Journal: Molecules

Article Title: Multifaceted Anticancer Activity of Flavanone/Chromanone Intermediates for Five-Membered Heterocyclic Derivatives: Targeting Oxidative Stress, Apoptosis, and MAPK Signaling in Colorectal Cancer

doi: 10.3390/molecules31030534

Figure Lengend Snippet: Measurement of PARP degradation in HCT 116, SW620, LoVo, Caco-2 and HT-29 cells exposed to compound 1 , 3 and 5 . Cells were incubated with derivatives for 24 h at concentrations of the calculated IC 50 . The results represent mean ± SD of the data from 3 individual experiments, untreated control cells arbitrarily taken as 100%, * p < 0.05 vs. control.

Article Snippet: The study incorporated a total of 5 human adherent colon cancer lines: HCT 116 (ATCC ® CCL-247 TM ; colorectal cancer, Dukes’ type A), SW620 (ATCC ® CCL-227 TM ; colorectal adenocancer, Dukes’ type C), LoVo (ATCC ® CCL-229 TM , colorectal adenocancer, Dukes’ type C, grade IV), Caco-2 (ATCC ® HTB-37 TM , colorectal adenocancer, Dukes’ type B) and HT-29 (ATCC ® HTB-38 TM , colorectal adenocancer, Dukes’ type C).

Techniques: Incubation, Control

Journal: Molecules

Article Title: Multifaceted Anticancer Activity of Flavanone/Chromanone Intermediates for Five-Membered Heterocyclic Derivatives: Targeting Oxidative Stress, Apoptosis, and MAPK Signaling in Colorectal Cancer

doi: 10.3390/molecules31030534

Figure Lengend Snippet: ( a ) Fluorescence anisotropy of DAUDA and TMA-DPH label in HCT 116, SW620, LoVo, Caco-2 and HT-29 cells exposed to compound 1 , 3 and 5 . Results represent mean ± SD of data from three individual experiments, each performed with at least six repeats; * p < 0.05 versus control. ( b ) Fluorescence anisotropy of DAUDA and TMA-DPH label in HCT 116, SW620, LoVo, Caco-2 and HT-29 cells exposed to compound 1 , 3 and 5 after 1 h preincubation with NAC. Results represent mean ± SD of data from three individual experiments, each performed with at least six repeats; * p < 0.05 versus control.

Article Snippet: The study incorporated a total of 5 human adherent colon cancer lines: HCT 116 (ATCC ® CCL-247 TM ; colorectal cancer, Dukes’ type A), SW620 (ATCC ® CCL-227 TM ; colorectal adenocancer, Dukes’ type C), LoVo (ATCC ® CCL-229 TM , colorectal adenocancer, Dukes’ type C, grade IV), Caco-2 (ATCC ® HTB-37 TM , colorectal adenocancer, Dukes’ type B) and HT-29 (ATCC ® HTB-38 TM , colorectal adenocancer, Dukes’ type C).

Techniques: Fluorescence, Control

Journal: Molecules

Article Title: Multifaceted Anticancer Activity of Flavanone/Chromanone Intermediates for Five-Membered Heterocyclic Derivatives: Targeting Oxidative Stress, Apoptosis, and MAPK Signaling in Colorectal Cancer

doi: 10.3390/molecules31030534

Figure Lengend Snippet: Measurement of caspase-9 activity in HCT 116, SW620, LoVo, Caco-2 and HT-29 cells exposed to compound 1 , 3 and 5 . Cells were incubated with derivatives for 24 h at concentrations of the calculated IC 50 . The results represent mean ± SD of the data from two individual experiments, untreated control cells arbitrarily taken as 100%, * p < 0.05 vs. control.

Article Snippet: The study incorporated a total of 5 human adherent colon cancer lines: HCT 116 (ATCC ® CCL-247 TM ; colorectal cancer, Dukes’ type A), SW620 (ATCC ® CCL-227 TM ; colorectal adenocancer, Dukes’ type C), LoVo (ATCC ® CCL-229 TM , colorectal adenocancer, Dukes’ type C, grade IV), Caco-2 (ATCC ® HTB-37 TM , colorectal adenocancer, Dukes’ type B) and HT-29 (ATCC ® HTB-38 TM , colorectal adenocancer, Dukes’ type C).

