monocut lambda (New England Biolabs)

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New England Biolabs monocut lambda
Monocut Lambda, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda+monocut/Lambda+DNA-Mono+Cut+Mix/pm32599056-319-24-28
Average 94 stars, based on 52 article reviews

monocut lambda - by Bioz Stars, 2026-10

94/100 stars

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Agarose Gel Electrophoresis:

Article Title: The DNase Activity of Kaposi’s Sarcoma-Associated Herpesvirus SOX Protein Serves an Important Role in Viral Genome Processing during Lytic Replication
Article Snippet: Subsequently, digested plugs were rinsed with 1X TE and 390 treated with phenylmethanesulfonyl fluoride (PMSF) (Sigma-Aldrich) to remove any residual 391 proteinase K. Next, the plugs were washed with pre-digestion buffer (10 mM MgCl2 and 10 mM 392 Tris-HCl (pH 8.0) and digested with 70 units of PmeI (New England Biolabs Inc.) at 37°C 393 overnight, to linearize the KSHV genome. .. The digested gel plugs were rinsed twice with TE, 394 casted into a 0.7% SeaPlaque GTG agarose gel (Lonza Rockland, Inc.) resolved by PFGE, 395 blotted onto Hybond XL membrane (Amersham, Inc.), and hybridized with a 32 P-labeled KSHV 396 TR- specific probe and lambda monocut (molecular weight marker, NEB). .. DNA isolation and 397 processing for SMARD was done by melting the gel slices from the appropriate region in the 398 PFGE gel at 72°C for 20 min and digesting the melted DNA with GELase enzyme (Epicentre 399 Biotechnologies, Madison, Wisconsin, 1 unit per 50 μl of agarose suspension), followed by 400 incubation of GELase-DNA-agarose mixture at 45°C for 6 h. The resulting DNA was stretched 401 on 3-aminopropyltriethoxysilane (Sigma-Aldrich) coated glass slides.

Article Title: The DNase Activity of Kaposi’s Sarcoma-Associated Herpesvirus SOX Protein Serves an Important Role in Viral Genome Processing during Lytic Replication
Article Snippet: Subsequently, the digested plugs were rinsed with 1× TE and treated with PMSF (Sigma-Aldrich) to remove any residual proteinase K. Next, the plugs were washed with predigestion buffer (10 mM MgCl 2 and 10 mM Tris-HCl [pH 8.0]) and digested with 70 units of PmeI (New England Biolabs Inc.) at 37°C overnight to linearize the KSHV genome. .. The digested gel plugs were rinsed twice with TE, cast into a 0.7% SeaPlaque GTG agarose gel (Lonza Rockland, Inc.), resolved by PFGE, blotted onto a Hybond XL membrane (Amersham, Inc.), and hybridized with a 32 P-labeled KSHV TR-specific probe and lambda monocut (molecular weight marker [NEB]). .. DNA isolation and processing for SMARD were done by melting the gel slices from the appropriate region in the PFGE gel at 72°C for 20 min and digesting the melted DNA with GELase enzyme (Epicentre Biotechnologies, Madison, WI; 1 unit per 50 μl of agarose suspension), followed by incubation of the GELase-DNA-agarose mixture at 45°C for 6 h. The resulting DNA was stretched on 3-aminopropyltriethoxysilane (Sigma-Aldrich)-coated glass slides.

Membrane:

Article Title: The DNase Activity of Kaposi’s Sarcoma-Associated Herpesvirus SOX Protein Serves an Important Role in Viral Genome Processing during Lytic Replication
Article Snippet: Subsequently, the digested plugs were rinsed with 1× TE and treated with PMSF (Sigma-Aldrich) to remove any residual proteinase K. Next, the plugs were washed with predigestion buffer (10 mM MgCl 2 and 10 mM Tris-HCl [pH 8.0]) and digested with 70 units of PmeI (New England Biolabs Inc.) at 37°C overnight to linearize the KSHV genome. .. The digested gel plugs were rinsed twice with TE, cast into a 0.7% SeaPlaque GTG agarose gel (Lonza Rockland, Inc.), resolved by PFGE, blotted onto a Hybond XL membrane (Amersham, Inc.), and hybridized with a 32 P-labeled KSHV TR-specific probe and lambda monocut (molecular weight marker [NEB]). .. DNA isolation and processing for SMARD were done by melting the gel slices from the appropriate region in the PFGE gel at 72°C for 20 min and digesting the melted DNA with GELase enzyme (Epicentre Biotechnologies, Madison, WI; 1 unit per 50 μl of agarose suspension), followed by incubation of the GELase-DNA-agarose mixture at 45°C for 6 h. The resulting DNA was stretched on 3-aminopropyltriethoxysilane (Sigma-Aldrich)-coated glass slides.

Molecular Weight:

Article Title: The DNase Activity of Kaposi’s Sarcoma-Associated Herpesvirus SOX Protein Serves an Important Role in Viral Genome Processing during Lytic Replication
Article Snippet: Subsequently, the digested plugs were rinsed with 1× TE and treated with PMSF (Sigma-Aldrich) to remove any residual proteinase K. Next, the plugs were washed with predigestion buffer (10 mM MgCl 2 and 10 mM Tris-HCl [pH 8.0]) and digested with 70 units of PmeI (New England Biolabs Inc.) at 37°C overnight to linearize the KSHV genome. .. The digested gel plugs were rinsed twice with TE, cast into a 0.7% SeaPlaque GTG agarose gel (Lonza Rockland, Inc.), resolved by PFGE, blotted onto a Hybond XL membrane (Amersham, Inc.), and hybridized with a 32 P-labeled KSHV TR-specific probe and lambda monocut (molecular weight marker [NEB]). .. DNA isolation and processing for SMARD were done by melting the gel slices from the appropriate region in the PFGE gel at 72°C for 20 min and digesting the melted DNA with GELase enzyme (Epicentre Biotechnologies, Madison, WI; 1 unit per 50 μl of agarose suspension), followed by incubation of the GELase-DNA-agarose mixture at 45°C for 6 h. The resulting DNA was stretched on 3-aminopropyltriethoxysilane (Sigma-Aldrich)-coated glass slides.

Marker:

Article Title: The DNase Activity of Kaposi’s Sarcoma-Associated Herpesvirus SOX Protein Serves an Important Role in Viral Genome Processing during Lytic Replication
Article Snippet: Subsequently, the digested plugs were rinsed with 1× TE and treated with PMSF (Sigma-Aldrich) to remove any residual proteinase K. Next, the plugs were washed with predigestion buffer (10 mM MgCl 2 and 10 mM Tris-HCl [pH 8.0]) and digested with 70 units of PmeI (New England Biolabs Inc.) at 37°C overnight to linearize the KSHV genome. .. The digested gel plugs were rinsed twice with TE, cast into a 0.7% SeaPlaque GTG agarose gel (Lonza Rockland, Inc.), resolved by PFGE, blotted onto a Hybond XL membrane (Amersham, Inc.), and hybridized with a 32 P-labeled KSHV TR-specific probe and lambda monocut (molecular weight marker [NEB]). .. DNA isolation and processing for SMARD were done by melting the gel slices from the appropriate region in the PFGE gel at 72°C for 20 min and digesting the melted DNA with GELase enzyme (Epicentre Biotechnologies, Madison, WI; 1 unit per 50 μl of agarose suspension), followed by incubation of the GELase-DNA-agarose mixture at 45°C for 6 h. The resulting DNA was stretched on 3-aminopropyltriethoxysilane (Sigma-Aldrich)-coated glass slides.

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