il1ra (Proteintech)
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Proteintech
il1ra
Il1ra, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il1ra/IL-1RA+Antibody/pmc12643838-86-13-14
Average 93 stars, based on 15 article reviews
Il1ra, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il1ra/IL-1RA+Antibody/pmc12643838-86-13-14
Average 93 stars, based on 15 article reviews
il1ra - by Bioz Stars,
2026-10
93/100 stars
Images
1) Product Images from "IL1RA + Myeloid-derived Suppressor Cells Activate Epithelial-mesenchymal Transition to Facilitate Lymphatic and Hepatic Metastasis in Pancreatic Ductal Carcinoma"
Article Title: IL1RA + Myeloid-derived Suppressor Cells Activate Epithelial-mesenchymal Transition to Facilitate Lymphatic and Hepatic Metastasis in Pancreatic Ductal Carcinoma
Journal: Journal of Clinical and Translational Hepatology
doi: 10.14218/JCTH.2025.00416
Figure Legend Snippet: (A) tSNE plot showing the four neutrophil-derived MDSC subtypes. (B) The top five expressed genes of IL1RN + MDSCs in the four neutrophil-derived MDSC subsets. (C, D) Representative flow cytometry data and quantification graphs showing the proportion of IL1RA + MDSCs in LNN and LNP PDAC tissues. (E) Representative images of immunofluorescence staining of IL1RA + MDSCs in LNN and LNP PDAC tissues. Scale bar = 100 µm. (F) Trajectory of neutrophil-derived MDSCs along pseudotime in two-dimensional space. (G) Heatmap showing dynamic changes in gene expression along pseudotime. (H) Prognostic analysis of PDAC patients with low or high IL1RA + MDSC recruitment (stratified by the median IL1RA + MDSC proportion in all samples). Kaplan–Meier survival plots of 16 patients are shown. Data are presented as mean ± SD in (D). Statistical differences were assessed using a two-tailed Student’s t -test. LNN, lymph node-negative; LNP, lymph node-positive; PDAC, pancreatic ductal adenocarcinoma; MDSCs, myeloid-derived suppressor cells.
Techniques Used: Derivative Assay, Flow Cytometry, Immunofluorescence, Staining, Gene Expression, Two Tailed Test
Figure Legend Snippet: (A) Schematic illustration of IL1RN + MDSC–PDAC co-culture system. (B) Transwell migration assays in PANC-1 and ASPC-1 cells treated with normal medium (Control), IL1RA + MDSC-conditioned medium (IL1RA + MDSC CM), or IL1RA + MDSC CM + Axitinib. Scale bar = 50 µm. (C) Western blot analysis of EMT markers (N-cadherin, E-cadherin, ZO-1, Claudin-1) in PANC-1 cells under treatments identical to (B); β-actin was detected as a control. Grayscale was determined using ImageJ. (D) Relative expression of stemness-related mRNA after treatment with IL1RA + MDSC CM or IL1RA + MDSC CM + Axitinib. (E) Representative images of subcutaneous tumors in BALB/c nude mice from the PDX model: Group 1 (Control, tumor tissue only); Group 2 (tumor tissue co-implanted with IL1RN + MDSCs); Group 3 (tumor tissue co-implanted with IL1RN + MDSCs + Axitinib). (F) Excised tumor tissues from (E). (G, H) Tumor weights (G) and volumes (H) from the mouse model presented as bar graphs. (I) Representative H&E staining images of mouse pancreatic tumor tissues. Scale bar = 100 µm. Data are represented as mean ± SD of three independent biological replicates in (B) and four independent replicates in (F and G). Statistical significance was determined by one-way ANOVA with Dunnett’s post-hoc test: ns p ≥ 0.05; * p < 0.05; *** p < 0.001; **** p < 0.0001. PDAC, pancreatic ductal adenocarcinoma; MDSCs, myeloid-derived suppressor cells.
