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hy 103705 matrigel abw  (MedChemExpress)


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    Structured Review

    MedChemExpress hy 103705 matrigel abw
    Hy 103705 Matrigel Abw, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/matrigel/iCRT3/pm42338478-307-216-210
    Average 94 stars, based on 20 article reviews
    hy 103705 matrigel abw - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    MTT Assay:

    Article Title: Methyl Protodioscin Promotes Ferroptosis of Prostate Cancer Cells by Facilitating Dissociation of RB1CC1 from the Detergent-Resistant Membranes and Its Nuclear Translocation.
    Article Snippet: .. The following reagents were used in the study: MPD (Cat#54522-52-0, Chengdu Herbal Biotechnology, Chengdu, China) with a purity of up to 98%; 3-(4,5)-dimethylthiahiazo(-zy1)-3,5-di-phenytetrazoliumromide (MTT) assay reagent (M1020, Solarbio, Beijing, China), BCA protein concentration determination kit (P0012, Beyotime, Shanghai, China), protease inhibitor cocktail (Cat#IKM1010, Solarbio, Beijing, China), 7.5% and 10% rapid gelatin preparation kits (EC0022-A, EC0024-A, SparkJade, Jinan, China), ultrasensitive ECL chemiluminescence kit (ED0015-B, SparkJade, China), radioimmunoprecipitation assay lysate (ED0015-B, SparkJade, China); 4% paraformaldehyde (BL539A, Biosharp, Shanghai, China), Matrigel (HY-K6002, MCE, Shanghai, China), 0.1% crystal violet (G1063, Solarbio), Phostag acrylamide (AAL-107, Wako, Osaka, Japan), glutathione peroxidase 4 inhibitor RSL3 (HY-100218A, MCE, Shanghai, China), iron chelator deferoxamine (DFO; HY-D0903, MCE, Shanghai, China), and JNK inhibitor SP600125 (HY-12041, MCE, Shanghai, China). ..

    Article Title: Methyl Protodioscin Promotes Ferroptosis of Prostate Cancer Cells by Facilitating Dissociation of RB1CC1 from the Detergent-Resistant Membranes and Its Nuclear Translocation
    Article Snippet: .. The following reagents were used in the study: MPD (Cat#54522-52-0, Chengdu Herbal Biotechnology, Chengdu, China) with a purity of up to 98%; 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay reagent (M1020, Solarbio, Beijing, China), BCA protein concentration determination kit (P0012, Beyotime, Shanghai, China), protease inhibitor cocktail (Cat#IKM1010, Solarbio, Beijing, China), 7.5% and 10% rapid gelatin preparation kits (EC0022-A, EC0024-A, SparkJade, Jinan, China), ultrasensitive ECL chemiluminescence kit (ED0015-B, SparkJade, China), radioimmunoprecipitation assay lysate (ED0015-B, SparkJade, China); 4% paraformaldehyde (BL539A, Biosharp, Shanghai, China), Matrigel (HY-K6002, MCE, Shanghai, China), 0.1% crystal violet (G1063, Solarbio), Phos-tag acrylamide (AAL-107, Wako, Osaka, Japan), glutathione peroxidase 4 inhibitor RSL3 (HY-100218A, MCE, Shanghai, China), iron chelator deferoxamine (DFO; HY-D0903, MCE, Shanghai, China), and JNK inhibitor SP600125 (HY-12041, MCE, Shanghai, China). ..

    Protein Concentration:

    Article Title: Methyl Protodioscin Promotes Ferroptosis of Prostate Cancer Cells by Facilitating Dissociation of RB1CC1 from the Detergent-Resistant Membranes and Its Nuclear Translocation.
    Article Snippet: .. The following reagents were used in the study: MPD (Cat#54522-52-0, Chengdu Herbal Biotechnology, Chengdu, China) with a purity of up to 98%; 3-(4,5)-dimethylthiahiazo(-zy1)-3,5-di-phenytetrazoliumromide (MTT) assay reagent (M1020, Solarbio, Beijing, China), BCA protein concentration determination kit (P0012, Beyotime, Shanghai, China), protease inhibitor cocktail (Cat#IKM1010, Solarbio, Beijing, China), 7.5% and 10% rapid gelatin preparation kits (EC0022-A, EC0024-A, SparkJade, Jinan, China), ultrasensitive ECL chemiluminescence kit (ED0015-B, SparkJade, China), radioimmunoprecipitation assay lysate (ED0015-B, SparkJade, China); 4% paraformaldehyde (BL539A, Biosharp, Shanghai, China), Matrigel (HY-K6002, MCE, Shanghai, China), 0.1% crystal violet (G1063, Solarbio), Phostag acrylamide (AAL-107, Wako, Osaka, Japan), glutathione peroxidase 4 inhibitor RSL3 (HY-100218A, MCE, Shanghai, China), iron chelator deferoxamine (DFO; HY-D0903, MCE, Shanghai, China), and JNK inhibitor SP600125 (HY-12041, MCE, Shanghai, China). ..

