ertr7  (Bio-Rad)

Journal: Molecular Neurodegeneration

Article Title: Amyloid-β (Aβ) immunotherapy induced microhemorrhages are linked to vascular inflammation and cerebrovascular damage in a mouse model of Alzheimer’s disease

doi: 10.1186/s13024-024-00758-0

Figure Lengend Snippet: Increased fibrotic response around vascular amyloid deposits in 3D6 Treated PDAPP mice. a Double immunofluorescence of amyloid (X-34, blue) and fibroblasts (ERTR7, red) in PDAPP mice treated with 3D6 or IgG control. X-34 and ERTR7 immunoreactivity overlay (Merge). b Quantification of ERTR7 + area (%) in IgG or 3D6 treated PDAPP mice. Results are shown as mean ± SEM of n = 6 (mice). c Correlation of CD45 and Fibronectin from individual vessels. d Correlation of CD11b and Fibronectin from individual vessels. e Correlation of F480 and Fibronectin from individual vessels. Asterisks indicate significant differences, where * p < 0.05 and **** p < 0.0001 by unpaired Student’s t test. Scale bar 10 μm

Article Snippet: Sections were then incubated overnight at 4 °C with the following antibodies, each diluted 1:100 in blocking solution: anti-Fibrinogen ( PA1-85,429, ThermoFisher), anti-SMA (PA585070, ThermoFisher), anti-CD 31 (14–0311-82, ThermoFisher), anti-VEGF (19,003–1-AP, ThermoFisher), anti-Cldn5 (34–1600, ThermoFisher), anti-GFAP (13–030-0, ThermoFisher), anti-AQP4 (50–173-0968, ThermoFisher), anti-CD19 (14–0194-82, ThermoFisher), anti-CD3 (PIMA514524, Fisher), anti-CD4 (14–9766-82, ThermoFisher), anti-CD8 (14–0808-82, ThermoFisher), anti-Laminin (NB300-144,NovusBio) anti-MHC II (107,610, Citeab), anti-ERTR7 (NB100-64932, Novus) and anti-CD36 (18,836–1-AP, ThermoFisher).

Techniques: Immunofluorescence, Control

Journal: iScience

Article Title: Vhl safeguards thymic epithelial cell identity and thymopoietic capacity by constraining Hif1a activity during development

doi: 10.1016/j.isci.2024.110258

Figure Lengend Snippet:

Article Snippet: anti-ERTR7, unconjugated , Origene , ERTR7; Cat#BM4018.

Techniques: Recombinant, Plasmid Preparation, Reverse Transcription, Staining, Software

Journal: iScience

Article Title: Vhl safeguards thymic epithelial cell identity and thymopoietic capacity by constraining Hif1a activity during development

doi: 10.1016/j.isci.2024.110258

Figure Lengend Snippet:

Article Snippet: anti-ERTR7, unconjugated , Origene , ERTR7; Cat#BM4018.

Techniques: Recombinant, Plasmid Preparation, Reverse Transcription, Staining, Software

Journal: Cell reports

Article Title: Ly6C + monocytes in the skin promote systemic alphavirus dissemination

doi: 10.1016/j.celrep.2024.113876

Figure Lengend Snippet: (Four-week-old male C57BL/6J mice pre-treated (day −1) with anti-GR-1 or isotype control antibody were inoculated in the footpad with 10 3 FFU of CHIKV-LR. (A) DLNs were harvested, and lymphatic fluid was collected. After clarification by centrifugation, a plaque assay was used to quantify infectious virus (n = 6–7, two experiments). (B and C) DLNs were harvested, and tissue homogenates were assessed for infectious virus by plaque assay (B) and viral RNA (C) (n = 6–7, two experiments). (D) Popliteal DLNs were harvested at 18 hpi, fixed, incubated in 30% sucrose for 48 h, cryo-sectioned, and then stained with the following antibodies: anti-CHIKV E1 (CHK-166 N297Q, white), anti-ERTR7 (red), and anti-CD169 (green). Sections were imaged using confocal microscopy and representative DLNs are shown (n = 6, two experiments). Scale bars, 200 μm. Boxed insets denote areas of CHIKV-positive cells. Images are representative of two experiments (n = 6). (E and F) Microscopic images of entire DLN were quantitated using automated image processing software to determine the total volume with detectable CHIKV fluorescence (after anti-CHIKV E1 staining) and the total fluorescent signal per section (n = 6–7, two experiments). Bars indicate median values. Statistical analysis: (A–C, E, and F) Mann-Whitney test, *p < 0.05, **p < 0.01. See also .

Article Snippet: Frozen sections (16 μm thick) were blocked with 5% donkey and bovine serum and then stained with the following Abs: CHK-166 N297Q (anti-E1), – ERTR7 (clone ER-TR7, BioRad, Cat # MCA2402), and CD169 (clone 3D6.112, Biolegend, Cat # 142406).

Techniques: Control, Clarification Assay, Centrifugation, Plaque Assay, Virus, Incubation, Staining, Confocal Microscopy, Software, Fluorescence, MANN-WHITNEY