l1210  (ATCC)

Journal: EJHaem

Article Title: Innate Immune Cell Infiltration Induced by Polatuzumab Vedotin Contributes to the Antitumor Effect in Mouse Models

doi: 10.1002/jha2.70219

Figure Lengend Snippet: Pola‐induced infiltration of MΦs and NK cells contributes to the antitumor effect of Pola in the L1210‐hCD79b‐9 syngeneic mouse model. (A) Sensitivity of L1210 and L1210‐hCD79b‐9 cells to Pola at the indicated concentrations (3 replicates). The data represent the mean + SD. (B) Percentage of the area of CD68‐positive staining cells to the total area of tumor cells and (C) percentage of NCR1‐positive cells among tumor cells after 4 mg/kg ctrl IgG or 4 mg/kg Pola administration in the L1210‐hCD79b‐9 syngeneic mouse model ( n = 8). * p < 0.05 by Student's t ‐test. In dot plots, horizontal bars represent median values. (D) L1210‐hCD79b‐9 tumor growth curves from Day 0 to Day 9 after administration of 4 mg/kg ctrl IgG, 4 mg/kg Pola, or 4 mg/kg Pola plus clodronate liposomes plus anti‐asialo GM1 ( n = 6–7). (E) Numbers of mice presenting L1210‐hCD79b‐9 tumor regression on Day 9 following Pola administration. In vivo experiments (B–E) were conducted in DBA/2J mice. (F) Schematic diagram illustrating the relationship between the antitumor effect of Pola and innate immune cells.

Article Snippet: The DB (human) and L1210 (mouse) cell lines were purchased from the American Type Culture Collection.

Techniques: Staining, Liposomes, In Vivo