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kn92  (Tocris)


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    Structured Review

    Tocris kn92
    A. Left: Representative REST immunoblots of primary cortical neurons (DIV14) treated with the cell-permeable competitive Ca 2+ -calmodulin antagonist W7 (20 µM). Calnexin was used as a control for equal loading. Right: Mean (± sem with individual experimental points) REST protein expression shown as a ratio between REST and calnexin immunoreactivities. **p=0.0071; unpaired Student’s t -test (n=5 independent neuronal preparations). B. Left: Representative REST immunoblots of DIV 14 primary cortical neurons treated with the phosphatase inhibitor cyclosporin A (CsA; 1 µM). Calnexin was used as a control for equal loading. Right: Mean (± sem with individual experimental points) REST expression shown as a ratio between REST and calnexin immunoreactivities. p=0.36; unpaired Student’s t -test (n=5 independent neuronal preparations). C. Left: Representative REST immunoblots of DIV14 primary cortical neurons treated with vehicle (Ctrl), the cell-permeable CaMK inhibitor KN93 (10 µM), or its inactive derivative <t>KN92</t> (10 µM). Calnexin was used as a control for equal loading. Right: Mean (± sem with individual experimental points) REST protein expression shown as a ratio between REST and calnexin immunoreactivities for the three treatments. *p=0.031, Ctrl vs KN93; *p=0.032, KN93 vs KN92; one-way ANOVA/Holm-Sidak’s tests (n=5 independent neuronal preparations).
    Kn92, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kn92/product/Tocris
    Average 93 stars, based on 1 article reviews
    kn92 - by Bioz Stars, 2026-04
    93/100 stars

    Images

    1) Product Images from "REST/NRSF phosphorylation by CaMKIV regulates its transcriptional repressor activity and half-life"

    Article Title: REST/NRSF phosphorylation by CaMKIV regulates its transcriptional repressor activity and half-life

    Journal: bioRxiv

    doi: 10.1101/2025.09.04.674163

    A. Left: Representative REST immunoblots of primary cortical neurons (DIV14) treated with the cell-permeable competitive Ca 2+ -calmodulin antagonist W7 (20 µM). Calnexin was used as a control for equal loading. Right: Mean (± sem with individual experimental points) REST protein expression shown as a ratio between REST and calnexin immunoreactivities. **p=0.0071; unpaired Student’s t -test (n=5 independent neuronal preparations). B. Left: Representative REST immunoblots of DIV 14 primary cortical neurons treated with the phosphatase inhibitor cyclosporin A (CsA; 1 µM). Calnexin was used as a control for equal loading. Right: Mean (± sem with individual experimental points) REST expression shown as a ratio between REST and calnexin immunoreactivities. p=0.36; unpaired Student’s t -test (n=5 independent neuronal preparations). C. Left: Representative REST immunoblots of DIV14 primary cortical neurons treated with vehicle (Ctrl), the cell-permeable CaMK inhibitor KN93 (10 µM), or its inactive derivative KN92 (10 µM). Calnexin was used as a control for equal loading. Right: Mean (± sem with individual experimental points) REST protein expression shown as a ratio between REST and calnexin immunoreactivities for the three treatments. *p=0.031, Ctrl vs KN93; *p=0.032, KN93 vs KN92; one-way ANOVA/Holm-Sidak’s tests (n=5 independent neuronal preparations).
    Figure Legend Snippet: A. Left: Representative REST immunoblots of primary cortical neurons (DIV14) treated with the cell-permeable competitive Ca 2+ -calmodulin antagonist W7 (20 µM). Calnexin was used as a control for equal loading. Right: Mean (± sem with individual experimental points) REST protein expression shown as a ratio between REST and calnexin immunoreactivities. **p=0.0071; unpaired Student’s t -test (n=5 independent neuronal preparations). B. Left: Representative REST immunoblots of DIV 14 primary cortical neurons treated with the phosphatase inhibitor cyclosporin A (CsA; 1 µM). Calnexin was used as a control for equal loading. Right: Mean (± sem with individual experimental points) REST expression shown as a ratio between REST and calnexin immunoreactivities. p=0.36; unpaired Student’s t -test (n=5 independent neuronal preparations). C. Left: Representative REST immunoblots of DIV14 primary cortical neurons treated with vehicle (Ctrl), the cell-permeable CaMK inhibitor KN93 (10 µM), or its inactive derivative KN92 (10 µM). Calnexin was used as a control for equal loading. Right: Mean (± sem with individual experimental points) REST protein expression shown as a ratio between REST and calnexin immunoreactivities for the three treatments. *p=0.031, Ctrl vs KN93; *p=0.032, KN93 vs KN92; one-way ANOVA/Holm-Sidak’s tests (n=5 independent neuronal preparations).

