mewo human melanoma cell line  (ATCC)

Journal: Cell reports

Article Title: MITF, TFEB, and TFE3 drive distinct adaptive gene expression programs and immune infiltration in melanoma

doi: 10.1016/j.celrep.2025.116499

Figure Lengend Snippet: (A) Left, western blot of IGR37 melanoma cell line transfected with control or MITF-specific small interfering RNA (siRNA). Right, RT-qPCR for PTEN mRNA from corresponding IGR37 cells. (B and C) Heatmaps showing relative mRNA expression in CCLE melanoma or Tsoi et al. melanoma cell lines. (B) Right, Pearson correlation of gene expression. (D) TCGA melanomas ranked by MITF expression ( MITF ; black line). Gray bars indicate expression of RRAGD or FNIP2 in each melanoma, with the moving average of each per 20 melanoma window indicated by colored lines. (E) MITF ChIP-seq showing binding to the RRAGD or FNIP2 genes. (F) Western blot of melanoma cell lines. (G and H) Box and whisker plots showing relative expression of MITF , RRAGD , and FNIP2 based on triplicate RNA-seq of IGR37 cells expressing doxycycline-inducible β-catenin (iCTNNB1) (G) or in MeWo cells after short hairpin RNA (shRNA)-mediated depletion of MITF (H). See also .

Article Snippet: MeWo Human melanoma cell line (male) , Obtained from the ATCC , Cornil et al. .

Techniques: Western Blot, Transfection, Control, Small Interfering RNA, Quantitative RT-PCR, Expressing, Gene Expression, ChIP-sequencing, Binding Assay, Whisker Assay, RNA Sequencing, shRNA

Journal: bioRxiv

Article Title: Synergistic role of riboflavin-auxotrophic Enterococcus for MR1 expression and intra-tumoral mucosal associated invariant T (MAIT) cell activation

doi: 10.1101/2025.11.25.690539

Figure Lengend Snippet: A, J33-4 MAIT TCR recognition of U266 cells incubated overnight with (inverse black triangles) or without (black circles) 5-OP-RU, in presence or not of E.hirae , E. faecalis , E. faecium , E. coli and B. longum (Left graph) and E. avium (right graph, independent experiment). TCR activation is measured by bioluminescence reading. B , different APC (human dendritic cells, THP1, melanoma MeWO and hepatocellular carcinoma SNU387) were tested for the synergistic effect of E faecalis onto activation of conventional MAIT TCR J33-4 in presence (inverse black triangles) or not (black circles) of 5-OP-RU. C , Flow cytometry analysis of MR1 surface membrane expression using the 26.5 anti MR1 antibody staining of U266, THP1 and dendritic cells (DC) incubated overnight without or with 5-OP-RU in presence or not of E.faecalis . D , Inhibition by Ac-6-FP (escalating doses from 0.2 to 200 uM) of recognition by MAIT TCR J33-4 of MR1 expressed on U266 cultured or not with 5-OP-RU (6.25 uM) in presence (triangles) or not (squares) of E.faecalis (MOI:125). E , MAIT TCR J33-4 activation by Myd88 -/- THP1 cells incubated overnight with (inverse black triangles) or without (black circles) 5-OP-RU (6.25 uM), in presence or not of E. faecalis (MOI:125). F , MAIT TCR J33-4 activation by U266 cells incubated overnight with (inverse black triangles) or without (black circles) 5-OP-RU (6.25 uM), in presence or not of E. faecalis (MOI:125) and E.faecalis derived outer membrane vesicles (OMV). G , Flow cytometry analysis of MR1 surface membrane expression using the 26.5 anti MR1 antibody staining of U266 incubated overnight without or with 5-OP-RU in presence or not of E. faecalis (MOI:125), compared to LPS (TLR4 ligand; 50ng/ml; left histogram), CPG (TLR9 ligand; 5uM, middle histogram) or LTA (TLR2 ligand; 30ug/ml co; right histogram). All data are shown as mean ± SEM with technical replicates represented by number of symbols in each graph. ****:P<0.0001, ***:0.0001

Article Snippet: MeWo and SNU387 cell lines were kindly provided by Dr. Scott Wilson’s lab and cul-tured according to ATCC protocol.

Techniques: Incubation, Activation Assay, Flow Cytometry, Membrane, Expressing, Staining, Inhibition, Cell Culture, Derivative Assay, Two Tailed Test