Techniques: Activity Assay, Incubation, Control

Journal: Molecules

Article Title: Multifaceted Anticancer Activity of Flavanone/Chromanone Intermediates for Five-Membered Heterocyclic Derivatives: Targeting Oxidative Stress, Apoptosis, and MAPK Signaling in Colorectal Cancer

doi: 10.3390/molecules31030534

Figure Lengend Snippet: Measurement of caspase-3 activity in HCT 116, SW620, LoVo, Caco-2 and HT-29 cells exposed to compound 1 , 3 and 5 . Cells were incubated with derivatives for 24 h at concentrations of the calculated IC 50 . The results represent mean ± SD of the data from two individual experiments, untreated control cells arbitrarily taken as 100%, * p < 0.05 vs. control.

Article Snippet: The study incorporated a total of 5 human adherent colon cancer lines: HCT 116 (ATCC ® CCL-247 TM ; colorectal cancer, Dukes’ type A), SW620 (ATCC ® CCL-227 TM ; colorectal adenocancer, Dukes’ type C), LoVo (ATCC ® CCL-229 TM , colorectal adenocancer, Dukes’ type C, grade IV), Caco-2 (ATCC ® HTB-37 TM , colorectal adenocancer, Dukes’ type B) and HT-29 (ATCC ® HTB-38 TM , colorectal adenocancer, Dukes’ type C).

Techniques: Activity Assay, Incubation, Control

Journal: Molecules

Article Title: Multifaceted Anticancer Activity of Flavanone/Chromanone Intermediates for Five-Membered Heterocyclic Derivatives: Targeting Oxidative Stress, Apoptosis, and MAPK Signaling in Colorectal Cancer

doi: 10.3390/molecules31030534

Figure Lengend Snippet: ( a ) Measurement of phosphorylated kinase ERK 1/2 activity in HCT 116, SW620, LoVo, Caco-2 and HT-29 cells exposed to compound 1 , 3 and 5 . Cells were incubated with derivatives for 24 h at concentrations of the calculated IC 50 . The results represent mean ± SD of the data from 2 individual experiments, untreated control cells arbitrarily taken as 100%, * p < 0.05 vs. control. ( b ) Measurement of phosphorylated kinase p38 activity in HCT 116, SW620, LoVo, Caco-2 and HT-29 cells exposed to compound 1 , 3 and 5 . Cells were incubated with derivatives for 24 h at concentrations of the calculated IC 50 . The results represent mean ± SD of the data from two individual experiments, untreated control cells arbitrarily taken as 100%, * p < 0.05 vs. control. ( c ) Measurement of phosphorylated kinase p38 activity in HCT 116, SW620, LoVo, Caco-2 and HT-29 cells exposed to compound 1 , 3 and 5. Cells were incubated with derivatives for 24 h at concentrations of the calculated IC 50 . The results represent mean ± SD of the data from two individual experiments, untreated control cells arbitrarily taken as 100%, * p < 0.05 vs. control.

Article Snippet: The study incorporated a total of 5 human adherent colon cancer lines: HCT 116 (ATCC ® CCL-247 TM ; colorectal cancer, Dukes’ type A), SW620 (ATCC ® CCL-227 TM ; colorectal adenocancer, Dukes’ type C), LoVo (ATCC ® CCL-229 TM , colorectal adenocancer, Dukes’ type C, grade IV), Caco-2 (ATCC ® HTB-37 TM , colorectal adenocancer, Dukes’ type B) and HT-29 (ATCC ® HTB-38 TM , colorectal adenocancer, Dukes’ type C).

Techniques: Activity Assay, Incubation, Control

Journal: Molecules

Article Title: Multifaceted Anticancer Activity of Flavanone/Chromanone Intermediates for Five-Membered Heterocyclic Derivatives: Targeting Oxidative Stress, Apoptosis, and MAPK Signaling in Colorectal Cancer

doi: 10.3390/molecules31030534

Figure Lengend Snippet: Measurement of Nrf2 activity in HCT 116, SW620, LoVo, Caco-2 and HT-29 cells exposed to compound 1 , 3 and 5 . Cells were incubated with derivatives for 24 h at concentrations of the calculated IC 50 . The results represent mean ± SD of the data from 2 individual experiments, untreated control cells arbitrarily taken as 100%, * p < 0.05 vs. control.

