Journal: Immunobiology

Article Title: Macrophage-specific nanotechnology-driven CD163 overexpression in human macrophages results in an M2 phenotype under inflammatory conditions.

doi: 10.1016/j.imbio.2017.05.011

Figure Lengend Snippet: Fig. 1. Changes of cytokine concentration in THP-1 macrophages challenged with a single LPS stimulation and a CD163 antibody. Quantification for IL-10 (A), TGF-β (B), IL-1 β (C), TNF- α (D), MCP-1 (E), and IL-6 (F) protein concentration in THP-1 macrophages with a single LPS stimulation and incubated with the anti-CD163 antibody (RM3/1) or its isotype control (Iso) for 24 h (72 h after the addition of LPS). N = 6–10 per group. *P < 0.05, vs. LPS, #P < 0.05, vs. isotype control by Student's t-test.

Article Snippet: Cells were then incubated overnight at 4 °C with a mouse antibody against human CD163 (Serotec, Raleigh, NC, USA, 1:150) and a rabbit antibody against human CD206 (Abcam, Cambridge, MA, USA, 1:250).

Techniques: Concentration Assay, Protein Concentration, Incubation, Control

Journal: Immunobiology

Article Title: Macrophage-specific nanotechnology-driven CD163 overexpression in human macrophages results in an M2 phenotype under inflammatory conditions.

doi: 10.1016/j.imbio.2017.05.011

Figure Lengend Snippet: Fig. 2. Changes of cytokine concentration in primary human macrophages challenged with a single LPS stimulation and a CD163 antibody. Quantification for IL-10 (A), TGF-β (B), IL-1 β (C), TNF-α (D), MCP-1 (E), and IL-6 (F) protein concentration in primary human macrophages with a single LPS stimulation and incubated with the anti-CD163 antibody (RM3/1) or its isotype control (Iso) for 24 h (72 h after the addition of LPS). N = 6–10 per group. #P < 0.05, vs. isotype control by Student's t-test.

Article Snippet: Cells were then incubated overnight at 4 °C with a mouse antibody against human CD163 (Serotec, Raleigh, NC, USA, 1:150) and a rabbit antibody against human CD206 (Abcam, Cambridge, MA, USA, 1:250).

Techniques: Concentration Assay, Protein Concentration, Incubation, Control

Journal: Immunobiology

Article Title: Macrophage-specific nanotechnology-driven CD163 overexpression in human macrophages results in an M2 phenotype under inflammatory conditions.

doi: 10.1016/j.imbio.2017.05.011

Figure Lengend Snippet: Fig. 3. CD163 mRNA induction in THP-1 macrophages challenged with a single LPS stimulation. Quantification for CD163 mRNA induction using Man-PEI complexed with either a plasmid encoding for CD163 gene (pCD163) or the empty vector (pEmpty) in LPS-stimulated THP-1 macrophages at 24, 48, 72 and 96 h after transfection. The expression of CD163 mRNA was normalized to the respective levels of β-actin expression in each group and then calculated as fold change against the expression of the control group (Man-PEI) at each time point. N = 5–6 per group. *P < 0.05 vs. pEmpty by Student's t-test.

Article Snippet: Cells were then incubated overnight at 4 °C with a mouse antibody against human CD163 (Serotec, Raleigh, NC, USA, 1:150) and a rabbit antibody against human CD206 (Abcam, Cambridge, MA, USA, 1:250).

Techniques: Plasmid Preparation, Transfection, Expressing, Control

Journal: Immunobiology

Article Title: Macrophage-specific nanotechnology-driven CD163 overexpression in human macrophages results in an M2 phenotype under inflammatory conditions.

doi: 10.1016/j.imbio.2017.05.011

Figure Lengend Snippet: Fig. 4. CD163 protein induction in THP-1 macrophages challenged with a single LPS stimulation. Microscopic images of nuclear staining using DAPI (blue), CD163 protein (red) and mannose receptor (CD206, green) in LPS-stimulated THP-1 macrophages transfected with Man-PEI complexed with an empty vector (pEmpty), a plasmid encoding for CD163 gene (pCD163) or the Man-PEI nanoparticle alone (A). Quantification of the relative average fluorescence intensity of CD163 (B) or mannose receptor (CD206, C) in LPS-stimulated THP-1 macrophages from 48 to 96 h after transfection. The quantification of the relative average fluorescence intensity was normalized to the respective levels in the Man-PEI group, which was assigned a value equal to 1. N = 3 per group. *P < 0.05 vs. pEmpty by Student's t-test.

Article Snippet: Cells were then incubated overnight at 4 °C with a mouse antibody against human CD163 (Serotec, Raleigh, NC, USA, 1:150) and a rabbit antibody against human CD206 (Abcam, Cambridge, MA, USA, 1:250).

