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m3567  (Proteintech)


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    Proteintech m3567
    M3567, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m3567/NONO+Antibody/pm41270757-245-144-150
    Average 95 stars, based on 66 article reviews
    m3567 - by Bioz Stars, 2026-10
    95/100 stars

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    Affinity Purification:

    Article Title: Circadian PERIOD proteins regulate TC-DSB repair through anchoring to the nuclear envelope.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse Anti-53BP1 Monoclonal Antibody, Unconjugated, Clone 19 BD Biosciences Cat# 612522; RRID:AB_2206766 Rabbit Anti-ATM (phospho S1981) antibody [EP1890Y] Abcam Cat# ab81292; RRID:AB_1640207 Rabbit Anti-BMAL1 Polyclonal Antibody, Unconjugated Abcam Cat# ab3350; RRID:AB_303729 Rabbit Anti-CLOCK Polyclonal Antibody, Unconjugated Abcam Cat# ab3517; RRID:AB_303866 Rabbit Anti-Cry1 Polyclonal Antibody, Affinity Purified Bethyl, Discontinued Cat# A302-614A; RRID:AB_10555376 Rabbit Anti-Cryptochrome 2 Polyclonal antibody Proteintech Cat# 13997-1-AP; RRID:AB_10860100 Mouse Anti-DDX5 (p68) Monoclonal Antibody, Unconjugated, Clone 204 Millipore Cat# 05-850; RRID:AB_327052 Rabbit Anti-DDX17 Polyclonal antibody Proteintech Cat# 19910-1-AP; RRID:AB_10667004 Mouse Anti-GAPDH Monoclonal Antibody, 6C5 Sigma-Aldrich Cat# MAB374; RRID:AB_2107445 Rabbit Anti-Histone H2A.X, phospho (Ser139) Monoclonal Antibody, Unconjugated, Clone EP854(2)Y Abcam Cat# ab81299; RRID:AB_1640564 Mouse Anti-phospho-Histone H2A.X (Ser139) Monoclonal Antibody, clone JBW301 Sigma-Aldrich Cat# 05-636 RRID:AB_309864 Rabbit Anti-Lamin B1 Polyclonal antibody - Nuclear Envelope Marker Abcam Cat# ab16048; RRID:AB_443298 Rabbit Anti-Mouse Myosin I beta, Nuclear Antibody, Unconjugated Sigma-Aldrich Cat# M3567; RRID:AB_477180 Rabbit Anti-NONO Polyclonal antibody Proteintech Cat# 11058-1-AP; RRID:AB_2152167 Rabbit Anti-Nup153 Polyclonal antibody Abcam Cat# ab84872; RRID:AB_1859766 Rabbit Phospho-p53 (Ser15) Antibody #9284 Cell Signaling Technology Cat# 9284; RRID:AB_331464 Mouse Anti-PCNA (PC10) Monoclonal Antibody Santa Cruz Biotechnology Cat# sc-56; RRID:AB_628110 Rabbit Anti-PER2 Polyclonal antibody Proteintech, Discontinued Cat# 20359-1-AP; RRID:AB_10733224 Rabbit Anti-PER2 Monoclonal antibody [EPR11381(2)] Abcam, Discontinued Cat# ab179813 Rabbit Anti-RAD51 (H-92) Polyclonal antibody Santa Cruz Biotechnology, Discontinued Cat# sc-8349; RRID:AB_2253533 Goat Anti-RNF4 Polyclonal antibody R and D Systems Cat# AF7964; RRID:AB_3644534 Rabbit Anti-RPA32/RPA2 Polyclonal antibody Abcam Cat# ab10359; RRID:AB_297095 Rabbit Anti-SUN1 Polyclonal antibody