il1ra Search Results


il 1β  (MedChemExpress)
93
MedChemExpress il 1β
Hypercapnia increased the expression <t>of</t> <t>IL-1β</t> in the hypoxic blood in vivo and in vitro (n=4). (A) There was an interaction effect between hypoxia treatment and hypercapnia treatment (P<0.01). (B) Simple effects analyses revealed increased IL-1β expression in the hypoxemia group ( ** P<0.01), but not in the hypercapnia group (P>0.05) compared with the Sham group. The expression levels of IL-1β were the highest in the HH group in comparison with the hypoxemia group ( ** P<0.01) and the hypercapnia group ( ** P<0.01). (C) There was an interaction effect between 0.2% O 2 treatment and 10% CO 2 treatment. (D) Simple effects analyses revealed increased IL-1β expression in the hypoxia group ( ** P<0.01), but not in the HC group (P>0.05) compared with the control group. The expression levels of IL-1β were the highest in the hypoxia + HC group compared with the hypoxia group ( ** P<0.01) and the HC group ( ** P<0.01). The concentrations of O 2 and CO 2 in the air were 21 and 0.03%, respectively. IL-1β, interleukin-1β; HH, hypercapnia + hypoxemia; ns, non‑significant; HC, high concentration of carbon dioxide.
Il 1β, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il1ra/IL-1RA%2FIL-1RN%2C+Human/pmc07307827-78-8-15
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recombinant human il 1ra  (R&D Systems)
94
R&D Systems recombinant human il 1ra
Hypercapnia increased the expression <t>of</t> <t>IL-1β</t> in the hypoxic blood in vivo and in vitro (n=4). (A) There was an interaction effect between hypoxia treatment and hypercapnia treatment (P<0.01). (B) Simple effects analyses revealed increased IL-1β expression in the hypoxemia group ( ** P<0.01), but not in the hypercapnia group (P>0.05) compared with the Sham group. The expression levels of IL-1β were the highest in the HH group in comparison with the hypoxemia group ( ** P<0.01) and the hypercapnia group ( ** P<0.01). (C) There was an interaction effect between 0.2% O 2 treatment and 10% CO 2 treatment. (D) Simple effects analyses revealed increased IL-1β expression in the hypoxia group ( ** P<0.01), but not in the HC group (P>0.05) compared with the control group. The expression levels of IL-1β were the highest in the hypoxia + HC group compared with the hypoxia group ( ** P<0.01) and the HC group ( ** P<0.01). The concentrations of O 2 and CO 2 in the air were 21 and 0.03%, respectively. IL-1β, interleukin-1β; HH, hypercapnia + hypoxemia; ns, non‑significant; HC, high concentration of carbon dioxide.
Recombinant Human Il 1ra, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il1ra/Recombinant+Human+IL-1ra%2FIL-1F3+Protein/10__1523_slash_jneurosci__20___23___j0004__2000-48-0-10
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human il 1rα il 1f3 quantikine elisa kit  (R&D Systems)
94
R&D Systems human il 1rα il 1f3 quantikine elisa kit
Hypercapnia increased the expression <t>of</t> <t>IL-1β</t> in the hypoxic blood in vivo and in vitro (n=4). (A) There was an interaction effect between hypoxia treatment and hypercapnia treatment (P<0.01). (B) Simple effects analyses revealed increased IL-1β expression in the hypoxemia group ( ** P<0.01), but not in the hypercapnia group (P>0.05) compared with the Sham group. The expression levels of IL-1β were the highest in the HH group in comparison with the hypoxemia group ( ** P<0.01) and the hypercapnia group ( ** P<0.01). (C) There was an interaction effect between 0.2% O 2 treatment and 10% CO 2 treatment. (D) Simple effects analyses revealed increased IL-1β expression in the hypoxia group ( ** P<0.01), but not in the HC group (P>0.05) compared with the control group. The expression levels of IL-1β were the highest in the hypoxia + HC group compared with the hypoxia group ( ** P<0.01) and the HC group ( ** P<0.01). The concentrations of O 2 and CO 2 in the air were 21 and 0.03%, respectively. IL-1β, interleukin-1β; HH, hypercapnia + hypoxemia; ns, non‑significant; HC, high concentration of carbon dioxide.
Human Il 1rα Il 1f3 Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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human il 1ra quantikine immunoassay  (R&D Systems)
