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mcc950  (MedChemExpress)


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    Structured Review

    MedChemExpress mcc950
    Mcc950, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 641 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcc950/MCC950/pm42840853-278-2-6
    Average 99 stars, based on 641 article reviews
    mcc950 - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Adsorption:

    Article Title: A nucleic acid regulation strategy under mechanical stress for intervertebral disc degeneration treatment
    Article Snippet: ption capacity assay of SiNPs with different N/P ratios was evaluated by agarose gel electrophoresis. Besides, HA-F127 SiNPs were characterized by electron microscope and Zeta-Nanosizer. Next, MCC950 (MCE, HY-12815), dissolved in PBS, was combined with HA-F127 SiNPs to achieve a final concentration of 1 mg/mL. Drug release was assessed by monitoring the analyte concentration at 230 nm using HPLC.Th

    Article Title: Engineering biomimetic coronary artery equivalents for Kawasaki disease modeling and drug screening.
    Article Snippet: ATOR and MCC950 were purchased from MedChemExpress (MCE, USA) and dissolved in dimethyl sulfoxide (DMSO) to prepare 10 mM stock solutions.. To determine non-cytotoxic working concentrations and rule out high-dose cytotoxicity, dose-response cell viability assays were first performed.To determine non-cytotoxic working concentrations and rule out high-dose cytotoxicity, dose-response cell viability assays were first performed.

    Article Title: Angelica sinensis polysaccharide inhibits pyroptosis and improves osteoarthritis through the collaborative power of PPARγ and Nrf2
    Article Snippet: It was dissolved in DMSO.It was dissolved in DMSO.. GW1929, NK-252, N-acetylcysteine (NAC), and MCC950 were purchased from MedChemExpress (Shanghai, China) and dissolved in DMSO.. HE and safranin O/fast green staining kits were purchased from Solarbio (Beijing, China).HE and safranin O/fast green staining kits were purchased from Solarbio (Beijing, China).

    Article Title: Microglia-specific NLRP3 inhibition mitigates hippocampal neuroinflammation and cognitive deficits after hemorrhagic shock with resuscitation.
    Article Snippet: Mice were euthanized by cervical dislocation if their blood pressure did not return to normal after the transfusion.Mice were euthanized by cervical dislocation if their blood pressure did not return to normal after the transfusion.. 2 μl of MCC950 (10 mg/kg, # HY-12815A/CS-5427, MedChemExpress, Shanghai, China) was administered into the left lateral ventricle (0.5 mm behind the anterior fontanelle, 1 mm left to the median, 2.5 mm below the skull surface) immediately after model establishment (Fig. 1).. ICV injection was chosen to achieve high and consistent brain bioavailability of MCC950, bypassing the blood-brain barrier, which the compound penetrates poorly, and to restrict NLRP3 inhibition primarily to the CNS compartment for mechanistic specificity.ICV injection was chosen to achieve high and consistent brain bioavailability of MCC950, bypassing the blood-brain barrier, which the compound penetrates poorly, and to restrict NLRP3 inhibition primarily to the CNS compartment for mechanistic specificity.

    High Performance Liquid Chromatography:

    Article Title: A nucleic acid regulation strategy under mechanical stress for intervertebral disc degeneration treatment
    Article Snippet: ption capacity assay of SiNPs with different N/P ratios was evaluated by agarose gel electrophoresis. Besides, HA-F127 SiNPs were characterized by electron microscope and Zeta-Nanosizer. Next, MCC950 (MCE, HY-12815), dissolved in PBS, was combined with HA-F127 SiNPs to achieve a final concentration of 1 mg/mL. Drug release was assessed by monitoring the analyte concentration at 230 nm using HPLC.Th

    Article Title: Engineering biomimetic coronary artery equivalents for Kawasaki disease modeling and drug screening.
    Article Snippet: ATOR and MCC950 were purchased from MedChemExpress (MCE, USA) and dissolved in dimethyl sulfoxide (DMSO) to prepare 10 mM stock solutions.. To determine non-cytotoxic working concentrations and rule out high-dose cytotoxicity, dose-response cell viability assays were first performed.To determine non-cytotoxic working concentrations and rule out high-dose cytotoxicity, dose-response cell viability assays were first performed.

    Article Title: Angelica sinensis polysaccharide inhibits pyroptosis and improves osteoarthritis through the collaborative power of PPARγ and Nrf2
    Article Snippet: It was dissolved in DMSO.It was dissolved in DMSO.. GW1929, NK-252, N-acetylcysteine (NAC), and MCC950 were purchased from MedChemExpress (Shanghai, China) and dissolved in DMSO.. HE and safranin O/fast green staining kits were purchased from Solarbio (Beijing, China).HE and safranin O/fast green staining kits were purchased from Solarbio (Beijing, China).

    Article Title: Microglia-specific NLRP3 inhibition mitigates hippocampal neuroinflammation and cognitive deficits after hemorrhagic shock with resuscitation.
    Article Snippet: Mice were euthanized by cervical dislocation if their blood pressure did not return to normal after the transfusion.Mice were euthanized by cervical dislocation if their blood pressure did not return to normal after the transfusion.. 2 μl of MCC950 (10 mg/kg, # HY-12815A/CS-5427, MedChemExpress, Shanghai, China) was administered into the left lateral ventricle (0.5 mm behind the anterior fontanelle, 1 mm left to the median, 2.5 mm below the skull surface) immediately after model establishment (Fig. 1).. ICV injection was chosen to achieve high and consistent brain bioavailability of MCC950, bypassing the blood-brain barrier, which the compound penetrates poorly, and to restrict NLRP3 inhibition primarily to the CNS compartment for mechanistic specificity.ICV injection was chosen to achieve high and consistent brain bioavailability of MCC950, bypassing the blood-brain barrier, which the compound penetrates poorly, and to restrict NLRP3 inhibition primarily to the CNS compartment for mechanistic specificity.

    Concentration Assay:

    Article Title: A nucleic acid regulation strategy under mechanical stress for intervertebral disc degeneration treatment
    Article Snippet: ption capacity assay of SiNPs with different N/P ratios was evaluated by agarose gel electrophoresis. Besides, HA-F127 SiNPs were characterized by electron microscope and Zeta-Nanosizer. Next, MCC950 (MCE, HY-12815), dissolved in PBS, was combined with HA-F127 SiNPs to achieve a final concentration of 1 mg/mL. Drug release was assessed by monitoring the analyte concentration at 230 nm using HPLC.Th

    Article Title: Engineering biomimetic coronary artery equivalents for Kawasaki disease modeling and drug screening.
    Article Snippet: ATOR and MCC950 were purchased from MedChemExpress (MCE, USA) and dissolved in dimethyl sulfoxide (DMSO) to prepare 10 mM stock solutions.. To determine non-cytotoxic working concentrations and rule out high-dose cytotoxicity, dose-response cell viability assays were first performed.To determine non-cytotoxic working concentrations and rule out high-dose cytotoxicity, dose-response cell viability assays were first performed.

    Article Title: Angelica sinensis polysaccharide inhibits pyroptosis and improves osteoarthritis through the collaborative power of PPARγ and Nrf2
    Article Snippet: It was dissolved in DMSO.It was dissolved in DMSO.. GW1929, NK-252, N-acetylcysteine (NAC), and MCC950 were purchased from MedChemExpress (Shanghai, China) and dissolved in DMSO.. HE and safranin O/fast green staining kits were purchased from Solarbio (Beijing, China).HE and safranin O/fast green staining kits were purchased from Solarbio (Beijing, China).

    Article Title: Microglia-specific NLRP3 inhibition mitigates hippocampal neuroinflammation and cognitive deficits after hemorrhagic shock with resuscitation.
    Article Snippet: Mice were euthanized by cervical dislocation if their blood pressure did not return to normal after the transfusion.Mice were euthanized by cervical dislocation if their blood pressure did not return to normal after the transfusion.. 2 μl of MCC950 (10 mg/kg, # HY-12815A/CS-5427, MedChemExpress, Shanghai, China) was administered into the left lateral ventricle (0.5 mm behind the anterior fontanelle, 1 mm left to the median, 2.5 mm below the skull surface) immediately after model establishment (Fig. 1).. ICV injection was chosen to achieve high and consistent brain bioavailability of MCC950, bypassing the blood-brain barrier, which the compound penetrates poorly, and to restrict NLRP3 inhibition primarily to the CNS compartment for mechanistic specificity.ICV injection was chosen to achieve high and consistent brain bioavailability of MCC950, bypassing the blood-brain barrier, which the compound penetrates poorly, and to restrict NLRP3 inhibition primarily to the CNS compartment for mechanistic specificity.

