Journal: bioRxiv

Article Title: Knockdown of endogenous circulating C1 inhibitor induces neurovascular impairment, neuroinflammation and cognitive decline

doi: 10.1101/216531

Figure Lengend Snippet: (A) Representative images of the lateral ventricle of CTRL ASO mice stained with CD68 (green), GFAP (red) and DAPI (blue). CD11b cells were positively stained in the lateral ventricle and negatively stained for TMEM119. (B) Representative stitched images of the lateral ventricle in bregma −2.3mm (approximately) stained with CD68 (green) and GFAP (red) between CTRL ASO and C1INH ASO treated mice. (C) Representative images zooming x10 in the lateral ventricle shown in B, showing migrating CD68 positive cells in the lateral ventricle wall area in C1INH ASO treated mice brains. (D) Quantification of CD68 by intensity of staining in CTRL ASO vs. C1INH ASO treated mice brains (100±20.19 vs. 43.94±10.45, p=0.025, n=9). (bar scales are 100μm scale)

Article Snippet: The primary antibodies used were: anti-GFAP (DAKO Z0334); anti-CD11b (Abcam ab-8878); anti-TMEM (Abcam ab209064); anti-PECAM1 (BD Pharmingen 550274); anti-CD68 (AbD Serotec MCA1957GA); anti-CD206 (Thermo PA5-46994); anti-Fibrinogen (Dako A0080); IgG (Thermo scientific); anti-laminin (Fisher scientific RT-795-PO); and anti-iNOS (Abcam ab-129372).

Techniques: Staining

Journal: bioRxiv

Article Title: Knockdown of endogenous circulating C1 inhibitor induces neurovascular impairment, neuroinflammation and cognitive decline

doi: 10.1101/216531

Figure Lengend Snippet: Representative images of GFAP (red) staining and CD68 (green) in the neurovascular units.

Article Snippet: The primary antibodies used were: anti-GFAP (DAKO Z0334); anti-CD11b (Abcam ab-8878); anti-TMEM (Abcam ab209064); anti-PECAM1 (BD Pharmingen 550274); anti-CD68 (AbD Serotec MCA1957GA); anti-CD206 (Thermo PA5-46994); anti-Fibrinogen (Dako A0080); IgG (Thermo scientific); anti-laminin (Fisher scientific RT-795-PO); and anti-iNOS (Abcam ab-129372).

Techniques: Staining

Journal: bioRxiv

Article Title: Knockdown of endogenous circulating C1 inhibitor induces neurovascular impairment, neuroinflammation and cognitive decline

doi: 10.1101/216531

Figure Lengend Snippet: Co-localization of CD68 positive cells and CD206.

Article Snippet: The primary antibodies used were: anti-GFAP (DAKO Z0334); anti-CD11b (Abcam ab-8878); anti-TMEM (Abcam ab209064); anti-PECAM1 (BD Pharmingen 550274); anti-CD68 (AbD Serotec MCA1957GA); anti-CD206 (Thermo PA5-46994); anti-Fibrinogen (Dako A0080); IgG (Thermo scientific); anti-laminin (Fisher scientific RT-795-PO); and anti-iNOS (Abcam ab-129372).

Techniques:

Journal: bioRxiv

Article Title: Knockdown of endogenous circulating C1 inhibitor induces neurovascular impairment, neuroinflammation and cognitive decline

doi: 10.1101/216531

Figure Lengend Snippet: (A) Representative image of the ventricular wall stained with CD68 (green), CD206 (red) and DAPI (blue) showing infiltrating blood derived cells that are not co-localized with CD206 (white arrows) (B) Representative image of positive CD68 cells that are not co-localizing with CD206 in the brain parenchyma. (C) Representative image from bregma −2.54mm of C1INH ASO treated mice brain showing migrating CD68 positive cells in the optic tract region. (D) TMEM119 (red) was negatively stained whereas CD11b (green) was positively stained in the optic tract of C1INH ASO treated mice brain (bar scales are 100μm scale).

Article Snippet: The primary antibodies used were: anti-GFAP (DAKO Z0334); anti-CD11b (Abcam ab-8878); anti-TMEM (Abcam ab209064); anti-PECAM1 (BD Pharmingen 550274); anti-CD68 (AbD Serotec MCA1957GA); anti-CD206 (Thermo PA5-46994); anti-Fibrinogen (Dako A0080); IgG (Thermo scientific); anti-laminin (Fisher scientific RT-795-PO); and anti-iNOS (Abcam ab-129372).

