bio rad mca1957ga rat anti cd45  (Bio-Rad)

Journal: Communications Medicine

Article Title: Rescue of lysosomal acid lipase deficiency in mice by rAAV8 liver gene transfer

doi: 10.1038/s43856-025-00816-8

Figure Lengend Snippet: List of antibodies

Article Snippet: Rat anti-mouse CD68 , Rat , MCA1957GA , Biorad.

Techniques:

Journal: JCI Insight

Article Title: IRF5 governs macrophage adventitial infiltration to fuel abdominal aortic aneurysm formation

doi: 10.1172/jci.insight.171488

Figure Lengend Snippet: ( A ) Representative images of IRF5 and CD68 coimmunostaining in elastase-induced (E-induced) and inactive elastase–induced (IE-induced) AAA samples. Asterisks indicate aortic lumen. Scale bars: 100 μm. ( B and C ) Quantification of IRF5 in mice treated with IE ( n = 7) and E ( n = 7). ( D ) Western blot analysis revealed that IRF5 expression in E-induced AAA tissues was dramatically increased compared with the IE group. Data in B – D are presented as mean ± SD, and significance was determined by unpaired, 2-tailed Student’s t test. *** P < 0.001.

Article Snippet: Anti-CD68 (catalog MCA1957GA) and anti-CD3 antibodies (catalog MCA1477GA) were from Bio-Rad.

Techniques: Western Blot, Expressing

Journal: JCI Insight

Article Title: IRF5 governs macrophage adventitial infiltration to fuel abdominal aortic aneurysm formation

doi: 10.1172/jci.insight.171488

Figure Lengend Snippet: ( A ) Representative images of Irf5 fl/fl and Irf5 ΔMΦ infrarenal abdominal aortas 14 days after treatment with inactive elastase (IE) or elastase (E). The Irf5 fl/fl mice perfused with E showed marked aortic dilation compared with those with IE perfusion. Scale bar: 2 mm. ( B ) Myeloid cell–specific ablation of Irf5 significantly decreased aortic dilation compared with that in Irf5 ΔMΦ mice with E treatment ( n = 7 Irf5 fl/fl mice with IE, n = 7 Irf5 ΔMΦ mice with IE, n = 8 Irf5 fl/fl with E, and n = 8 Irf5 ΔMΦ with E). ( C ) Representative images of immunofluorescent staining of CD68 in AAA tissues from Irf5 fl/fl and Irf5 ΔMΦ mice treated with IE or E for 2 weeks. Asterisks indicate aortic lumen. Scale bar: 100 μm. ( D ) Quantitative analysis of CD68 staining in C . ( E ) Representative flow cytometric analysis of macrophages (CD45 + CD11b + F4/80 + ) in abdominal aortas of Irf5 fl/fl and Irf5 ΔMΦ mice followed by IE or E perfusion for 2 weeks ( n = 6 in each group). Quantification of macrophages by flow cytometry is shown on the right. Data in B , D , and E are presented as mean ± SD, and the significance was determined by 2-way ANOVA followed by Bonferroni’s test. ** P < 0.01, *** P < 0.001.

Article Snippet: Anti-CD68 (catalog MCA1957GA) and anti-CD3 antibodies (catalog MCA1477GA) were from Bio-Rad.

Techniques: Staining, Flow Cytometry

Journal: JCI Insight

Article Title: IRF5 governs macrophage adventitial infiltration to fuel abdominal aortic aneurysm formation

doi: 10.1172/jci.insight.171488

Figure Lengend Snippet: ( A ) Representative images of immunofluorescent staining of PI3Kγ and CD68 in elastase-induced (E-induced) versus inactive elastase–induced (IE-induced) AAA from WT mice. Asterisks indicate aortic lumen. Scale bar: 100 μm. ( B ) Quantification of PI3Kγ in mice treated with IE ( n = 7) or E ( n = 7). ( C ) Western blot analysis suggested that PI3Kγ expression in E-induced AAA tissues was significantly increased compared with the IE group. ( D ) Representative photographs of WT mice and Pik3cg –/– mice subjected to IE or E treatment. Scale bar: 2 mm. ( E ) Pik3cg –/– mice showed a reduced AAA expansion compared with that of WT mice ( n = 7 WT mice with IE, n = 7 Pik3cg –/– mice with IE, n = 7 WT with E, n = 7 Pik3cg –/– with E). ( F ) CD68 immunostaining in AAA tissues from WT mice and Pik3cg –/– mice after E treatment for 2 weeks. Quantification is shown on the right. Asterisks indicate aortic lumen. Scale bar: 100 μm. Data are presented as mean ± SD, and the significance was determined by unpaired, 2-tailed Student’s t test ( B and C ) or 2-way ANOVA followed by Bonferroni’s test ( E and F ). * P < 0.05, *** P < 0.001.

Article Snippet: Anti-CD68 (catalog MCA1957GA) and anti-CD3 antibodies (catalog MCA1477GA) were from Bio-Rad.

