fibroblast antibody er tr7  (Bio-Rad)

Journal: Cell reports

Article Title: Ly6C + monocytes in the skin promote systemic alphavirus dissemination

doi: 10.1016/j.celrep.2024.113876

Figure Lengend Snippet: (Four-week-old male C57BL/6J mice pre-treated (day −1) with anti-GR-1 or isotype control antibody were inoculated in the footpad with 10 3 FFU of CHIKV-LR. (A) DLNs were harvested, and lymphatic fluid was collected. After clarification by centrifugation, a plaque assay was used to quantify infectious virus (n = 6–7, two experiments). (B and C) DLNs were harvested, and tissue homogenates were assessed for infectious virus by plaque assay (B) and viral RNA (C) (n = 6–7, two experiments). (D) Popliteal DLNs were harvested at 18 hpi, fixed, incubated in 30% sucrose for 48 h, cryo-sectioned, and then stained with the following antibodies: anti-CHIKV E1 (CHK-166 N297Q, white), anti-ERTR7 (red), and anti-CD169 (green). Sections were imaged using confocal microscopy and representative DLNs are shown (n = 6, two experiments). Scale bars, 200 μm. Boxed insets denote areas of CHIKV-positive cells. Images are representative of two experiments (n = 6). (E and F) Microscopic images of entire DLN were quantitated using automated image processing software to determine the total volume with detectable CHIKV fluorescence (after anti-CHIKV E1 staining) and the total fluorescent signal per section (n = 6–7, two experiments). Bars indicate median values. Statistical analysis: (A–C, E, and F) Mann-Whitney test, *p < 0.05, **p < 0.01. See also .

Article Snippet: Frozen sections (16 μm thick) were blocked with 5% donkey and bovine serum and then stained with the following Abs: CHK-166 N297Q (anti-E1), – ERTR7 (clone ER-TR7, BioRad, Cat # MCA2402), and CD169 (clone 3D6.112, Biolegend, Cat # 142406).

Techniques: Control, Clarification Assay, Centrifugation, Plaque Assay, Virus, Incubation, Staining, Confocal Microscopy, Software, Fluorescence, MANN-WHITNEY

Journal: Cell reports

Article Title: Ly6C + monocytes in the skin promote systemic alphavirus dissemination

doi: 10.1016/j.celrep.2024.113876

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-mouse ER-TR7 clone ER-TR7 , BioRad , Cat# MCA2402; RRID:AB_915429.

Techniques: Virus, Isolation, Software, Microscopy

Journal: Cell reports

Article Title: Ly6C + monocytes in the skin promote systemic alphavirus dissemination

doi: 10.1016/j.celrep.2024.113876

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-mouse ER-TR7 clone ER-TR7 , BioRad , Cat# MCA2402; RRID:AB_915429.

Techniques: Virus, Isolation, Software, Microscopy

Journal: Cell reports

Article Title: Ly6C + monocytes in the skin promote systemic alphavirus dissemination.

doi: 10.1016/j.celrep.2024.113876

Figure Lengend Snippet: Figure 3. Virus accumulation in the medullary sinus and fluid phase of the DLN is reduced after anti-GR-1 treatment (Four-week-old male C57BL/6J mice pre-treated (day 1) with anti-GR-1 or isotype control antibody were inoculated in the footpad with 103 FFU of CHIKV-LR. (A) DLNs were harvested, and lymphatic fluid was collected. After clarification by centrifugation, a plaque assay was used to quantify infectious virus (n = 6–7, two experiments). (B and C) DLNs were harvested, and tissue homogenates were assessed for infectious virus by plaque assay (B) and viral RNA (C) (n = 6–7, two experiments). (D) Popliteal DLNs were harvested at 18 hpi, fixed, incubated in 30% sucrose for 48 h, cryo-sectioned, and then stained with the following anti- bodies: anti-CHIKV E1 (CHK-166 N297Q, white), anti-ERTR7 (red), and anti-CD169 (green). Sections were imaged using confocal microscopy and representative

Article Snippet: Frozen sections (16 mm thick) were blocked with 5% donkey and bovine serum and then stained with the following Abs: CHK-166 N297Q (anti-E1),55–57 ERTR7 (clone ER-TR7, BioRad, Cat # MCA2402), and CD169 (clone 3D6.112, Biolegend, Cat # 142406).

Techniques: Virus, Control, Centrifugation, Plaque Assay, Incubation, Staining, Confocal Microscopy