    Electrophoresis:

    Article Title: A nucleic acid regulation strategy under mechanical stress for intervertebral disc degeneration treatment
    Article Snippet: ption capacity assay of SiNPs with different N/P ratios was evaluated by agarose gel electrophoresis. Besides, HA-F127 SiNPs were characterized by electron microscope and Zeta-Nanosizer. Next, MCC950 (MCE, HY-12815), dissolved in PBS, was combined with HA-F127 SiNPs to achieve a final concentration of 1 mg/mL. Drug release was assessed by monitoring the analyte concentration at 230 nm using HPLC.Th

    Article Title: Engineering biomimetic coronary artery equivalents for Kawasaki disease modeling and drug screening.
    Article Snippet: ATOR and MCC950 were purchased from MedChemExpress (MCE, USA) and dissolved in dimethyl sulfoxide (DMSO) to prepare 10 mM stock solutions.. To determine non-cytotoxic working concentrations and rule out high-dose cytotoxicity, dose-response cell viability assays were first performed.To determine non-cytotoxic working concentrations and rule out high-dose cytotoxicity, dose-response cell viability assays were first performed.

    Article Title: Angelica sinensis polysaccharide inhibits pyroptosis and improves osteoarthritis through the collaborative power of PPARγ and Nrf2
    Article Snippet: It was dissolved in DMSO.It was dissolved in DMSO.. GW1929, NK-252, N-acetylcysteine (NAC), and MCC950 were purchased from MedChemExpress (Shanghai, China) and dissolved in DMSO.. HE and safranin O/fast green staining kits were purchased from Solarbio (Beijing, China).HE and safranin O/fast green staining kits were purchased from Solarbio (Beijing, China).

    Article Title: Microglia-specific NLRP3 inhibition mitigates hippocampal neuroinflammation and cognitive deficits after hemorrhagic shock with resuscitation.
    Article Snippet: Mice were euthanized by cervical dislocation if their blood pressure did not return to normal after the transfusion.Mice were euthanized by cervical dislocation if their blood pressure did not return to normal after the transfusion.. 2 μl of MCC950 (10 mg/kg, # HY-12815A/CS-5427, MedChemExpress, Shanghai, China) was administered into the left lateral ventricle (0.5 mm behind the anterior fontanelle, 1 mm left to the median, 2.5 mm below the skull surface) immediately after model establishment (Fig. 1).. ICV injection was chosen to achieve high and consistent brain bioavailability of MCC950, bypassing the blood-brain barrier, which the compound penetrates poorly, and to restrict NLRP3 inhibition primarily to the CNS compartment for mechanistic specificity.ICV injection was chosen to achieve high and consistent brain bioavailability of MCC950, bypassing the blood-brain barrier, which the compound penetrates poorly, and to restrict NLRP3 inhibition primarily to the CNS compartment for mechanistic specificity.

    Agarose Gel Electrophoresis:

    Article Title: A nucleic acid regulation strategy under mechanical stress for intervertebral disc degeneration treatment
    Article Snippet: ption capacity assay of SiNPs with different N/P ratios was evaluated by agarose gel electrophoresis. Besides, HA-F127 SiNPs were characterized by electron microscope and Zeta-Nanosizer. Next, MCC950 (MCE, HY-12815), dissolved in PBS, was combined with HA-F127 SiNPs to achieve a final concentration of 1 mg/mL. Drug release was assessed by monitoring the analyte concentration at 230 nm using HPLC.Th

    Article Title: Engineering biomimetic coronary artery equivalents for Kawasaki disease modeling and drug screening.
    Article Snippet: ATOR and MCC950 were purchased from MedChemExpress (MCE, USA) and dissolved in dimethyl sulfoxide (DMSO) to prepare 10 mM stock solutions.. To determine non-cytotoxic working concentrations and rule out high-dose cytotoxicity, dose-response cell viability assays were first performed.To determine non-cytotoxic working concentrations and rule out high-dose cytotoxicity, dose-response cell viability assays were first performed.

    Article Title: Angelica sinensis polysaccharide inhibits pyroptosis and improves osteoarthritis through the collaborative power of PPARγ and Nrf2
    Article Snippet: It was dissolved in DMSO.It was dissolved in DMSO.. GW1929, NK-252, N-acetylcysteine (NAC), and MCC950 were purchased from MedChemExpress (Shanghai, China) and dissolved in DMSO.. HE and safranin O/fast green staining kits were purchased from Solarbio (Beijing, China).HE and safranin O/fast green staining kits were purchased from Solarbio (Beijing, China).

    Article Title: Microglia-specific NLRP3 inhibition mitigates hippocampal neuroinflammation and cognitive deficits after hemorrhagic shock with resuscitation.
    Article Snippet: Mice were euthanized by cervical dislocation if their blood pressure did not return to normal after the transfusion.Mice were euthanized by cervical dislocation if their blood pressure did not return to normal after the transfusion.. 2 μl of MCC950 (10 mg/kg, # HY-12815A/CS-5427, MedChemExpress, Shanghai, China) was administered into the left lateral ventricle (0.5 mm behind the anterior fontanelle, 1 mm left to the median, 2.5 mm below the skull surface) immediately after model establishment (Fig. 1).. ICV injection was chosen to achieve high and consistent brain bioavailability of MCC950, bypassing the blood-brain barrier, which the compound penetrates poorly, and to restrict NLRP3 inhibition primarily to the CNS compartment for mechanistic specificity.ICV injection was chosen to achieve high and consistent brain bioavailability of MCC950, bypassing the blood-brain barrier, which the compound penetrates poorly, and to restrict NLRP3 inhibition primarily to the CNS compartment for mechanistic specificity.

    Microscopy:

    Article Title: A nucleic acid regulation strategy under mechanical stress for intervertebral disc degeneration treatment
    Article Snippet: ption capacity assay of SiNPs with different N/P ratios was evaluated by agarose gel electrophoresis. Besides, HA-F127 SiNPs were characterized by electron microscope and Zeta-Nanosizer. Next, MCC950 (MCE, HY-12815), dissolved in PBS, was combined with HA-F127 SiNPs to achieve a final concentration of 1 mg/mL. Drug release was assessed by monitoring the analyte concentration at 230 nm using HPLC.Th

    Article Title: Engineering biomimetic coronary artery equivalents for Kawasaki disease modeling and drug screening.
    Article Snippet: ATOR and MCC950 were purchased from MedChemExpress (MCE, USA) and dissolved in dimethyl sulfoxide (DMSO) to prepare 10 mM stock solutions.. To determine non-cytotoxic working concentrations and rule out high-dose cytotoxicity, dose-response cell viability assays were first performed.To determine non-cytotoxic working concentrations and rule out high-dose cytotoxicity, dose-response cell viability assays were first performed.

    Article Title: Angelica sinensis polysaccharide inhibits pyroptosis and improves osteoarthritis through the collaborative power of PPARγ and Nrf2
    Article Snippet: It was dissolved in DMSO.It was dissolved in DMSO.. GW1929, NK-252, N-acetylcysteine (NAC), and MCC950 were purchased from MedChemExpress (Shanghai, China) and dissolved in DMSO.. HE and safranin O/fast green staining kits were purchased from Solarbio (Beijing, China).HE and safranin O/fast green staining kits were purchased from Solarbio (Beijing, China).

    Article Title: Microglia-specific NLRP3 inhibition mitigates hippocampal neuroinflammation and cognitive deficits after hemorrhagic shock with resuscitation.
    Article Snippet: Mice were euthanized by cervical dislocation if their blood pressure did not return to normal after the transfusion.Mice were euthanized by cervical dislocation if their blood pressure did not return to normal after the transfusion.. 2 μl of MCC950 (10 mg/kg, # HY-12815A/CS-5427, MedChemExpress, Shanghai, China) was administered into the left lateral ventricle (0.5 mm behind the anterior fontanelle, 1 mm left to the median, 2.5 mm below the skull surface) immediately after model establishment (Fig. 1).. ICV injection was chosen to achieve high and consistent brain bioavailability of MCC950, bypassing the blood-brain barrier, which the compound penetrates poorly, and to restrict NLRP3 inhibition primarily to the CNS compartment for mechanistic specificity.ICV injection was chosen to achieve high and consistent brain bioavailability of MCC950, bypassing the blood-brain barrier, which the compound penetrates poorly, and to restrict NLRP3 inhibition primarily to the CNS compartment for mechanistic specificity.

