Review



lscm  (Nikon)


Bioz Verified Symbol Nikon is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    Nikon lscm
    Hypotonic treatment optimization for enhanced intracellular visualization. (A) Optical micrographs of cells treated with three hypotonic solutions prepared by mixing distilled water (DW) and PBS—pure DW (0X, 100% DW), 0.2X PBS solution, and 0.5X PBS solution. Cells were imaged at 1, 5, 8 and 10 min post-treatment. White arrows indicate representative cells showing membrane rupture or cellular stress under stronger hypotonic conditions (0X and 0.2X PBS). Scale bar: 50 μm. (B) <t>Confocal</t> <t>microscopy</t> images of bEnd.3 cells after hypotonic treatment showing cell morphology with DAPI (blue) and cell mask staining. Left panel: 1X PBS control treatment for 10 min. Right panel: 0.5X PBS treatment for 10 min. Scale bar: 10 μm. (C) Quantitative analysis of cell height measured using NIS-E software program following 10 min treatment with 1X PBS (control) versus 0.5X PBS hypotonic solution. Data are presented as mean ± SEM ( n = 13).
    Lscm, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 57094 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lscm/product/Nikon
    Average 99 stars, based on 57094 article reviews
    lscm - by Bioz Stars, 2026-06
    99/100 stars

    Images

    1) Product Images from "Cell swelling and upright mounting-based imaging for high-resolution visualization of intracellular trafficking across the BBB using conventional confocal microscopy"

    Article Title: Cell swelling and upright mounting-based imaging for high-resolution visualization of intracellular trafficking across the BBB using conventional confocal microscopy

    Journal: Drug Delivery

    doi: 10.1080/10717544.2025.2608235

    Hypotonic treatment optimization for enhanced intracellular visualization. (A) Optical micrographs of cells treated with three hypotonic solutions prepared by mixing distilled water (DW) and PBS—pure DW (0X, 100% DW), 0.2X PBS solution, and 0.5X PBS solution. Cells were imaged at 1, 5, 8 and 10 min post-treatment. White arrows indicate representative cells showing membrane rupture or cellular stress under stronger hypotonic conditions (0X and 0.2X PBS). Scale bar: 50 μm. (B) Confocal microscopy images of bEnd.3 cells after hypotonic treatment showing cell morphology with DAPI (blue) and cell mask staining. Left panel: 1X PBS control treatment for 10 min. Right panel: 0.5X PBS treatment for 10 min. Scale bar: 10 μm. (C) Quantitative analysis of cell height measured using NIS-E software program following 10 min treatment with 1X PBS (control) versus 0.5X PBS hypotonic solution. Data are presented as mean ± SEM ( n = 13).
    Figure Legend Snippet: Hypotonic treatment optimization for enhanced intracellular visualization. (A) Optical micrographs of cells treated with three hypotonic solutions prepared by mixing distilled water (DW) and PBS—pure DW (0X, 100% DW), 0.2X PBS solution, and 0.5X PBS solution. Cells were imaged at 1, 5, 8 and 10 min post-treatment. White arrows indicate representative cells showing membrane rupture or cellular stress under stronger hypotonic conditions (0X and 0.2X PBS). Scale bar: 50 μm. (B) Confocal microscopy images of bEnd.3 cells after hypotonic treatment showing cell morphology with DAPI (blue) and cell mask staining. Left panel: 1X PBS control treatment for 10 min. Right panel: 0.5X PBS treatment for 10 min. Scale bar: 10 μm. (C) Quantitative analysis of cell height measured using NIS-E software program following 10 min treatment with 1X PBS (control) versus 0.5X PBS hypotonic solution. Data are presented as mean ± SEM ( n = 13).

    Techniques Used: Membrane, Confocal Microscopy, Staining, Control, Software

    Confocal microscopy analysis of early endosome trafficking patterns. (A) Cells treated with A647-Tf for 5 min, 15 min, or 1 h, stained with DAPI (blue), Rab5 (green, upper panels), and EEA1 (green, lower panels). (B) Cells treated with A647-anti-TfR Ab under the same conditions and staining. The red signal corresponds to internalized A647-Tf or A647-anti-TfR Ab. Scale bar: 10 μm.
    Figure Legend Snippet: Confocal microscopy analysis of early endosome trafficking patterns. (A) Cells treated with A647-Tf for 5 min, 15 min, or 1 h, stained with DAPI (blue), Rab5 (green, upper panels), and EEA1 (green, lower panels). (B) Cells treated with A647-anti-TfR Ab under the same conditions and staining. The red signal corresponds to internalized A647-Tf or A647-anti-TfR Ab. Scale bar: 10 μm.