Techniques: Staining, Western Blot, Expressing, Immunostaining

Journal: JCI Insight

Article Title: IRF5 governs macrophage adventitial infiltration to fuel abdominal aortic aneurysm formation

doi: 10.1172/jci.insight.171488

Figure Lengend Snippet: ( A ) Representative images of Irf5 fl/fl , Irf5 ΔMΦ , and Pik3cg MΦ+ Irf5 ΔMΦ mice infrarenal abdominal aortas followed by elastase (E) or inactive elastase (IE) perfusion. Scale bar: 2 mm. ( B ) Pik3cg overexpression in myeloid cells dramatically increased aortic dilation compared with that with Irf5 ΔMΦ mice ( n = 7 Irf5 fl/fl mice with IE; n = 6 Irf5 ΔMΦ mice with IE; n = 7 Pik3cg MΦ+ Irf5 ΔMΦ mice with IE; n = 8 Irf5 fl/fl with E; n = 8 Irf5 ΔMΦ with E; n = 7 Pik3cg MΦ+ Irf5 ΔMΦ mice with E). ( C ) Representative images of CD68 immunostaining in AAA tissues from Irf5 fl/fl , Irf5 ΔMΦ , and Pik3cg MΦ+ Irf5 ΔMΦ mice after E treatment for 2 weeks. Asterisks indicate aortic lumen. Scale bar: 100 μm. ( D ) The CD68 staining was quantified. ( E ) Representative flow cytometric analysis of macrophages (CD45 + CD11b + F4/80 + ) in aortas of Irf5 fl/fl , Irf5 ΔMΦ , and Pik3cg MΦ+ Irf5 ΔMΦ mice with AAA establishment ( n = 6 in each group). Quantification of macrophages by flow cytometry is shown on the right. Data are presented as mean ± SD, and significance was determined by 2-way ANOVA followed by Bonferroni’s test ( B ) or 1-way ANOVA followed by Bonferroni’s test ( D and E ). * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: Anti-CD68 (catalog MCA1957GA) and anti-CD3 antibodies (catalog MCA1477GA) were from Bio-Rad.

Techniques: Over Expression, Immunostaining, Staining, Flow Cytometry

Journal: JCI Insight

Article Title: IRF5 governs macrophage adventitial infiltration to fuel abdominal aortic aneurysm formation

doi: 10.1172/jci.insight.171488

Figure Lengend Snippet: ( A ) Representative images of immunofluorescent staining of IRF5 and CD68 in human AAA tissues. In AAA tissues, IRF5 was mostly located in the aortas and present in infiltrated CD68-positive cells. ( B and C ) Quantification of IRF5 in normal aortas ( B , n = 3) and AAA samples ( C , n = 5). ( D ) Representative images of immunofluorescent staining of PI3Kγ and CD68 in human AAA tissues. PI3Kγ was highly expressed in macrophages of human AAA samples. ( E and F ) Quantification of PI3Kγ in normal aortas ( E , n = 3) and AAA samples ( F , n = 5). Scale bars: 100 μm. Data in B , C , E , and F are presented as mean ± SD, and significance was determined by unpaired, 2-tailed Student’s t test. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Anti-CD68 (catalog MCA1957GA) and anti-CD3 antibodies (catalog MCA1477GA) were from Bio-Rad.

Techniques: Staining

Journal: Cell Proliferation

Article Title: Microglial transglutaminase 2 deficiency causes impaired synaptic remodelling and cognitive deficits in mice

doi: 10.1111/cpr.13439

Figure Lengend Snippet: Microglial Tgm2 is required for synapse engulfment in vivo. (A) Representative 3D surface rendering images showing volume reconstruction of IBA1 + microglia, ¹⁰ CD68 + structure (red) and PSD‐95 + puncta (green). Scale bar, 5 μm. (B) Quantification of the number of CD68 + structures per microglia. n = 107–110 microglia from five male mice at 8 weeks old per group. (C) Quantification of the number of PSD‐95 + puncta engulfed within the CD68 + structure per microglia. n = 23–27 microglia from five male mice at 8 weeks old per group. (D) Relative mRNA expression levels of genes related to phagocytosis in hippocampal tissue by the quantitative real‐time PCR analysis. n = 5 animals. Data are presented as mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: For IHC staining, 40‐μm thick brain sections were blocked in blocking solution (2% BSA, 0.3% Triton X‐100 and 0.2% sodium azide) for 1 h at room temperature and incubated overnight with the following primary antibodies: TGM2 (1:500, Abcam, Ab421), IBA1 (1:500, Wako, 019‐19741), PSD‐95 (1:500, ab2723, Abcam), NeuN (1:500 Millipore, ABN78) and/or CD68 (1:500, Bio‐rad, MCA1957GA).

Techniques: In Vivo, Expressing, Real-time Polymerase Chain Reaction