    Nucleic Acid Electrophoresis:

    Article Title: A nucleic acid regulation strategy under mechanical stress for intervertebral disc degeneration treatment
    Article Snippet: ption capacity assay of SiNPs with different N/P ratios was evaluated by agarose gel electrophoresis. Besides, HA-F127 SiNPs were characterized by electron microscope and Zeta-Nanosizer. Next, MCC950 (MCE, HY-12815), dissolved in PBS, was combined with HA-F127 SiNPs to achieve a final concentration of 1 mg/mL. Drug release was assessed by monitoring the analyte concentration at 230 nm using HPLC.Th

    Article Title: Engineering biomimetic coronary artery equivalents for Kawasaki disease modeling and drug screening.
    Article Snippet: ATOR and MCC950 were purchased from MedChemExpress (MCE, USA) and dissolved in dimethyl sulfoxide (DMSO) to prepare 10 mM stock solutions.. To determine non-cytotoxic working concentrations and rule out high-dose cytotoxicity, dose-response cell viability assays were first performed.To determine non-cytotoxic working concentrations and rule out high-dose cytotoxicity, dose-response cell viability assays were first performed.

    Article Title: Angelica sinensis polysaccharide inhibits pyroptosis and improves osteoarthritis through the collaborative power of PPARγ and Nrf2
    Article Snippet: It was dissolved in DMSO.It was dissolved in DMSO.. GW1929, NK-252, N-acetylcysteine (NAC), and MCC950 were purchased from MedChemExpress (Shanghai, China) and dissolved in DMSO.. HE and safranin O/fast green staining kits were purchased from Solarbio (Beijing, China).HE and safranin O/fast green staining kits were purchased from Solarbio (Beijing, China).

    Article Title: Microglia-specific NLRP3 inhibition mitigates hippocampal neuroinflammation and cognitive deficits after hemorrhagic shock with resuscitation.
    Article Snippet: Mice were euthanized by cervical dislocation if their blood pressure did not return to normal after the transfusion.Mice were euthanized by cervical dislocation if their blood pressure did not return to normal after the transfusion.. 2 μl of MCC950 (10 mg/kg, # HY-12815A/CS-5427, MedChemExpress, Shanghai, China) was administered into the left lateral ventricle (0.5 mm behind the anterior fontanelle, 1 mm left to the median, 2.5 mm below the skull surface) immediately after model establishment (Fig. 1).. ICV injection was chosen to achieve high and consistent brain bioavailability of MCC950, bypassing the blood-brain barrier, which the compound penetrates poorly, and to restrict NLRP3 inhibition primarily to the CNS compartment for mechanistic specificity.ICV injection was chosen to achieve high and consistent brain bioavailability of MCC950, bypassing the blood-brain barrier, which the compound penetrates poorly, and to restrict NLRP3 inhibition primarily to the CNS compartment for mechanistic specificity.

    Liposomes:

    Article Title: A nucleic acid regulation strategy under mechanical stress for intervertebral disc degeneration treatment
    Article Snippet: ption capacity assay of SiNPs with different N/P ratios was evaluated by agarose gel electrophoresis. Besides, HA-F127 SiNPs were characterized by electron microscope and Zeta-Nanosizer. Next, MCC950 (MCE, HY-12815), dissolved in PBS, was combined with HA-F127 SiNPs to achieve a final concentration of 1 mg/mL. Drug release was assessed by monitoring the analyte concentration at 230 nm using HPLC.Th

    Article Title: Engineering biomimetic coronary artery equivalents for Kawasaki disease modeling and drug screening.
    Article Snippet: ATOR and MCC950 were purchased from MedChemExpress (MCE, USA) and dissolved in dimethyl sulfoxide (DMSO) to prepare 10 mM stock solutions.. To determine non-cytotoxic working concentrations and rule out high-dose cytotoxicity, dose-response cell viability assays were first performed.To determine non-cytotoxic working concentrations and rule out high-dose cytotoxicity, dose-response cell viability assays were first performed.

    Article Title: Angelica sinensis polysaccharide inhibits pyroptosis and improves osteoarthritis through the collaborative power of PPARγ and Nrf2
    Article Snippet: It was dissolved in DMSO.It was dissolved in DMSO.. GW1929, NK-252, N-acetylcysteine (NAC), and MCC950 were purchased from MedChemExpress (Shanghai, China) and dissolved in DMSO.. HE and safranin O/fast green staining kits were purchased from Solarbio (Beijing, China).HE and safranin O/fast green staining kits were purchased from Solarbio (Beijing, China).

    Article Title: Microglia-specific NLRP3 inhibition mitigates hippocampal neuroinflammation and cognitive deficits after hemorrhagic shock with resuscitation.
    Article Snippet: Mice were euthanized by cervical dislocation if their blood pressure did not return to normal after the transfusion.Mice were euthanized by cervical dislocation if their blood pressure did not return to normal after the transfusion.. 2 μl of MCC950 (10 mg/kg, # HY-12815A/CS-5427, MedChemExpress, Shanghai, China) was administered into the left lateral ventricle (0.5 mm behind the anterior fontanelle, 1 mm left to the median, 2.5 mm below the skull surface) immediately after model establishment (Fig. 1).. ICV injection was chosen to achieve high and consistent brain bioavailability of MCC950, bypassing the blood-brain barrier, which the compound penetrates poorly, and to restrict NLRP3 inhibition primarily to the CNS compartment for mechanistic specificity.ICV injection was chosen to achieve high and consistent brain bioavailability of MCC950, bypassing the blood-brain barrier, which the compound penetrates poorly, and to restrict NLRP3 inhibition primarily to the CNS compartment for mechanistic specificity.

    Immunoprecipitation:

    Article Title: A nucleic acid regulation strategy under mechanical stress for intervertebral disc degeneration treatment
    Article Snippet: ption capacity assay of SiNPs with different N/P ratios was evaluated by agarose gel electrophoresis. Besides, HA-F127 SiNPs were characterized by electron microscope and Zeta-Nanosizer. Next, MCC950 (MCE, HY-12815), dissolved in PBS, was combined with HA-F127 SiNPs to achieve a final concentration of 1 mg/mL. Drug release was assessed by monitoring the analyte concentration at 230 nm using HPLC.Th

    Article Title: Engineering biomimetic coronary artery equivalents for Kawasaki disease modeling and drug screening.
    Article Snippet: ATOR and MCC950 were purchased from MedChemExpress (MCE, USA) and dissolved in dimethyl sulfoxide (DMSO) to prepare 10 mM stock solutions.. To determine non-cytotoxic working concentrations and rule out high-dose cytotoxicity, dose-response cell viability assays were first performed.To determine non-cytotoxic working concentrations and rule out high-dose cytotoxicity, dose-response cell viability assays were first performed.

    Article Title: Angelica sinensis polysaccharide inhibits pyroptosis and improves osteoarthritis through the collaborative power of PPARγ and Nrf2
    Article Snippet: It was dissolved in DMSO.It was dissolved in DMSO.. GW1929, NK-252, N-acetylcysteine (NAC), and MCC950 were purchased from MedChemExpress (Shanghai, China) and dissolved in DMSO.. HE and safranin O/fast green staining kits were purchased from Solarbio (Beijing, China).HE and safranin O/fast green staining kits were purchased from Solarbio (Beijing, China).

    Article Title: Microglia-specific NLRP3 inhibition mitigates hippocampal neuroinflammation and cognitive deficits after hemorrhagic shock with resuscitation.
    Article Snippet: Mice were euthanized by cervical dislocation if their blood pressure did not return to normal after the transfusion.Mice were euthanized by cervical dislocation if their blood pressure did not return to normal after the transfusion.. 2 μl of MCC950 (10 mg/kg, # HY-12815A/CS-5427, MedChemExpress, Shanghai, China) was administered into the left lateral ventricle (0.5 mm behind the anterior fontanelle, 1 mm left to the median, 2.5 mm below the skull surface) immediately after model establishment (Fig. 1).. ICV injection was chosen to achieve high and consistent brain bioavailability of MCC950, bypassing the blood-brain barrier, which the compound penetrates poorly, and to restrict NLRP3 inhibition primarily to the CNS compartment for mechanistic specificity.ICV injection was chosen to achieve high and consistent brain bioavailability of MCC950, bypassing the blood-brain barrier, which the compound penetrates poorly, and to restrict NLRP3 inhibition primarily to the CNS compartment for mechanistic specificity.