Techniques: Staining, Transfection, Plasmid Preparation

Journal: Immunobiology

Article Title: Macrophage-specific nanotechnology-driven CD163 overexpression in human macrophages results in an M2 phenotype under inflammatory conditions.

doi: 10.1016/j.imbio.2017.05.011

Figure Lengend Snippet: Fig. 5. CD163 protein induction in THP-1 macrophages challenged with a single LPS stimulation. Representative Western blot images of CD163 and Beta-Actin proteins in LPS-stimulated THP-1 macrophages transfected with Man-PEI complexed with an empty vector (pEmp or pEmpty), a plasmid encoding for CD163 gene (pCD163) or the Man-PEI nanoparticle alone (A). Quantification of the relative expression (band density) of CD163 in LPS-stimulated THP-1 macrophages from 48 h after transfection (B). The quantification of the relative density was normalized to the respective levels in the pEmpty group, which was assigned a value equal to 1. N = 10 for pCD163 or pEmpty group. *P < 0.05 vs. pEmpty by Student's t-test.

Article Snippet: Cells were then incubated overnight at 4 °C with a mouse antibody against human CD163 (Serotec, Raleigh, NC, USA, 1:150) and a rabbit antibody against human CD206 (Abcam, Cambridge, MA, USA, 1:250).

Techniques: Western Blot, Transfection, Plasmid Preparation, Expressing

Journal: Immunobiology

Article Title: Macrophage-specific nanotechnology-driven CD163 overexpression in human macrophages results in an M2 phenotype under inflammatory conditions.

doi: 10.1016/j.imbio.2017.05.011

Figure Lengend Snippet: Fig. 6. Changes in cytokine expression in CD163-overexpressing THP-1 macrophages challenged with a single LPS stimulation. Quantification for IL-10 (A), IL-1ra (B), TGF-β (C), TNF-α (D), MCP-1 (E), IL-1β (F), and IL-6 (G) protein concentration in THP-1 macrophages with a single LPS stimulation and transfected with either a plasmid encoding for CD163 gene (pCD163) or the empty vector (pEmpty) at 24, 48, 72 and 96 h after transfection. The concentration of each cytokine/chemokine was normalized to the control group (Man-PEI), which was assigned a value equal to 1. N = 4–9 per group. *P < 0.05, vs. pEmpty by Student's t-test.

Article Snippet: Cells were then incubated overnight at 4 °C with a mouse antibody against human CD163 (Serotec, Raleigh, NC, USA, 1:150) and a rabbit antibody against human CD206 (Abcam, Cambridge, MA, USA, 1:250).

Techniques: Expressing, Protein Concentration, Transfection, Plasmid Preparation, Concentration Assay, Control

Journal: Immunobiology

Article Title: Macrophage-specific nanotechnology-driven CD163 overexpression in human macrophages results in an M2 phenotype under inflammatory conditions.

doi: 10.1016/j.imbio.2017.05.011

Figure Lengend Snippet: Fig. 7. CD163 mRNA induction in THP-1 macrophages challenged with a double LPS stimulation. Quantification for CD163 mRNA using Man-PEI complexed with either a plasmid encoding for CD163 gene (pCD163) or the empty vector (pEmpty) in THP-1 macrophages with a double LPS stimulation at 4 and 24 h after the second stimulus (+4 and +24, respectively). The expression of CD163 mRNA was normalized to the respective levels of β-actin expression in each group and then calculated as fold change against the expression of the control group (Man-PEI) at each time point, which was assigned a value equal to 1. N = 4–6 per group. *P < 0.05 against the pEmpty transfected group by Student's t-test.

Article Snippet: Cells were then incubated overnight at 4 °C with a mouse antibody against human CD163 (Serotec, Raleigh, NC, USA, 1:150) and a rabbit antibody against human CD206 (Abcam, Cambridge, MA, USA, 1:250).

Techniques: Plasmid Preparation, Expressing, Control, Transfection

Journal: Immunobiology

Article Title: Macrophage-specific nanotechnology-driven CD163 overexpression in human macrophages results in an M2 phenotype under inflammatory conditions.

doi: 10.1016/j.imbio.2017.05.011

Figure Lengend Snippet: Fig. 8. Changes of cytokine expression in CD163-overexpressing THP-1 macrophages challenged with a double LPS stimulation. Quantification for IL-10 (A), IL-1ra (B), TGF-β (C), TNF-α (D), MCP-1 (E), IL-1β (F), and IL-6 (G) protein concentration in THP-1 macrophages with a double LPS stimulation and transfected with either a plasmid encoding for CD163 gene (pCD163) or the empty vector (pEmpty) at 4 and 24 h after the second stimulus (+4 and +24, respectively). The concentration of each molecule was normalized to the control group (Man-PEI), which was assigned a value equal to 1. N = 5–13 per group. *P < 0.05, vs. pEmpty by Student's t-test.