Sigma-Aldrich Cat# HPA008346; RRID:AB_1080462 Rabbit Anti-SUN1 Monoclonal antibody [EPR6554] Abcam Cat# ab124770; RRID:AB_10976056 Rabbit Anti-SUN2 Monoclonal antibody [EPR6557] Abcam Cat# ab124916; RRID:AB_10972497 (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 85, 1–18.e1–e11, December 18, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse Anti-alpha-Tubulin Monoclonal Antibody Sigma-Aldrich Cat# T6199; RRID:AB_477583 Goat anti-Rabbit IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Cat# A-11034; RRID:AB_2576217 Goat anti-Rabbit IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa FluorTM 594 Thermo Fisher Scientific Cat# A-11037; RRID:AB_2534095 Goat anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Cat# A-11029; RRID:AB_2534088 Goat anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa FluorTM 594 Thermo Fisher Scientific Cat# A-11032; RRID:AB_2534091 Anti-Mouse IgG (Fc specific)–Peroxidase antibody produced in goat Sigma-Aldrich Cat# A2554; RRID:AB_258008 Anti-Rabbit IgG (whole molecule)Peroxidase antibody produced in goat Sigma-Aldrich Cat# A0545; RRID:AB_257896 Chemicals, peptides, and recombinant proteins 4-hydroxytamoxifen (OHT) Sigma-Aldrich Cat# H7904 Auxin: Indole-3-acetic acid sodium salt (IAA) Sigma-Aldrich Cat# I5148 etoposide Sigma-Aldrich Cat# E1383 doxorubicin Sigma-Aldrich Cat# D1515 ATM inhibitor (ATMi) KU-55933 Sigma-Aldrich Cat# SML1109 ATR inhibitor (ATRi) ETP-46464 Sigma-Aldrich Cat# SML1321 DNA-PK inhibitor (DNA-PKi) NU-7441 Selleckchem Cat# S2638 dexamethasone (Dex) Sigma-Aldrich Cat# D4902 Citifluor Clinisciences Cat# AF1-25 EdU (5-éthynyl-2’-désoxyuridine) Thermo Fisher Scientific Cat# A10044 Hoechst 33342 Thermo Fisher Scientific Cat# 62249 AMV reverse transcriptase Promega Cat# M510F Crystal Violet Solution Sigma-Aldrich Cat# V5265 TB GreenTM Premix Ex TaqTM (Tli RNaseH Plus) Takara Cat# RR420L PrimeSTAR Max DNA Polymerase Takara Cat# R045A Nuclease Benzonase Sigma-Aldrich Cat# E1014 RNAseH New England Biolabs Cat# M0297 AsiSI New England Biolabs Cat# R0630 BanI New England Biolabs Cat# R0118 BanII New England Biolabs Cat# R0119 cOmpleteTM Protease Inhibitor Cocktail Roche Cat# 11 836 153 001 Phosphatase Inhibitor Cocktail 2 Sigma-Aldrich Cat# P5726 Critical commercial assays Kit de prolifération cellulaire EdU Click-iTTM pour imagerie, colorant Alexa FluorTM 647 Thermo Fisher Scientific Cat# C10340 NEBuilder HiFi DNA Assembly Cloning Kit New England Biolabs Cat# E5520S QIAprep Spin Miniprep Kit Qiagen Cat# 27104 EndoFree Plasmid Maxi Kit Qiagen Cat# 12362 DNeasy Blood & Tissue Kit Qiagen Cat# 69504 RNeasy Mini Kit Qiagen Cat# 74104 (Continued on next page) ll OPEN ACCESS Article e2 Molecular Cell 85, 1–18.e1–e11, December 18, 2025