    Article Title: Methyl Protodioscin Promotes Ferroptosis of Prostate Cancer Cells by Facilitating Dissociation of RB1CC1 from the Detergent-Resistant Membranes and Its Nuclear Translocation
    Article Snippet: .. The following reagents were used in the study: MPD (Cat#54522-52-0, Chengdu Herbal Biotechnology, Chengdu, China) with a purity of up to 98%; 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay reagent (M1020, Solarbio, Beijing, China), BCA protein concentration determination kit (P0012, Beyotime, Shanghai, China), protease inhibitor cocktail (Cat#IKM1010, Solarbio, Beijing, China), 7.5% and 10% rapid gelatin preparation kits (EC0022-A, EC0024-A, SparkJade, Jinan, China), ultrasensitive ECL chemiluminescence kit (ED0015-B, SparkJade, China), radioimmunoprecipitation assay lysate (ED0015-B, SparkJade, China); 4% paraformaldehyde (BL539A, Biosharp, Shanghai, China), Matrigel (HY-K6002, MCE, Shanghai, China), 0.1% crystal violet (G1063, Solarbio), Phos-tag acrylamide (AAL-107, Wako, Osaka, Japan), glutathione peroxidase 4 inhibitor RSL3 (HY-100218A, MCE, Shanghai, China), iron chelator deferoxamine (DFO; HY-D0903, MCE, Shanghai, China), and JNK inhibitor SP600125 (HY-12041, MCE, Shanghai, China). ..

    Protease Inhibitor:

    Article Title: Methyl Protodioscin Promotes Ferroptosis of Prostate Cancer Cells by Facilitating Dissociation of RB1CC1 from the Detergent-Resistant Membranes and Its Nuclear Translocation.
    Article Snippet: .. The following reagents were used in the study: MPD (Cat#54522-52-0, Chengdu Herbal Biotechnology, Chengdu, China) with a purity of up to 98%; 3-(4,5)-dimethylthiahiazo(-zy1)-3,5-di-phenytetrazoliumromide (MTT) assay reagent (M1020, Solarbio, Beijing, China), BCA protein concentration determination kit (P0012, Beyotime, Shanghai, China), protease inhibitor cocktail (Cat#IKM1010, Solarbio, Beijing, China), 7.5% and 10% rapid gelatin preparation kits (EC0022-A, EC0024-A, SparkJade, Jinan, China), ultrasensitive ECL chemiluminescence kit (ED0015-B, SparkJade, China), radioimmunoprecipitation assay lysate (ED0015-B, SparkJade, China); 4% paraformaldehyde (BL539A, Biosharp, Shanghai, China), Matrigel (HY-K6002, MCE, Shanghai, China), 0.1% crystal violet (G1063, Solarbio), Phostag acrylamide (AAL-107, Wako, Osaka, Japan), glutathione peroxidase 4 inhibitor RSL3 (HY-100218A, MCE, Shanghai, China), iron chelator deferoxamine (DFO; HY-D0903, MCE, Shanghai, China), and JNK inhibitor SP600125 (HY-12041, MCE, Shanghai, China). ..

