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Proteintech napi iib
Napi Iib, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/napi-iib/SLC34A2+Antibody/10__1113_slash_jp280345-122-27-44
Average 91 stars, based on 4 article reviews
napi iib - by Bioz Stars, 2026-09
91/100 stars

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Related Articles

Blocking Assay:

Article Title: 1,25(OH) 2 vitamin D 3 stimulates active phosphate transport but not paracellular phosphate absorption in mouse intestine
Article Snippet: Samples of BBM (20 g) or homogenates (40 g) were separated on SDS/PAGE gels and transferred to PVDF membranes (Millipore) by standard procedures. .. Upon blocking with 5% milk powder in TBS (30 minutes/room temperature), membranes were first incubated (overnight/4°C) with primary antibodies against NaPi-IIa (1: 3000; (Custer et al., 1994), NaPi-IIb (1: 3000; (Hilfiker et al., 1998), NaPi-IIc (1: 3000; (Nowik et al., 2008), Cyp24a1 (1: 1000; Proteintech: ≠21582-1-AP), VDR (1: 500; Santa Cruz: ≠sc-13133), β-actin (1: 10000; Sigma: ≠ A5316) and β-tubulin (1: 20000; Sigma ≠T4026) and then with the appropriate HRP-conjugated secondary antibody (GE Healthcare) (2 hours/room temperature). .. Upon exposure to HRP substrate (Millipore), chemiluminescence signals were detected with the LAS-4000 luminescent image analyser (Fujifilm) and further quantified (ImageJ).

Incubation:

Article Title: 1,25(OH) 2 vitamin D 3 stimulates active phosphate transport but not paracellular phosphate absorption in mouse intestine
Article Snippet: Samples of BBM (20 g) or homogenates (40 g) were separated on SDS/PAGE gels and transferred to PVDF membranes (Millipore) by standard procedures. .. Upon blocking with 5% milk powder in TBS (30 minutes/room temperature), membranes were first incubated (overnight/4°C) with primary antibodies against NaPi-IIa (1: 3000; (Custer et al., 1994), NaPi-IIb (1: 3000; (Hilfiker et al., 1998), NaPi-IIc (1: 3000; (Nowik et al., 2008), Cyp24a1 (1: 1000; Proteintech: ≠21582-1-AP), VDR (1: 500; Santa Cruz: ≠sc-13133), β-actin (1: 10000; Sigma: ≠ A5316) and β-tubulin (1: 20000; Sigma ≠T4026) and then with the appropriate HRP-conjugated secondary antibody (GE Healthcare) (2 hours/room temperature). .. Upon exposure to HRP substrate (Millipore), chemiluminescence signals were detected with the LAS-4000 luminescent image analyser (Fujifilm) and further quantified (ImageJ).

Article Title: SLC34A2 promotes neuroblastoma cell stemness via enhancement of miR-25/Gsk3β-mediated activation of Wnt/β-catenin signaling.
Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense nitrocellulose membrane (Promega, Madison, WI, USA) and incubated with the primary antibodies against SLC34A2 (cat. no. 21295-1-AP), Nanog (cat. no. 14295-1- AP), aldehyde dehydrogenase 1 (ALDH1) A1 (cat. no. 15910-1-AP), and b-actin (cat. no. 60008-1-Ig) were purchased from Proteintech (Wuhan, Hubei, P.R.C). .. Primary antibodies against Gsk3b (ab93926), Wnt3a (ab28472) and b-catenin (ab32572) were purchased from Abcam (Cambridge, MA, USA).

Article Title: SLC 34A2 promotes neuroblastoma cell stemness via enhancement of miR‐25/Gsk3β‐mediated activation of Wnt/β‐catenin signaling
Article Snippet: Protein concentration was measured using a BCA Protein Assay Kit (KeyGEN BioTECH). .. Twenty micrograms of protein extract was separated by 10% SDS/PAGE, then transferred to nitrocellulose membrane (Promega, Madison, WI, USA) and incubated with the primary antibodies against SLC34A2 (cat. no. 21295‐1‐AP), Nanog (cat. no. 14295‐1‐AP), aldehyde dehydrogenase 1 (ALDH1) A1 (cat. no. 15910‐1‐AP), and β‐actin (cat. no. 60008‐1‐Ig) were purchased from Proteintech (Wuhan, Hubei, P.R.C). .. Primary antibodies against Gsk3β (ab93926), Wnt3a (ab28472) and β‐catenin (ab32572) were purchased from Abcam (Cambridge, MA, USA).

