Journal: bioRxiv

Article Title: Targeting 3-mercaptopyruvate sulfurtransferase induces cancer stem cell death

doi: 10.64898/2026.03.25.714276

Figure Lengend Snippet: (A) Representative immunoblots showing protein expression of H 2 S-related enzymes and associated metabolic proteins in paired control (CTR) and colorectal tumor tissues. Proteins analyzed include CARS2, CBS (61 kDa and 45 kDa isoforms), SQR, CSE, 3-MST, TST, ETHE1 and SOD1; β-actin was used as a loading control. (B) Quantification of relative protein expression in paired control and tumor tissues. Each line represents paired samples from the same individual (N = 6; 3 females, 3 males). (C) Protein expression of the indicated enzymes in normal colon epithelial cells (NCM356D) and colorectal cancer cell lines (LoVo, HCT116 and SW620), determined by densitometric analysis of immunoblots normalized to β-actin. (D) Growth curves of NCM356D, LoVo, HCT116 and SW620 cells showing total cell number over time. (E) Quantification of cell doubling time for the indicated cell lines. (F) Correlation between relative 3-MST expression and cell doubling time across colon cell lines (R² = 0.9736, two-tailed P = 0.0133; N = 5). Data are presented as mean ± s.e.m from ≥5 independent experiments unless otherwise indicated.

Article Snippet: LoVo human colorectal adenocarcinoma cells (DSMZ ACC-350) were purchased from the German Collection of Microorganisms and Cell Cultures (DSMZ), and SW620 human colorectal adenocarcinoma cells were obtained from the National Cancer Institute (NCI).

Techniques: Western Blot, Expressing, Control, Two Tailed Test

Journal: JCI Insight

Article Title: LIN28B induces a differentiation program through CDX2 in colon cancer

doi: 10.1172/jci.insight.140382

Figure Lengend Snippet: ( A ) Left: subcutaneous xenograft experiments with LoVo cells ( n = 6 per cell type) showed a significant increase in tumor weight with CDX2 KD with sh-CDX2 no. 1 (472.38 ± 126.23 mg at euthanization) or sh-CDX2 no. 2 (353.38 ± 112.90 mg at euthanization) as compared with control cells (123.88 ± 48.86 mg at euthanization). Right: the images of tumors. Scale bars: 10 mm. ( B ) Ki-67 staining in the subcutaneous xenograft tumor of LoVo cells with control or CDX2 KD, representative images (upper) and the quantification (lower). Scale bars: 100 μm. n = 3. ( C ) Transwell chamber invasion assay of LoVo cells with control or CDX2 KD, representative images (upper) and the quantification (lower). Scale bars: 100 μm. n = 3. ( D ) Ki-67 staining in the liver metastatic tumor of LoVo cells with control or CDX2 KD, representative images (upper) and the quantification (lower). Scale bars: 100 μm. n = 3. ( E ) Epithelial-mesenchymal transition (EMT) marker expression (qPCR) in LoVo control/CDX2 KD cells ( n = 3). ( F ) Representative IHC of CDX2, E-cadherin, and CK20 in the liver metastatic tumor of LoVo cells with control or CDX2 KD. Scale bars: 100 μm. Data are presented as mean ± SEM. Unpaired, 2-tailed Student’s t test ( D ) and 1-way ANOVA followed by Dunnett’s multiple-comparison test as post hoc analysis ( A , B , C , and E ) were performed. * P < 0.05, ** P < 0.01.

Article Snippet: CDX2 knockdown in Caco-2 cells and LoVo cells was performed using Genecopoeia shRNA system with HSH000553-31-LVRU6MH (shCDX2 no. 1) and HSH000553-33 LVRU6MH (shCDX no. 2) and CSHCTR001-LVRU6MH (sh-control) constructs.

Techniques: Control, Staining, Invasion Assay, Marker, Expressing, Comparison