Journal: Vaccine

Article Title: Cricket paralysis virus internal ribosome entry site-derived RNA promotes conventional vaccine efficacy by enhancing a balanced Th1/Th2 response

doi: 10.1016/j.vaccine.2019.07.070

Figure Lengend Snippet: The RNA adjuvant induces transcriptional profiles similar to those induced by poly(I:C) in hPBMCs. hPBMCs were stimulated with RNA adjuvant or poly(I:C) for 6-h and 24-h. Nil indicates PBS treatment. ( A ) Th1- and Th2-related cytokine production in the hPBMC supernatant was measured by multiplex analyses at 24-h post-stimulation. The data were statistically analyzed by ANOVA. The significance of differences between groups is indicated by different letters of the alphabet. p < 0.05. Data are presented as mean ± standard deviation ( n = 3). ( B ) Principal component analysis plots for all samples (three samples per group at 6- and 24-h post-treatment) (left) and scatter plots (right) of RNA-seq data (fold ratio ≥ 0.7). Nil indicates PBS-treated hPBMCs. ( C ) Heat maps showing clustering based on the signaling pathways associated with type 1 and 2 IFNs, TLR-Myd88), T cell activation, and leukocyte chemotaxis. ( D ) Simulated dynamics of the transcriptional network representing differentially expressed genes (DEGs). Interaction of proteins involved in the TLR signaling pathway, T cell activation, and proteins interacting with STAT1 and STAT3 (each square indicates a transcriptional factor, and each circle indicates a gene affected by a transcriptional factor). Red nodes are upregulated, and blue nodes are downregulated DEGs (fold-change ratio > 1). DEGs: differentially expressed genes, TLR: Toll-like receptor, Myd-88: myeloid differentiation primary response 88, Th: T helper cell, PBS: phosphate-buffered saline, hPBMC: human peripheral blood mononuclear cells, STAT: signal transducer and activator of transcription. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Human peripheral blood mononuclear cells (hPBMCs) obtained from Zen bio were cultured in RPMI 1640 medium (Hyclone Laboratories Inc, South Logan, UT, USA) supplemented with 10% heat-inactivated FBS (Life Technologies, Carlsbad, CA, USA), 2.05 mM L-glutamine (Hyclone Laboratories Inc, South Logan, UT, USA), and 1% Pen-Strep Glutamine (Gibco, Waltham, MA, USA).

Techniques: Adjuvant, Multiplex Assay, Standard Deviation, RNA Sequencing, Protein-Protein interactions, Activation Assay, Chemotaxis Assay, Saline

Journal: Vaccine

Article Title: Cricket paralysis virus internal ribosome entry site-derived RNA promotes conventional vaccine efficacy by enhancing a balanced Th1/Th2 response

doi: 10.1016/j.vaccine.2019.07.070

Figure Lengend Snippet: The RNA adjuvant mimics live-attenuated vaccine YF17D in transcription profiles associated with the innate immune response. Transcription profiles in response to live-attenuated vaccine YF17D in hPBMCs isolated from an immunized patient 7-days after vaccination (fold-change ≥ 2; p < 0.05; n = 30) and in response to the RNA adjuvant from hPBMCs at 6-h post-treatment. Induction and reduction profiles of genes related to innate immune responses ( A ) and type 1 IFN ( B ) from the RNA adjuvant (red) and YF17D (blue). ( C ) Comparison of IRF7 and STAT1 induction between RNA adjuvant (red) and YF17D (blue) treatment. Fold-change (log2) in expression in response treatment indicating the change in expression of each gene at 6-h post-treatment with the RNA adjuvant as compared PBS (RNA/Nil) and at 7-days post-immunization with YF17D as compared with that before immunization (YF17D/Nil). PBS: phosphate-buffered saline, hPBMC: human peripheral blood mononuclear cells, STAT: signal transducer and activator of transcription, IRF: interferon regulatory factor. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Human peripheral blood mononuclear cells (hPBMCs) obtained from Zen bio were cultured in RPMI 1640 medium (Hyclone Laboratories Inc, South Logan, UT, USA) supplemented with 10% heat-inactivated FBS (Life Technologies, Carlsbad, CA, USA), 2.05 mM L-glutamine (Hyclone Laboratories Inc, South Logan, UT, USA), and 1% Pen-Strep Glutamine (Gibco, Waltham, MA, USA).

Techniques: Adjuvant, Isolation, Comparison, Expressing, Saline

Journal: International Journal of Molecular Medicine

Article Title: Predictive DNA damage signaling for low-dose ionizing radiation

doi: 10.3892/ijmm.2024.5380

Figure Lengend Snippet: Expression levels of DNA damage repair proteins following IR. (A) IM-9, HuT 78 cells and hPBMCs were exposed to the indicated doses of radiation for 24 h. Representative western blots are shown. (B) hPBMCs were treated with the indicated doses of radiation for the indicated periods of time. Samples were analyzed using ELISA. Data represented the mean ± SD of three independent experiments and were fitted with non-linear regression using asymmetrical sigmoidal, five-parameter curves. IR, ionizing radiation; hPBMCs, human peripheral blood mononuclear cells; ATM, ataxia telangiectasia mutated; CHK2, checkpoint kinase 2; H2AX, H2A histone family member X. * P<0.05, ** P<0.01 and *** P<0.001 determined using two-way ANOVA.

Article Snippet: The IM-9, HuT 78, and hPBMCs cells were cultured in RPMI-1640 (Welgene, Inc.) supplemented with 10% FBS (Welgene, Inc.), penicillin (100 units/ml; Welgene, Inc.) and streptomycin (100 μg/ml; Welgene, Inc.).

Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Microorganisms

Article Title: Toxicological and Safety Pharmacological Profiling of the Anti-Infective and Anti-Inflammatory Peptide Pep19-2.5

doi: 10.3390/microorganisms10122412

Figure Lengend Snippet: Selected parameters for metabolic investigations using the chip-based cellular system (BIONAS).

Article Snippet: PBMC , Human peripheral blood mononuclear cells [hPBMCs] , ProBioGen, Berlin Germany.

Techniques:

Journal: Microorganisms

Article Title: Toxicological and Safety Pharmacological Profiling of the Anti-Infective and Anti-Inflammatory Peptide Pep19-2.5

doi: 10.3390/microorganisms10122412

Figure Lengend Snippet: ( A , B ) Time dependence of the respiration rate of human PBMC cells (human peripheral blood mononuclear cells) in the presence of different concentrations of Pep19-2.5 in the BIONAS chip-based system. Data are representative of three independent experiments.

Article Snippet: PBMC , Human peripheral blood mononuclear cells [hPBMCs] , ProBioGen, Berlin Germany.

Techniques: