Activity Assay:Article Title: From Yufeining to Kaempferol: Multi-Target Anti-Inflammatory, Antioxidant, and Anti-Apoptotic Mechanisms Targeting the STAT3–TP53–IL1B Signaling Network in COPD Therapy
Article Snippet: .. Kaempferol was supplied by MCE Inc. (Shanghai, China), H-DMEM medium was purchased from Gibco Inc. (Carlsbad, CA, USA), fetal bovine serum was provided by Clark Bioscience Inc. (Hampton, VA, USA), trypsin was obtained from Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China), Trizol reagent was purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA), chloroform, isopropanol and absolute ethanol were all supplied by Beijing Chemical Works (Beijing, China), dimethyl sulfoxide (DMSO) was acquired from Xilong Chemical Co., Ltd. (Guangzhou, China), RNase-free water was provided by Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China), Annexin V-FITC apoptosis detection kit and total SOD activity assay kit were both obtained from Beyotime Biotechnology Co., Ltd. (Shanghai, China), micro malondialdehyde assay kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China), Zunyi cigarette was supplied by Zunyi Cigarette Factory, China Tobacco Guizhou Industrial Co., Ltd. (Zunyi, China), dexamethasone acetate tablets were provided by Zhejiang Xianju Pharmaceutical Co., Ltd. (Taizhou, China), 4% paraformaldehyde solution was purchased from Shanghai Shangbao Biotechnology Co., Ltd. (Shanghai, China), mouse tumor necrosis factor-α (TNF-α) ELISA kit, mouse interleukin-1β (IL-1β) ELISA reagent and mouse interleukin-6 (IL-6) ELISA kit were all acquired from Hubei Pumei Biotechnology Co., Ltd. (Wuhan, China), H&E staining solution and Masson staining solution were both supplied by Wuhan Sevier Biotechnology Co., Ltd. (Wuhan, China), sterile PBS was provided by Euroimmun (Beijing, China), and RNAeasyTM Animal RNA Extraction Kit (Spin Column) was purchased from Beyotime Biotechnology Co., Ltd. .. A total of 60 SPF-grade female Balb/c mice weighing 18 to 22 g were obtained from 536 the Changchun Yisi Experimental Animal Technology Co., Ltd. (Changchun, China).
Enzyme-linked Immunosorbent Assay:Article Title: From Yufeining to Kaempferol: Multi-Target Anti-Inflammatory, Antioxidant, and Anti-Apoptotic Mechanisms Targeting the STAT3–TP53–IL1B Signaling Network in COPD Therapy
Article Snippet: .. Kaempferol was supplied by MCE Inc. (Shanghai, China), H-DMEM medium was purchased from Gibco Inc. (Carlsbad, CA, USA), fetal bovine serum was provided by Clark Bioscience Inc. (Hampton, VA, USA), trypsin was obtained from Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China), Trizol reagent was purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA), chloroform, isopropanol and absolute ethanol were all supplied by Beijing Chemical Works (Beijing, China), dimethyl sulfoxide (DMSO) was acquired from Xilong Chemical Co., Ltd. (Guangzhou, China), RNase-free water was provided by Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China), Annexin V-FITC apoptosis detection kit and total SOD activity assay kit were both obtained from Beyotime Biotechnology Co., Ltd. (Shanghai, China), micro malondialdehyde assay kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China), Zunyi cigarette was supplied by Zunyi Cigarette Factory, China Tobacco Guizhou Industrial Co., Ltd. (Zunyi, China), dexamethasone acetate tablets were provided by Zhejiang Xianju Pharmaceutical Co., Ltd. (Taizhou, China), 4% paraformaldehyde solution was purchased from Shanghai Shangbao Biotechnology Co., Ltd. (Shanghai, China), mouse tumor necrosis factor-α (TNF-α) ELISA kit, mouse interleukin-1β (IL-1β) ELISA reagent and mouse interleukin-6 (IL-6) ELISA kit were all acquired from Hubei Pumei Biotechnology Co., Ltd. (Wuhan, China), H&E staining solution and Masson staining solution were both supplied by Wuhan Sevier Biotechnology Co., Ltd. (Wuhan, China), sterile PBS was provided by Euroimmun (Beijing, China), and RNAeasyTM Animal RNA Extraction Kit (Spin Column) was purchased from Beyotime Biotechnology Co., Ltd. .. A total of 60 SPF-grade female Balb/c mice weighing 18 to 22 g were obtained from 536 the Changchun Yisi Experimental Animal Technology Co., Ltd. (Changchun, China).