Techniques: Staining, Derivative Assay

Journal: bioRxiv

Article Title: Re-evaluating the Impact of Biological Sex on Atherosclerosis in Apoe −/− and Ldlr −/− mice

doi: 10.1101/2025.09.19.677480

Figure Lengend Snippet: A. Eight-week-old male and female Apoe −/− mice were fed an AMLN diet for 20 weeks to induce atherosclerosis. Fat mass, lean mass and random blood glucose were measured at endpoint (n = 9-10/group). Fat mass and lean mass were analyzed by Mann-Whitney test and random blood glucose was analyzed by unpaired t test. B. Glucose tolerance tests at week 13 (n = 9-10/group). Area under curve (AUC) comparison between sex were performed using Mann-Whitney test. Insulin tolerance tests at week 14 (n = 9-10/group). AUC comparison was analyzed by Mann-Whitney test. C. Serum lipid profiles (TC, TG, LDL, HDL) and Liver TC and TG were measured at endpoint (n = 9-10/group). unpaired t test was used in the data analyzation. D. Blood cell composition was measured at endpoint (n = 8-10/group). Gran% and Lym% were analyzed by unpaired t test. Mon% was analyzed by Mann-Whitney test. E. Aortic en face Oil Red O staining and plaque quantification (n = 9-10/group). Scale bar: 1 mm. unpaired t test was used in the data analyzation. F. Aortic sinus sections Oil Red O staining and plaque area quantification (n = 9-10/group). Scale bar: 250 μm. unpaired t test was used in the data analyzation. HE staining of aortic sinus sections and necrotic core area quantification (n = 7-10/group). Main panel scale bar: 500 μm; inset: 62.5 μm. unpaired t test was used in the data analyzation. Masson’s trichrome staining of aortic sinus sections and collagen content quantification (n = 5/group). Scale bar: 500 μm. unpaired t test was used in the data analyzation. CD68 immunofluorescence staining of aortic sinus sections and macrophage infiltration quantification (n = 9-10/group). Scale bar: 250 μm. Mann-Whitney test was used in the data analyzation. All N numbers given represent biological replicates.

Article Snippet: Primary antibodies against CD68 (BIORAD, MCA1957GA) were diluted and applied to the sections, followed by overnight incubation at 4°C.

Techniques: MANN-WHITNEY, Comparison, Staining, Immunofluorescence

Journal: bioRxiv

Article Title: Re-evaluating the Impact of Biological Sex on Atherosclerosis in Apoe −/− and Ldlr −/− mice

doi: 10.1101/2025.09.19.677480

Figure Lengend Snippet: A.Eight-week-old male and female Ldlr −/− mice were fed an AMLN diet for 20 weeks to induce atherosclerosis. Fat mass, lean mass and random blood glucose were measured at endpoint (n = 10/group). Fat mass and random blood glucose were analyzed by unpaired t test and lean mass was analyzed by Mann-Whitney test. B. Glucose tolerance tests at week 13 (n = 10/group). Area under curve (AUC) comparison between sex were performed using unpaired t test. Insulin tolerance tests at week 14 (n = 9-10/group). AUC comparison was analyzed by Welch’s t test. C. Serum lipid profiles (TC, TG, LDL, HDL) and Liver TC and TG were measured at endpoint (n = 10/group). TC was analyzed by Welch’s t test. TG was analyzed by Mann-Whitney test. LDL, HDL, Liver TC and Liver TG were analyzed by unpaired t test. D. Blood cell composition was measured at endpoint (n = 10/group). Gran% and Lym% were analyzed by Welch’s t test. Mon% was analyzed by Mann-Whitney test. E. Aortic en face Oil Red O staining and plaque quantification (n = 10/group). Scale bar: 1 mm. unpaired t test was used in the data analyzation. F. Aortic sinus sections Oil Red O staining and plaque area quantification (n = 9-10/group). Scale bar: 250 μm. unpaired t test was used in the data analyzation. HE staining of aortic sinus sections and necrotic core area quantification (n = 10/group). Main panel scale bar: 500 μm; inset: 62.5 μm. unpaired t test was used in the data analyzation. Masson’s trichrome staining of aortic sinus sections and collagen content quantification (n = 4-5/group). Scale bar: 500 μm. unpaired t test was used in the data analyzation. CD68 immunofluorescence staining of aortic sinus sections and macrophage infiltration quantification (n = 10/group). Scale bar: 250 μm. unpaired t test was used in the data analyzation. All N numbers given represent biological replicates.

Article Snippet: Primary antibodies against CD68 (BIORAD, MCA1957GA) were diluted and applied to the sections, followed by overnight incubation at 4°C.

Techniques: MANN-WHITNEY, Comparison, Staining, Immunofluorescence