    Techniques Used: Western Blot, Control, Expressing



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    A. Left: Representative REST immunoblots of primary cortical neurons (DIV14) treated with the cell-permeable competitive Ca 2+ -calmodulin antagonist W7 (20 µM). Calnexin was used as a control for equal loading. Right: Mean (± sem with individual experimental points) REST protein expression shown as a ratio between REST and calnexin immunoreactivities. **p=0.0071; unpaired Student’s t -test (n=5 independent neuronal preparations). B. Left: Representative REST immunoblots of DIV 14 primary cortical neurons treated with the phosphatase inhibitor cyclosporin A (CsA; 1 µM). Calnexin was used as a control for equal loading. Right: Mean (± sem with individual experimental points) REST expression shown as a ratio between REST and calnexin immunoreactivities. p=0.36; unpaired Student’s t -test (n=5 independent neuronal preparations). C. Left: Representative REST immunoblots of DIV14 primary cortical neurons treated with vehicle (Ctrl), the cell-permeable CaMK inhibitor KN93 (10 µM), or its inactive derivative <t>KN92</t> (10 µM). Calnexin was used as a control for equal loading. Right: Mean (± sem with individual experimental points) REST protein expression shown as a ratio between REST and calnexin immunoreactivities for the three treatments. *p=0.031, Ctrl vs KN93; *p=0.032, KN93 vs KN92; one-way ANOVA/Holm-Sidak’s tests (n=5 independent neuronal preparations).
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    A. Left: Representative REST immunoblots of primary cortical neurons (DIV14) treated with the cell-permeable competitive Ca 2+ -calmodulin antagonist W7 (20 µM). Calnexin was used as a control for equal loading. Right: Mean (± sem with individual experimental points) REST protein expression shown as a ratio between REST and calnexin immunoreactivities. **p=0.0071; unpaired Student’s t -test (n=5 independent neuronal preparations). B. Left: Representative REST immunoblots of DIV 14 primary cortical neurons treated with the phosphatase inhibitor cyclosporin A (CsA; 1 µM). Calnexin was used as a control for equal loading. Right: Mean (± sem with individual experimental points) REST expression shown as a ratio between REST and calnexin immunoreactivities. p=0.36; unpaired Student’s t -test (n=5 independent neuronal preparations). C. Left: Representative REST immunoblots of DIV14 primary cortical neurons treated with vehicle (Ctrl), the cell-permeable CaMK inhibitor KN93 (10 µM), or its inactive derivative <t>KN92</t> (10 µM). Calnexin was used as a control for equal loading. Right: Mean (± sem with individual experimental points) REST protein expression shown as a ratio between REST and calnexin immunoreactivities for the three treatments. *p=0.031, Ctrl vs KN93; *p=0.032, KN93 vs KN92; one-way ANOVA/Holm-Sidak’s tests (n=5 independent neuronal preparations).
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    A. Left: Representative REST immunoblots of primary cortical neurons (DIV14) treated with the cell-permeable competitive Ca 2+ -calmodulin antagonist W7 (20 µM). Calnexin was used as a control for equal loading. Right: Mean (± sem with individual experimental points) REST protein expression shown as a ratio between REST and calnexin immunoreactivities. **p=0.0071; unpaired Student’s t -test (n=5 independent neuronal preparations). B. Left: Representative REST immunoblots of DIV 14 primary cortical neurons treated with the phosphatase inhibitor cyclosporin A (CsA; 1 µM). Calnexin was used as a control for equal loading. Right: Mean (± sem with individual experimental points) REST expression shown as a ratio between REST and calnexin immunoreactivities. p=0.36; unpaired Student’s t -test (n=5 independent neuronal preparations). C. Left: Representative REST immunoblots of DIV14 primary cortical neurons treated with vehicle (Ctrl), the cell-permeable CaMK inhibitor KN93 (10 µM), or its inactive derivative <t>KN92</t> (10 µM). Calnexin was used as a control for equal loading. Right: Mean (± sem with individual experimental points) REST protein expression shown as a ratio between REST and calnexin immunoreactivities for the three treatments. *p=0.031, Ctrl vs KN93; *p=0.032, KN93 vs KN92; one-way ANOVA/Holm-Sidak’s tests (n=5 independent neuronal preparations).
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    Image Search Results


