Journal: Experimental eye research

Article Title: Signaling pathways activated by resolvin E1 to stimulate mucin secretion and increase intracellular Ca 2+ in cultured rat conjunctival goblet cells

doi: 10.1016/j.exer.2018.04.015

Figure Lengend Snippet: Cultured goblet cells were preincubated with the Ca2+/CaMK inhibitor KN93 and its negative control KN92, both at 10−7 M for 30 min. [Ca2+]i over time is shown in A. Change in peak [Ca2+]i is shown in B. Data are mean ± SEM from 4 independent experiments. * indicates significant difference from basal.

Article Snippet: Ro-318220, {"type":"entrez-nucleotide","attrs":{"text":"U73122","term_id":"4098075","term_text":"U73122"}} U73122 and {"type":"entrez-nucleotide","attrs":{"text":"U73343","term_id":"1688125","term_text":"U73343"}} U73343 , KN92 and KN93 were purchased from Tocris Bioscience (Ellisville, MO).

Techniques: Cell Culture, Negative Control

Journal: Experimental eye research

Article Title: Signaling pathways activated by resolvin E1 to stimulate mucin secretion and increase intracellular Ca 2+ in cultured rat conjunctival goblet cells

doi: 10.1016/j.exer.2018.04.015

Figure Lengend Snippet: Cultured goblet cells were incubated with U73122, U73343, 2-APB, Ro 31–8220, 1-but, t-but, aris acid, KN93, or KN92 before stimulation with RvE1 (10−9 M). Glycoconjugate secretion was measured. Data are mean ± SEM from 4 independent experiments and shown as fold above basal, which set to 1. * indicates significant difference from basal; # indicates significance from RvE1 alone.

Article Snippet: Ro-318220, {"type":"entrez-nucleotide","attrs":{"text":"U73122","term_id":"4098075","term_text":"U73122"}} U73122 and {"type":"entrez-nucleotide","attrs":{"text":"U73343","term_id":"1688125","term_text":"U73343"}} U73343 , KN92 and KN93 were purchased from Tocris Bioscience (Ellisville, MO).

Techniques: Cell Culture, Incubation

Journal: Advanced Science

Article Title: Decreased RYR2 Cluster Size and Abnormal SR Ca 2+ Release Contribute to Arrhythmogenesis in TMEM43 ‐Related ARVC

doi: 10.1002/advs.202512058

Figure Lengend Snippet: Decreased RYR2 cluster size leads to abnormal SR Ca 2+ release that is causally linked to the arrhythmic phenotypes in ARVC iPSC‐CMs. A,B) Representative Ca 2+ transient tracings from GC and ARVC iPSC‐CMs. Red arrows indicate the irregular arrhythmia‐like Ca 2+ transients. C–G) Bar graphs to compare key Ca 2+ transient parameters between GC and ARVC iPSC‐CMs. n = 79–95 cells in 2 different iPSC lines. H) Representative traces of cytosolic Ca 2+ fluorescence in control (CON), ARVC, and GC iPSC‐CMs in normal Tyrode's solution and exposed to 0 Na + , 0 Ca 2+ solution containing 1 m m tetracaine and 10 m m caffeine. I–L) Bar graphs to compare the Ca 2+ transient amplitude, SR Ca 2+ leak, SR Ca 2+ load, and fractional release between control, ARVC, and GC iPSC‐CMs. n = 87–148 cells in 2 different iPSC lines. M) Bar graph to compare the percentage of cells with irregular Ca 2+ transients between ARVC iPSC‐CMs treated with DMSO (vehicle), KN92, and KN93. n = 98–177 cells in 2 different iPSC lines. N) Bar graph to compare the percentage of cells with arrhythmias between ARVC iPSC‐CMs treated with DMSO, KN92, and KN93. n = 32–37 cells in 2 different iPSC lines. O) Representative Tau‐STED imaging of RYR2 (red) from control, ARVC, and GC iPSC‐CMs. P) Representative threshold binary images of (O). Q) Representative images showing RYR2 clusters in (P) were identified and labeled. R) Bar graph to compare the mean cluster size between control, ARVC, and GC iPSC‐CMs. n = 1973–5343 clusters. Data were collected from 2 different iPSC lines. S) Bar graph to compare the mean cluster density between control, ARVC, and GC iPSC‐CMs. n = 10–23 cells in 2 different iPSC lines. T) The distribution of the number of RYR2 s from clusters over a certain size across increasing cluster size. The vertical dashed line indicates the cluster size of 50. Scale bar, 500 nm. For all panels, data are represented as mean ± SEM. * p < 0.05; *** p < 0.001; **** p < 0.0001, unpaired two‐tailed Student's t ‐test (C–G) or one‐way ANOVA followed by Tukey's HSD post‐hoc test (I–L, R, S).

Article Snippet: KN92 and KN93 were purchased from Selleck (S6507) and Abcam ( Ab120980 ), respectively, and stock solutions were both prepared in 1 m m DMSO.

Techniques: Fluorescence, Control, Imaging, Labeling, Two Tailed Test