Journal: Cancer Reports

Article Title: Unraveling the Anti‐Tumor Effects and Molecular Mechanisms of Hairyvein Agrimonia Herb in Gastric Cancer Through Network Pharmacology and Experimental Validation

doi: 10.1002/cnr2.70169

Figure Lengend Snippet: 3D docking patterns of key targets and specific active compounds. (A) Kaempferol binding to JUN crystal structure. (B) Luteolin binding to JUN crystal structure. (C) Quercetin binding to JUN crystal structure. (D) Quercetin binding to HIF1A crystal structure. (E) Luteolin binding to PTGS2 crystal structure. (F) Kaempferol binding to PTGS2 crystal structure. (G) Quercetin binding to PTGS2 crystal structure. (H) (+)‐Catechin binding to PTGS2 crystal structure.

Article Snippet: Luteolin, quercetin, kaempferol, and (+)‐catechin were obtained from the MCE (Shanghai, China).

Techniques: Binding Assay

Journal: Cancer Reports

Article Title: Unraveling the Anti‐Tumor Effects and Molecular Mechanisms of Hairyvein Agrimonia Herb in Gastric Cancer Through Network Pharmacology and Experimental Validation

doi: 10.1002/cnr2.70169

Figure Lengend Snippet: In vitro experiments validating the inhibition of gastric cancer cells by Hairyvein Agrimonia (A) Proliferation rates of Control and luteolin, quercetin, kaempferol, and (+)‐catechin, AGS, and HGC‐27 cells treated as indicated were measured by MTT assay. (B) Flow cytometry analyzed each group cell's cell cycle distribution and treated as indicated. (C, D) After 10 days, cells were stained with crystal violet and imaged to assess their colony formation ability and invasion ability. (E) Immunohistochemistry data from public protein databases show high JUN, HIF1A, and PTGS2 expression in gastric cancer tissues. (G) The PCR results of PTGS2 in AGS and HGC‐27 cells were treated as indicated. (F, H) The western blot results of PTGS2 in AGS and HGC‐27 cells were treated as indicated.

Article Snippet: Luteolin, quercetin, kaempferol, and (+)‐catechin were obtained from the MCE (Shanghai, China).

Techniques: In Vitro, Inhibition, Control, MTT Assay, Flow Cytometry, Staining, Immunohistochemistry, Expressing, Western Blot

Journal: Molecules

Article Title: Divergent and Overlapping Roles for Selected Phytochemicals in the Regulation of Pathological Cardiac Hypertrophy

doi: 10.3390/molecules26051210

Figure Lengend Snippet: Dietary inhibitors of Cardiac hypertrophy.

Article Snippet: The following dietary compounds were purchased from Selleckchem: apigenin (Selleckchem, Cat#- S2262), baicalein (Selleckchem, Cat#- S2269), baicalin (Selleckchem, Cat#- S2268), berberine hydrochloride (Selleckchem, Cat#- S2271), Cat#- S2268), caffeic acid (Selleckchem, Cat#- S2277), dihydromyricetin (Selleckchem, Cat#- S2399), emodin (Selleckchem, Cat#- S2295), (−)-epigallacatechin gallate (Selleckchem, Cat#- 2250), gossypol acetate (Selleckchem, Cat#- S2303), hematoxylin (Selleckchem, Cat#- S2384), indirubin (Selleckchem, Cat#- S2386), kaempferol (Selleckchem, Cat#- S2314), luteolin (Selleckchem, Cat#- S2320), morin hydrate (Selleckchem, Cat#- S2325), myricetin (Selleckchem, Cat#- S2326), myricitrin (Selleckchem, Cat#- S2327), palmatine chloride (Selleckchem, Cat#- S2397) and quercetin dihydrate (Selleckchem, Cat#- S2347).