Staining:Article Title: From Yufeining to Kaempferol: Multi-Target Anti-Inflammatory, Antioxidant, and Anti-Apoptotic Mechanisms Targeting the STAT3–TP53–IL1B Signaling Network in COPD Therapy
Article Snippet: .. Kaempferol was supplied by MCE Inc. (Shanghai, China), H-DMEM medium was purchased from Gibco Inc. (Carlsbad, CA, USA), fetal bovine serum was provided by Clark Bioscience Inc. (Hampton, VA, USA), trypsin was obtained from Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China), Trizol reagent was purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA), chloroform, isopropanol and absolute ethanol were all supplied by Beijing Chemical Works (Beijing, China), dimethyl sulfoxide (DMSO) was acquired from Xilong Chemical Co., Ltd. (Guangzhou, China), RNase-free water was provided by Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China), Annexin V-FITC apoptosis detection kit and total SOD activity assay kit were both obtained from Beyotime Biotechnology Co., Ltd. (Shanghai, China), micro malondialdehyde assay kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China), Zunyi cigarette was supplied by Zunyi Cigarette Factory, China Tobacco Guizhou Industrial Co., Ltd. (Zunyi, China), dexamethasone acetate tablets were provided by Zhejiang Xianju Pharmaceutical Co., Ltd. (Taizhou, China), 4% paraformaldehyde solution was purchased from Shanghai Shangbao Biotechnology Co., Ltd. (Shanghai, China), mouse tumor necrosis factor-α (TNF-α) ELISA kit, mouse interleukin-1β (IL-1β) ELISA reagent and mouse interleukin-6 (IL-6) ELISA kit were all acquired from Hubei Pumei Biotechnology Co., Ltd. (Wuhan, China), H&E staining solution and Masson staining solution were both supplied by Wuhan Sevier Biotechnology Co., Ltd. (Wuhan, China), sterile PBS was provided by Euroimmun (Beijing, China), and RNAeasyTM Animal RNA Extraction Kit (Spin Column) was purchased from Beyotime Biotechnology Co., Ltd. .. A total of 60 SPF-grade female Balb/c mice weighing 18 to 22 g were obtained from 536 the Changchun Yisi Experimental Animal Technology Co., Ltd. (Changchun, China).
Sterility:Article Title: From Yufeining to Kaempferol: Multi-Target Anti-Inflammatory, Antioxidant, and Anti-Apoptotic Mechanisms Targeting the STAT3–TP53–IL1B Signaling Network in COPD Therapy
Article Snippet: .. Kaempferol was supplied by MCE Inc. (Shanghai, China), H-DMEM medium was purchased from Gibco Inc. (Carlsbad, CA, USA), fetal bovine serum was provided by Clark Bioscience Inc. (Hampton, VA, USA), trypsin was obtained from Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China), Trizol reagent was purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA), chloroform, isopropanol and absolute ethanol were all supplied by Beijing Chemical Works (Beijing, China), dimethyl sulfoxide (DMSO) was acquired from Xilong Chemical Co., Ltd. (Guangzhou, China), RNase-free water was provided by Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China), Annexin V-FITC apoptosis detection kit and total SOD activity assay kit were both obtained from Beyotime Biotechnology Co., Ltd. (Shanghai, China), micro malondialdehyde assay kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China), Zunyi cigarette was supplied by Zunyi Cigarette Factory, China Tobacco Guizhou Industrial Co., Ltd. (Zunyi, China), dexamethasone acetate tablets were provided by Zhejiang Xianju Pharmaceutical Co., Ltd. (Taizhou, China), 4% paraformaldehyde solution was purchased from Shanghai Shangbao Biotechnology Co., Ltd. (Shanghai, China), mouse tumor necrosis factor-α (TNF-α) ELISA kit, mouse interleukin-1β (IL-1β) ELISA reagent and mouse interleukin-6 (IL-6) ELISA kit were all acquired from Hubei Pumei Biotechnology Co., Ltd. (Wuhan, China), H&E staining solution and Masson staining solution were both supplied by Wuhan Sevier Biotechnology Co., Ltd. (Wuhan, China), sterile PBS was provided by Euroimmun (Beijing, China), and RNAeasyTM Animal RNA Extraction Kit (Spin Column) was purchased from Beyotime Biotechnology Co., Ltd. .. A total of 60 SPF-grade female Balb/c mice weighing 18 to 22 g were obtained from 536 the Changchun Yisi Experimental Animal Technology Co., Ltd. (Changchun, China).