    Recombinant:

    Article Title: A nucleic acid regulation strategy under mechanical stress for intervertebral disc degeneration treatment
    Article Snippet: ption capacity assay of SiNPs with different N/P ratios was evaluated by agarose gel electrophoresis. Besides, HA-F127 SiNPs were characterized by electron microscope and Zeta-Nanosizer. Next, MCC950 (MCE, HY-12815), dissolved in PBS, was combined with HA-F127 SiNPs to achieve a final concentration of 1 mg/mL. Drug release was assessed by monitoring the analyte concentration at 230 nm using HPLC.Th

    Article Title: Engineering biomimetic coronary artery equivalents for Kawasaki disease modeling and drug screening.
    Article Snippet: ATOR and MCC950 were purchased from MedChemExpress (MCE, USA) and dissolved in dimethyl sulfoxide (DMSO) to prepare 10 mM stock solutions.. To determine non-cytotoxic working concentrations and rule out high-dose cytotoxicity, dose-response cell viability assays were first performed.To determine non-cytotoxic working concentrations and rule out high-dose cytotoxicity, dose-response cell viability assays were first performed.

    Article Title: Angelica sinensis polysaccharide inhibits pyroptosis and improves osteoarthritis through the collaborative power of PPARγ and Nrf2
    Article Snippet: It was dissolved in DMSO.It was dissolved in DMSO.. GW1929, NK-252, N-acetylcysteine (NAC), and MCC950 were purchased from MedChemExpress (Shanghai, China) and dissolved in DMSO.. HE and safranin O/fast green staining kits were purchased from Solarbio (Beijing, China).HE and safranin O/fast green staining kits were purchased from Solarbio (Beijing, China).

    Article Title: Microglia-specific NLRP3 inhibition mitigates hippocampal neuroinflammation and cognitive deficits after hemorrhagic shock with resuscitation.
    Article Snippet: Mice were euthanized by cervical dislocation if their blood pressure did not return to normal after the transfusion.Mice were euthanized by cervical dislocation if their blood pressure did not return to normal after the transfusion.. 2 μl of MCC950 (10 mg/kg, # HY-12815A/CS-5427, MedChemExpress, Shanghai, China) was administered into the left lateral ventricle (0.5 mm behind the anterior fontanelle, 1 mm left to the median, 2.5 mm below the skull surface) immediately after model establishment (Fig. 1).. ICV injection was chosen to achieve high and consistent brain bioavailability of MCC950, bypassing the blood-brain barrier, which the compound penetrates poorly, and to restrict NLRP3 inhibition primarily to the CNS compartment for mechanistic specificity.ICV injection was chosen to achieve high and consistent brain bioavailability of MCC950, bypassing the blood-brain barrier, which the compound penetrates poorly, and to restrict NLRP3 inhibition primarily to the CNS compartment for mechanistic specificity.

    Mouse Assay:

    Article Title: A nucleic acid regulation strategy under mechanical stress for intervertebral disc degeneration treatment
    Article Snippet: ption capacity assay of SiNPs with different N/P ratios was evaluated by agarose gel electrophoresis. Besides, HA-F127 SiNPs were characterized by electron microscope and Zeta-Nanosizer. Next, MCC950 (MCE, HY-12815), dissolved in PBS, was combined with HA-F127 SiNPs to achieve a final concentration of 1 mg/mL. Drug release was assessed by monitoring the analyte concentration at 230 nm using HPLC.Th

    Article Title: Engineering biomimetic coronary artery equivalents for Kawasaki disease modeling and drug screening.
    Article Snippet: ATOR and MCC950 were purchased from MedChemExpress (MCE, USA) and dissolved in dimethyl sulfoxide (DMSO) to prepare 10 mM stock solutions.. To determine non-cytotoxic working concentrations and rule out high-dose cytotoxicity, dose-response cell viability assays were first performed.To determine non-cytotoxic working concentrations and rule out high-dose cytotoxicity, dose-response cell viability assays were first performed.

    Article Title: Angelica sinensis polysaccharide inhibits pyroptosis and improves osteoarthritis through the collaborative power of PPARγ and Nrf2
    Article Snippet: It was dissolved in DMSO.It was dissolved in DMSO.. GW1929, NK-252, N-acetylcysteine (NAC), and MCC950 were purchased from MedChemExpress (Shanghai, China) and dissolved in DMSO.. HE and safranin O/fast green staining kits were purchased from Solarbio (Beijing, China).HE and safranin O/fast green staining kits were purchased from Solarbio (Beijing, China).

    Article Title: Microglia-specific NLRP3 inhibition mitigates hippocampal neuroinflammation and cognitive deficits after hemorrhagic shock with resuscitation.
    Article Snippet: Mice were euthanized by cervical dislocation if their blood pressure did not return to normal after the transfusion.Mice were euthanized by cervical dislocation if their blood pressure did not return to normal after the transfusion.. 2 μl of MCC950 (10 mg/kg, # HY-12815A/CS-5427, MedChemExpress, Shanghai, China) was administered into the left lateral ventricle (0.5 mm behind the anterior fontanelle, 1 mm left to the median, 2.5 mm below the skull surface) immediately after model establishment (Fig. 1).. ICV injection was chosen to achieve high and consistent brain bioavailability of MCC950, bypassing the blood-brain barrier, which the compound penetrates poorly, and to restrict NLRP3 inhibition primarily to the CNS compartment for mechanistic specificity.ICV injection was chosen to achieve high and consistent brain bioavailability of MCC950, bypassing the blood-brain barrier, which the compound penetrates poorly, and to restrict NLRP3 inhibition primarily to the CNS compartment for mechanistic specificity.

    Negative Control:

    Article Title: A nucleic acid regulation strategy under mechanical stress for intervertebral disc degeneration treatment
    Article Snippet: ption capacity assay of SiNPs with different N/P ratios was evaluated by agarose gel electrophoresis. Besides, HA-F127 SiNPs were characterized by electron microscope and Zeta-Nanosizer. Next, MCC950 (MCE, HY-12815), dissolved in PBS, was combined with HA-F127 SiNPs to achieve a final concentration of 1 mg/mL. Drug release was assessed by monitoring the analyte concentration at 230 nm using HPLC.Th

    Article Title: Engineering biomimetic coronary artery equivalents for Kawasaki disease modeling and drug screening.
    Article Snippet: ATOR and MCC950 were purchased from MedChemExpress (MCE, USA) and dissolved in dimethyl sulfoxide (DMSO) to prepare 10 mM stock solutions.. To determine non-cytotoxic working concentrations and rule out high-dose cytotoxicity, dose-response cell viability assays were first performed.To determine non-cytotoxic working concentrations and rule out high-dose cytotoxicity, dose-response cell viability assays were first performed.

    Article Title: Angelica sinensis polysaccharide inhibits pyroptosis and improves osteoarthritis through the collaborative power of PPARγ and Nrf2
    Article Snippet: It was dissolved in DMSO.It was dissolved in DMSO.. GW1929, NK-252, N-acetylcysteine (NAC), and MCC950 were purchased from MedChemExpress (Shanghai, China) and dissolved in DMSO.. HE and safranin O/fast green staining kits were purchased from Solarbio (Beijing, China).HE and safranin O/fast green staining kits were purchased from Solarbio (Beijing, China).

    Article Title: Microglia-specific NLRP3 inhibition mitigates hippocampal neuroinflammation and cognitive deficits after hemorrhagic shock with resuscitation.
    Article Snippet: Mice were euthanized by cervical dislocation if their blood pressure did not return to normal after the transfusion.Mice were euthanized by cervical dislocation if their blood pressure did not return to normal after the transfusion.. 2 μl of MCC950 (10 mg/kg, # HY-12815A/CS-5427, MedChemExpress, Shanghai, China) was administered into the left lateral ventricle (0.5 mm behind the anterior fontanelle, 1 mm left to the median, 2.5 mm below the skull surface) immediately after model establishment (Fig. 1).. ICV injection was chosen to achieve high and consistent brain bioavailability of MCC950, bypassing the blood-brain barrier, which the compound penetrates poorly, and to restrict NLRP3 inhibition primarily to the CNS compartment for mechanistic specificity.ICV injection was chosen to achieve high and consistent brain bioavailability of MCC950, bypassing the blood-brain barrier, which the compound penetrates poorly, and to restrict NLRP3 inhibition primarily to the CNS compartment for mechanistic specificity.