Techniques: Inhibition

Journal: Aging

Article Title: Fisetin inhibits the proliferation, migration and invasion of pancreatic cancer by targeting PI3K/AKT/mTOR signaling.

doi: 10.18632/aging.203713

Figure Lengend Snippet: Figure 1. Fisetin inhibits the proliferation of PANC-1 cells. (A) PANC-1 cells were inoculated with Astragaline, Afzelin, Quercetin 3-O-α- L-arabinoside, Narcissoside, Avicularin, Fisetin, Herbacetin, 3'-Hydroxyflavanone and Kaempferol-3-O-rutinoside (50 μM), and the cell index was recorded by RTCA. (B) Histogram exhibiting the repression rate of different compounds. (C) Colony formation assessment of PANC-1 cells inoculated with 0,50 and 100 μM fisetin. (D) Histogram illustrating the number of colonies in each group. (E) Ki67 Immunofluorescence staining of PANC-1 cells inoculated with 0,50 and 100 μM fisetin for 24h. (F) Histogram illustrating the Ki67 positive rate of PANC-1 cells in each group. All assays were replicated thrice, and data are given as means±SD.*p<.05, **p<.01,***p<.001, in contrast with the controls.

Article Snippet: Astragaline (CAS No. : 480-10-4, purity of 99.85%), Afzelin (CAS No. : 482-39-3, purity of 99.62%), Quercetin 3-O-α-L-arabinoside (CAS No. : 22688-79-5, purity of 99.83%), Narcissoside (CAS No. : 604-80-8), Avicularin (CAS No. : 572-30-5), Fisetin (CAS No. : 528-48-3, purity of 98.02%), Herbacetin (CAS No. : 527-95-7, purity > 99.0%) were purchased from MedChemExpress (MCE, Shanghai, China).

Techniques: Immunofluorescence, Staining

Journal: Scientific Reports

Article Title: Comprehensive characterization of flavonoid derivatives in young leaves of core-collected soybean ( Glycine max L.) cultivars based on high-resolution mass spectrometry

doi: 10.1038/s41598-022-18226-4

Figure Lengend Snippet: Characterization of eighty-three flavonoid derivatives from young leaves of 21 soybean cultivars using UPLC-DAD-QToF/MS.

Article Snippet: Reference standards of apigenin, daidzein, daidzin, formononetin, genistein and genistin were obtained from Sigma-Aldrich Co. (St Louis, MO, USA); astragalin, calendoflavoside, cosmosiin, cynaroside, glycitin, hyperoside, isoquercitrin, isorhamnetin 3- O -glucoside, luteolin, narcissin, nicotiflorin and rutin as well as 6-methoxyluteolin and 6-methoxyflavone as internal standards were purchased from Extrasynthese (Genay, France); 6- O -malonyldaidzin and 6- O -malonylgenistin from Synthose Inc. (Ontario, Canada); kaempferol 3- O -gentiobioside, quercetin 3- O -gentiobioside and quercetin 3- O -sophoroside from PhytoLab GmbH & Co. (Vestenbergsgreuth, Germany); Cacticin and trifolin from MedChemExpress (Monmouth Junction, USA).

Techniques:

Journal: ACS Omega

Article Title: Extraction of Functional Components from Freeze-Dried Angelica furcijuga Leaves Using Supercritical Carbon Dioxide

doi: 10.1021/acsomega.1c06105

Figure Lengend Snippet: Extraction yields of functional components as a function of extraction time: (a) kaempferol, (b) ferulic acid, (c) ligustilide, and (d–e) butylidenephthalide.

Article Snippet: Kaempferol (K0117, LKT Labs, Inc.), ferulic acid (F1669, LKT Labs, Inc.), ligustilide (L397900, Toronto Research Chemicals Inc.), Z -butylidenephthalide (W333301, Sigma-Aldrich Japan G.K.), and E -butylidenephthalide (W333301, Sigma-Aldrich Japan G.K.) were used as chemical reference standards for chromatographic analysis.

Techniques: Extraction, Functional Assay

Journal: ACS Omega

Article Title: Extraction of Functional Components from Freeze-Dried Angelica furcijuga Leaves Using Supercritical Carbon Dioxide

doi: 10.1021/acsomega.1c06105

Figure Lengend Snippet: Total extraction yields of functional components at various extraction conditions: (a) kaempferol, (b) ferulic acid, (c) ligustilide, and (d–e) butylidenephthalide.