Article Snippet: The study incorporated a total of 5 human adherent colon cancer lines: HCT 116 (ATCC ® CCL-247 TM ; colorectal cancer, Dukes’ type A), SW620 (ATCC ® CCL-227 TM ; colorectal adenocancer, Dukes’ type C), LoVo (ATCC ® CCL-229 TM , colorectal adenocancer, Dukes’ type C, grade IV), Caco-2 (ATCC ® HTB-37 TM , colorectal adenocancer, Dukes’ type B) and HT-29 (ATCC ® HTB-38 TM , colorectal adenocancer, Dukes’ type C).

Techniques: Activity Assay, Incubation, Control

Journal: JCI Insight

Article Title: LIN28B induces a differentiation program through CDX2 in colon cancer

doi: 10.1172/jci.insight.140382

Figure Lengend Snippet: ( A ) Left: subcutaneous xenograft experiments with LoVo cells ( n = 6 per cell type) showed a significant increase in tumor weight with CDX2 KD with sh-CDX2 no. 1 (472.38 ± 126.23 mg at euthanization) or sh-CDX2 no. 2 (353.38 ± 112.90 mg at euthanization) as compared with control cells (123.88 ± 48.86 mg at euthanization). Right: the images of tumors. Scale bars: 10 mm. ( B ) Ki-67 staining in the subcutaneous xenograft tumor of LoVo cells with control or CDX2 KD, representative images (upper) and the quantification (lower). Scale bars: 100 μm. n = 3. ( C ) Transwell chamber invasion assay of LoVo cells with control or CDX2 KD, representative images (upper) and the quantification (lower). Scale bars: 100 μm. n = 3. ( D ) Ki-67 staining in the liver metastatic tumor of LoVo cells with control or CDX2 KD, representative images (upper) and the quantification (lower). Scale bars: 100 μm. n = 3. ( E ) Epithelial-mesenchymal transition (EMT) marker expression (qPCR) in LoVo control/CDX2 KD cells ( n = 3). ( F ) Representative IHC of CDX2, E-cadherin, and CK20 in the liver metastatic tumor of LoVo cells with control or CDX2 KD. Scale bars: 100 μm. Data are presented as mean ± SEM. Unpaired, 2-tailed Student’s t test ( D ) and 1-way ANOVA followed by Dunnett’s multiple-comparison test as post hoc analysis ( A , B , C , and E ) were performed. * P < 0.05, ** P < 0.01.

Article Snippet: AMACR overexpression in Caco-2 cells and LoVo cells was performed using Genecopoeia overexpression system with EX-Z1680-Lv151 (AMACR o/e) and EX-NEG-Lv151 (empty vector) constructs.

Techniques: Control, Staining, Invasion Assay, Marker, Expressing, Comparison

Journal: Bone Marrow Transplantation

Article Title: Short treatment of peripheral blood cells product with Fas ligand using closed automated cell processing system significantly reduces immune cell reactivity of the graft in vitro and in vivo

doi: 10.1038/s41409-022-01698-3

Figure Lengend Snippet: a FasL AM MPBCs treatment process performed using the Fresenius Kabi LOVO functionally closed automated cell processing system, designed for HSCT. MPBCs graft characterization following ( b – f ) 100 ng/ml FasL AM treatment or ( g – k ) 400 ng/ml FasL AM treatment. Percentage of ( b , g ) CD3 + and ( d , i ) CD34 + cells per total CD45 + population. Percentage of annexin V positive ( c , h ) CD3 + and ( e , j ) CD34 + cells per 7AAD − population. f , k Percentage of multi potent stem and progenitor cells (CD34 + CD38 neg/low ) per total CD34 + 7AAD − population. b – e : N = 16, f : N = 9, g – k : N = 3. Mean ± SEM, * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001; Paired T -test.

Article Snippet: Fig. 1 FasL AM MPBCs treatment significantly reduces CD3 + cells without compromising the quantity and quality of CD34 + cells. a FasL AM MPBCs treatment process performed using the Fresenius Kabi LOVO functionally closed automated cell processing system, designed for HSCT.