90
R&D Systems human il 1ra quantikine immunoassay
Hypercapnia increased the expression <t>of</t> <t>IL-1β</t> in the hypoxic blood in vivo and in vitro (n=4). (A) There was an interaction effect between hypoxia treatment and hypercapnia treatment (P<0.01). (B) Simple effects analyses revealed increased IL-1β expression in the hypoxemia group ( ** P<0.01), but not in the hypercapnia group (P>0.05) compared with the Sham group. The expression levels of IL-1β were the highest in the HH group in comparison with the hypoxemia group ( ** P<0.01) and the hypercapnia group ( ** P<0.01). (C) There was an interaction effect between 0.2% O 2 treatment and 10% CO 2 treatment. (D) Simple effects analyses revealed increased IL-1β expression in the hypoxia group ( ** P<0.01), but not in the HC group (P>0.05) compared with the control group. The expression levels of IL-1β were the highest in the hypoxia + HC group compared with the hypoxia group ( ** P<0.01) and the HC group ( ** P<0.01). The concentrations of O 2 and CO 2 in the air were 21 and 0.03%, respectively. IL-1β, interleukin-1β; HH, hypercapnia + hypoxemia; ns, non‑significant; HC, high concentration of carbon dioxide.
Human Il 1ra Quantikine Immunoassay, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il1ra/Quantikine+Immunoassay+Control+Set+1000+for+Human+IL-1ra/pmc00506965-133-19-27
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il 1rn  (R&D Systems)
94
R&D Systems il 1rn
Hypercapnia increased the expression <t>of</t> <t>IL-1β</t> in the hypoxic blood in vivo and in vitro (n=4). (A) There was an interaction effect between hypoxia treatment and hypercapnia treatment (P<0.01). (B) Simple effects analyses revealed increased IL-1β expression in the hypoxemia group ( ** P<0.01), but not in the hypercapnia group (P>0.05) compared with the Sham group. The expression levels of IL-1β were the highest in the HH group in comparison with the hypoxemia group ( ** P<0.01) and the hypercapnia group ( ** P<0.01). (C) There was an interaction effect between 0.2% O 2 treatment and 10% CO 2 treatment. (D) Simple effects analyses revealed increased IL-1β expression in the hypoxia group ( ** P<0.01), but not in the HC group (P>0.05) compared with the control group. The expression levels of IL-1β were the highest in the hypoxia + HC group compared with the hypoxia group ( ** P<0.01) and the HC group ( ** P<0.01). The concentrations of O 2 and CO 2 in the air were 21 and 0.03%, respectively. IL-1β, interleukin-1β; HH, hypercapnia + hypoxemia; ns, non‑significant; HC, high concentration of carbon dioxide.
Il 1rn, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il1ra/Human+IL-1ra%2FIL-1F3+Quantikine+ELISA+Kit/pm41352784-143-12-13
Average 94 stars, based on 1 article reviews
il 1rn - by Bioz Stars, 2026-10
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il 1ra protein  (R&D Systems)
94
R&D Systems il 1ra protein
Hypercapnia increased the expression <t>of</t> <t>IL-1β</t> in the hypoxic blood in vivo and in vitro (n=4). (A) There was an interaction effect between hypoxia treatment and hypercapnia treatment (P<0.01). (B) Simple effects analyses revealed increased IL-1β expression in the hypoxemia group ( ** P<0.01), but not in the hypercapnia group (P>0.05) compared with the Sham group. The expression levels of IL-1β were the highest in the HH group in comparison with the hypoxemia group ( ** P<0.01) and the hypercapnia group ( ** P<0.01). (C) There was an interaction effect between 0.2% O 2 treatment and 10% CO 2 treatment. (D) Simple effects analyses revealed increased IL-1β expression in the hypoxia group ( ** P<0.01), but not in the HC group (P>0.05) compared with the control group. The expression levels of IL-1β were the highest in the hypoxia + HC group compared with the hypoxia group ( ** P<0.01) and the HC group ( ** P<0.01). The concentrations of O 2 and CO 2 in the air were 21 and 0.03%, respectively. IL-1β, interleukin-1β; HH, hypercapnia + hypoxemia; ns, non‑significant; HC, high concentration of carbon dioxide.
Il 1ra Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il1ra/Recombinant+Human+IL-1ra%2FIL-1F3+Protein%2C+CF/pmc07041122-97-6-11
