Journal: Plants

Article Title: Screening for Selective Anticancer Activity of 65 Extracts of Plants Collected in Western Andalusia, Spain

doi: 10.3390/plants10102193

Figure Lengend Snippet: Cytotoxic activity of Tetraclinis articulata (Vahl) Mast. ( 58 ) against 14 cancer cell lines from a variety of tissues.

Article Snippet: HNO97 (human tongue cancer cells), A64-CLS (human submaxillary gland adenoma cells), AN3Ca (human endometrial adenocarcinoma cells), Sk-OV-3 (human ovarian cancer cells), KATO III (human gastric cancer cells), Sk-Br-3 (HER2-positive breast cancer cells), T24 (human bladder cancer cells), Calu-1 (human squamous lung cancer cells), and MeWo (human BRAF wild-type melanoma cells) were purchased from Cell Lines Service (CLS).

Techniques: Activity Assay

Journal: The Journal of Clinical Investigation

Article Title: Comparative oncogenomics identifies tyrosine kinase FES as a tumor suppressor in melanoma

doi: 10.1172/JCI91291

Figure Lengend Snippet: (A) Western blot analysis of FES in MeWo cell line with overexpression of FES. Actin served as a loading control. (B) Clonogenic assay of MeWo cells with and without expression of exogenous FES. Error bars indicate mean ± SEM (n = 4 biological replicates per group). (C) Soft agar assay of MeWo cells with and without expression of exogenous FES. Error bars indicate mean ± SEM (n = 3 biological replicates per group). (D) Mice (n = 9) were injected with 1 × 106 MeWo cells to the left or MeWo cells expressing exogenous FES to the right side of the body. The tumor volume was measured every 3 to 4 days up to day 24. Error bars indicate mean ± SEM (n = 9 biological replicates per group). (E) Schematic indicating the location of the FES kinase-dead mutation. F-B, F-BAR domain. (F) Western blot analysis of doxycycline-induced expression of WT or mutant FES in MeWo cell line. Vinculin served as a loading control. (G) Clonogenic assay of MeWo cells expressing WT or mutant FES. Quantification of the assay measured as the percentage of area covered is shown in the graph on the right. Error bars indicate mean ± SEM (n = 3 biological replicates per group). Statistical significance was determined by unpaired 2-sided t test (B, G) and 2-way ANOVA (C, D).

Article Snippet: MeWo cells (1 million) were injected in 150 μl of 0.9% NaCl subcutaneously into nude mice (Janvier).

Techniques: Western Blot, Over Expression, Control, Clonogenic Assay, Expressing, Soft Agar Assay, Injection, Mutagenesis

Journal: International Journal of Molecular Sciences

Article Title: Human Microfibrillar-Associated Protein 4 (MFAP4) Gene Promoter: A TATA-Less Promoter That Is Regulated by Retinol and Coenzyme Q10 in Human Fibroblast Cells

doi: 10.3390/ijms21218392

Figure Lengend Snippet: The expression of pEGFP- pMFAP4 plasmids in mouse fibroblast NIH/3T3 cells, human melanoma MeWo cells, and mouse melanoma B16-F10 cells. ( A ) Fluorescence images. Scale bar = 100 μm. ( B ) Relative fluorescence intensity. Data are presented as means ± SE.

Article Snippet: Detroit 551 cells (BCRC 60118, human normal fibroblast cells), MeWo cells (BCRC 60540, human melanoma cells), NIH/3T3 cells (BCRC 60008, mouse fibroblast cells), and B16-F10 cells (BCRC 60031, mouse melanoma cells) were all obtained from the Bioresource Collection and Research Center (BCRC, Hsinchu, Taiwan, ROC).

Techniques: Expressing, Fluorescence

Journal: British Journal of Cancer

Article Title: Clinically proven markers of metastasis predict metastatic spread of human melanoma cells engrafted in scid mice

doi: 10.1038/sj.bjc.6603594

Figure Lengend Snippet: (a) Characteristics of six human melanoma cell lines xenografted into scid mice ( n =10 per cell line) and (b) glycoconjugate expression of their respective primary tumours and spontaneous lung metastasis

Article Snippet: The human melanoma cell lines UISO-Mel6 (established from a primary malignant melanoma; see Rauth et al (1998) ), MV3 (established from a metastatic melanoma lymph node; see ) and MeWo (established from a metastatic melanoma lymph node of a white, 78-year-old male; see Carey et al (1976) ) were kindly provided by the Klinik für Dermatologie, Universitätsklinikum Hamburg-Eppendorf, Germany.

Techniques: Mouse Assay, Expressing

Journal: British Journal of Cancer

Article Title: Clinically proven markers of metastasis predict metastatic spread of human melanoma cells engrafted in scid mice

doi: 10.1038/sj.bjc.6603594

Figure Lengend Snippet: Correlation of tumour weight and number of lung metastasis of human melanoma cell lines subcutaneously xenografted into scid mice

Article Snippet: The human melanoma cell lines UISO-Mel6 (established from a primary malignant melanoma; see Rauth et al (1998) ), MV3 (established from a metastatic melanoma lymph node; see ) and MeWo (established from a metastatic melanoma lymph node of a white, 78-year-old male; see Carey et al (1976) ) were kindly provided by the Klinik für Dermatologie, Universitätsklinikum Hamburg-Eppendorf, Germany.

Techniques:

Journal: British Journal of Cancer

Article Title: Clinically proven markers of metastasis predict metastatic spread of human melanoma cells engrafted in scid mice

doi: 10.1038/sj.bjc.6603594

Figure Lengend Snippet: Comparative analysis of glycoconjugate expression of six human melanoma cell lines in vitro (a) and in vivo (b)

Article Snippet: The human melanoma cell lines UISO-Mel6 (established from a primary malignant melanoma; see Rauth et al (1998) ), MV3 (established from a metastatic melanoma lymph node; see ) and MeWo (established from a metastatic melanoma lymph node of a white, 78-year-old male; see Carey et al (1976) ) were kindly provided by the Klinik für Dermatologie, Universitätsklinikum Hamburg-Eppendorf, Germany.

Techniques: Expressing, In Vitro, In Vivo