    A. Left: Representative REST immunoblots of primary cortical neurons (DIV14) treated with the cell-permeable competitive Ca 2+ -calmodulin antagonist W7 (20 µM). Calnexin was used as a control for equal loading. Right: Mean (± sem with individual experimental points) REST protein expression shown as a ratio between REST and calnexin immunoreactivities. **p=0.0071; unpaired Student’s t -test (n=5 independent neuronal preparations). B. Left: Representative REST immunoblots of DIV 14 primary cortical neurons treated with the phosphatase inhibitor cyclosporin A (CsA; 1 µM). Calnexin was used as a control for equal loading. Right: Mean (± sem with individual experimental points) REST expression shown as a ratio between REST and calnexin immunoreactivities. p=0.36; unpaired Student’s t -test (n=5 independent neuronal preparations). C. Left: Representative REST immunoblots of DIV14 primary cortical neurons treated with vehicle (Ctrl), the cell-permeable CaMK inhibitor KN93 (10 µM), or its inactive derivative KN92 (10 µM). Calnexin was used as a control for equal loading. Right: Mean (± sem with individual experimental points) REST protein expression shown as a ratio between REST and calnexin immunoreactivities for the three treatments. *p=0.031, Ctrl vs KN93; *p=0.032, KN93 vs KN92; one-way ANOVA/Holm-Sidak’s tests (n=5 independent neuronal preparations).

    Journal: bioRxiv

    Article Title: REST/NRSF phosphorylation by CaMKIV regulates its transcriptional repressor activity and half-life

    doi: 10.1101/2025.09.04.674163

    Figure Lengend Snippet: A. Left: Representative REST immunoblots of primary cortical neurons (DIV14) treated with the cell-permeable competitive Ca 2+ -calmodulin antagonist W7 (20 µM). Calnexin was used as a control for equal loading. Right: Mean (± sem with individual experimental points) REST protein expression shown as a ratio between REST and calnexin immunoreactivities. **p=0.0071; unpaired Student’s t -test (n=5 independent neuronal preparations). B. Left: Representative REST immunoblots of DIV 14 primary cortical neurons treated with the phosphatase inhibitor cyclosporin A (CsA; 1 µM). Calnexin was used as a control for equal loading. Right: Mean (± sem with individual experimental points) REST expression shown as a ratio between REST and calnexin immunoreactivities. p=0.36; unpaired Student’s t -test (n=5 independent neuronal preparations). C. Left: Representative REST immunoblots of DIV14 primary cortical neurons treated with vehicle (Ctrl), the cell-permeable CaMK inhibitor KN93 (10 µM), or its inactive derivative KN92 (10 µM). Calnexin was used as a control for equal loading. Right: Mean (± sem with individual experimental points) REST protein expression shown as a ratio between REST and calnexin immunoreactivities for the three treatments. *p=0.031, Ctrl vs KN93; *p=0.032, KN93 vs KN92; one-way ANOVA/Holm-Sidak’s tests (n=5 independent neuronal preparations).

    Article Snippet: W7 (N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide hydrochloride), KN92, KN93, bicuculline, CGP58845, D-APV, and tetrodotoxin were from Tocris (Bristol, UK).

    Techniques: Western Blot, Control, Expressing