napi iib (Proteintech)
Structured Review
Napi Iib, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/napi-iib/SLC34A2+Antibody/10__1113_slash_jp280345-122-27-44
Average 91 stars, based on 4 article reviews
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Blocking Assay:Article Title: 1,25(OH) 2 vitamin D 3 stimulates active phosphate transport but not paracellular phosphate absorption in mouse intestine Article Snippet: Samples of BBM (20 g) or homogenates (40 g) were separated on SDS/PAGE gels and transferred to PVDF membranes (Millipore) by standard procedures. .. Upon blocking with 5% milk powder in TBS (30 minutes/room temperature), membranes were first incubated (overnight/4°C) with primary antibodies against NaPi-IIa (1: 3000; (Custer et al., 1994), Incubation:Article Title: 1,25(OH) 2 vitamin D 3 stimulates active phosphate transport but not paracellular phosphate absorption in mouse intestine Article Snippet: Samples of BBM (20 g) or homogenates (40 g) were separated on SDS/PAGE gels and transferred to PVDF membranes (Millipore) by standard procedures. .. Upon blocking with 5% milk powder in TBS (30 minutes/room temperature), membranes were first incubated (overnight/4°C) with primary antibodies against NaPi-IIa (1: 3000; (Custer et al., 1994), Article Title: SLC34A2 promotes neuroblastoma cell stemness via enhancement of miR-25/Gsk3β-mediated activation of Wnt/β-catenin signaling. Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense nitrocellulose membrane (Promega, Madison, WI, USA) and incubated with the primary Article Title: SLC 34A2 promotes neuroblastoma cell stemness via enhancement of miR‐25/Gsk3β‐mediated activation of Wnt/β‐catenin signaling Article Snippet: Protein concentration was measured using a BCA Protein Assay Kit (KeyGEN BioTECH). .. Twenty micrograms of protein extract was separated by 10% SDS/PAGE, then transferred to nitrocellulose membrane (Promega, Madison, WI, USA) and incubated with the primary Article Title: MAPK signaling pathway participates in the regulation of intestinal phosphorus and calcium absorption in broiler chickens via 1,25-dihydroxyvitamin D 3 Article Snippet: .. The PVDF membrane was then blocked with 5% nonfat powdered milk in Tris-buffered saline supplemented with tween-20 ( TBST ) at 37°C for 1 h. Subsequently, the membranes were incubated overnight at 4°C with primary Western Blot:Article Title: Effects of EOS789, a novel pan-phosphate transporter inhibitor, on phosphate metabolism : Comparison with a conventional phosphate binder. Article Snippet: Horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse IgG was used as a secondary antibody (Jackson Immuno Research Laboratories, Inc, West Grove, PA, USA). .. Antibody dilutions used for immunoblotting were as follows : NaPi-2a (1 : 25,000), Membrane:Article Title: SLC34A2 promotes neuroblastoma cell stemness via enhancement of miR-25/Gsk3β-mediated activation of Wnt/β-catenin signaling. Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense nitrocellulose membrane (Promega, Madison, WI, USA) and incubated with the primary Article Title: SLC 34A2 promotes neuroblastoma cell stemness via enhancement of miR‐25/Gsk3β‐mediated activation of Wnt/β‐catenin signaling Article Snippet: Protein concentration was measured using a BCA Protein Assay Kit (KeyGEN BioTECH). .. Twenty micrograms of protein extract was separated by 10% SDS/PAGE, then transferred to nitrocellulose membrane (Promega, Madison, WI, USA) and incubated with the primary Article Title: MAPK signaling pathway participates in the regulation of intestinal phosphorus and calcium absorption in broiler chickens via 1,25-dihydroxyvitamin D 3 Article Snippet: .. The PVDF membrane was then blocked with 5% nonfat powdered milk in Tris-buffered saline supplemented with tween-20 ( TBST ) at 37°C for 1 h. Subsequently, the membranes were incubated overnight at 4°C with primary Saline:Article Title: MAPK signaling pathway participates in the regulation of intestinal phosphorus and calcium absorption in broiler chickens via 1,25-dihydroxyvitamin D 3 Article Snippet: .. The PVDF membrane was then blocked with 5% nonfat powdered milk in Tris-buffered saline supplemented with tween-20 ( TBST ) at 37°C for 1 h. Subsequently, the membranes were incubated overnight at 4°C with primary |