Average 94 stars, based on 1 article reviews
il 1ra protein - by Bioz Stars, 2026-10
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human il 1ra il 1f3 duoset elisa  (R&D Systems)
94
R&D Systems human il 1ra il 1f3 duoset elisa
Hypercapnia increased the expression <t>of</t> <t>IL-1β</t> in the hypoxic blood in vivo and in vitro (n=4). (A) There was an interaction effect between hypoxia treatment and hypercapnia treatment (P<0.01). (B) Simple effects analyses revealed increased IL-1β expression in the hypoxemia group ( ** P<0.01), but not in the hypercapnia group (P>0.05) compared with the Sham group. The expression levels of IL-1β were the highest in the HH group in comparison with the hypoxemia group ( ** P<0.01) and the hypercapnia group ( ** P<0.01). (C) There was an interaction effect between 0.2% O 2 treatment and 10% CO 2 treatment. (D) Simple effects analyses revealed increased IL-1β expression in the hypoxia group ( ** P<0.01), but not in the HC group (P>0.05) compared with the control group. The expression levels of IL-1β were the highest in the hypoxia + HC group compared with the hypoxia group ( ** P<0.01) and the HC group ( ** P<0.01). The concentrations of O 2 and CO 2 in the air were 21 and 0.03%, respectively. IL-1β, interleukin-1β; HH, hypercapnia + hypoxemia; ns, non‑significant; HC, high concentration of carbon dioxide.
Human Il 1ra Il 1f3 Duoset Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il1ra/Human+IL-1ra%2FIL-1F3+DuoSet+ELISA/pm41889175-194-25-30
Average 94 stars, based on 1 article reviews
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human il 1ra duoset elisa kit  (R&D Systems)
95
R&D Systems human il 1ra duoset elisa kit
Hypercapnia increased the expression <t>of</t> <t>IL-1β</t> in the hypoxic blood in vivo and in vitro (n=4). (A) There was an interaction effect between hypoxia treatment and hypercapnia treatment (P<0.01). (B) Simple effects analyses revealed increased IL-1β expression in the hypoxemia group ( ** P<0.01), but not in the hypercapnia group (P>0.05) compared with the Sham group. The expression levels of IL-1β were the highest in the HH group in comparison with the hypoxemia group ( ** P<0.01) and the hypercapnia group ( ** P<0.01). (C) There was an interaction effect between 0.2% O 2 treatment and 10% CO 2 treatment. (D) Simple effects analyses revealed increased IL-1β expression in the hypoxia group ( ** P<0.01), but not in the HC group (P>0.05) compared with the control group. The expression levels of IL-1β were the highest in the hypoxia + HC group compared with the hypoxia group ( ** P<0.01) and the HC group ( ** P<0.01). The concentrations of O 2 and CO 2 in the air were 21 and 0.03%, respectively. IL-1β, interleukin-1β; HH, hypercapnia + hypoxemia; ns, non‑significant; HC, high concentration of carbon dioxide.
Human Il 1ra Duoset Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti il 1r1  (Proteintech)
94
Proteintech anti il 1r1
Hypercapnia increased the expression <t>of</t> <t>IL-1β</t> in the hypoxic blood in vivo and in vitro (n=4). (A) There was an interaction effect between hypoxia treatment and hypercapnia treatment (P<0.01). (B) Simple effects analyses revealed increased IL-1β expression in the hypoxemia group ( ** P<0.01), but not in the hypercapnia group (P>0.05) compared with the Sham group. The expression levels of IL-1β were the highest in the HH group in comparison with the hypoxemia group ( ** P<0.01) and the hypercapnia group ( ** P<0.01). (C) There was an interaction effect between 0.2% O 2 treatment and 10% CO 2 treatment. (D) Simple effects analyses revealed increased IL-1β expression in the hypoxia group ( ** P<0.01), but not in the HC group (P>0.05) compared with the control group. The expression levels of IL-1β were the highest in the hypoxia + HC group compared with the hypoxia group ( ** P<0.01) and the HC group ( ** P<0.01). The concentrations of O 2 and CO 2 in the air were 21 and 0.03%, respectively. IL-1β, interleukin-1β; HH, hypercapnia + hypoxemia; ns, non‑significant; HC, high concentration of carbon dioxide.
