cd163  (Bio-Rad)

Journal: Veterinary Research

Article Title: Protective effect of heat-inactivated Mycobacterium bovis applied intramuscularly is associated with enhanced lung immune response in caprine tuberculosis

doi: 10.1186/s13567-025-01634-5

Figure Lengend Snippet: Quantitative analysis (mean ± standard error) of various immune cells and immunological markers in the lungs of goats immunized and nonimmunized with heat-inactivated M . bovis (HIMB) and exposed to M . caprae . A Immunopositive neutrophils (lysozyme), M2 MΦs (CD163 +), T lymphocytes (CD3+), and B lymphocytes (CD79αcy+). B MΦs expressing iNOS evaluated in lung parenchyma including tuberculous granulomas (Total), and in lung parenchyma without lesions (TBL-). C Immune cells positive to TNFα, IL-1α and IFNγ. D Immune cells positive for IL-6, TGF-β and IL-4. * p ≤ 0.05: Tukey test.

Article Snippet: Anti-human CD163 , M2 MΦs , mAb , 1:200 , TC-autoclave b , Bio-Rad AbD Serotec.

Techniques: Expressing

Journal: Scientific Reports

Article Title: ADAPT-3D:accelerated deep adaptable processing of tissue for 3-dimensional fluorescence tissue imaging for research and clinical settings

doi: 10.1038/s41598-025-16766-z

Figure Lengend Snippet: Effect of ADAPT-3D on finicky antigens and compatibility with deep immunolabeling. A ) Maximum intensity projections of tight junctions (arachnoid barrier: occludin in red and claudin-11 in green, endothelial-cell specific: claudin-5 in grey) found in leptomeninges from a wildtype mouse imaged by confocal microscopy. B ) Extended display showing en face and x-z projections of mouse ileum that was immunolabeled with alpha smooth muscle actin (yellow), lymphatic vasculature (LYVE-1, magenta), myeloid cells (S100A9, cyan), and nuclei (DAPI, grey) followed by imaging with confocal microscopy. C ) Extended display showing en face and x-z projections of ileum from a 16-week-old mouse that expresses TNF ΔARE , a model of ileitis. D ) Extended display showing en face, z-side, and 3D projections of fixed human ileum applied with decolorization, delipidation, immunolabeling with CD163 (green), IBA1 (red), and nuclei (DAPI, grey) followed by refractive index matching.

Article Snippet: 1 cm 3 samples were rinsed in 1X PBS-H for 2 h, incubated in ADAPT:DC for 2 days, ADAPT:PDL for 1 day, followed by ADAPT:BS containing antibodies against CD163 (Clone EDHu-1, Bio-Rad, 1:100), IBA1 (Fujifilm Wako, 019-19741, 1:100), and DAPI (Sigma-Aldrich, D9542, 1:200) for 2 days.

Techniques: Immunolabeling, Confocal Microscopy, Imaging, Refractive Index