    Article Title: Methyl Protodioscin Promotes Ferroptosis of Prostate Cancer Cells by Facilitating Dissociation of RB1CC1 from the Detergent-Resistant Membranes and Its Nuclear Translocation
    Article Snippet: .. The following reagents were used in the study: MPD (Cat#54522-52-0, Chengdu Herbal Biotechnology, Chengdu, China) with a purity of up to 98%; 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay reagent (M1020, Solarbio, Beijing, China), BCA protein concentration determination kit (P0012, Beyotime, Shanghai, China), protease inhibitor cocktail (Cat#IKM1010, Solarbio, Beijing, China), 7.5% and 10% rapid gelatin preparation kits (EC0022-A, EC0024-A, SparkJade, Jinan, China), ultrasensitive ECL chemiluminescence kit (ED0015-B, SparkJade, China), radioimmunoprecipitation assay lysate (ED0015-B, SparkJade, China); 4% paraformaldehyde (BL539A, Biosharp, Shanghai, China), Matrigel (HY-K6002, MCE, Shanghai, China), 0.1% crystal violet (G1063, Solarbio), Phos-tag acrylamide (AAL-107, Wako, Osaka, Japan), glutathione peroxidase 4 inhibitor RSL3 (HY-100218A, MCE, Shanghai, China), iron chelator deferoxamine (DFO; HY-D0903, MCE, Shanghai, China), and JNK inhibitor SP600125 (HY-12041, MCE, Shanghai, China). ..

    Radio Immunoprecipitation:

    Article Title: Methyl Protodioscin Promotes Ferroptosis of Prostate Cancer Cells by Facilitating Dissociation of RB1CC1 from the Detergent-Resistant Membranes and Its Nuclear Translocation.
    Article Snippet: .. The following reagents were used in the study: MPD (Cat#54522-52-0, Chengdu Herbal Biotechnology, Chengdu, China) with a purity of up to 98%; 3-(4,5)-dimethylthiahiazo(-zy1)-3,5-di-phenytetrazoliumromide (MTT) assay reagent (M1020, Solarbio, Beijing, China), BCA protein concentration determination kit (P0012, Beyotime, Shanghai, China), protease inhibitor cocktail (Cat#IKM1010, Solarbio, Beijing, China), 7.5% and 10% rapid gelatin preparation kits (EC0022-A, EC0024-A, SparkJade, Jinan, China), ultrasensitive ECL chemiluminescence kit (ED0015-B, SparkJade, China), radioimmunoprecipitation assay lysate (ED0015-B, SparkJade, China); 4% paraformaldehyde (BL539A, Biosharp, Shanghai, China), Matrigel (HY-K6002, MCE, Shanghai, China), 0.1% crystal violet (G1063, Solarbio), Phostag acrylamide (AAL-107, Wako, Osaka, Japan), glutathione peroxidase 4 inhibitor RSL3 (HY-100218A, MCE, Shanghai, China), iron chelator deferoxamine (DFO; HY-D0903, MCE, Shanghai, China), and JNK inhibitor SP600125 (HY-12041, MCE, Shanghai, China). ..

    Article Title: Methyl Protodioscin Promotes Ferroptosis of Prostate Cancer Cells by Facilitating Dissociation of RB1CC1 from the Detergent-Resistant Membranes and Its Nuclear Translocation
    Article Snippet: .. The following reagents were used in the study: MPD (Cat#54522-52-0, Chengdu Herbal Biotechnology, Chengdu, China) with a purity of up to 98%; 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay reagent (M1020, Solarbio, Beijing, China), BCA protein concentration determination kit (P0012, Beyotime, Shanghai, China), protease inhibitor cocktail (Cat#IKM1010, Solarbio, Beijing, China), 7.5% and 10% rapid gelatin preparation kits (EC0022-A, EC0024-A, SparkJade, Jinan, China), ultrasensitive ECL chemiluminescence kit (ED0015-B, SparkJade, China), radioimmunoprecipitation assay lysate (ED0015-B, SparkJade, China); 4% paraformaldehyde (BL539A, Biosharp, Shanghai, China), Matrigel (HY-K6002, MCE, Shanghai, China), 0.1% crystal violet (G1063, Solarbio), Phos-tag acrylamide (AAL-107, Wako, Osaka, Japan), glutathione peroxidase 4 inhibitor RSL3 (HY-100218A, MCE, Shanghai, China), iron chelator deferoxamine (DFO; HY-D0903, MCE, Shanghai, China), and JNK inhibitor SP600125 (HY-12041, MCE, Shanghai, China). ..