Techniques:

Journal: International Journal of Nanomedicine

Article Title: Synergistic effect of a combination of nanoparticulate Fe 3 O 4 and gambogic acid on phosphatidylinositol 3-kinase/Akt/Bad pathway of LOVO cells

doi: 10.2147/IJN.S32475

Figure Lengend Snippet: ( A ) Inhibition rate of LOVO cells treated with various concentrations of GA for different times. ( B ) Inhibition rate of LOVO cells treated with the combination of GA and Fe 3 O 4 magnetic nanoparticles for 48 hours. Notes: * P < 0.05 when compared with controls; # P > 0.05 when compared with the group treated with Fe 3 O 4 magnetic nanoparticles. Abbreviations: MNPs-Fe 3 O 4 , magnetic nanoparticles of Fe 3 O 4 ; GA, gambogic acid.

Article Snippet: A human colorectal cancer cell line (LOVO) purchased from Nanjing Keygen Biotech Co, Ltd was cultured in RPMI 1640 medium containing 10% (v/v) heat-inactivated new-born calf serum, penicillin G 100 U/mL, and streptomycin 100 μg/mL at 37°C in a humidified environment containing 5% CO 2 .

Techniques: Inhibition

Journal: International Journal of Nanomedicine

Article Title: Synergistic effect of a combination of nanoparticulate Fe 3 O 4 and gambogic acid on phosphatidylinositol 3-kinase/Akt/Bad pathway of LOVO cells

doi: 10.2147/IJN.S32475

Figure Lengend Snippet: Apoptosis ratio of LOVO cells treated with gambogic acid, Fe 3 O 4 magnetic nanoparticles, or magnetic nanoparticles containing Fe 3 O 4 and gambogic acid for 48 hours. ( A ) Controls, ( B ) 60 μg/mL magnetic nanoparticles containing Fe 3 O 4 , ( C ) 0.35 μmol/L gambogic acid, and ( D ) magnetic nanoparticles containing Fe 3 O 4 and gambogic acid (0.35 μmol/L gambogic acid and 60 μg/mL magnetic nanoparticles containing Fe 3 O 4 ).

Article Snippet: A human colorectal cancer cell line (LOVO) purchased from Nanjing Keygen Biotech Co, Ltd was cultured in RPMI 1640 medium containing 10% (v/v) heat-inactivated new-born calf serum, penicillin G 100 U/mL, and streptomycin 100 μg/mL at 37°C in a humidified environment containing 5% CO 2 .

Techniques:

Journal: International Journal of Nanomedicine

Article Title: Synergistic effect of a combination of nanoparticulate Fe 3 O 4 and gambogic acid on phosphatidylinositol 3-kinase/Akt/Bad pathway of LOVO cells

doi: 10.2147/IJN.S32475

Figure Lengend Snippet: Morphological features of LOVO cells after treatment for 48 hours by optical microscope (×400, Wright staining). ( A ) Controls, ( B ) 60 μg/mL magnetic nanoparticles containing Fe 3 O 4 , ( C ) 0.35 μmol/L gambogic acid, and ( D ) magnetic nanoparticles containing Fe 3 O 4 and gambogic acid (0.35 μmol/L gambogic acid with 60 μg/mL magnetic nanoparticles containing Fe 3 O 4 ).

Article Snippet: A human colorectal cancer cell line (LOVO) purchased from Nanjing Keygen Biotech Co, Ltd was cultured in RPMI 1640 medium containing 10% (v/v) heat-inactivated new-born calf serum, penicillin G 100 U/mL, and streptomycin 100 μg/mL at 37°C in a humidified environment containing 5% CO 2 .

Techniques: Microscopy, Wright Stain

Journal: International Journal of Nanomedicine

Article Title: Synergistic effect of a combination of nanoparticulate Fe 3 O 4 and gambogic acid on phosphatidylinositol 3-kinase/Akt/Bad pathway of LOVO cells

doi: 10.2147/IJN.S32475

Figure Lengend Snippet: Nucleolus morphological changes of LOVO cells after treatment for 48 hours under fluorescence microscope (×400, DAPI staining). ( A ) Controls, ( B ) 60 μg/mL magnetic nanoparticles containing Fe 3 O 4 , ( C ) 0.35 μmol/L gambogic acid, and ( D ) magnetic nanoparticles containing Fe 3 O 4 and gambogic acid (0.35 μmol/L gambogic acid with 60 μg/mL magnetic nanoparticles containing Fe 3 O 4 ).