RNA Extraction:Article Title: From Yufeining to Kaempferol: Multi-Target Anti-Inflammatory, Antioxidant, and Anti-Apoptotic Mechanisms Targeting the STAT3–TP53–IL1B Signaling Network in COPD Therapy
Article Snippet: .. Kaempferol was supplied by MCE Inc. (Shanghai, China), H-DMEM medium was purchased from Gibco Inc. (Carlsbad, CA, USA), fetal bovine serum was provided by Clark Bioscience Inc. (Hampton, VA, USA), trypsin was obtained from Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China), Trizol reagent was purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA), chloroform, isopropanol and absolute ethanol were all supplied by Beijing Chemical Works (Beijing, China), dimethyl sulfoxide (DMSO) was acquired from Xilong Chemical Co., Ltd. (Guangzhou, China), RNase-free water was provided by Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China), Annexin V-FITC apoptosis detection kit and total SOD activity assay kit were both obtained from Beyotime Biotechnology Co., Ltd. (Shanghai, China), micro malondialdehyde assay kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China), Zunyi cigarette was supplied by Zunyi Cigarette Factory, China Tobacco Guizhou Industrial Co., Ltd. (Zunyi, China), dexamethasone acetate tablets were provided by Zhejiang Xianju Pharmaceutical Co., Ltd. (Taizhou, China), 4% paraformaldehyde solution was purchased from Shanghai Shangbao Biotechnology Co., Ltd. (Shanghai, China), mouse tumor necrosis factor-α (TNF-α) ELISA kit, mouse interleukin-1β (IL-1β) ELISA reagent and mouse interleukin-6 (IL-6) ELISA kit were all acquired from Hubei Pumei Biotechnology Co., Ltd. (Wuhan, China), H&E staining solution and Masson staining solution were both supplied by Wuhan Sevier Biotechnology Co., Ltd. (Wuhan, China), sterile PBS was provided by Euroimmun (Beijing, China), and RNAeasyTM Animal RNA Extraction Kit (Spin Column) was purchased from Beyotime Biotechnology Co., Ltd. .. A total of 60 SPF-grade female Balb/c mice weighing 18 to 22 g were obtained from 536 the Changchun Yisi Experimental Animal Technology Co., Ltd. (Changchun, China).
In Vitro:
other:
Saline:Article Title: Kaempferol Protects Against Hindlimb Ischemia-Reperfusion Injury via Activation of PPARα Signaling.
Article Snippet: Limb ischemia‐reperfusion (I/R) injury is a life‐threatening complication of acute limb ischemia that can result in severe skeletal muscle damage and limb loss, yet effective pharmacological therapies remain limited.. Kaempferol (KAE), a naturally occurring dietary flavonoid with well‐documented anti‐inflammatory, antioxidant, and anti‐apoptotic properties, has been shown to confer protection in myocardial and cerebral I/R models.. Nevertheless, its therapeutic potential and molecular mechanisms in limb I/R injury have not yet been elucidated.
Formulation:Article Title: Kaempferol Protects Against Hindlimb Ischemia-Reperfusion Injury via Activation of PPARα Signaling.
Article Snippet: Limb ischemia‐reperfusion (I/R) injury is a life‐threatening complication of acute limb ischemia that can result in severe skeletal muscle damage and limb loss, yet effective pharmacological therapies remain limited.. Kaempferol (KAE), a naturally occurring dietary flavonoid with well‐documented anti‐inflammatory, antioxidant, and anti‐apoptotic properties, has been shown to confer protection in myocardial and cerebral I/R models.. Nevertheless, its therapeutic potential and molecular mechanisms in limb I/R injury have not yet been elucidated.
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