small molecule itd1  (Tocris)

Journal: Synthetic and Systems Biotechnology

Article Title: Leveraging ANXA1 to enhance recombinant protein yields in CHO cells: A UPR-Mediated bioprocessing approach

doi: 10.1016/j.synbio.2025.12.001

Figure Lengend Snippet: Schematic overview of the experimental workflow for generating ANXA1-knockdown CHO cell lines and AC2-26 inhibitor treatment. (A) Generation and validation of ANXA1-knockdown cell lines for rADM antibody production. (B) AC2-26 inhibitor treatment in low-producer CHO cells (ADM-14) and subsequent rADM expression analysis.

Article Snippet: On day 3 of suspension culture, the small molecule inhibitor AC2-26 (Topscience Co., Ltd., China) was added [ ], using DMSO (Solarbio Life Sciences, China) as the solvent control.

Techniques: Knockdown, Biomarker Discovery, Expressing

Journal: Synthetic and Systems Biotechnology

Article Title: Leveraging ANXA1 to enhance recombinant protein yields in CHO cells: A UPR-Mediated bioprocessing approach

doi: 10.1016/j.synbio.2025.12.001

Figure Lengend Snippet: AC2-26 effect on ADM-14 CHO cells. (A) Cell density/viability under AC2-26 treatment (n = 3). (B) rADM expression by Western blot with quantification (n = 3). (C) ANXA1 mRNA/protein comparison (n = 3). Quantification was performed using ImageJ (for Western blot densitometry) and GraphPad Prism 10 (for statistical analysis). (∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001). n: represents independent biological replicates.

Article Snippet: On day 3 of suspension culture, the small molecule inhibitor AC2-26 (Topscience Co., Ltd., China) was added [ ], using DMSO (Solarbio Life Sciences, China) as the solvent control.

Techniques: Expressing, Western Blot, Comparison

Journal: Behavioural Pharmacology

Article Title: Naltrexone treatment improves anxiety- and depression-like behavior in alcohol-exposed mice

doi: 10.1097/FBP.0000000000000876

Figure Lengend Snippet: Naltrexone treatment ameliorates microglial activation in alcohol-exposed mice. (a) Immunofluorescence images of microglia labeled with IBA1 (green) in the BLA region (DAPI, blue, for nuclear counterstaining); each image is a representative result from n = 4 independent mice, with images in each treatment group derived from brain tissues of different mice and corresponding coronal sections of the same brain region to ensure intergroup comparability ( n = 4 each group). (b) Quantification of microglial cell numbers in the BLA region ( F = 10.53, DF = 11, P < 0.01; n = 4 each group) * P < 0.05, ** P < 0.01, *** P < 0.001. BLA, basolateral amygdala; DAPI, 4',6-diamidino-2-phenylindole.

Article Snippet: Sections were blocked (5% BSA + 0.5% Triton X-100 in PBS, 1 h, room temperature) and incubated with anti-ionized calcium-binding adapter molecule 1 (IBA1) primary antibody (1 : 500, Proteintech, China, 4°C overnight), followed by Alexa Fluor 488-conjugated secondary antibody (Thermo Fisher Scientific, Waltham, USA, 1 h, room temperature).

Techniques: Activation Assay, Immunofluorescence, Labeling, Derivative Assay