Journal: Journal of Cancer

Article Title: Oridonin inhibits tumor angiogenesis and induces vessel normalization in experimental colon cancer

doi: 10.7150/jca.55929

Figure Lengend Snippet: ORI inhibited angiogenesis of colon cancer. (A) Representative images of immunohistochemical analysis of tumor microvessels (×200, scale bar =100 µm). (B) CD31 + MVD was dramatically suppressed in ORI- treated group compared to the Control group. (C) Representative Western blot expressions of VEGF, bFGF, angiostatin and endostatin. (D and E) The levels of VEGF and bFGF were dramatically down-regulated in the ORI-treated group, while (F and G) angiostatin and endostatin were increased by treatment with ORI. * p < 0.05, ** p < 0.01.

Article Snippet: Sections were then incubated overnight at 4 °C with rabbit anti-Ki67 (1:200, Abcam), rabbit anti-CD31 (1:200, Bioworld), or rabbit anti-HIF-1a (1:100, Boster), then incubated with biotinylated anti-rabbit IgG (1:100, Santa Cruz) for 1 hour at 37 °C, with the avidin-biotinperoxidase complex (1:100, Vector Laboratories) for 1 hour at 37 °C.

Techniques: Immunohistochemical staining, Western Blot

Journal: Journal of Cancer

Article Title: Oridonin inhibits tumor angiogenesis and induces vessel normalization in experimental colon cancer

doi: 10.7150/jca.55929

Figure Lengend Snippet: ORI promoted vessel normalization in colon cancer. (A) Tumor vessels were immunostained for CD31 (green) and pericytes for α-SMA (red). (×400, scale bar =50 µm). (B) The significantly increased perivascular cell coverage in the ORI group was observed from day 5 until day 10 compared to the Control group, the highest of perivascular cell coverage for ORI occurred on day 7.

Article Snippet: Sections were then incubated overnight at 4 °C with rabbit anti-Ki67 (1:200, Abcam), rabbit anti-CD31 (1:200, Bioworld), or rabbit anti-HIF-1a (1:100, Boster), then incubated with biotinylated anti-rabbit IgG (1:100, Santa Cruz) for 1 hour at 37 °C, with the avidin-biotinperoxidase complex (1:100, Vector Laboratories) for 1 hour at 37 °C.

Techniques:

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: MYC promotes the progression of prostate cancer by regulating CD47 to induce an immunosuppressive microenvironment

doi: 10.1007/s00018-026-06109-0

Figure Lengend Snippet: MYC promotes M2 macrophage differentiation by regulating CD47 expression. A Immunofluorescence showed the intercellular localization of MYC and CD47. B-C After knocking down MYC, MYC and CD47 protein and mRNA levels in the cells changed. D The JASPAR database predicted potential MYC-binding sites on the CD47 promoters. E MYC binding to the CD47 promoter was evaluated by ChIP-qPCR assay. F Process of cell co-culture. G Expression levels of the marker gene in M1 and M2 macrophages. H The interaction between SIRPα and CD47 was validated by the Co-IP assay. I-J Differential genes and gene enrichment results were shown. K-L Western blot results showed changes in PI3K/AKT protein expression levels. M Expression levels of the marker gene in M1 and M2 macrophages

Article Snippet: Finally, the sections were counterstained with DAPI for 10 min, washed, and mounted with an anti-fade mounting medium. (Myc: Abcam, ab32072; CD47: Proteintech, 20305-1-AP; CD206: Cell Signaling Technology, 24595 T; CD8: Abcam, ab237709).

Techniques: Expressing, Immunofluorescence, Binding Assay, ChIP-qPCR, Co-Culture Assay, Marker, Co-Immunoprecipitation Assay, Western Blot

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: MYC promotes the progression of prostate cancer by regulating CD47 to induce an immunosuppressive microenvironment

doi: 10.1007/s00018-026-06109-0

Figure Lengend Snippet: The role of macrophages in modulating the functional behaviors of PCa cells. A Cells were co-cultured for display. B-C The proportion of CD8 + T cells was measured by flow cytometry. D Transwell assay results showed the effect of MYC and CD47 expression on the invasion ability of PCa cells. E. EdU assay results showed the effect of MYC and CD47 expression levels on the proliferation of PCa cells. F-G The results of the colony-formation and wound-healing experiments showed the effects of MYC and CD47 expression levels on the proliferation and migration of PCa cells. H Representative multiplex immunofluorescence staining images depict MYC, CD47, CD8 + T and M2 cell in PCa tissues

Article Snippet: Finally, the sections were counterstained with DAPI for 10 min, washed, and mounted with an anti-fade mounting medium. (Myc: Abcam, ab32072; CD47: Proteintech, 20305-1-AP; CD206: Cell Signaling Technology, 24595 T; CD8: Abcam, ab237709).

Techniques: Functional Assay, Cell Culture, Flow Cytometry, Transwell Assay, Expressing, EdU Assay, Migration, Multiplex Assay, Immunofluorescence, Staining