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hpbmcs  (ATCC)


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    ATCC hpbmcs
    Hpbmcs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 894 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 894 article reviews
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    (a) Viability of S. purpuratus coelomocytes and human <t>peripheral</t> blood <t>mononuclear</t> cells <t>(PBMCs)</t> measured 24 h after exposure to a range of UVB doses. For coelomocytes, mean values ± the standard deviation of biological triplicates (n = 3) are shown. For PBMCs, mean values ± the standard deviation of technical triplicates are shown. Individual points are overlaid as white circles. (b) Example of a comet with the head and tail region indicated. (c) Average tail DNA percent and (d) average percent of damaged cells (the percent of cells with any amount of DNA damage) in S. purpuratus coelomocytes challenged with 1,000 mJ/cm 2 UVB at 1, 6, and 24 h post-exposure (red bars) versus control, untreated coelomocytes (purple bars). Error bars are mean values ± the standard deviation of biological triplicates (n = 3). Individual points are overlaid as white circles. Significant differences between control and UVB-treated cells were analyzed using a one-way ANOVA and post-hoc TukeyHSD test (***, adjusted p value < 1 x 10 -3 ).
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    (a) Viability of S. purpuratus coelomocytes and human peripheral blood mononuclear cells (PBMCs) measured 24 h after exposure to a range of UVB doses. For coelomocytes, mean values ± the standard deviation of biological triplicates (n = 3) are shown. For PBMCs, mean values ± the standard deviation of technical triplicates are shown. Individual points are overlaid as white circles. (b) Example of a comet with the head and tail region indicated. (c) Average tail DNA percent and (d) average percent of damaged cells (the percent of cells with any amount of DNA damage) in S. purpuratus coelomocytes challenged with 1,000 mJ/cm 2 UVB at 1, 6, and 24 h post-exposure (red bars) versus control, untreated coelomocytes (purple bars). Error bars are mean values ± the standard deviation of biological triplicates (n = 3). Individual points are overlaid as white circles. Significant differences between control and UVB-treated cells were analyzed using a one-way ANOVA and post-hoc TukeyHSD test (***, adjusted p value < 1 x 10 -3 ).

    Journal: bioRxiv

    Article Title: UVB-Induced Genotoxic Stress Activates the DNA Damage Response and Innate Immune Pathways in Sea Urchin Coelomocytes

    doi: 10.64898/2026.01.14.699502

    Figure Lengend Snippet: (a) Viability of S. purpuratus coelomocytes and human peripheral blood mononuclear cells (PBMCs) measured 24 h after exposure to a range of UVB doses. For coelomocytes, mean values ± the standard deviation of biological triplicates (n = 3) are shown. For PBMCs, mean values ± the standard deviation of technical triplicates are shown. Individual points are overlaid as white circles. (b) Example of a comet with the head and tail region indicated. (c) Average tail DNA percent and (d) average percent of damaged cells (the percent of cells with any amount of DNA damage) in S. purpuratus coelomocytes challenged with 1,000 mJ/cm 2 UVB at 1, 6, and 24 h post-exposure (red bars) versus control, untreated coelomocytes (purple bars). Error bars are mean values ± the standard deviation of biological triplicates (n = 3). Individual points are overlaid as white circles. Significant differences between control and UVB-treated cells were analyzed using a one-way ANOVA and post-hoc TukeyHSD test (***, adjusted p value < 1 x 10 -3 ).

    Article Snippet: In contrast, human peripheral blood mononuclear cells (PBMCs; ATCC PCS-800-011) demonstrated a sharp decline in viability following exposure to UVB with an LD 50 value of 871 mJ/cm 2 .

    Techniques: Standard Deviation, Control