Article Snippet: Kaempferol (K0117, LKT Labs, Inc.), ferulic acid (F1669, LKT Labs, Inc.), ligustilide (L397900, Toronto Research Chemicals Inc.), Z -butylidenephthalide (W333301, Sigma-Aldrich Japan G.K.), and E -butylidenephthalide (W333301, Sigma-Aldrich Japan G.K.) were used as chemical reference standards for chromatographic analysis.

Techniques: Extraction, Functional Assay

Journal: ACS Omega

Article Title: Extraction of Functional Components from Freeze-Dried Angelica furcijuga Leaves Using Supercritical Carbon Dioxide

doi: 10.1021/acsomega.1c06105

Figure Lengend Snippet: Some selected functional components of A. furcijuga : (a) kaempferol, (b) ferulic acid, (c) ligustilide, and (d) butylidenephthalide.

Article Snippet: Kaempferol (K0117, LKT Labs, Inc.), ferulic acid (F1669, LKT Labs, Inc.), ligustilide (L397900, Toronto Research Chemicals Inc.), Z -butylidenephthalide (W333301, Sigma-Aldrich Japan G.K.), and E -butylidenephthalide (W333301, Sigma-Aldrich Japan G.K.) were used as chemical reference standards for chromatographic analysis.

Techniques: Functional Assay

Journal: ACS Omega

Article Title: Extraction of Functional Components from Freeze-Dried Angelica furcijuga Leaves Using Supercritical Carbon Dioxide

doi: 10.1021/acsomega.1c06105

Figure Lengend Snippet: Hansen Solubility Parameters for Selected Functional Components and Pure Solvents a

Article Snippet: Kaempferol (K0117, LKT Labs, Inc.), ferulic acid (F1669, LKT Labs, Inc.), ligustilide (L397900, Toronto Research Chemicals Inc.), Z -butylidenephthalide (W333301, Sigma-Aldrich Japan G.K.), and E -butylidenephthalide (W333301, Sigma-Aldrich Japan G.K.) were used as chemical reference standards for chromatographic analysis.

Techniques: Solubility, Functional Assay

Journal: ACS Omega

Article Title: Extraction of Functional Components from Freeze-Dried Angelica furcijuga Leaves Using Supercritical Carbon Dioxide

doi: 10.1021/acsomega.1c06105

Figure Lengend Snippet: Analysis of Variance (ANOVA) for Experimental Parameters

Article Snippet: Kaempferol (K0117, LKT Labs, Inc.), ferulic acid (F1669, LKT Labs, Inc.), ligustilide (L397900, Toronto Research Chemicals Inc.), Z -butylidenephthalide (W333301, Sigma-Aldrich Japan G.K.), and E -butylidenephthalide (W333301, Sigma-Aldrich Japan G.K.) were used as chemical reference standards for chromatographic analysis.

Techniques: Functional Assay, Extraction

Journal: BMC Cancer

Article Title: RPS6KA1 is a histone acetylation-related oncoprotein in acute myeloid leukemia which is targeted by afzelin

doi: 10.1186/s12885-024-12886-3

Figure Lengend Snippet: Afzelin exerts anti-AML effects by acting on the MAPK pathway and RPS6KA1. A . Chemical structure of afzelin. B . HL-60 and THP-1 cells were treated with different concentrations of afzelin (0, 10, 20, 30, 40 and 50 µM) for 24 h, and cell viability was detected by the CCK-8 assay. C-H . The migration (C), invasion (D), cell cycle (E&F), and apoptosis (G&H) of HL-60 and THP-1 cells after treatment with 30 µM afzelin for 24 h were detected by Transwell assay and flow cytometry, respectively. I&J . Protein levels of p-RPS6KA1, p-ERK1/2, p-JNK, p-p38, and p-MCL-1 in HL-60 and THP-1 cells treated with 30 µM afzelin for 24 h were detected by Western blot. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: Additionally, HL-60 and THP-1 cells were treated with afzelin (purity: 99.62%, CAS No. HY-N1441; MedChemExpress, Shanghai, China) at varying concentrations (0, 10, 20, 30, 40, and 50 μM) for 24 h. Untreated cells served as controls.

Techniques: CCK-8 Assay, Migration, Transwell Assay, Flow Cytometry, Western Blot