lovo cell line (ATCC)
Structured Review

Lovo Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 2930 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lovo cell line/product/ATCC
Average 97 stars, based on 2930 article reviews
Images
1) Product Images from "FAM65A, as a potential predictor of prognosis, promotes colorectal cancer progression via activating Ras/ERK/RSK signaling"
Article Title: FAM65A, as a potential predictor of prognosis, promotes colorectal cancer progression via activating Ras/ERK/RSK signaling
Journal: iScience
doi: 10.1016/j.isci.2026.114662
Figure Legend Snippet: FAM65A promotes CRC cell proliferation and inhibits cell apoptosis (A) Western blot analysis FAM65A expression in human normal colon cells and CRC cells was assessed. (B) Western blot analysis FAM65A knockdown efficiency in SW480 and LOVO cells. (C) CCK8 assay was performed with shCtrl and shFAM65A in SW480 and LOVO cell lines, n = 3, ∗∗∗ p < 0.001. (D) The outcomes of the colony formation assay for shCtrl and shFAM65A in SW480 and LOVO cells were reported. (E) A quantitative analysis of the colony formation assay was conducted, n = 6, ∗ p < 0.05, ∗∗ p < 0.01. (F) The results of the EdU assay for shCtrl and shFAM65A in SW480 and LOVO cells were presented, where EdU-positive stained cells exhibited green fluorescence and nuclei displayed blue fluorescence. Scale bars, 100 μm. (G) A quantitative analysis of the EdU assay results was performed, n = 3, ∗∗∗ p < 0.001. (H) The results of the apoptosis assay for shCtrl and shFAM65A in SW480 and LOVO cells were documented. Scale bars, 50 μm. (I) A quantitative analysis of the apoptosis assay was conducted, n = 3, ∗∗ p < 0.01, ∗∗∗ p < 0.001. (J) The western blot results for the expression of Ki-67, cleaved caspase 3, Bcl-2, and Bax in SW480 and LOVO cells for both shCtrl and shFAM65A were presented. Data are presented as mean ± SEM of biologically independent experiments.
Techniques Used: Western Blot, Expressing, Knockdown, CCK-8 Assay, Colony Assay, EdU Assay, Staining, Fluorescence, Apoptosis Assay
Figure Legend Snippet: FAM65A promotes CRC cell migration in vitro (A) Transwell migration assay was conducted to evaluate the migration capabilities of shCtrl and shFAM65A in SW480 and LOVO cell lines. Scale bars, 50 μm. (B) A quantitative analysis of the Transwell migration assay was performed, n = 3, ∗∗∗ p < 0.001. (C) The results of wound healing assay comparing shCtrl and shFAM65A in SW480 and LOVO cells were obtained. Scale bars, 50 μm. (D) A quantitative analysis of the wound healing assay was also conducted, n = 3, ∗∗∗ p < 0.001. (E) Western blot analysis was performed to assess the expression levels of EMT markers in SW480 and LOVO cells. Data are presented as mean ± SEM of biologically independent experiments.
Techniques Used: Migration, In Vitro, Transwell Migration Assay, Wound Healing Assay, Western Blot, Expressing