Techniques Used: Co-Culture Assay, Migration, Control, Western Blot, Expressing, Staining, Derivative Assay
Related Articles
Transferring:Article Title: IL1RA inhibits the progression of oral squamous cell carcinoma by mediating type Ⅰ interferon response. Article Snippet: Following centrifugation, protein samples immersed in 200 μl of the supernatant and 50 μl of 5 × Protein Sampling Buffer were heated at 100 ◦C, and subjected to gel electrophoresis using the One-Step PAGE Gel Fast Preparation Kit (Vazyme) at 60 V and 600 mA. .. Transferring on the Polyvinylidene fluoride (PVDF) membrane, it was immersed in 5 % skim milk, and incubated overnight at 4◦C with primary antibodies against β-Actin (#3700, CST, 1:1000), Article Title: IL1RA inhibits the progression of oral squamous cell carcinoma by mediating type Ⅰ interferon response Article Snippet: Following centrifugation, protein samples immersed in 200 μl of the supernatant and 50 μl of 5 × Protein Sampling Buffer were heated at 100 °C, and subjected to gel electrophoresis using the One-Step PAGE Gel Fast Preparation Kit (Vazyme) at 60 V and 600 mA. .. Transferring on the Polyvinylidene fluoride (PVDF) membrane, it was immersed in 5 % skim milk, and incubated overnight at 4°C with primary antibodies against β-Actin (#3700, CST, 1:1000), Membrane:Article Title: IL1RA inhibits the progression of oral squamous cell carcinoma by mediating type Ⅰ interferon response. Article Snippet: Following centrifugation, protein samples immersed in 200 μl of the supernatant and 50 μl of 5 × Protein Sampling Buffer were heated at 100 ◦C, and subjected to gel electrophoresis using the One-Step PAGE Gel Fast Preparation Kit (Vazyme) at 60 V and 600 mA. .. Transferring on the Polyvinylidene fluoride (PVDF) membrane, it was immersed in 5 % skim milk, and incubated overnight at 4◦C with primary antibodies against β-Actin (#3700, CST, 1:1000), Article Title: IL1RA inhibits the progression of oral squamous cell carcinoma by mediating type Ⅰ interferon response Article Snippet: Following centrifugation, protein samples immersed in 200 μl of the supernatant and 50 μl of 5 × Protein Sampling Buffer were heated at 100 °C, and subjected to gel electrophoresis using the One-Step PAGE Gel Fast Preparation Kit (Vazyme) at 60 V and 600 mA. .. Transferring on the Polyvinylidene fluoride (PVDF) membrane, it was immersed in 5 % skim milk, and incubated overnight at 4°C with primary antibodies against β-Actin (#3700, CST, 1:1000), Incubation:Article Title: IL1RA inhibits the progression of oral squamous cell carcinoma by mediating type Ⅰ interferon response. Article Snippet: Following centrifugation, protein samples immersed in 200 μl of the supernatant and 50 μl of 5 × Protein Sampling Buffer were heated at 100 ◦C, and subjected to gel electrophoresis using the One-Step PAGE Gel Fast Preparation Kit (Vazyme) at 60 V and 600 mA. .. Transferring on the Polyvinylidene fluoride (PVDF) membrane, it was immersed in 5 % skim milk, and incubated overnight at 4◦C with primary antibodies against β-Actin (#3700, CST, 1:1000), Article Title: IL1RA inhibits the progression of oral squamous cell carcinoma by mediating type Ⅰ interferon response Article Snippet: Following centrifugation, protein samples immersed in 200 μl of the supernatant and 50 μl of 5 × Protein Sampling Buffer were heated at 100 °C, and subjected to gel electrophoresis using the One-Step PAGE Gel Fast Preparation Kit (Vazyme) at 60 V and 600 mA. .. Transferring on the Polyvinylidene fluoride (PVDF) membrane, it was immersed in 5 % skim milk, and incubated overnight at 4°C with primary antibodies against β-Actin (#3700, CST, 1:1000), |