    Techniques Used: Confocal Microscopy, Staining



    Similar Products

    fv3000rs lscm  (Beyotime)
    99
    Beyotime fv3000rs lscm
    Fv3000rs Lscm, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fv3000rs lscm/product/Beyotime
    Average 99 stars, based on 1 article reviews
    fv3000rs lscm - by Bioz Stars, 2026-06
    99/100 stars
    		  Buy from Supplier
    laser scanning confocal microscope lscm multiphoton fixed cell imaging olympus fv 3000 model  (Olympus)
    99
    Olympus laser scanning confocal microscope lscm multiphoton fixed cell imaging olympus fv 3000 model
    Laser Scanning Confocal Microscope Lscm Multiphoton Fixed Cell Imaging Olympus Fv 3000 Model, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/laser scanning confocal microscope lscm multiphoton fixed cell imaging olympus fv 3000 model/product/Olympus
    Average 99 stars, based on 1 article reviews
    laser scanning confocal microscope lscm multiphoton fixed cell imaging olympus fv 3000 model - by Bioz Stars, 2026-06
    99/100 stars
    		  Buy from Supplier
    lscm  (Olympus)
    99
    Olympus lscm
    Lscm, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lscm/product/Olympus
    Average 99 stars, based on 1 article reviews
    lscm - by Bioz Stars, 2026-06
    99/100 stars
    		  Buy from Supplier
    laser scanning confocal microscope lscm  (Olympus)
    99
    Olympus laser scanning confocal microscope lscm
    Laser Scanning Confocal Microscope Lscm, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/laser scanning confocal microscope lscm/product/Olympus
    Average 99 stars, based on 1 article reviews
    laser scanning confocal microscope lscm - by Bioz Stars, 2026-06
    99/100 stars
    		  Buy from Supplier
    lscm  (Nikon)
    99
    Nikon lscm
    Hypotonic treatment optimization for enhanced intracellular visualization. (A) Optical micrographs of cells treated with three hypotonic solutions prepared by mixing distilled water (DW) and PBS—pure DW (0X, 100% DW), 0.2X PBS solution, and 0.5X PBS solution. Cells were imaged at 1, 5, 8 and 10 min post-treatment. White arrows indicate representative cells showing membrane rupture or cellular stress under stronger hypotonic conditions (0X and 0.2X PBS). Scale bar: 50 μm. (B) <t>Confocal</t> <t>microscopy</t> images of bEnd.3 cells after hypotonic treatment showing cell morphology with DAPI (blue) and cell mask staining. Left panel: 1X PBS control treatment for 10 min. Right panel: 0.5X PBS treatment for 10 min. Scale bar: 10 μm. (C) Quantitative analysis of cell height measured using NIS-E software program following 10 min treatment with 1X PBS (control) versus 0.5X PBS hypotonic solution. Data are presented as mean ± SEM ( n = 13).
    Lscm, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lscm/product/Nikon
    Average 99 stars, based on 1 article reviews
    lscm - by Bioz Stars, 2026-06
    99/100 stars
    		  Buy from Supplier
    stellaris 8 lscm microscope  (Danaher Inc)
    99
    Danaher Inc stellaris 8 lscm microscope
    Hypotonic treatment optimization for enhanced intracellular visualization. (A) Optical micrographs of cells treated with three hypotonic solutions prepared by mixing distilled water (DW) and PBS—pure DW (0X, 100% DW), 0.2X PBS solution, and 0.5X PBS solution. Cells were imaged at 1, 5, 8 and 10 min post-treatment. White arrows indicate representative cells showing membrane rupture or cellular stress under stronger hypotonic conditions (0X and 0.2X PBS). Scale bar: 50 μm. (B) <t>Confocal</t> <t>microscopy</t> images of bEnd.3 cells after hypotonic treatment showing cell morphology with DAPI (blue) and cell mask staining. Left panel: 1X PBS control treatment for 10 min. Right panel: 0.5X PBS treatment for 10 min. Scale bar: 10 μm. (C) Quantitative analysis of cell height measured using NIS-E software program following 10 min treatment with 1X PBS (control) versus 0.5X PBS hypotonic solution. Data are presented as mean ± SEM ( n = 13).
    Stellaris 8 Lscm Microscope, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stellaris 8 lscm microscope/product/Danaher Inc
    Average 99 stars, based on 1 article reviews
    stellaris 8 lscm microscope - by Bioz Stars, 2026-06
    99/100 stars
    		  Buy from Supplier
    nikon c1 lscm camera  (Nikon)
    99
    Nikon nikon c1 lscm camera
    Hypotonic treatment optimization for enhanced intracellular visualization. (A) Optical micrographs of cells treated with three hypotonic solutions prepared by mixing distilled water (DW) and PBS—pure DW (0X, 100% DW), 0.2X PBS solution, and 0.5X PBS solution. Cells were imaged at 1, 5, 8 and 10 min post-treatment. White arrows indicate representative cells showing membrane rupture or cellular stress under stronger hypotonic conditions (0X and 0.2X PBS). Scale bar: 50 μm. (B) <t>Confocal</t> <t>microscopy</t> images of bEnd.3 cells after hypotonic treatment showing cell morphology with DAPI (blue) and cell mask staining. Left panel: 1X PBS control treatment for 10 min. Right panel: 0.5X PBS treatment for 10 min. Scale bar: 10 μm. (C) Quantitative analysis of cell height measured using NIS-E software program following 10 min treatment with 1X PBS (control) versus 0.5X PBS hypotonic solution. Data are presented as mean ± SEM ( n = 13).
    Nikon C1 Lscm Camera, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nikon c1 lscm camera/product/Nikon
    Average 99 stars, based on 1 article reviews
    nikon c1 lscm camera - by Bioz Stars, 2026-06
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Hypotonic treatment optimization for enhanced intracellular visualization. (A) Optical micrographs of cells treated with three hypotonic solutions prepared by mixing distilled water (DW) and PBS—pure DW (0X, 100% DW), 0.2X PBS solution, and 0.5X PBS solution. Cells were imaged at 1, 5, 8 and 10 min post-treatment. White arrows indicate representative cells showing membrane rupture or cellular stress under stronger hypotonic conditions (0X and 0.2X PBS). Scale bar: 50 μm. (B) Confocal microscopy images of bEnd.3 cells after hypotonic treatment showing cell morphology with DAPI (blue) and cell mask staining. Left panel: 1X PBS control treatment for 10 min. Right panel: 0.5X PBS treatment for 10 min. Scale bar: 10 μm. (C) Quantitative analysis of cell height measured using NIS-E software program following 10 min treatment with 1X PBS (control) versus 0.5X PBS hypotonic solution. Data are presented as mean ± SEM ( n = 13).