    Marker:

    Article Title: Circadian PERIOD proteins regulate TC-DSB repair through anchoring to the nuclear envelope.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse Anti-53BP1 Monoclonal Antibody, Unconjugated, Clone 19 BD Biosciences Cat# 612522; RRID:AB_2206766 Rabbit Anti-ATM (phospho S1981) antibody [EP1890Y] Abcam Cat# ab81292; RRID:AB_1640207 Rabbit Anti-BMAL1 Polyclonal Antibody, Unconjugated Abcam Cat# ab3350; RRID:AB_303729 Rabbit Anti-CLOCK Polyclonal Antibody, Unconjugated Abcam Cat# ab3517; RRID:AB_303866 Rabbit Anti-Cry1 Polyclonal Antibody, Affinity Purified Bethyl, Discontinued Cat# A302-614A; RRID:AB_10555376 Rabbit Anti-Cryptochrome 2 Polyclonal antibody Proteintech Cat# 13997-1-AP; RRID:AB_10860100 Mouse Anti-DDX5 (p68) Monoclonal Antibody, Unconjugated, Clone 204 Millipore Cat# 05-850; RRID:AB_327052 Rabbit Anti-DDX17 Polyclonal antibody Proteintech Cat# 19910-1-AP; RRID:AB_10667004 Mouse Anti-GAPDH Monoclonal Antibody, 6C5 Sigma-Aldrich Cat# MAB374; RRID:AB_2107445 Rabbit Anti-Histone H2A.X, phospho (Ser139) Monoclonal Antibody, Unconjugated, Clone EP854(2)Y Abcam Cat# ab81299; RRID:AB_1640564 Mouse Anti-phospho-Histone H2A.X (Ser139) Monoclonal Antibody, clone JBW301 Sigma-Aldrich Cat# 05-636 RRID:AB_309864 Rabbit Anti-Lamin B1 Polyclonal antibody - Nuclear Envelope Marker Abcam Cat# ab16048; RRID:AB_443298 Rabbit Anti-Mouse Myosin I beta, Nuclear Antibody, Unconjugated Sigma-Aldrich Cat# M3567; RRID:AB_477180 Rabbit Anti-NONO Polyclonal antibody Proteintech Cat# 11058-1-AP; RRID:AB_2152167 Rabbit Anti-Nup153 Polyclonal antibody Abcam Cat# ab84872; RRID:AB_1859766 Rabbit Phospho-p53 (Ser15) Antibody #9284 Cell Signaling Technology Cat# 9284; RRID:AB_331464 Mouse Anti-PCNA (PC10) Monoclonal Antibody Santa Cruz Biotechnology Cat# sc-56; RRID:AB_628110 Rabbit Anti-PER2 Polyclonal antibody Proteintech, Discontinued Cat# 20359-1-AP; RRID:AB_10733224 Rabbit Anti-PER2 Monoclonal antibody [EPR11381(2)] Abcam, Discontinued Cat# ab179813 Rabbit Anti-RAD51 (H-92) Polyclonal antibody Santa Cruz Biotechnology, Discontinued Cat# sc-8349; RRID:AB_2253533 Goat Anti-RNF4 Polyclonal antibody R and D Systems Cat# AF7964; RRID:AB_3644534 Rabbit Anti-RPA32/RPA2 Polyclonal antibody Abcam Cat# ab10359; RRID:AB_297095 Rabbit Anti-SUN1 Polyclonal antibody Sigma-Aldrich Cat# HPA008346; RRID:AB_1080462 Rabbit Anti-SUN1 Monoclonal antibody [EPR6554] Abcam Cat# ab124770; RRID:AB_10976056 Rabbit Anti-SUN2 Monoclonal antibody [EPR6557] Abcam Cat# ab124916; RRID:AB_10972497 (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 85, 1–18.e1–e11, December 18, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse Anti-alpha-Tubulin Monoclonal Antibody Sigma-Aldrich Cat# T6199; RRID:AB_477583 Goat anti-Rabbit IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Cat# A-11034; RRID:AB_2576217 Goat anti-Rabbit IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa FluorTM 594 Thermo Fisher Scientific Cat# A-11037; RRID:AB_2534095 Goat anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Cat# A-11029; RRID:AB_2534088 Goat anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa FluorTM 594 Thermo Fisher Scientific Cat# A-11032; RRID:AB_2534091 Anti-Mouse IgG (Fc specific)–Peroxidase antibody produced in goat Sigma-Aldrich Cat# A2554; RRID:AB_258008 Anti-Rabbit IgG (whole molecule)Peroxidase antibody produced in goat Sigma-Aldrich Cat# A0545; RRID:AB_257896 Chemicals, peptides, and recombinant proteins 4-hydroxytamoxifen (OHT) Sigma-Aldrich Cat# H7904 Auxin: Indole-3-acetic acid sodium salt (IAA) Sigma-Aldrich Cat# I5148 etoposide Sigma-Aldrich Cat# E1383 doxorubicin Sigma-Aldrich Cat# D1515 ATM inhibitor (ATMi) KU-55933 Sigma-Aldrich Cat# SML1109 ATR inhibitor (ATRi) ETP-46464 Sigma-Aldrich Cat# SML1321 DNA-PK inhibitor (DNA-PKi) NU-7441 Selleckchem Cat# S2638 dexamethasone (Dex) Sigma-Aldrich Cat# D4902 Citifluor Clinisciences Cat# AF1-25 EdU (5-éthynyl-2’-désoxyuridine) Thermo Fisher Scientific Cat# A10044 Hoechst 33342 Thermo Fisher Scientific Cat# 62249 AMV reverse transcriptase Promega Cat# M510F Crystal Violet Solution Sigma-Aldrich Cat# V5265 TB GreenTM Premix Ex TaqTM (Tli RNaseH Plus) Takara Cat# RR420L PrimeSTAR Max DNA Polymerase Takara Cat# R045A Nuclease Benzonase Sigma-Aldrich Cat# E1014 RNAseH New England Biolabs Cat# M0297 AsiSI New England Biolabs Cat# R0630 BanI New England Biolabs Cat# R0118 BanII New England Biolabs Cat# R0119 cOmpleteTM Protease Inhibitor Cocktail Roche Cat# 11 836 153 001 Phosphatase Inhibitor Cocktail 2 Sigma-Aldrich Cat# P5726 Critical commercial assays Kit de prolifération cellulaire EdU Click-iTTM pour imagerie, colorant Alexa FluorTM 647 Thermo Fisher Scientific Cat# C10340 NEBuilder HiFi DNA Assembly Cloning Kit New England Biolabs Cat# E5520S QIAprep Spin Miniprep Kit Qiagen Cat# 27104 EndoFree Plasmid Maxi Kit Qiagen Cat# 12362 DNeasy Blood & Tissue Kit Qiagen Cat# 69504 RNeasy Mini Kit Qiagen Cat# 74104 (Continued on next page) ll OPEN ACCESS Article e2 Molecular Cell 85, 1–18.e1–e11, December 18, 2025