    Control:

    Article Title: A nucleic acid regulation strategy under mechanical stress for intervertebral disc degeneration treatment
    Article Snippet: ption capacity assay of SiNPs with different N/P ratios was evaluated by agarose gel electrophoresis. Besides, HA-F127 SiNPs were characterized by electron microscope and Zeta-Nanosizer. Next, MCC950 (MCE, HY-12815), dissolved in PBS, was combined with HA-F127 SiNPs to achieve a final concentration of 1 mg/mL. Drug release was assessed by monitoring the analyte concentration at 230 nm using HPLC.Th

    Article Title: Engineering biomimetic coronary artery equivalents for Kawasaki disease modeling and drug screening.
    Article Snippet: ATOR and MCC950 were purchased from MedChemExpress (MCE, USA) and dissolved in dimethyl sulfoxide (DMSO) to prepare 10 mM stock solutions.. To determine non-cytotoxic working concentrations and rule out high-dose cytotoxicity, dose-response cell viability assays were first performed.To determine non-cytotoxic working concentrations and rule out high-dose cytotoxicity, dose-response cell viability assays were first performed.

    Article Title: Angelica sinensis polysaccharide inhibits pyroptosis and improves osteoarthritis through the collaborative power of PPARγ and Nrf2
    Article Snippet: It was dissolved in DMSO.It was dissolved in DMSO.. GW1929, NK-252, N-acetylcysteine (NAC), and MCC950 were purchased from MedChemExpress (Shanghai, China) and dissolved in DMSO.. HE and safranin O/fast green staining kits were purchased from Solarbio (Beijing, China).HE and safranin O/fast green staining kits were purchased from Solarbio (Beijing, China).

    Article Title: Microglia-specific NLRP3 inhibition mitigates hippocampal neuroinflammation and cognitive deficits after hemorrhagic shock with resuscitation.
    Article Snippet: Mice were euthanized by cervical dislocation if their blood pressure did not return to normal after the transfusion.Mice were euthanized by cervical dislocation if their blood pressure did not return to normal after the transfusion.. 2 μl of MCC950 (10 mg/kg, # HY-12815A/CS-5427, MedChemExpress, Shanghai, China) was administered into the left lateral ventricle (0.5 mm behind the anterior fontanelle, 1 mm left to the median, 2.5 mm below the skull surface) immediately after model establishment (Fig. 1).. ICV injection was chosen to achieve high and consistent brain bioavailability of MCC950, bypassing the blood-brain barrier, which the compound penetrates poorly, and to restrict NLRP3 inhibition primarily to the CNS compartment for mechanistic specificity.ICV injection was chosen to achieve high and consistent brain bioavailability of MCC950, bypassing the blood-brain barrier, which the compound penetrates poorly, and to restrict NLRP3 inhibition primarily to the CNS compartment for mechanistic specificity.

    Solvent:

    Article Title: A nucleic acid regulation strategy under mechanical stress for intervertebral disc degeneration treatment
    Article Snippet: ption capacity assay of SiNPs with different N/P ratios was evaluated by agarose gel electrophoresis. Besides, HA-F127 SiNPs were characterized by electron microscope and Zeta-Nanosizer. Next, MCC950 (MCE, HY-12815), dissolved in PBS, was combined with HA-F127 SiNPs to achieve a final concentration of 1 mg/mL. Drug release was assessed by monitoring the analyte concentration at 230 nm using HPLC.Th

    Article Title: Engineering biomimetic coronary artery equivalents for Kawasaki disease modeling and drug screening.
    Article Snippet: ATOR and MCC950 were purchased from MedChemExpress (MCE, USA) and dissolved in dimethyl sulfoxide (DMSO) to prepare 10 mM stock solutions.. To determine non-cytotoxic working concentrations and rule out high-dose cytotoxicity, dose-response cell viability assays were first performed.To determine non-cytotoxic working concentrations and rule out high-dose cytotoxicity, dose-response cell viability assays were first performed.

    Article Title: Angelica sinensis polysaccharide inhibits pyroptosis and improves osteoarthritis through the collaborative power of PPARγ and Nrf2
    Article Snippet: It was dissolved in DMSO.It was dissolved in DMSO.. GW1929, NK-252, N-acetylcysteine (NAC), and MCC950 were purchased from MedChemExpress (Shanghai, China) and dissolved in DMSO.. HE and safranin O/fast green staining kits were purchased from Solarbio (Beijing, China).HE and safranin O/fast green staining kits were purchased from Solarbio (Beijing, China).

    Article Title: Microglia-specific NLRP3 inhibition mitigates hippocampal neuroinflammation and cognitive deficits after hemorrhagic shock with resuscitation.
    Article Snippet: Mice were euthanized by cervical dislocation if their blood pressure did not return to normal after the transfusion.Mice were euthanized by cervical dislocation if their blood pressure did not return to normal after the transfusion.. 2 μl of MCC950 (10 mg/kg, # HY-12815A/CS-5427, MedChemExpress, Shanghai, China) was administered into the left lateral ventricle (0.5 mm behind the anterior fontanelle, 1 mm left to the median, 2.5 mm below the skull surface) immediately after model establishment (Fig. 1).. ICV injection was chosen to achieve high and consistent brain bioavailability of MCC950, bypassing the blood-brain barrier, which the compound penetrates poorly, and to restrict NLRP3 inhibition primarily to the CNS compartment for mechanistic specificity.ICV injection was chosen to achieve high and consistent brain bioavailability of MCC950, bypassing the blood-brain barrier, which the compound penetrates poorly, and to restrict NLRP3 inhibition primarily to the CNS compartment for mechanistic specificity.