    Journal: Drug Delivery

    Article Title: Cell swelling and upright mounting-based imaging for high-resolution visualization of intracellular trafficking across the BBB using conventional confocal microscopy

    doi: 10.1080/10717544.2025.2608235

    Figure Lengend Snippet: Hypotonic treatment optimization for enhanced intracellular visualization. (A) Optical micrographs of cells treated with three hypotonic solutions prepared by mixing distilled water (DW) and PBS—pure DW (0X, 100% DW), 0.2X PBS solution, and 0.5X PBS solution. Cells were imaged at 1, 5, 8 and 10 min post-treatment. White arrows indicate representative cells showing membrane rupture or cellular stress under stronger hypotonic conditions (0X and 0.2X PBS). Scale bar: 50 μm. (B) Confocal microscopy images of bEnd.3 cells after hypotonic treatment showing cell morphology with DAPI (blue) and cell mask staining. Left panel: 1X PBS control treatment for 10 min. Right panel: 0.5X PBS treatment for 10 min. Scale bar: 10 μm. (C) Quantitative analysis of cell height measured using NIS-E software program following 10 min treatment with 1X PBS (control) versus 0.5X PBS hypotonic solution. Data are presented as mean ± SEM ( n = 13).

    Article Snippet: High-resolution apicobasal imaging was performed using LSCM (Nikon, A1Plus, Tokyo, Japan) equipped with a 60 × oil immersion objective lens (Apo 60 × oil λS DIC N2, numerical aperture = 1.40).

    Techniques: Membrane, Confocal Microscopy, Staining, Control, Software

    Confocal microscopy analysis of early endosome trafficking patterns. (A) Cells treated with A647-Tf for 5 min, 15 min, or 1 h, stained with DAPI (blue), Rab5 (green, upper panels), and EEA1 (green, lower panels). (B) Cells treated with A647-anti-TfR Ab under the same conditions and staining. The red signal corresponds to internalized A647-Tf or A647-anti-TfR Ab. Scale bar: 10 μm.

    Journal: Drug Delivery

    Article Title: Cell swelling and upright mounting-based imaging for high-resolution visualization of intracellular trafficking across the BBB using conventional confocal microscopy

    doi: 10.1080/10717544.2025.2608235

    Figure Lengend Snippet: Confocal microscopy analysis of early endosome trafficking patterns. (A) Cells treated with A647-Tf for 5 min, 15 min, or 1 h, stained with DAPI (blue), Rab5 (green, upper panels), and EEA1 (green, lower panels). (B) Cells treated with A647-anti-TfR Ab under the same conditions and staining. The red signal corresponds to internalized A647-Tf or A647-anti-TfR Ab. Scale bar: 10 μm.

    Article Snippet: High-resolution apicobasal imaging was performed using LSCM (Nikon, A1Plus, Tokyo, Japan) equipped with a 60 × oil immersion objective lens (Apo 60 × oil λS DIC N2, numerical aperture = 1.40).

    Techniques: Confocal Microscopy, Staining