Article Title: MAPK signaling pathway participates in the regulation of intestinal phosphorus and calcium absorption in broiler chickens via 1,25-dihydroxyvitamin D 3
Article Snippet: .. The PVDF membrane was then blocked with 5% nonfat powdered milk in Tris-buffered saline supplemented with tween-20 ( TBST ) at 37°C for 1 h. Subsequently, the membranes were incubated overnight at 4°C with primary antibodies against GAPDH (1:10,000, 60004-1-Ig, Proteintech, Wuhan, China), NaPi-IIb (1:1,000, A9460, ABclonal, Wuhan, China), CaBP-D28k (1:5,000, 14479-1-AP, Proteintech, Wuhan, China), p-ERK (1:1,000, 9101S, Cell Signaling Technology, Boston, USA), t-ERK (1:8,000, 11257-1-AP-50, Proteintech, Wuhan, China), p-p38MAPK (1:1,000, 9216S, Cell Signaling Technology, Boston, USA), and t-p38MAPK (1:1,000, 14064-1-AP, Proteintech, Wuhan, China). ..

Western Blot:

Article Title: Effects of EOS789, a novel pan-phosphate transporter inhibitor, on phosphate metabolism : Comparison with a conventional phosphate binder.
Article Snippet: Horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse IgG was used as a secondary antibody (Jackson Immuno Research Laboratories, Inc, West Grove, PA, USA). .. Antibody dilutions used for immunoblotting were as follows : NaPi-2a (1 : 25,000), NaPi-2b (1 : 2,000), NaPi-2c (1 : 4,000), Pit-1 (Genetex inc, CA, USA, 1 : 1,500), Xpr1 (PROTEINTECH, 1 : 2000), NHE3 (Millipore, 1 : 6,000), and actin (1 : 100,000). .. Antibody dilutions used for immunofluorescence staining were as follows : NaPi-2a (1 : 500), NaPi-2b, NaPi-2c (1 : 300), NHE3 (Millipore, 1 : 500), and villin (Millipore, 1 : 200).

Membrane:

Article Title: SLC34A2 promotes neuroblastoma cell stemness via enhancement of miR-25/Gsk3β-mediated activation of Wnt/β-catenin signaling.
Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense nitrocellulose membrane (Promega, Madison, WI, USA) and incubated with the primary antibodies against SLC34A2 (cat. no. 21295-1-AP), Nanog (cat. no. 14295-1- AP), aldehyde dehydrogenase 1 (ALDH1) A1 (cat. no. 15910-1-AP), and b-actin (cat. no. 60008-1-Ig) were purchased from Proteintech (Wuhan, Hubei, P.R.C). .. Primary antibodies against Gsk3b (ab93926), Wnt3a (ab28472) and b-catenin (ab32572) were purchased from Abcam (Cambridge, MA, USA).

Article Title: SLC 34A2 promotes neuroblastoma cell stemness via enhancement of miR‐25/Gsk3β‐mediated activation of Wnt/β‐catenin signaling
Article Snippet: Protein concentration was measured using a BCA Protein Assay Kit (KeyGEN BioTECH). .. Twenty micrograms of protein extract was separated by 10% SDS/PAGE, then transferred to nitrocellulose membrane (Promega, Madison, WI, USA) and incubated with the primary antibodies against SLC34A2 (cat. no. 21295‐1‐AP), Nanog (cat. no. 14295‐1‐AP), aldehyde dehydrogenase 1 (ALDH1) A1 (cat. no. 15910‐1‐AP), and β‐actin (cat. no. 60008‐1‐Ig) were purchased from Proteintech (Wuhan, Hubei, P.R.C). .. Primary antibodies against Gsk3β (ab93926), Wnt3a (ab28472) and β‐catenin (ab32572) were purchased from Abcam (Cambridge, MA, USA).

