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Variant profiles of the validation cohort. ( a ) Analysis pipeline used for targeted resequencing data (drawn in Microsoft 365 Powerpoint). ( b ) Variant profiles of validation cohort. Genes were sorted in descending order of frequency (created in Microsoft 365 Excel). ( c ) Representative images of Sanger sequencing of <t>DARS</t> <t>,</t> <t>BCHE</t> , and NGDN . Left and right panels represent the genotype of the tissue and blood, respectively (created in Snap Gene Viewer; https://www.snapgene.com/snapgene-viewer/ ).
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Variant profiles of the validation cohort. ( a ) Analysis pipeline used for targeted resequencing data (drawn in Microsoft 365 Powerpoint). ( b ) Variant profiles of validation cohort. Genes were sorted in descending order of frequency (created in Microsoft 365 Excel). ( c ) Representative images of Sanger sequencing of <t>DARS</t> <t>,</t> <t>BCHE</t> , and NGDN . Left and right panels represent the genotype of the tissue and blood, respectively (created in Snap Gene Viewer; https://www.snapgene.com/snapgene-viewer/ ).
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Variant profiles of the validation cohort. ( a ) Analysis pipeline used for targeted resequencing data (drawn in Microsoft 365 Powerpoint). ( b ) Variant profiles of validation cohort. Genes were sorted in descending order of frequency (created in Microsoft 365 Excel). ( c ) Representative images of Sanger sequencing of <t>DARS</t> <t>,</t> <t>BCHE</t> , and NGDN . Left and right panels represent the genotype of the tissue and blood, respectively (created in Snap Gene Viewer; https://www.snapgene.com/snapgene-viewer/ ).
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Variant profiles of the validation cohort. ( a ) Analysis pipeline used for targeted resequencing data (drawn in Microsoft 365 Powerpoint). ( b ) Variant profiles of validation cohort. Genes were sorted in descending order of frequency (created in Microsoft 365 Excel). ( c ) Representative images of Sanger sequencing of DARS , BCHE , and NGDN . Left and right panels represent the genotype of the tissue and blood, respectively (created in Snap Gene Viewer; https://www.snapgene.com/snapgene-viewer/ ).

Journal: Scientific Reports

Article Title: Novel somatic variants involved in biochemical activity of pure growth hormone-secreting pituitary adenoma without GNAS variant

doi: 10.1038/s41598-021-95829-3

Figure Lengend Snippet: Variant profiles of the validation cohort. ( a ) Analysis pipeline used for targeted resequencing data (drawn in Microsoft 365 Powerpoint). ( b ) Variant profiles of validation cohort. Genes were sorted in descending order of frequency (created in Microsoft 365 Excel). ( c ) Representative images of Sanger sequencing of DARS , BCHE , and NGDN . Left and right panels represent the genotype of the tissue and blood, respectively (created in Snap Gene Viewer; https://www.snapgene.com/snapgene-viewer/ ).

Article Snippet: pcDNA3.1-FLAG- BCHE , DARS , NGDN , or UNC5B wild-type (WT) was purchased from Origene (Rockville, MD, USA), and each mutant plasmid of target genes was generated with Site-Directed Mutagenesis Kits according to the manufacturer’s protocol (Thermo Fisher Scientific).

Techniques: Variant Assay, Biomarker Discovery, Sequencing

Clinical characteristics of patients with somatic variants in growth hormone (GH)-secreting pituitary adenoma.

Journal: Scientific Reports

Article Title: Novel somatic variants involved in biochemical activity of pure growth hormone-secreting pituitary adenoma without GNAS variant

doi: 10.1038/s41598-021-95829-3

Figure Lengend Snippet: Clinical characteristics of patients with somatic variants in growth hormone (GH)-secreting pituitary adenoma.

Article Snippet: pcDNA3.1-FLAG- BCHE , DARS , NGDN , or UNC5B wild-type (WT) was purchased from Origene (Rockville, MD, USA), and each mutant plasmid of target genes was generated with Site-Directed Mutagenesis Kits according to the manufacturer’s protocol (Thermo Fisher Scientific).

Techniques: Immunohistochemical staining, Staining