Journal: Cellular Oncology

Article Title: BCAA catabolism mediates POU2AF1 propionylation to enhance T-ALL development

doi: 10.1007/s13402-026-01201-w

Figure Lengend Snippet: Targeting BCAT1 metabolism significantly impaired T-ALL progression. ( A – D ) Analysis of the proliferation of EL4 ( A ), L1210 ( B ), Jurkat ( C ), and MOLT-4 ( D ) cells treated in vitro with 10 mM gabapentin (n=4). ( E ) Representative flow cytometric analysis of peripheral blood at 3 weeks post-transplantation from T-ALL model mice following treatment with IgG, anti-PD-1 antibody, low-BCAA, or combined low-BCAA + anti-PD-1 antibody. ( F ) Quantification of the peripheral blood data shown in Panel E (n=5). ( G ) Overall survival analysis of treated mice (n=5). ( H ) Schematic model illustrating how BCAT1-driven branched-chain amino acid metabolism promotes T-ALL progression via Kpr-mediated activation of POU2AF1. The data are presented as the means ± SDs. One-way ANOVA with Tukey’s multiple comparison test ( F ), log-rank test ( G ), and two-way ANOVA with Sidak’s multiple comparison test (A–D) were used for comparisons of statistical significance (*, P <0.05; **, P <0.01; and ***, P <0.001)

Article Snippet: These viral particles were subsequently used to infect NOTCH-GFP+ bone marrow leukemia cells, human cell lines Jurkat and MOLT-4 (ATCC), murine cell lines EL4 and L1210 (ATCC), as well as primary T-ALL patient samples, followed by assessment of in vitro cell proliferation.

Techniques: In Vitro, Transplantation Assay, Activation Assay, Comparison

Journal: STAR Protocols

Article Title: Protocol for assessing regulatory elements in murine heart using an AAV9-based massively parallel reporter assay

doi: 10.1016/j.xpro.2025.103782

Figure Lengend Snippet:

Article Snippet: LB agar plates , Teknova , L5002.

Techniques: Virus, Electroporation, Recombinant, Staining, Reverse Transcription, SYBR Green Assay, Gel Extraction, Software, Plasmid Preparation, Microscopy

Journal: Molecules

Article Title: Development of Resistance to Endoplasmic Reticulum Stress-Inducing Agents in Mouse Leukemic L1210 Cells

doi: 10.3390/molecules25112517

Figure Lengend Snippet: Correlation between proteasomal activity and proliferation rate constants of L1210 cell variants. The data used for the correlation analysis are documented in and and represent value ± S D. The regression line (solid line) was characterized by a 0.95 confidence interval (dashed line). r-correlation coefficient, d.f.-degree of freedom.

Article Snippet: The murine lymphocytic leukemia cell line L1210 (ACC-123, S) was obtained from Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Braunschweig, Germany) and is referred to hereafter as S. Drug-resistant variants were prepared from S cells: i. R cells (P-gp-positive MDR cells) via cultivation in cultivation medium with gradually increasing VCR concentration [ ]; ii.

Techniques: Activity Assay