Journal: Stem cell research

Article Title: Non-invasively enhanced intracranial transplantation of mesenchymal stem cells using focused ultrasound mediated by overexpression of cell-adhesion molecules.

doi: 10.1016/j.scr.2020.101726

Figure Lengend Snippet: Fig. 3. CAM co-localization. Co-localization of ICAM-1-positive cells and astrocytes (A), endothelial cells (B), microglia (C) and VCAM-1 cells and endothelial cells (D). Scale bar, 200 μm.

Article Snippet: Sections were blocked with 5% normal goat serum (Vector Labs, Burlingame, CA, USA) and incubated with primary antibodies at the following dilutions: ICAM-1 (sc-8439; Alexa Fluor 488; 1:100; Santa Cruz Biotechnology, Dallas, TX, USA), VCAM-1 (sc-13,160; Alexa Fluor 647; 1:100; Santa Cruz Biotechnology), microglia (Iba1; 019–19,747; 1:300; Wako Chemicals, Richmond, VA, USA), astrocytes (glial fibrillary acidic protein; ab116010; 1:300; Abcam, Cambridge, UK), and endothelial cells (RECA-1; MCA970R; 1:300; Serotec, Oxford, UK).

Techniques:

Journal: Frontiers in Molecular Biosciences

Article Title: Lipid nanoparticles for antisense oligonucleotide gene interference into brain border-associated macrophages

doi: 10.3389/fmolb.2022.887678

Figure Lengend Snippet: Uptake of GR@LNPs by perivascular macrophages (PVMs). Representative immunofluorescences of 6-FAM-labeled GR@LNPs (green in panels C , G , and K ), CD163 + perivascular macrophages (red in panels B , F , and J ), and RECA-1+ endothelium (blue in panels A , E , and I ) in rat prefrontal cortex sections from control rats treated with GR-free@LNP (panels A–D ), animals treated with GR1 and GR2-antisense @LNPs (panels E–H ), and negative control rats treated with GR3-nonsense@LNPs (panels I–L ). Yellow and white head arrows and merge image magnifications, respectively, show apparent (Panel H ) and absent (Panels D,L ) co-localizations of GR@LNPs with perivascular macrophages expressing CD-163 in the different experimental groups studied. Scale bars = 15 μm.

Article Snippet: Then, the sections were incubated 1 h at room temperature with antisera for a mouse antirat RECA-1 antibody (MCA970R, clone HIS52, 1:1000; BioRad ® ).

Techniques: Labeling, Negative Control, Expressing

Journal: Frontiers in Molecular Biosciences

Article Title: Lipid nanoparticles for antisense oligonucleotide gene interference into brain border-associated macrophages

doi: 10.3389/fmolb.2022.887678

Figure Lengend Snippet: Uptake of GR@LNPs by meningeal macrophages (MGMs). Representative immunofluorescences of 6-FAM-labeled GR@LNPs (green in panels C,G, and K ), CD163 + meningeal macrophages (red in panels B,F, and J ), and RECA-1+ endothelium (blue in panels A,E, and I ) in rat prefrontal cortex sections from control rats treated with GR-free@LNP (panels A–D ), animals treated with GR1 and GR2-antisense @LNPs (panels E–H ), and negative control rats treated with GR3-nonsense@LNPs (panels I–L ). Yellow and white head arrows and merge image magnifications, respectively, show apparent (Panel H ), slightly (Panel L ), and absent (Panel D ) co-localizations of GR@LNPs with meningeal macrophages expressing CD-163 in the different experimental groups studied. Scale bars = 15 μm.

Article Snippet: Then, the sections were incubated 1 h at room temperature with antisera for a mouse antirat RECA-1 antibody (MCA970R, clone HIS52, 1:1000; BioRad ® ).

Techniques: Labeling, Negative Control, Expressing