Article Snippet: Cells were then incubated overnight at 4 °C with a mouse antibody against human CD163 (Serotec, Raleigh, NC, USA, 1:150) and a rabbit antibody against human CD206 (Abcam, Cambridge, MA, USA, 1:250).

Techniques: Expressing, Protein Concentration, Transfection, Plasmid Preparation, Concentration Assay, Control

Journal: Immunobiology

Article Title: Macrophage-specific nanotechnology-driven CD163 overexpression in human macrophages results in an M2 phenotype under inflammatory conditions.

doi: 10.1016/j.imbio.2017.05.011

Figure Lengend Snippet: Fig. 9. Changes of cytokine expression in CD163-overexpressing THP-1 macrophages challenged with a double LPS stimulation and a CD163 antibody. Quantification for IL-10 (A) and IL- 1ra (B) protein concentration in THP-1 macrophages transfected with either a plasmid encoding for CD163 gene (pCD163) or the empty vector (pEmpty) and incubated with either anti- CD163 antibody (RM3/1) or its isotype control antibody at 4 (for IL-10) and 24 (for IL-1ra) h after a second LPS stimulus. The protein concentration of each group was normalized to the control group (pEmpty), which was assigned a value equal to 1. N = 5–12 per group. *P < 0.05 using Student's t-test.

Article Snippet: Cells were then incubated overnight at 4 °C with a mouse antibody against human CD163 (Serotec, Raleigh, NC, USA, 1:150) and a rabbit antibody against human CD206 (Abcam, Cambridge, MA, USA, 1:250).

Techniques: Expressing, Protein Concentration, Transfection, Plasmid Preparation, Incubation, Control

Journal: Immunobiology

Article Title: Macrophage-specific nanotechnology-driven CD163 overexpression in human macrophages results in an M2 phenotype under inflammatory conditions.

doi: 10.1016/j.imbio.2017.05.011

Figure Lengend Snippet: Fig. 10. CD163 mRNA induction in primary human macrophages challenged with a single LPS stimulation. Quantification for CD163 mRNA induction using Man-PEI complexed with either a plasmid encoding for CD163 gene (pCD163) or the empty vector (pEmpty) in primary human macrophages with a single stimulation of LPS at 48 and 96 h after transfection. The expression of CD163 mRNA was normalized to the respective levels of β-actin expression in each group, and then calculated as fold change against the expression of the pEmpty group at each time point, which was assigned a value equal to 1. N = 6–9 per group. *P < 0.05 against the pEmpty group by Student's t- test.

Article Snippet: Cells were then incubated overnight at 4 °C with a mouse antibody against human CD163 (Serotec, Raleigh, NC, USA, 1:150) and a rabbit antibody against human CD206 (Abcam, Cambridge, MA, USA, 1:250).

Techniques: Plasmid Preparation, Transfection, Expressing

Journal: Immunobiology

Article Title: Macrophage-specific nanotechnology-driven CD163 overexpression in human macrophages results in an M2 phenotype under inflammatory conditions.

doi: 10.1016/j.imbio.2017.05.011

Figure Lengend Snippet: Fig. 11. Changes of cytokine expression in CD163-overexpressing primary human macrophages challenged with a single LPS stimulation. Quantification for IL-10 (A), IL-1ra (B), TGF-β (C), TNF-α (D), MCP-1 (E), IL-1β (F), and IL-6 (G) protein concentration in primary human macrophages with a single LPS stimulation and transfected with either a plasmid encoding for CD163 gene (pCD163) or the empty vector (pEmpty) at 48 and 96 h after transfection. The concentration of each molecule was normalized to the control group (pEmpty), which was assigned a value equal to 1. N = 5–8 per group. *P < 0.05 vs. pEmpty by Student's t-test.

Article Snippet: Cells were then incubated overnight at 4 °C with a mouse antibody against human CD163 (Serotec, Raleigh, NC, USA, 1:150) and a rabbit antibody against human CD206 (Abcam, Cambridge, MA, USA, 1:250).

Techniques: Expressing, Protein Concentration, Transfection, Plasmid Preparation, Concentration Assay, Control

Journal: Cell

Article Title: DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes

doi: 10.1016/j.cell.2017.07.038

Figure Lengend Snippet:

Article Snippet: Horseradish peroxidase goat anti-mouse , Biorad , 170-6516Lab ID #185.

Techniques: Immunofluorescence, Western Blot, Immunoprecipitation, Transduction, Recombinant, Plasmid Preparation, Protease Inhibitor, Multiplex Assay, Mutagenesis, Software, Sterility, Polymer