    Tube Formation Assay:

    Article Title: Sequential hydrogen-release implant for promoting the soft tissue integration through immunomodulatory and pro-remodeling coupling
    Article Snippet: .. Tubule formation assays : A tubule formation assay was conducted using Matrigel (HY-K6001; MCE) to evaluate the vascularization potential. ..

    Cell Culture:

    Article Title: S100B drives glioblastoma invasion and migration through TGF-β2-mediated epithelial-mesenchymal transition
    Article Snippet: Finally, the cells were stained with crystal violet staining solution (cat. no. C0121-100 ml; Beyotime Institute of Biotechnology) for 15 min, and the wells were imaged by full scanning using a microplate reader (Epoch) to count the number of visible colonies (≥30 cells). .. First, NC and shS100B LN229 cells were cultured in DMEM without FBS for 12 h. Subsequently, 0.5×10 5 cells were seeded in the upper chambers of Transwell plates (24 wells; 8-μm pore size; cat. no. 353097; Falcon; Corning, Inc.), which were precoated with Matrigel (cat. no. HY-K6002; MedChemExpress) at 37°C for 30 min for the invasion assay or uncoated for the migration assay. ..

    Pore Size:

    Article Title: S100B drives glioblastoma invasion and migration through TGF-β2-mediated epithelial-mesenchymal transition
    Article Snippet: Finally, the cells were stained with crystal violet staining solution (cat. no. C0121-100 ml; Beyotime Institute of Biotechnology) for 15 min, and the wells were imaged by full scanning using a microplate reader (Epoch) to count the number of visible colonies (≥30 cells). .. First, NC and shS100B LN229 cells were cultured in DMEM without FBS for 12 h. Subsequently, 0.5×10 5 cells were seeded in the upper chambers of Transwell plates (24 wells; 8-μm pore size; cat. no. 353097; Falcon; Corning, Inc.), which were precoated with Matrigel (cat. no. HY-K6002; MedChemExpress) at 37°C for 30 min for the invasion assay or uncoated for the migration assay. ..

    Invasion Assay:

    Article Title: S100B drives glioblastoma invasion and migration through TGF-β2-mediated epithelial-mesenchymal transition
    Article Snippet: Finally, the cells were stained with crystal violet staining solution (cat. no. C0121-100 ml; Beyotime Institute of Biotechnology) for 15 min, and the wells were imaged by full scanning using a microplate reader (Epoch) to count the number of visible colonies (≥30 cells). .. First, NC and shS100B LN229 cells were cultured in DMEM without FBS for 12 h. Subsequently, 0.5×10 5 cells were seeded in the upper chambers of Transwell plates (24 wells; 8-μm pore size; cat. no. 353097; Falcon; Corning, Inc.), which were precoated with Matrigel (cat. no. HY-K6002; MedChemExpress) at 37°C for 30 min for the invasion assay or uncoated for the migration assay. ..

    Migration:

    Article Title: S100B drives glioblastoma invasion and migration through TGF-β2-mediated epithelial-mesenchymal transition
    Article Snippet: Finally, the cells were stained with crystal violet staining solution (cat. no. C0121-100 ml; Beyotime Institute of Biotechnology) for 15 min, and the wells were imaged by full scanning using a microplate reader (Epoch) to count the number of visible colonies (≥30 cells). .. First, NC and shS100B LN229 cells were cultured in DMEM without FBS for 12 h. Subsequently, 0.5×10 5 cells were seeded in the upper chambers of Transwell plates (24 wells; 8-μm pore size; cat. no. 353097; Falcon; Corning, Inc.), which were precoated with Matrigel (cat. no. HY-K6002; MedChemExpress) at 37°C for 30 min for the invasion assay or uncoated for the migration assay. ..

    Cell Differentiation:

    Article Title: Lung endothelial niche signaling governs self-renewal and fate transitions of human alveolar stem cells
    Article Snippet: .. To induce AT2 cell differentiation, 24-well Transwell inserts (Corning, 353097) were coated with 70 μL of 50% Matrigel and allowed to solidify for 30 min. A total of 1 × 10 5 cells were seeded in complete medium onto the inserts, and 700 μL of complete medium supplemented with either DMSO, 10 μM LATS inhibitor (MedChemExpress, HY-138489), 10 nM Trametinib, or a combination of LATS inhibitor and Trametinib was added to the lower chamber. ..



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