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    Image Search Results


    Known β-actin-interacting proteins co-purified with ERα by IgG-Sepharose affinity chromatography and identified by nano-LC-MS/MS analysis The list of β-actin-interacting proteins from the UniHI database was crossed with the list of proteins co-purified with TAP-ERα, but not with the TAP tag alone, and identified by nano-LC-MS/MS analysis of purified samples. Proteins are listed with information relative to the MS data that led to their identification. Two identified proteins not listed as validated direct interactors of actin in the UniHI database and ERα itself are reported in bold. n/a, not applicable.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Identification of a Hormone-regulated Dynamic Nuclear Actin Network Associated with Estrogen Receptor ? in Human Breast Cancer Cell Nuclei *

    doi: 10.1074/mcp.M900519-MCP200

    Figure Lengend Snippet: Known β-actin-interacting proteins co-purified with ERα by IgG-Sepharose affinity chromatography and identified by nano-LC-MS/MS analysis The list of β-actin-interacting proteins from the UniHI database was crossed with the list of proteins co-purified with TAP-ERα, but not with the TAP tag alone, and identified by nano-LC-MS/MS analysis of purified samples. Proteins are listed with information relative to the MS data that led to their identification. Two identified proteins not listed as validated direct interactors of actin in the UniHI database and ERα itself are reported in bold. n/a, not applicable.

    Article Snippet: Membranes were blocked with 5% nonfat dry milk in TBST buffer (0.01 m Tris-HCl, pH 8.0, 0.15 m NaCl, and 0.1% Tween 20) and incubated with the following Abs: rabbit polyclonal anti-ERα (sc-543), -Arp2 (sc-15389), -Arp3 (sc-15390), and -lamin B (sc-6216) from Santa Cruz Biotechnology; mouse monoclonal anti-β-actin (A1978) and -α-tubulin (T6199), rabbit polyclonal anti-myosin 1c (nuclear isoform, M3567), and mouse monoclonal anti-FLAG M2 (F1804) from Sigma-Aldrich; rabbit polyclonal anti-TAP (CAB1001) from Open Biosystems; mouse monoclonal anti-flightless I (ab28089) and -gelsolin (ab11081), rabbit monoclonal anti-nucleophosmin (ab52644), and rabbit polyclonal anti-DDX5 (ab21696) from Abcam; and mouse monoclonal anti-RNA polymerase II (MMS-126R) from Covance.

    Techniques: Affinity Chromatography, Purification, Sequencing