Journal: Advanced Healthcare Materials

Article Title: Reinforced Hyaluronic Acid‐Based Matrices Promote 3D Neuronal Network Formation

doi: 10.1002/adhm.202201826

Figure Lengend Snippet: Glycosil supplementation has limited effects on neuronal cell viability. a) Schematic representation of a single 200 µm box of a PCL frame (grey bars) containing Glycosil (grey lines), 3D neurons (green), and supplement (black dots). b) Viability of cortical neurons in Glycosil containing different supplements (lMtg low Matrigel; hMtg high Matrigel; Mxg Maxgel; PG proteoglycan; peptides – RGD, IKVAV, YIGSR; ECMm mixture of various supplements) at DIV1 and 7, compared to viability in Matrigel (marked with #, data from a previous publication [ ^7b ] ). A detailed description of the supplements and their concentrations used is found in Table . Cell viability was quantified by cell counting in five images per experiment. Each grey dot represents one analyzed image. Two or three independent experiments were performed ( n = 2 − 3). Error bars represent SEM values. c) Representative images of selected data are shown in b. Living cells are shown green and dead cells in red. The upper right images represent higher magnifications of cells marked with white boxes. Scale bar: 100 µm. d) Scanning electron microscopy images of PCL‐frames with 200 µm hatch spacing. Upper image: top‐down view; lower image: bottom‐up view. Scale bar 100 µm.

Article Snippet: , Matrigel (734‐0271) , 2.5 μg μL –1 (hMtg); 0.2 μg μL –1 (lMtg) , Corning, NY, USA.

Techniques: Cell Counting, Electron Microscopy

Journal: Advanced Healthcare Materials

Article Title: Reinforced Hyaluronic Acid‐Based Matrices Promote 3D Neuronal Network Formation

doi: 10.1002/adhm.202201826

Figure Lengend Snippet: Neuronal viability in Glycosil remains low in the presence of 2D astrocytes. a) Schematic representation of a single 200 µm box of a PCL frame (grey bars) containing Glycosil (grey lines) and 3D neurons (green), as well as a 2D monolayer of astrocytes and migrating astrocytes (magenta). b) Viability of cortical neurons in Glycosil on an astrocyte monolayer and with or without addition of neurotrophic factors (NTFs; BDNF and CNTF) at DIV1 and 7, compared to viability in pure Matrigel (4.5 mg/ml) with astrocytes or Glycosil supplemented with Matrigel (hMtg). Cell viability was quantified by cell counting in five images per experiment. Grey dots represent the analyzed images. Magenta cell reflects the addition of 2D astrocytes. Two or three independent experiments were performed ( n = 2 − 3). Error bar: SEM. c) Representative images of selected data are shown in b. Living cells are shown green and dead cells in red. White boxes mark the areas shown enlarged in the upper right of the original images. Scale bar:100 µm. d) Immunocytochemical staining of 3D cortical neurons (MAP2, green) and astrocytes (GFAP, magenta) in Glycosil‐PCL composites at DIV7 (nuclei/Hoechst, blue). Scale bar: 50 µm. The temporal color code projection below indicates the location of stained cells in the image stack, given in µm from red 1 µm to blue 23 µm.

Article Snippet: , Matrigel (734‐0271) , 2.5 μg μL –1 (hMtg); 0.2 μg μL –1 (lMtg) , Corning, NY, USA.

Techniques: Cell Counting, Staining