Anti Il 1r1, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il1ra/Human+IL-1R1+ELISA+Kit/pmc11920578-80-31-33
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anti human il 1ra  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology anti human il 1ra
Hypercapnia increased the expression <t>of</t> <t>IL-1β</t> in the hypoxic blood in vivo and in vitro (n=4). (A) There was an interaction effect between hypoxia treatment and hypercapnia treatment (P<0.01). (B) Simple effects analyses revealed increased IL-1β expression in the hypoxemia group ( ** P<0.01), but not in the hypercapnia group (P>0.05) compared with the Sham group. The expression levels of IL-1β were the highest in the HH group in comparison with the hypoxemia group ( ** P<0.01) and the hypercapnia group ( ** P<0.01). (C) There was an interaction effect between 0.2% O 2 treatment and 10% CO 2 treatment. (D) Simple effects analyses revealed increased IL-1β expression in the hypoxia group ( ** P<0.01), but not in the HC group (P>0.05) compared with the control group. The expression levels of IL-1β were the highest in the hypoxia + HC group compared with the hypoxia group ( ** P<0.01) and the HC group ( ** P<0.01). The concentrations of O 2 and CO 2 in the air were 21 and 0.03%, respectively. IL-1β, interleukin-1β; HH, hypercapnia + hypoxemia; ns, non‑significant; HC, high concentration of carbon dioxide.
Anti Human Il 1ra, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit polyclonal anti iba1 antibody  (Rockland Immunochemicals)
93
Rockland Immunochemicals rabbit polyclonal anti iba1 antibody
Early-stage tissue evaluation after neurite bundle-derived artificial nerve transplantation. a , b Immunohistochemistry images with anti-NFH and anti-CD31 antibodies. Dotted arrows indicate the proximal side of the intact nerve areas, and solid arrows indicate the NFH- and CD31-positive regeneration tip in the reconstructed tissue. Scale bars = 1 mm. c , d Quantitative comparison of axonal extension distance was indicated by NFH-positive areas and that of vascular elongation distance was demonstrated by CD31-positive areas at 1 and 2 weeks after transplantation. Significant differences between groups elongation distances were observed in 2-week samples only. e , f <t>Iba1</t> immunohistochemistry image of the proximal part of the regenerated tissue at 2 weeks after transplantation (e). Scale bars = 1 mm. Quantitative analysis of <t>Iba1-positive</t> areas demonstrated that a larger number of macrophages aggregated in the Auto, motor TP and sensory TP (f). ( n = 3). * p < 0.05, ** p < 0.01, N.S. = not significant. Data are represented as the mean ± SEM
Rabbit Polyclonal Anti Iba1 Antibody, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il1ra/IL-1+Receptor+Antagonist+Antibody/pmc10863150-342-109-121
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presence absence  (MedChemExpress)
94
MedChemExpress presence absence
Early-stage tissue evaluation after neurite bundle-derived artificial nerve transplantation. a , b Immunohistochemistry images with anti-NFH and anti-CD31 antibodies. Dotted arrows indicate the proximal side of the intact nerve areas, and solid arrows indicate the NFH- and CD31-positive regeneration tip in the reconstructed tissue. Scale bars = 1 mm. c , d Quantitative comparison of axonal extension distance was indicated by NFH-positive areas and that of vascular elongation distance was demonstrated by CD31-positive areas at 1 and 2 weeks after transplantation. Significant differences between groups elongation distances were observed in 2-week samples only. e , f <t>Iba1</t> immunohistochemistry image of the proximal part of the regenerated tissue at 2 weeks after transplantation (e). Scale bars = 1 mm. Quantitative analysis of <t>Iba1-positive</t> areas demonstrated that a larger number of macrophages aggregated in the Auto, motor TP and sensory TP (f). ( n = 3). * p < 0.05, ** p < 0.01, N.S. = not significant. Data are represented as the mean ± SEM
Presence Absence, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il1ra/IL-1RA%2FIL-1F3%2C+Porcine/pmc12597836-58-13-22
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Image Search Results


