Journal: International Journal of Molecular Sciences
Article Title: Curcuminoids Activate TET Enzymes and Increase DNA Hydroxymethylation and Active Demethylation in Leukemia Cells
doi: 10.3390/ijms27010310
Figure Lengend Snippet: Curcuminoids increase TET enzymatic activity in leukemia cells. Graded concentrations of curcumin (Cur) or DMC were used to treat U937 ( a , b ) and HL60 leukemia cells ( c , d ) for 48 h, followed by nuclear protein extraction and quantitation of TET activity, as described in the methods. The data represent the mean of 3 replicates ± SD. * indicates significant difference from the control at p < 0.05.
Article Snippet: Human promyeloid leukemia (HL60) and human monocytic leukemia (U937) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in RPMI-1640 medium (Sigma-Aldrich, Milwaukee, WI, USA) containing 10% fetal bovine serum (FBS) and 2.5 mM L-glutamine.
Techniques: Activity Assay, Protein Extraction, Quantitation Assay, Control