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    C1qc mediates the effects of migrasomes on the BBB via the <t>Nlrp3–Caspase</t> 1–GSDMD pathway. (A) Immunoblots revealed C1qc, Nlrp3, cleaved caspase‐1, and GSDMD‐N protein expression levels in C1qc flox/flox model mice injected with AAV–Iba1–Cre or AAV–con ( n = 5). Densitometric quantification of protein levels; the data are presented as the mean SD; ** p < 0.01, *** p < 0.001, **** p < 0.0001. (B) Immunofluorescence staining for GSDMD–N and the endothelial cell marker CD31 in the perihaematomal area of C1qc flox/flox model mice injected with AAV–Iba1–Cre or AAV–con ( n = 5). Normalized quantitative measurement of GSDMD‐N fluorescence intensity relative to CD31 area. The data are presented as the mean ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. (C) Immunofluorescence staining for cleaved caspase‐1 and the endothelial cell marker CD31 in the perihaematomal area of C1qc flox/flox model mice injected with AAV–Iba1–Cre or AAV–con ( n = 5). Fluorescence signal density from cleaved caspase‐1, normalized against the CD31 area, was analysed quantitatively. The data are expressed as ± SD; *** p < 0.001, **** p < 0.0001. (D) GSDMD‐N staining of endothelial cells that were treated as described in Figure ( n = 3). Quantification of the fluorescence intensity of GSDMD‐N of endothelial cells in the vehicle + microglia‐secreting migrasome group, the hemin + microglia‐secreting migrasome group, and the hemin + siC1qc microglia‐secreting migrasome group were also quantified. Data are presented as the mean ± SD with the indicated significance, * p < 0.05, ** p < 0.01, *** p < 0.001. (E) Cleaved caspase‐1 staining of endothelial cells that were treated as described previously in Figure ( n = 3). Quantification of the fluorescence intensity of Cleaved caspase‐1 of endothelial cells in the vehicle + microglial migrasome groups, hemin + microglial migrasome group, and hemin + siC1qc microglial migrasome group were also quantified. The data are presented as the means ± SDs; * p < 0.05, ** p < 0.01. (F) Immunoblots showing the levels of the Nlrp3 and GAPDH proteins in endothelial cells that were treated as described in Figure ( n = 6). Densitometric quantification of protein levels. The data are presented as the mean ± SD; *** p < 0.001. (G) Claudin‐5 staining of endothelial cells that were treated with <t>MCC950</t> and microglial migrasomes without C1qc ( n = 5). Quantification of the fluorescence intensity of Claudin‐5. The data are presented as the mean ± SD; ns, no significant difference; ** p < 0.01, *** p < 0.001. (H) GSDMD‐N immunofluorescence in endothelial cells treated with migrasomes, C1qc‐deficient migrasomes, or C1qc‐deficient migrasomes plus Nigericin. Quantification of the fluorescence intensity of GSDMD‐N. The data are presented as the mean ± SD; ** p < 0.01, *** p < 0.001. (I) Claudin‐5 immunofluorescence in endothelial cells treated with migrasomes, C1qc‐deficient migrasomes, or C1qc‐deficient migrasomes plus Nigericin. Quantification of the fluorescence intensity of Claudin‐5. The data are presented as the mean ± SD; * p < 0.05, *** p < 0.001. (J) Immunoblots showing the levels of the GSDMD‐N, Claudin‐5 and GAPDH proteins in endothelial cells that were treated with migrasomes at different time points ( n = 6). Densitometric quantification of protein levels. The data are presented as the mean ± SD; ns = no significant difference, ** p < 0.01, *** p < 0.001. (K) Cell viability dramatically decreased endothelial cells that were treated with migrasomes at different time points ( n = 3). Data are shown as the means ± SD with significance levels; ns = no significant difference, * p < 0.05, *** p < 0.001, **** p < 0.0001.
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    Pyroptosis execution drives the formation of linear ubiquitin chains (A) Workflow used to induce pyroptosis in THP-1 monocytic cells. (B) Immunoblots of indicated proteins in lysates and supernatants of THP-1 cells primed with 1 μg/mL of LPS for 3 h before treatment with 10 μM of nigericin as indicated. Where indicated, cells were pre-treated with 1 μM <t>of</t> <t>MCC-950</t> for 30 min before nigericin addition. Arrowheads indicate proteins and protein-cleaved fragments. The positions of molecular weights ( M r ) are indicated. (C) Immunoblots of THP-1 treated and analyzed as in (B). (D) Immunoblots of indicated proteins in lysates of A549 cells treated with 10 μM of nigericin. Lysates from untreated THP-1 cells served as a control. Quantification for Met-1 ubiquitination levels over loading (GAPDH). ( n = 3; mean ± SEM; ∗∗ p < 0.01; ANOVA). (E) THP-1 cells stably expressing HMGB1-Lucia deleted with sgRNAs for GSDMD (GSDMD sg ) or their control counterparts (Ctrl sg ) were treated and analyzed as in (B). Sup., supernatants. (F) The release of HMGB1-Lucia was assessed in the supernatants of cells as in (E). ( n = 3; mean ± SEM; ∗∗∗∗ p < 0.0001; t test). All the presented data are representative of at least three independent experiments.
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    Related to Fig. . ( A ) Workflow to induce pyroptosis in monocytic cells. ( B ) THP-1 cells stably expressing HMGB1-Lucia deleted with sgRNAs for CYLD (CYLD sg ), CASP1 (CASP1 sg ), GSDMD (GSDMD sg ), or their control counterparts (Ctrl sg ) were primed with LPS (1 μg/mL) for 3 h before stimulation with Nigericin (10 μM) for 1 h. The release of HMGB1-Lucia was assessed in cell supernatants ( n = 3; mean ± SEM; **** P < 0.0001; two-way ANOVA). P values for each comparison: Ctrl sg vs CYLD sg : P < 0.0001; Ctrl sg vs CASP1 sg : P < 0.0001; Ctrl sg vs GSDMD sg : P < 0.0001. ( C ) Immunoblot analysis of THP-1 cells primed or not with LPS (1 μg/mL) for 3 h before treatment with Nigericin (10 μM) or Raptinal (10 μM) for 1 h. When indicated, cells were pretreated with the NLRP3 inhibitor <t>MCC950</t> (2 μM) for 30 min. Closed and open symbols indicate full-length and cleaved CYLD. ( D ) Immunoblot analysis of Ctrl sg and CASP1 sg THP-1 cells treated as in ( B ). * indicates a nonspecific band. The presented immunoblots are representative of at least three independent experiments. .
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    Image Search Results


    C1qc mediates the effects of migrasomes on the BBB via the Nlrp3–Caspase 1–GSDMD pathway. (A) Immunoblots revealed C1qc, Nlrp3, cleaved caspase‐1, and GSDMD‐N protein expression levels in C1qc flox/flox model mice injected with AAV–Iba1–Cre or AAV–con ( n = 5). Densitometric quantification of protein levels; the data are presented as the mean SD; ** p < 0.01, *** p < 0.001, **** p < 0.0001. (B) Immunofluorescence staining for GSDMD–N and the endothelial cell marker CD31 in the perihaematomal area of C1qc flox/flox model mice injected with AAV–Iba1–Cre or AAV–con ( n = 5). Normalized quantitative measurement of GSDMD‐N fluorescence intensity relative to CD31 area. The data are presented as the mean ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. (C) Immunofluorescence staining for cleaved caspase‐1 and the endothelial cell marker CD31 in the perihaematomal area of C1qc flox/flox model mice injected with AAV–Iba1–Cre or AAV–con ( n = 5). Fluorescence signal density from cleaved caspase‐1, normalized against the CD31 area, was analysed quantitatively. The data are expressed as ± SD; *** p < 0.001, **** p < 0.0001. (D) GSDMD‐N staining of endothelial cells that were treated as described in Figure ( n = 3). Quantification of the fluorescence intensity of GSDMD‐N of endothelial cells in the vehicle + microglia‐secreting migrasome group, the hemin + microglia‐secreting migrasome group, and the hemin + siC1qc microglia‐secreting migrasome group were also quantified. Data are presented as the mean ± SD with the indicated significance, * p < 0.05, ** p < 0.01, *** p < 0.001. (E) Cleaved caspase‐1 staining of endothelial cells that were treated as described previously in Figure ( n = 3). Quantification of the fluorescence intensity of Cleaved caspase‐1 of endothelial cells in the vehicle + microglial migrasome groups, hemin + microglial migrasome group, and hemin + siC1qc microglial migrasome group were also quantified. The data are presented as the means ± SDs; * p < 0.05, ** p < 0.01. (F) Immunoblots showing the levels of the Nlrp3 and GAPDH proteins in endothelial cells that were treated as described in Figure ( n = 6). Densitometric quantification of protein levels. The data are presented as the mean ± SD; *** p < 0.001. (G) Claudin‐5 staining of endothelial cells that were treated with MCC950 and microglial migrasomes without C1qc ( n = 5). Quantification of the fluorescence intensity of Claudin‐5. The data are presented as the mean ± SD; ns, no significant difference; ** p < 0.01, *** p < 0.001. (H) GSDMD‐N immunofluorescence in endothelial cells treated with migrasomes, C1qc‐deficient migrasomes, or C1qc‐deficient migrasomes plus Nigericin. Quantification of the fluorescence intensity of GSDMD‐N. The data are presented as the mean ± SD; ** p < 0.01, *** p < 0.001. (I) Claudin‐5 immunofluorescence in endothelial cells treated with migrasomes, C1qc‐deficient migrasomes, or C1qc‐deficient migrasomes plus Nigericin. Quantification of the fluorescence intensity of Claudin‐5. The data are presented as the mean ± SD; * p < 0.05, *** p < 0.001. (J) Immunoblots showing the levels of the GSDMD‐N, Claudin‐5 and GAPDH proteins in endothelial cells that were treated with migrasomes at different time points ( n = 6). Densitometric quantification of protein levels. The data are presented as the mean ± SD; ns = no significant difference, ** p < 0.01, *** p < 0.001. (K) Cell viability dramatically decreased endothelial cells that were treated with migrasomes at different time points ( n = 3). Data are shown as the means ± SD with significance levels; ns = no significant difference, * p < 0.05, *** p < 0.001, **** p < 0.0001.