Hypercapnia increased the expression of IL-1β in the hypoxic blood in vivo and in vitro (n=4). (A) There was an interaction effect between hypoxia treatment and hypercapnia treatment (P<0.01). (B) Simple effects analyses revealed increased IL-1β expression in the hypoxemia group ( ** P<0.01), but not in the hypercapnia group (P>0.05) compared with the Sham group. The expression levels of IL-1β were the highest in the HH group in comparison with the hypoxemia group ( ** P<0.01) and the hypercapnia group ( ** P<0.01). (C) There was an interaction effect between 0.2% O 2 treatment and 10% CO 2 treatment. (D) Simple effects analyses revealed increased IL-1β expression in the hypoxia group ( ** P<0.01), but not in the HC group (P>0.05) compared with the control group. The expression levels of IL-1β were the highest in the hypoxia + HC group compared with the hypoxia group ( ** P<0.01) and the HC group ( ** P<0.01). The concentrations of O 2 and CO 2 in the air were 21 and 0.03%, respectively. IL-1β, interleukin-1β; HH, hypercapnia + hypoxemia; ns, non‑significant; HC, high concentration of carbon dioxide.

Journal: International Journal of Molecular Medicine

Article Title: Hypercapnia exacerbates the disruption of the blood‑brain barrier by inducing interleukin‑1β overproduction in the blood of hypoxemic adult rats

doi: 10.3892/ijmm.2020.4604

Figure Lengend Snippet: Hypercapnia increased the expression of IL-1β in the hypoxic blood in vivo and in vitro (n=4). (A) There was an interaction effect between hypoxia treatment and hypercapnia treatment (P<0.01). (B) Simple effects analyses revealed increased IL-1β expression in the hypoxemia group ( ** P<0.01), but not in the hypercapnia group (P>0.05) compared with the Sham group. The expression levels of IL-1β were the highest in the HH group in comparison with the hypoxemia group ( ** P<0.01) and the hypercapnia group ( ** P<0.01). (C) There was an interaction effect between 0.2% O 2 treatment and 10% CO 2 treatment. (D) Simple effects analyses revealed increased IL-1β expression in the hypoxia group ( ** P<0.01), but not in the HC group (P>0.05) compared with the control group. The expression levels of IL-1β were the highest in the hypoxia + HC group compared with the hypoxia group ( ** P<0.01) and the HC group ( ** P<0.01). The concentrations of O 2 and CO 2 in the air were 21 and 0.03%, respectively. IL-1β, interleukin-1β; HH, hypercapnia + hypoxemia; ns, non‑significant; HC, high concentration of carbon dioxide.

Article Snippet: The IL-1β + IL-1Ra group was treated with IL-1β (40 ng/ml) and IL-1Ra (40 ng/ml; MedChemExpress; cat. no. HY-P7029).

Techniques: Expressing, In Vivo, In Vitro, Comparison, Control, Concentration Assay

IL-1β treatment increased IL-1R1 and p-IRAK-1 expression in RBECs (n=4). (A) Immunoreactive bands of IL-1R1 (80 kDa) and β-actin (42 kDa). (B) The bar graph shows increased IL-1R1 expression in the IL-1β group compared with that in the control group ( ** P<0.01). (C) Immunofluorescence images showing the expression of CD31 + RBECs (a and d, green), IL-1R1 (b and e, red), and the co-localization of IL-1R1 and RBECs (c and f). Enhanced IL-1R1 immunofluorescence was evident in the IL‑1β group compared with the control group. Scale bars (a-f): 10 μ m. (D) Immunoreactive bands of p-RIAK-1 (77 kDa), t-IRAK-1 (77 kDa) and β-actin (42 kDa). (E) The bar graph shows increased p-IRAK-1 expression in the IL-1β group compared with the control group ( ** P<0.01). The protein expression of p‑IRAK‑1 was significantly suppressed with IL‑1Ra treatment ( * P<0.05). RBECs, rat brain capillary endothelial cells; IL-1β, interleukin-1β; IL‑1R, interleukin‑1 receptor; IL‑1Ra, interleukin‑1 receptor antagonist; HC, high concentration of carbon dioxide; ns, non‑significant.