Article Title: MAPK signaling pathway participates in the regulation of intestinal phosphorus and calcium absorption in broiler chickens via 1,25-dihydroxyvitamin D 3
Article Snippet: .. The PVDF membrane was then blocked with 5% nonfat powdered milk in Tris-buffered saline supplemented with tween-20 ( TBST ) at 37°C for 1 h. Subsequently, the membranes were incubated overnight at 4°C with primary antibodies against GAPDH (1:10,000, 60004-1-Ig, Proteintech, Wuhan, China), NaPi-IIb (1:1,000, A9460, ABclonal, Wuhan, China), CaBP-D28k (1:5,000, 14479-1-AP, Proteintech, Wuhan, China), p-ERK (1:1,000, 9101S, Cell Signaling Technology, Boston, USA), t-ERK (1:8,000, 11257-1-AP-50, Proteintech, Wuhan, China), p-p38MAPK (1:1,000, 9216S, Cell Signaling Technology, Boston, USA), and t-p38MAPK (1:1,000, 14064-1-AP, Proteintech, Wuhan, China). ..

Saline:

Article Title: MAPK signaling pathway participates in the regulation of intestinal phosphorus and calcium absorption in broiler chickens via 1,25-dihydroxyvitamin D 3
Article Snippet: .. The PVDF membrane was then blocked with 5% nonfat powdered milk in Tris-buffered saline supplemented with tween-20 ( TBST ) at 37°C for 1 h. Subsequently, the membranes were incubated overnight at 4°C with primary antibodies against GAPDH (1:10,000, 60004-1-Ig, Proteintech, Wuhan, China), NaPi-IIb (1:1,000, A9460, ABclonal, Wuhan, China), CaBP-D28k (1:5,000, 14479-1-AP, Proteintech, Wuhan, China), p-ERK (1:1,000, 9101S, Cell Signaling Technology, Boston, USA), t-ERK (1:8,000, 11257-1-AP-50, Proteintech, Wuhan, China), p-p38MAPK (1:1,000, 9216S, Cell Signaling Technology, Boston, USA), and t-p38MAPK (1:1,000, 14064-1-AP, Proteintech, Wuhan, China). ..



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Image Search Results


( A ) Genotype and gender from pups from 9 litters born from two independent heterozygous Slc34a2 breedings. ( B ) body weight, ( C ) food intake, ( D ) water intake, ( E ) fecal output and ( F ) urinary output of wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats fed standard chow (NPD) or challenged for 3 days with low Pi diet (LPD). Data are presented as means ± SEM.

Journal: Scientific Reports

Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

doi: 10.1038/s41598-021-86874-z

Figure Lengend Snippet: ( A ) Genotype and gender from pups from 9 litters born from two independent heterozygous Slc34a2 breedings. ( B ) body weight, ( C ) food intake, ( D ) water intake, ( E ) fecal output and ( F ) urinary output of wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats fed standard chow (NPD) or challenged for 3 days with low Pi diet (LPD). Data are presented as means ± SEM.

Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

Techniques:

( A ) Fecal Pi, ( B ) fecal Ca 2+ , ( C ) urinary Pi, ( D ) urinary Ca 2+ and ( E ) urinary creatinine of wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats fed standard chow (NPD) or challenged for 3 days with low Pi diet (LPD). Data are presented as means ± SEM. Statistical significance was calculated by ANOVA-Bonferroni. * P ≤ 0.05 and **** P ≤ 0.0001.

Journal: Scientific Reports

Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

doi: 10.1038/s41598-021-86874-z

Figure Lengend Snippet: ( A ) Fecal Pi, ( B ) fecal Ca 2+ , ( C ) urinary Pi, ( D ) urinary Ca 2+ and ( E ) urinary creatinine of wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats fed standard chow (NPD) or challenged for 3 days with low Pi diet (LPD). Data are presented as means ± SEM. Statistical significance was calculated by ANOVA-Bonferroni. * P ≤ 0.05 and **** P ≤ 0.0001.

Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

Techniques:

( A ) Plasma Pi, ( B ) plasma Ca 2+ , ( C ) plasma intact FGF-23 and ( D ) plasma 1,25(OH) 2 vitamin D 3 of wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats fed standard chow (NPD) or challenged for 3 days with low Pi diet (LPD). Data are presented as means ± SEM. Statistical significance was calculated by ANOVA-Bonferroni. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.

Journal: Scientific Reports

Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

doi: 10.1038/s41598-021-86874-z

Figure Lengend Snippet: ( A ) Plasma Pi, ( B ) plasma Ca 2+ , ( C ) plasma intact FGF-23 and ( D ) plasma 1,25(OH) 2 vitamin D 3 of wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats fed standard chow (NPD) or challenged for 3 days with low Pi diet (LPD). Data are presented as means ± SEM. Statistical significance was calculated by ANOVA-Bonferroni. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.

Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

Techniques: Clinical Proteomics

Expression of NaPi-IIb in total membranes isolated from mucosa of ( A ) duodenum and ( B ) jejunum from wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats challenged for 3 days with low Pi diet. The abundance of the cotransporter was normalize to the expression of actin. The ratio in WT rats was considered as 100%.

Journal: Scientific Reports

Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

doi: 10.1038/s41598-021-86874-z

Figure Lengend Snippet: Expression of NaPi-IIb in total membranes isolated from mucosa of ( A ) duodenum and ( B ) jejunum from wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats challenged for 3 days with low Pi diet. The abundance of the cotransporter was normalize to the expression of actin. The ratio in WT rats was considered as 100%.

Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

Techniques: Expressing, Isolation

Intestines from ( A , C ) wild type and ( B , D ) Slc34a2 homozygous E18 embryos stained with ( A , B ) H&E or with ( C , D ) a NaPi-IIb antibody. Scale bars: 100 µm in ( A , B ); 50 µm in ( C , D ).

Journal: Scientific Reports

Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

doi: 10.1038/s41598-021-86874-z

Figure Lengend Snippet: Intestines from ( A , C ) wild type and ( B , D ) Slc34a2 homozygous E18 embryos stained with ( A , B ) H&E or with ( C , D ) a NaPi-IIb antibody. Scale bars: 100 µm in ( A , B ); 50 µm in ( C , D ).

Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

Techniques: Staining

Lungs from ( A , C ) wild type and ( B , D ) Slc34a2 homozygous E18 embryos stained with ( A , B ) H&E or with ( C , D ) a NaPi-IIb antibody. Scale bars: 100 µm in ( A , B ); 50 µm in ( C , D ).

Journal: Scientific Reports

Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

doi: 10.1038/s41598-021-86874-z

Figure Lengend Snippet: Lungs from ( A , C ) wild type and ( B , D ) Slc34a2 homozygous E18 embryos stained with ( A , B ) H&E or with ( C , D ) a NaPi-IIb antibody. Scale bars: 100 µm in ( A , B ); 50 µm in ( C , D ).

Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

Techniques: Staining

Liver ( A , B ) and pancreas ( C , D ) from wild type ( A , C ) and Slc34a2 homozygous E18 embryos ( B , D ) stained with a NaPi-IIb antibody. Scale bars: 50 µm.

Journal: Scientific Reports

Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

doi: 10.1038/s41598-021-86874-z

Figure Lengend Snippet: Liver ( A , B ) and pancreas ( C , D ) from wild type ( A , C ) and Slc34a2 homozygous E18 embryos ( B , D ) stained with a NaPi-IIb antibody. Scale bars: 50 µm.

Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

Techniques: Staining

Kidneys from ( A ) wild type and ( B ) Slc34a2 homozygous E18 embryos stained with H&E. Scale bars: 250 µm.

Journal: Scientific Reports

Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

doi: 10.1038/s41598-021-86874-z

Figure Lengend Snippet: Kidneys from ( A ) wild type and ( B ) Slc34a2 homozygous E18 embryos stained with H&E. Scale bars: 250 µm.

Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

Techniques: Staining