Journal: The FASEB Journal
Article Title: Surfen-mediated blockade of extratumoral chondroitin sulfate glycosaminoglycans inhibits glioblastoma invasion
doi: 10.1096/fj.201802610RR
Figure Lengend Snippet: Surfen treatment decreases angiogenesis and chemokine signaling in F98 tumors. A) Representative low-magnification epifluorescence imaging of control tumor periphery with insets containing high-magnification confocal images of the nuclear marker DAPI, VEGFa, VEGFR1, and endothelial cell marker Reca. Scale bars, 100 and 50 µm. B) Representative low-magnification epifluorescence imaging of control tumor periphery with insets containing high-magnification confocal images of the nuclear marker DAPI, VEGFa, VEGFR1, and endothelial cell marker Reca. Scale bars, 100 and 50 µm. C) Cell density quantifications of cells positive for either VEGFa or VEGFR1 across control and surfen-treated tumor tissue. D) Colocalization quantification for VEGFa and VEGFR1 between control and surfen-treated tumor tissue. E) Correlation analysis between amounts of VEGFa present and VEGFR1+ cells. F) Representative low-magnification epifluorescence imaging of control tumor periphery with insets containing high-magnification confocal images of the nuclear marker DAPI, chemokine CXCL12, and chemokine receptor CXCR4. Scale bars, 100 and 50 µm. G) Representative low-magnification epifluorescence imaging of surfen-treated tumor boundaries with insets containing high-magnification confocal images of the nuclear marker DAPI, chemokine CXCL12, and chemokine receptor CXCR4. H, I) Quantification for amount of detectable CXCL12 within both control and surfen-treated tissue (H) and for percentage of CXCR4+ cells within the same tissue (I). J) Colocalization analysis for CXCL12 and CXCR4 between control and surfen-treated tumor tissue. Error bars represent means ± sd. *P < 0.05, Student’s t test followed by Mann-Whitney rank-sum test.
Article Snippet: Membranes were then washed with 1× TBS and kept in 1× TBS until imaged using a ChemiDoc MP System (Bio-Rad). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Name Company Catalog no. Reference LAR BD Biosciences (San Jose, CA, USA) 610350 56 , 78 RAGE R&D Systems MAB1179 27 , 79 NG1 Thermo Fisher Scientific PA1-41202 26 NG3 R&D Systems AF2920 26 ERK Cell Signaling Technology (Danvers, MA, USA) 9102S 80 pERK Cell Signaling Technology 9101S 80 Akt Cell Signaling Technology 9272S 81 pAkt Cell Signaling Technology 9271S 81 FAK Thermo Fisher Scientific 44-626G 82 Vinculin MilliporeSigma V9131-100 μl 83 GAPDH Abcam ab8245 84 CD133 Miltenyi Biotec 130-113-108 Human-specific but cross-validated in species CS-56 MilliporeSigma C8035 85 Sox1 Abcam ab87775 86 VEGFa Abcam ab46154 87 VEGFR1 Calbiochem (San Diego, CA, USA) PC322L Human-specific but cross-validated in species RECA-1 Bio-Rad MCA970R 88 CXCL12 R&D Systems MAB350 89 CXCR4 Thermo Fisher Scientific 35-8800 Human-specific but cross-validated in species NeuN MilliporeSigma MAB377 90 Goat anti-mouse IgG1 488 Thermo Fisher Scientific A21121 Open in a separate window NeuN, neuronal nuclei; pAkt, phosphorylated Akt; pERK, phosphorylated ERK; RECA-1, rat endothelial cell antigen antiendothelial cell antibody.
Techniques: Imaging, Marker, MANN-WHITNEY
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