Journal: International Journal of Molecular Medicine

Article Title: Hypercapnia exacerbates the disruption of the blood‑brain barrier by inducing interleukin‑1β overproduction in the blood of hypoxemic adult rats

doi: 10.3892/ijmm.2020.4604

Figure Lengend Snippet: IL-1β treatment increased IL-1R1 and p-IRAK-1 expression in RBECs (n=4). (A) Immunoreactive bands of IL-1R1 (80 kDa) and β-actin (42 kDa). (B) The bar graph shows increased IL-1R1 expression in the IL-1β group compared with that in the control group ( ** P<0.01). (C) Immunofluorescence images showing the expression of CD31 + RBECs (a and d, green), IL-1R1 (b and e, red), and the co-localization of IL-1R1 and RBECs (c and f). Enhanced IL-1R1 immunofluorescence was evident in the IL‑1β group compared with the control group. Scale bars (a-f): 10 μ m. (D) Immunoreactive bands of p-RIAK-1 (77 kDa), t-IRAK-1 (77 kDa) and β-actin (42 kDa). (E) The bar graph shows increased p-IRAK-1 expression in the IL-1β group compared with the control group ( ** P<0.01). The protein expression of p‑IRAK‑1 was significantly suppressed with IL‑1Ra treatment ( * P<0.05). RBECs, rat brain capillary endothelial cells; IL-1β, interleukin-1β; IL‑1R, interleukin‑1 receptor; IL‑1Ra, interleukin‑1 receptor antagonist; HC, high concentration of carbon dioxide; ns, non‑significant.

Article Snippet: The IL-1β + IL-1Ra group was treated with IL-1β (40 ng/ml) and IL-1Ra (40 ng/ml; MedChemExpress; cat. no. HY-P7029).

Techniques: Expressing, Control, Immunofluorescence, Concentration Assay

IL-1β treatment decreased the expression of tight junctional proteins in RBECs (n=4). (A) Immunoreactive bands of ZO-1 (220 kDa), occludin (59 kDa), claudin-5 (23 kDa) and β-actin (42 kDa). (B) There was decreased tight junctional protein expression in the IL-1β group compared with the control group (ZO-1: ** P<0.01; occludin: ** P<0.01; and claudin-5: ** P<0.01). The expression of tight junctional proteins was significantly upregulated following IL-1Ra treatment (ZO-1: ** P<0.01; occludin: ** P<0.01; and claudin-5: * P<0.05). RBECs, rat brain capillary endothelial cells; ZO-1, zonula occludens-1; IL-1β, interleukin-1β; IL‑1Ra, interleukin‑1 receptor antagonist; ns, non‑significant.

Journal: International Journal of Molecular Medicine

Article Title: Hypercapnia exacerbates the disruption of the blood‑brain barrier by inducing interleukin‑1β overproduction in the blood of hypoxemic adult rats

doi: 10.3892/ijmm.2020.4604

Figure Lengend Snippet: IL-1β treatment decreased the expression of tight junctional proteins in RBECs (n=4). (A) Immunoreactive bands of ZO-1 (220 kDa), occludin (59 kDa), claudin-5 (23 kDa) and β-actin (42 kDa). (B) There was decreased tight junctional protein expression in the IL-1β group compared with the control group (ZO-1: ** P<0.01; occludin: ** P<0.01; and claudin-5: ** P<0.01). The expression of tight junctional proteins was significantly upregulated following IL-1Ra treatment (ZO-1: ** P<0.01; occludin: ** P<0.01; and claudin-5: * P<0.05). RBECs, rat brain capillary endothelial cells; ZO-1, zonula occludens-1; IL-1β, interleukin-1β; IL‑1Ra, interleukin‑1 receptor antagonist; ns, non‑significant.

