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Image Search Results


( A ) Genotype and gender from pups from 9 litters born from two independent heterozygous Slc34a2 breedings. ( B ) body weight, ( C ) food intake, ( D ) water intake, ( E ) fecal output and ( F ) urinary output of wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats fed standard chow (NPD) or challenged for 3 days with low Pi diet (LPD). Data are presented as means ± SEM.

Journal: Scientific Reports

Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

doi: 10.1038/s41598-021-86874-z

Figure Lengend Snippet: ( A ) Genotype and gender from pups from 9 litters born from two independent heterozygous Slc34a2 breedings. ( B ) body weight, ( C ) food intake, ( D ) water intake, ( E ) fecal output and ( F ) urinary output of wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats fed standard chow (NPD) or challenged for 3 days with low Pi diet (LPD). Data are presented as means ± SEM.

Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

Techniques:

( A ) Fecal Pi, ( B ) fecal Ca 2+ , ( C ) urinary Pi, ( D ) urinary Ca 2+ and ( E ) urinary creatinine of wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats fed standard chow (NPD) or challenged for 3 days with low Pi diet (LPD). Data are presented as means ± SEM. Statistical significance was calculated by ANOVA-Bonferroni. * P ≤ 0.05 and **** P ≤ 0.0001.

Journal: Scientific Reports

Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

doi: 10.1038/s41598-021-86874-z

Figure Lengend Snippet: ( A ) Fecal Pi, ( B ) fecal Ca 2+ , ( C ) urinary Pi, ( D ) urinary Ca 2+ and ( E ) urinary creatinine of wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats fed standard chow (NPD) or challenged for 3 days with low Pi diet (LPD). Data are presented as means ± SEM. Statistical significance was calculated by ANOVA-Bonferroni. * P ≤ 0.05 and **** P ≤ 0.0001.

Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

Techniques:

( A ) Plasma Pi, ( B ) plasma Ca 2+ , ( C ) plasma intact FGF-23 and ( D ) plasma 1,25(OH) 2 vitamin D 3 of wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats fed standard chow (NPD) or challenged for 3 days with low Pi diet (LPD). Data are presented as means ± SEM. Statistical significance was calculated by ANOVA-Bonferroni. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.

Journal: Scientific Reports

Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

doi: 10.1038/s41598-021-86874-z

Figure Lengend Snippet: ( A ) Plasma Pi, ( B ) plasma Ca 2+ , ( C ) plasma intact FGF-23 and ( D ) plasma 1,25(OH) 2 vitamin D 3 of wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats fed standard chow (NPD) or challenged for 3 days with low Pi diet (LPD). Data are presented as means ± SEM. Statistical significance was calculated by ANOVA-Bonferroni. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.

Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

Techniques: Clinical Proteomics

Expression of NaPi-IIb in total membranes isolated from mucosa of ( A ) duodenum and ( B ) jejunum from wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats challenged for 3 days with low Pi diet. The abundance of the cotransporter was normalize to the expression of actin. The ratio in WT rats was considered as 100%.

Journal: Scientific Reports

Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

doi: 10.1038/s41598-021-86874-z

Figure Lengend Snippet: Expression of NaPi-IIb in total membranes isolated from mucosa of ( A ) duodenum and ( B ) jejunum from wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats challenged for 3 days with low Pi diet. The abundance of the cotransporter was normalize to the expression of actin. The ratio in WT rats was considered as 100%.

Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

Techniques: Expressing, Isolation

Intestines from ( A , C ) wild type and ( B , D ) Slc34a2 homozygous E18 embryos stained with ( A , B ) H&E or with ( C , D ) a NaPi-IIb antibody. Scale bars: 100 µm in ( A , B ); 50 µm in ( C , D ).

Journal: Scientific Reports

Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

doi: 10.1038/s41598-021-86874-z

Figure Lengend Snippet: Intestines from ( A , C ) wild type and ( B , D ) Slc34a2 homozygous E18 embryos stained with ( A , B ) H&E or with ( C , D ) a NaPi-IIb antibody. Scale bars: 100 µm in ( A , B ); 50 µm in ( C , D ).

Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

Techniques: Staining

Lungs from ( A , C ) wild type and ( B , D ) Slc34a2 homozygous E18 embryos stained with ( A , B ) H&E or with ( C , D ) a NaPi-IIb antibody. Scale bars: 100 µm in ( A , B ); 50 µm in ( C , D ).

Journal: Scientific Reports

Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

doi: 10.1038/s41598-021-86874-z

Figure Lengend Snippet: Lungs from ( A , C ) wild type and ( B , D ) Slc34a2 homozygous E18 embryos stained with ( A , B ) H&E or with ( C , D ) a NaPi-IIb antibody. Scale bars: 100 µm in ( A , B ); 50 µm in ( C , D ).

Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

Techniques: Staining

Liver ( A , B ) and pancreas ( C , D ) from wild type ( A , C ) and Slc34a2 homozygous E18 embryos ( B , D ) stained with a NaPi-IIb antibody. Scale bars: 50 µm.

Journal: Scientific Reports

Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

doi: 10.1038/s41598-021-86874-z

Figure Lengend Snippet: Liver ( A , B ) and pancreas ( C , D ) from wild type ( A , C ) and Slc34a2 homozygous E18 embryos ( B , D ) stained with a NaPi-IIb antibody. Scale bars: 50 µm.

Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

Techniques: Staining

Kidneys from ( A ) wild type and ( B ) Slc34a2 homozygous E18 embryos stained with H&E. Scale bars: 250 µm.

Journal: Scientific Reports

Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

doi: 10.1038/s41598-021-86874-z

Figure Lengend Snippet: Kidneys from ( A ) wild type and ( B ) Slc34a2 homozygous E18 embryos stained with H&E. Scale bars: 250 µm.

Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

Techniques: Staining