Article Snippet: A human colorectal cancer cell line (LOVO) purchased from Nanjing Keygen Biotech Co, Ltd was cultured in RPMI 1640 medium containing 10% (v/v) heat-inactivated new-born calf serum, penicillin G 100 U/mL, and streptomycin 100 μg/mL at 37°C in a humidified environment containing 5% CO 2 .

Techniques: Fluorescence, Microscopy, Staining

Journal: International Journal of Nanomedicine

Article Title: Synergistic effect of a combination of nanoparticulate Fe 3 O 4 and gambogic acid on phosphatidylinositol 3-kinase/Akt/Bad pathway of LOVO cells

doi: 10.2147/IJN.S32475

Figure Lengend Snippet: Transcription of PI3K, Akt, Bad, cytochrome C, caspase 9, and caspase 3 gene in LOVO cells after treatment comprising GA with or without MNPs-Fe 3 O 4 for 48 hours. Notes: Lane 1, controls; Lane 2, 60 μg/mL MNPs-Fe 3 O 4 ; Lane 3, 0.35 μmol/L GA; Lane 4, GA-MNPs-Fe 3 O 4 (0.35 μmol/L GA with 60 μg/mL MNPs-Fe 3 O 4 ). * P < 0.05; ** P < 0.01, when compared with control group; # P < 0.05, when compared with the GA group. Abbreviations: MNPs-Fe 3 O 4 , magnetic nanoparticles containing Fe 3 O 4 ; GA, gambogic acid.

Article Snippet: A human colorectal cancer cell line (LOVO) purchased from Nanjing Keygen Biotech Co, Ltd was cultured in RPMI 1640 medium containing 10% (v/v) heat-inactivated new-born calf serum, penicillin G 100 U/mL, and streptomycin 100 μg/mL at 37°C in a humidified environment containing 5% CO 2 .

Techniques: Control

Journal: International Journal of Nanomedicine

Article Title: Synergistic effect of a combination of nanoparticulate Fe 3 O 4 and gambogic acid on phosphatidylinositol 3-kinase/Akt/Bad pathway of LOVO cells

doi: 10.2147/IJN.S32475

Figure Lengend Snippet: Expression of PI3K, p-PI3K, Akt, p-Akt, Bad, p-Bad, cytochrome c, pro-caspase 9, caspase 9, and pro-caspase 3, caspase 3 proteins in LOVO cells after treatment comprising GA with or without MNPs-Fe 3 O 4 for 48 hours. Notes: Lane 1, control; lane 2, 60 μg/mL MNPs-Fe 3 O 4 ; lane 3, 0.35 μmol/L GA; lane 4, GA-MNPs-Fe 3 O 4 (0.35 μmol/L GA with 60 μg/mL MNPs-Fe 3 O 4 ). * P < 0.05, ** P < 0.01 compared with the control group; # P < 0.05, when compared with the GA group. Abbreviations: MNPs-Fe 3 O 4 , magnetic nanoparticles containing Fe 3 O 4 ; GA, gambogic acid.

Article Snippet: A human colorectal cancer cell line (LOVO) purchased from Nanjing Keygen Biotech Co, Ltd was cultured in RPMI 1640 medium containing 10% (v/v) heat-inactivated new-born calf serum, penicillin G 100 U/mL, and streptomycin 100 μg/mL at 37°C in a humidified environment containing 5% CO 2 .

Techniques: Expressing, Control

Journal: International Journal of Nanomedicine

Article Title: Synergistic effect of a combination of nanoparticulate Fe 3 O 4 and gambogic acid on phosphatidylinositol 3-kinase/Akt/Bad pathway of LOVO cells

doi: 10.2147/IJN.S32475

Figure Lengend Snippet: The mechanism of GA/GA-MNPs-Fe 3 O 4 on apoptosis of LOVO cells. Notes: →, Promote; ⇢, inhibit; ⇥, block. Abbreviations: MNPs-Fe 3 O 4 , magnetic nanoparticles containing Fe 3 O 4 ; GA, gambogic acid.

Article Snippet: A human colorectal cancer cell line (LOVO) purchased from Nanjing Keygen Biotech Co, Ltd was cultured in RPMI 1640 medium containing 10% (v/v) heat-inactivated new-born calf serum, penicillin G 100 U/mL, and streptomycin 100 μg/mL at 37°C in a humidified environment containing 5% CO 2 .

Techniques: Blocking Assay