Article Snippet: The IL-1β + IL-1Ra group was treated with IL-1β (40 ng/ml) and IL-1Ra (40 ng/ml; MedChemExpress; cat. no. HY-P7029).

Techniques: Expressing, Control

Early-stage tissue evaluation after neurite bundle-derived artificial nerve transplantation. a , b Immunohistochemistry images with anti-NFH and anti-CD31 antibodies. Dotted arrows indicate the proximal side of the intact nerve areas, and solid arrows indicate the NFH- and CD31-positive regeneration tip in the reconstructed tissue. Scale bars = 1 mm. c , d Quantitative comparison of axonal extension distance was indicated by NFH-positive areas and that of vascular elongation distance was demonstrated by CD31-positive areas at 1 and 2 weeks after transplantation. Significant differences between groups elongation distances were observed in 2-week samples only. e , f Iba1 immunohistochemistry image of the proximal part of the regenerated tissue at 2 weeks after transplantation (e). Scale bars = 1 mm. Quantitative analysis of Iba1-positive areas demonstrated that a larger number of macrophages aggregated in the Auto, motor TP and sensory TP (f). ( n = 3). * p < 0.05, ** p < 0.01, N.S. = not significant. Data are represented as the mean ± SEM

Journal: Inflammation and Regeneration

Article Title: Novel artificial nerve transplantation of human iPSC-derived neurite bundles enhanced nerve regeneration after peripheral nerve injury

doi: 10.1186/s41232-024-00319-4

Figure Lengend Snippet: Early-stage tissue evaluation after neurite bundle-derived artificial nerve transplantation. a , b Immunohistochemistry images with anti-NFH and anti-CD31 antibodies. Dotted arrows indicate the proximal side of the intact nerve areas, and solid arrows indicate the NFH- and CD31-positive regeneration tip in the reconstructed tissue. Scale bars = 1 mm. c , d Quantitative comparison of axonal extension distance was indicated by NFH-positive areas and that of vascular elongation distance was demonstrated by CD31-positive areas at 1 and 2 weeks after transplantation. Significant differences between groups elongation distances were observed in 2-week samples only. e , f Iba1 immunohistochemistry image of the proximal part of the regenerated tissue at 2 weeks after transplantation (e). Scale bars = 1 mm. Quantitative analysis of Iba1-positive areas demonstrated that a larger number of macrophages aggregated in the Auto, motor TP and sensory TP (f). ( n = 3). * p < 0.05, ** p < 0.01, N.S. = not significant. Data are represented as the mean ± SEM

Article Snippet: The primary antibodies used in this study were human polyclonal anti-pan-ELAVL (ELAV-like protein 2/3/4) antibody (1:2000, kindly provided from Prof. Robert Darnell, Rockefeller University), rabbit polyclonal anti-TrkA antibody (1:500, kindly provided from Prof. Louis F. Reichardt, UCSF), chicken polyclonal anti-TrkB antibody (1:500, kindly provided from Prof. Louis F. Reichardt, UCSF), goat polyclonal anti-TrkC antibody (AF373, 1:200, R&D systems), rabbit polyclonal anti-parvalbumin antibody (PV-28, 1:1000, Swant), rabbit polyclonal anti-CGRP antibody (BML-CA1134, 1:500, Enzo Life Sciences), Goat polyclonal anti-Choline acetyltransferase antibody (AB144P, 1:200, Chemicon), Mouse monoclonal anti-Islet1 and Islet2 antibody (39.4D5, 1:200, DSHB), rabbit polyclonal anti-Neurofilament heavy polypeptide antibody (ab8135, 1:500, Abcam), goat polyclonal anti-mouse and anti-rat CD31 (AF3628, 1:100, R&D), rabbit polyclonal anti-Iba1 antibody (GtX100042, 1:500, GeneTex), goat anti-GFP antibody (600–101-215, 1:1000, Rockland).

Techniques: Derivative Assay, Transplantation Assay, Immunohistochemistry, Comparison