    Journal: Journal of Extracellular Vesicles

    Article Title: C1qc Mediates Blood–Brain Barrier Disruption in a Mouse Model of Intracerebral Haemorrhage Through Microglia‐Derived Migrasomes

    doi: 10.1002/jev2.70377

    Figure Lengend Snippet: C1qc mediates the effects of migrasomes on the BBB via the Nlrp3–Caspase 1–GSDMD pathway. (A) Immunoblots revealed C1qc, Nlrp3, cleaved caspase‐1, and GSDMD‐N protein expression levels in C1qc flox/flox model mice injected with AAV–Iba1–Cre or AAV–con ( n = 5). Densitometric quantification of protein levels; the data are presented as the mean SD; ** p < 0.01, *** p < 0.001, **** p < 0.0001. (B) Immunofluorescence staining for GSDMD–N and the endothelial cell marker CD31 in the perihaematomal area of C1qc flox/flox model mice injected with AAV–Iba1–Cre or AAV–con ( n = 5). Normalized quantitative measurement of GSDMD‐N fluorescence intensity relative to CD31 area. The data are presented as the mean ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. (C) Immunofluorescence staining for cleaved caspase‐1 and the endothelial cell marker CD31 in the perihaematomal area of C1qc flox/flox model mice injected with AAV–Iba1–Cre or AAV–con ( n = 5). Fluorescence signal density from cleaved caspase‐1, normalized against the CD31 area, was analysed quantitatively. The data are expressed as ± SD; *** p < 0.001, **** p < 0.0001. (D) GSDMD‐N staining of endothelial cells that were treated as described in Figure ( n = 3). Quantification of the fluorescence intensity of GSDMD‐N of endothelial cells in the vehicle + microglia‐secreting migrasome group, the hemin + microglia‐secreting migrasome group, and the hemin + siC1qc microglia‐secreting migrasome group were also quantified. Data are presented as the mean ± SD with the indicated significance, * p < 0.05, ** p < 0.01, *** p < 0.001. (E) Cleaved caspase‐1 staining of endothelial cells that were treated as described previously in Figure ( n = 3). Quantification of the fluorescence intensity of Cleaved caspase‐1 of endothelial cells in the vehicle + microglial migrasome groups, hemin + microglial migrasome group, and hemin + siC1qc microglial migrasome group were also quantified. The data are presented as the means ± SDs; * p < 0.05, ** p < 0.01. (F) Immunoblots showing the levels of the Nlrp3 and GAPDH proteins in endothelial cells that were treated as described in Figure ( n = 6). Densitometric quantification of protein levels. The data are presented as the mean ± SD; *** p < 0.001. (G) Claudin‐5 staining of endothelial cells that were treated with MCC950 and microglial migrasomes without C1qc ( n = 5). Quantification of the fluorescence intensity of Claudin‐5. The data are presented as the mean ± SD; ns, no significant difference; ** p < 0.01, *** p < 0.001. (H) GSDMD‐N immunofluorescence in endothelial cells treated with migrasomes, C1qc‐deficient migrasomes, or C1qc‐deficient migrasomes plus Nigericin. Quantification of the fluorescence intensity of GSDMD‐N. The data are presented as the mean ± SD; ** p < 0.01, *** p < 0.001. (I) Claudin‐5 immunofluorescence in endothelial cells treated with migrasomes, C1qc‐deficient migrasomes, or C1qc‐deficient migrasomes plus Nigericin. Quantification of the fluorescence intensity of Claudin‐5. The data are presented as the mean ± SD; * p < 0.05, *** p < 0.001. (J) Immunoblots showing the levels of the GSDMD‐N, Claudin‐5 and GAPDH proteins in endothelial cells that were treated with migrasomes at different time points ( n = 6). Densitometric quantification of protein levels. The data are presented as the mean ± SD; ns = no significant difference, ** p < 0.01, *** p < 0.001. (K) Cell viability dramatically decreased endothelial cells that were treated with migrasomes at different time points ( n = 3). Data are shown as the means ± SD with significance levels; ns = no significant difference, * p < 0.05, *** p < 0.001, **** p < 0.0001.

    Article Snippet: Ten milligrams of the inflammasome inhibitor MCC950 (Nlrp3 inhibitor)(Selleck Chemicals, Houston, TX, USA) was dissolved in 1 mL of 1× phosphate‐buffered saline (PBS).

    Techniques: Western Blot, Expressing, Injection, Immunofluorescence, Staining, Marker, Fluorescence

    Pyroptosis execution drives the formation of linear ubiquitin chains (A) Workflow used to induce pyroptosis in THP-1 monocytic cells. (B) Immunoblots of indicated proteins in lysates and supernatants of THP-1 cells primed with 1 μg/mL of LPS for 3 h before treatment with 10 μM of nigericin as indicated. Where indicated, cells were pre-treated with 1 μM of MCC-950 for 30 min before nigericin addition. Arrowheads indicate proteins and protein-cleaved fragments. The positions of molecular weights ( M r ) are indicated. (C) Immunoblots of THP-1 treated and analyzed as in (B). (D) Immunoblots of indicated proteins in lysates of A549 cells treated with 10 μM of nigericin. Lysates from untreated THP-1 cells served as a control. Quantification for Met-1 ubiquitination levels over loading (GAPDH). ( n = 3; mean ± SEM; ∗∗ p < 0.01; ANOVA). (E) THP-1 cells stably expressing HMGB1-Lucia deleted with sgRNAs for GSDMD (GSDMD sg ) or their control counterparts (Ctrl sg ) were treated and analyzed as in (B). Sup., supernatants. (F) The release of HMGB1-Lucia was assessed in the supernatants of cells as in (E). ( n = 3; mean ± SEM; ∗∗∗∗ p < 0.0001; t test). All the presented data are representative of at least three independent experiments.

    Journal: iScience

    Article Title: Linear ubiquitin chain assembly complex contributes to NLRP3-mediated pyroptotic cell death

    doi: 10.1016/j.isci.2026.116448

    Figure Lengend Snippet: Pyroptosis execution drives the formation of linear ubiquitin chains (A) Workflow used to induce pyroptosis in THP-1 monocytic cells. (B) Immunoblots of indicated proteins in lysates and supernatants of THP-1 cells primed with 1 μg/mL of LPS for 3 h before treatment with 10 μM of nigericin as indicated. Where indicated, cells were pre-treated with 1 μM of MCC-950 for 30 min before nigericin addition. Arrowheads indicate proteins and protein-cleaved fragments. The positions of molecular weights ( M r ) are indicated. (C) Immunoblots of THP-1 treated and analyzed as in (B). (D) Immunoblots of indicated proteins in lysates of A549 cells treated with 10 μM of nigericin. Lysates from untreated THP-1 cells served as a control. Quantification for Met-1 ubiquitination levels over loading (GAPDH). ( n = 3; mean ± SEM; ∗∗ p < 0.01; ANOVA). (E) THP-1 cells stably expressing HMGB1-Lucia deleted with sgRNAs for GSDMD (GSDMD sg ) or their control counterparts (Ctrl sg ) were treated and analyzed as in (B). Sup., supernatants. (F) The release of HMGB1-Lucia was assessed in the supernatants of cells as in (E). ( n = 3; mean ± SEM; ∗∗∗∗ p < 0.0001; t test). All the presented data are representative of at least three independent experiments.

    Article Snippet: MCC-950 , Invivogen , Cat#inh-mcc.

    Techniques: Ubiquitin Proteomics, Western Blot, Control, Stable Transfection, Expressing, Luciferase

    LUBAC and linear ubiquitination drive pyroptosis (A) Immunoblots of indicated proteins in lysates and supernatants (Sup.) of THP-1 cells deleted with sgRNAs for HOIP (HOIP sg ) or their control counterparts (Ctrl sg ) primed with 1 μg/mL of LPS for 3 h before treatment with 10 μM of nigericin as indicated. Arrowheads indicate proteins and protein-cleaved fragments. The positions of molecular weights ( M r ) are indicated. (B) THP-1 deleted with sgRNAs for HOIL-1 (HOIL-1 sg ) or their control counterparts (Ctrl sg ) were treated and analyzed as in (A). (C) qPCR analysis of HOIP and HOIL-1 in the indicated THP-1 cell genotypes ( n = 3; mean ± SEM; ∗ p < 0.05; t test). (D) qPCR analysis of pro-IL-1β in the indicated THP-1 cell genotypes primed with 1 μg/mL of LPS for 3 h ( n = 3; mean ± SEM; ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ANOVA). (E) Immunoblots of indicated proteins lysates and supernatants (Sup.) of THP-1 cells treated as in (A). Arrowheads indicate proteins and protein-cleaved fragments. The positions of molecular weights ( M r ) are indicated. (F) PMA-differentiated THP-1 Ctrl sg , HOIP sg , and HOIL-1 sg were stimulated with 10 μM of nigericin in the presence of 100 nM of SYTOX Green. Incucyte acquisition was performed every 30 min to assess green object count ( n = 3; mean ± SEM; ∗∗ p < 0.01, ∗∗∗ p < 0.001 at end time point; ANOVA). (G) THP-1 were primed with LPS for 3 h, pre-treated with 30 μM of HOIPIN-1 or HOIPIN-8 for 30 min, then activated with 10 μM of nigericin as indicated and analyzed as in (A). Arrowheads indicate proteins and protein-cleaved fragments. The positions of molecular weights ( M r ) are indicated. (H) iBMDM cells were treated as in (G). (I) THP-1 cells stably expressing HMGB1-Lucia were treated as in (G). The release of HMGB1-Lucia was assessed in cell supernatants. ( n = 3; mean ± SEM; ∗∗∗∗ p < 0.0001; ANOVA). (J) PMA-differentiated THP-1 were pre-treated with 30 μM of HOIPIN-1, HOIPIN-8, or 1 μM of MCC-950, then treated with 10 μM of nigericin in the presence of 100 nM of SYTOX Green. Incucyte acquisition was performed as in (F) ( n = 3; mean ± SEM; ∗∗ p < 0.01, ∗∗∗∗ p < 0.0001 at end time point; ANOVA). The presented data are representative of at least three independent experiments.

    Journal: iScience

    Article Title: Linear ubiquitin chain assembly complex contributes to NLRP3-mediated pyroptotic cell death

    doi: 10.1016/j.isci.2026.116448

    Figure Lengend Snippet: LUBAC and linear ubiquitination drive pyroptosis (A) Immunoblots of indicated proteins in lysates and supernatants (Sup.) of THP-1 cells deleted with sgRNAs for HOIP (HOIP sg ) or their control counterparts (Ctrl sg ) primed with 1 μg/mL of LPS for 3 h before treatment with 10 μM of nigericin as indicated. Arrowheads indicate proteins and protein-cleaved fragments. The positions of molecular weights ( M r ) are indicated. (B) THP-1 deleted with sgRNAs for HOIL-1 (HOIL-1 sg ) or their control counterparts (Ctrl sg ) were treated and analyzed as in (A). (C) qPCR analysis of HOIP and HOIL-1 in the indicated THP-1 cell genotypes ( n = 3; mean ± SEM; ∗ p < 0.05; t test). (D) qPCR analysis of pro-IL-1β in the indicated THP-1 cell genotypes primed with 1 μg/mL of LPS for 3 h ( n = 3; mean ± SEM; ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ANOVA). (E) Immunoblots of indicated proteins lysates and supernatants (Sup.) of THP-1 cells treated as in (A). Arrowheads indicate proteins and protein-cleaved fragments. The positions of molecular weights ( M r ) are indicated. (F) PMA-differentiated THP-1 Ctrl sg , HOIP sg , and HOIL-1 sg were stimulated with 10 μM of nigericin in the presence of 100 nM of SYTOX Green. Incucyte acquisition was performed every 30 min to assess green object count ( n = 3; mean ± SEM; ∗∗ p < 0.01, ∗∗∗ p < 0.001 at end time point; ANOVA). (G) THP-1 were primed with LPS for 3 h, pre-treated with 30 μM of HOIPIN-1 or HOIPIN-8 for 30 min, then activated with 10 μM of nigericin as indicated and analyzed as in (A). Arrowheads indicate proteins and protein-cleaved fragments. The positions of molecular weights ( M r ) are indicated. (H) iBMDM cells were treated as in (G). (I) THP-1 cells stably expressing HMGB1-Lucia were treated as in (G). The release of HMGB1-Lucia was assessed in cell supernatants. ( n = 3; mean ± SEM; ∗∗∗∗ p < 0.0001; ANOVA). (J) PMA-differentiated THP-1 were pre-treated with 30 μM of HOIPIN-1, HOIPIN-8, or 1 μM of MCC-950, then treated with 10 μM of nigericin in the presence of 100 nM of SYTOX Green. Incucyte acquisition was performed as in (F) ( n = 3; mean ± SEM; ∗∗ p < 0.01, ∗∗∗∗ p < 0.0001 at end time point; ANOVA). The presented data are representative of at least three independent experiments.

    Article Snippet: MCC-950 , Invivogen , Cat#inh-mcc.

    Techniques: Ubiquitin Proteomics, Western Blot, Control, Analysis, Stable Transfection, Expressing, RNA Expression

    Journal: iScience

    Article Title: Linear ubiquitin chain assembly complex contributes to NLRP3-mediated pyroptotic cell death

    doi: 10.1016/j.isci.2026.116448

    Figure Lengend Snippet:

    Article Snippet: MCC-950 , Invivogen , Cat#inh-mcc.

    Techniques: Cell-Signaling, Ubiquitin Proteomics, Recombinant, Electroporation, Protease Inhibitor, Western Blot, Purification, cDNA Synthesis, SYBR Green Assay, Luciferase, LDH Cytotoxicity Assay, Software, Imaging, Multiwell Plates, Optogenetics

    Related to Fig. . ( A ) Workflow to induce pyroptosis in monocytic cells. ( B ) THP-1 cells stably expressing HMGB1-Lucia deleted with sgRNAs for CYLD (CYLD sg ), CASP1 (CASP1 sg ), GSDMD (GSDMD sg ), or their control counterparts (Ctrl sg ) were primed with LPS (1 μg/mL) for 3 h before stimulation with Nigericin (10 μM) for 1 h. The release of HMGB1-Lucia was assessed in cell supernatants ( n = 3; mean ± SEM; **** P < 0.0001; two-way ANOVA). P values for each comparison: Ctrl sg vs CYLD sg : P < 0.0001; Ctrl sg vs CASP1 sg : P < 0.0001; Ctrl sg vs GSDMD sg : P < 0.0001. ( C ) Immunoblot analysis of THP-1 cells primed or not with LPS (1 μg/mL) for 3 h before treatment with Nigericin (10 μM) or Raptinal (10 μM) for 1 h. When indicated, cells were pretreated with the NLRP3 inhibitor MCC950 (2 μM) for 30 min. Closed and open symbols indicate full-length and cleaved CYLD. ( D ) Immunoblot analysis of Ctrl sg and CASP1 sg THP-1 cells treated as in ( B ). * indicates a nonspecific band. The presented immunoblots are representative of at least three independent experiments. .

    Journal: EMBO Reports

    Article Title: Caspase-3 cleaves CYLD to restrict interferon signaling during mitochondrial apoptosis

    doi: 10.1038/s44319-026-00876-4

    Figure Lengend Snippet: Related to Fig. . ( A ) Workflow to induce pyroptosis in monocytic cells. ( B ) THP-1 cells stably expressing HMGB1-Lucia deleted with sgRNAs for CYLD (CYLD sg ), CASP1 (CASP1 sg ), GSDMD (GSDMD sg ), or their control counterparts (Ctrl sg ) were primed with LPS (1 μg/mL) for 3 h before stimulation with Nigericin (10 μM) for 1 h. The release of HMGB1-Lucia was assessed in cell supernatants ( n = 3; mean ± SEM; **** P < 0.0001; two-way ANOVA). P values for each comparison: Ctrl sg vs CYLD sg : P < 0.0001; Ctrl sg vs CASP1 sg : P < 0.0001; Ctrl sg vs GSDMD sg : P < 0.0001. ( C ) Immunoblot analysis of THP-1 cells primed or not with LPS (1 μg/mL) for 3 h before treatment with Nigericin (10 μM) or Raptinal (10 μM) for 1 h. When indicated, cells were pretreated with the NLRP3 inhibitor MCC950 (2 μM) for 30 min. Closed and open symbols indicate full-length and cleaved CYLD. ( D ) Immunoblot analysis of Ctrl sg and CASP1 sg THP-1 cells treated as in ( B ). * indicates a nonspecific band. The presented immunoblots are representative of at least three independent experiments. .

    Article Snippet: LPS (tlrl-peklps), Nigericin (tlrl-nig), and MCC950 (inh-mcc) were obtained from Invivogen.

    Techniques: Stable Transfection, Expressing, Control, Comparison, Western Blot

    Reagents and tools table

    Journal: EMBO Reports

    Article Title: Caspase-3 cleaves CYLD to restrict interferon signaling during mitochondrial apoptosis

    doi: 10.1038/s44319-026-00876-4

    Figure Lengend Snippet: Reagents and tools table

    Article Snippet: LPS (tlrl-peklps), Nigericin (tlrl-nig), and MCC950 (inh-mcc) were obtained from Invivogen.

    Techniques: Ubiquitin Proteomics, Sequencing, Recombinant, Transfection, Software